Anti-Ki-67

Polyclonal Antibody
Cat. # AB9260 pack size: 500 µL
Lot # 2147027 Store at 2-8°C

FOR RESEARCH USE ONLY Certificate of Analysis

page 1 of 2

Species Antibody Epitope/ Host Molecular

Applications Accession #
Cross-Reactivity Isotype Region Species Weight
WB, IH(P) H, M, R N/A N/A Rb ~345/395 kDa NM_002417.3

Background Storage and Handling

Ki67 antigen is the prototypic cell cycle related
nuclear protein, expressed by proliferating
cells in all phases of the active cell cycle (G1,
S, G2 and M phase). It is absent in resting
(G0) cells. Ki67 antibodies are useful in
establishing the cell growing fraction in
neoplasms (immunohistochemically quantified
by determining the number of Ki67 positive
cells among the total number of resting cells =
Ki67 index). In neoplastic tissues the
prognostic value is comparable to the tritiated
thymidine labelling index. The correlation
between low Ki67 index and histologically low
grade tumours is strong. Ki67 is routinely used
as a neuronal marker of cell cycling and
proliferation.
Presentation
Purified rabbit polyclonal. Liquid in PBS, pH7.6
containing 1% BSA with less than 0.1%
sodium azide.
Stable for 6 months at 2-8°C from date of
receipt.
Control
Positive Control: Breast carcinoma, lymph
node tissue, normal tonsil.
Quality Control Testing
Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed,
paraffin embedded tissue at 1:300 dilution for
10 minutes at room temperature. Staining of
formalin-fixed tissues requires boiling of
tissues in 10 mM citrate buffer, pH 6.0 for 10
minutes followed by cooling at room
temperature for 20 minutes.
Optimal Staining Without Epitope Retrieval:
Normal Tonsil

Specificity
Recognizes Ki-67
Species Cross-reactivity
Human and mouse. Expected to react with rat
based on sequence homology.
Immunogen
Synthetic peptide from human Ki-67
Molecular Weight
Predicted: 359 kDa. MW may be observed at
~345 or 395 kDa. Negative control staining in normal tonsil. Tissue was
Ki67 (PN: AB9260) staining in normal tonsil. Tissue was
treated with no antigen retrieval. Polyclonal antibody
Method of Purification treated with no antigen retrieval. Polyclonal antibody
diluent, IHC-Select® Detection with HRP-DAB. No signal
Rev.B/2010-06-21/AB9260CA/JM

diluted to 1:100, IHC-Select® Detection with HRP-DAB.

could be detected.
Immunoaffinity Purified Immunoreactivity is seeing as a strong nuclear signal
restricted to the germinal center

APPLICATION LEGEND: WB Western Blotting IP Immunoprecipitation IC Immunocytochemistry IF Immunofluorescence

IH Immunohistochemistry (Tissue) IH(P) Immunohistochemistry (Paraffin)
SPECIES LEGEND: H Human M Mouse R Rat Rb Rabbit WR Most Common Vertebrates
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Anti-Ki-67
Cat # AB9260
Lot # 2147027 page 2 of 2

Additional Research Applications

Immunocytochemistry: Confolcal fluorescent analysis of A431 Western Blot Analysis: A previous lot of this antibody was used in
cells using AB9260 rabbit pAB. Actin filaments were labeled western blot.
with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI.
(Nuclear staining).

Optimal working dilutions must be determined by the end user.

Immunocytochemistry:
Representative images from a previous lot.
Confolcal fluorescent analysis of A431 and HeLa cells using AB9260 rabbit pAB (Red). Actin filaments
have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).
(Nuclear staining)

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