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HEMATOLOGY 1 Hematopoiesis Notes 1
HEMATOLOGY 1 Hematopoiesis Notes 1
HEMATOPOIESIS
-most versatile; can develop into any cell type, can even develop into extra embryonic cells
(e.g. Placental cells)
- stem cell that has the potential to differentiate into any of the three germ layers: endoderm,
mesoderm, ectoderm
-stem cells that have the potential to differentiate into discrete cell types.
- e. g. multipotent blood stem cell (Hematopoietic stem cell): will differentiate into blood cells
PHASES
1. MESOBLASTIC PHASE
Begins: 19th day of embryonic dev’t.
Embryonic development:
Zygote-morula-blastula-gastrula
2. HEPATIC PHASE
Begins: 5 to 7 WOG
characterized by: Definitive Erythropoiesis, Granulopoiesis, monopoiesis, lymphopoiesis ,
megakaryopoiesis
- liver : major site of hematopoiesis during the second trimester of fetal life
- Hematopoiesis in the aorta-gonad-mesonephros region and the yolk sac disappear during
this stage.
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- Spleen, kidney, thymus, lymph nodes: also perform hematopoiesis along with the liver during
the hepatic stage.
3. MEDULLARY PHASE
Begins: end of 4th MOG / early 5th MOG
Medullary phase occurs in : medulla of bone; bone cavity
Characterized by: Increased Leukopoiesis and production of all adult blood cell types
BONE MARROW
Weight: 1500 g
Forms:
a. Red marrow: Hematopoietically active
b. Yellow marrow: Inactive, composed of adipocytes, Yellow color due to carotene of
adipocytes, also with macrophages and undifferentiated mesenchymal cells
** 5-7 yrs: yellow marrow begin to form in long bones (Process is known as
RETROGRESSION)
** 18 yrs: Red marrow restricted to: sternum (MAJOR SOURCE) , vertebrae, scapulae,
pelvis(iliac crest) , ribs, flat bones of skull, and proximal portion of the long bones( femur and
humerus)
-endothelial cells (BLOOD VESSEL CELLS: broad, flat, single continuous layer along surfaces
of blood vessels; regulate entry and exit of particles to and from BM )
-adipocytes (fat cells: Regulate BM volume; secrete cytokines and Growth factors to stimulate
hematopoiesis )
reticular adventitial cells (fibroblasts; supporting lattice for the developing blood cells.)
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2. Cytoplasmic characteristics:
a. Amount: Amount of cytoplasm increases upon maturity
b. Color: Cytoplasm loses basophilia ; darker blue to lighter blue, blue-gray, or pink (except:
Plasma cells)
c. Granules: blast cells generally do not contain granules; granules of granulated cells appear
on certain stages and specific granules appear later in the life cycle of maturing cells
3. Nuclear characteristics
a. Chromatin pattern:
-Immature cells: Fine, loose chromatin, smooth or homogeneous, fine, delicate, lacy or thready
b. Size:
-Mature: smaller
c. Nucleoli
-Mature:Invisible
d. Shape:
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ERYTHROPOIESIS
-The process of production and regulation of mature erythrocytes in the peripheral blood.
: characterized to undergo apoptosis (“burst” promoting activity) ; G0/ G1 phase of cell cycle-
not actively dividing
6. Pronormoblast
Aka: Proerythroblast; rubriblast
Size: 12-20 microns
Shape: ovoid
Cytoplasm: Thin rim of dark purplish hue ; scanty; moderate basophilia ; no granules T
Nucleus: fine chromatin pattern ; nucleoli 1-2/ 1-3
7.Basophilic Normoblast
Aka: Basophilic erythroblast; Prorubricyte
Size: 10-15 microns
8. Polychromatophilic normoblast
Aka: Polychromatic / Polychromic normoblast; polychromatophilic erythroblast; rubricyte
Size: 10-12 microns
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9. Orthochromatophilic normoblast
Aka: orthochromatic / Orthochromic normoblast; orthochromatophilic erythroblast;
metarubricyte
Size: 8-10 microns
* Loss of vimentin allow movement of nucleus towards periphery of cell until extrusion
10. Reticulocyte
Nucleus: none
* when supra vitally stained : may show precipitated ribosomes/ RNA fragments of precipitated
ribosomes
Nucleus: none
LEUKOPOIESIS
-production and regulation of production of granulocytes
3. Maturation pool- cells ready to be released but still undergoing nuclear maturation
(metamyelocytes, band neutrophils, and segmented neutrophils. )
HSC, CMP, GMP= cannot be distinguished under light microscope; must use flow cytomery
*Granulopoiesis
1. CFU-GEMM—-> CFU-GM
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4. Myeloblast
*earliets recognizable granulocyte precursor
a. Type I myeloblast
Nucleus: large oval- quadrangular; very fine / reticular nuclear chromatin ; 1-5/ 2-4 nucleoli
N:C ratio: 8:1- 4:1
b. Type II myeloblast:
Nucleus: large oval- round; very fine / reticular nuclear chromatin ; 1-5/ 2-4 nucleoli
N:C ratio: 8:1- 4:1
Nucleus: large oval-round ; darker staining reticular fine nuclear chromatin ; 1-5/ 2-4 nucleoli
N:C ratio: 8:1- 4:1
5. Promyelocyte
Nucleus: eccentric, round to oval with a peri nuclear halo/ hof (not in APML); smooth and
starts to clump ; 1-5 nucleoli
5. Myelocyte
* final stage capable of mitosis
* 6-17% of BM
* First stage where distinction between eosinophil , basophil, neutrophil can be seen
Cytoplasm: Blue-pink
5. Metamyelocyte
Aka: juvenile cell
* 3-20% of BM
* First stage where distinction between eosinophil , basophil, neutrophil can be seen
Cytoplasm: pink
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6. Band
Aka: stab cell
* 9-32% of BM
* First stage where distinction between eosinophil , basophil, neutrophil can be seen
Cytoplasm: pink
Nucleus: horseshoe/ sausage ; indentation more than half the diameter of nucleus (same d all
throughout ) ; highly clumped chromatin
7. Segmented neutrophils
Cytoplasm: pink
Half-life: 7 hours
a. Circulating pool
-major Bfs
b. Marginated pool
MONOCYTE MATURATION
1. CFU-GEMM—-> CFU-GM
Granules: none
Nucleus: large round to ovoid ; Naegeli type - chromatin similar to myeloblast ; Schilling type-
reticulum
N:C: 4: 1
4. Promonocyte
Cytoplasm: blue-gray
N:C: 3: 1- 2:1
5. Monocyte
Size: 15-20 microns
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LYMPHOPOIESIS
2. Lymphoblast
Size:15-20 microns
Nucleus: large oval nucleus with dark purple chromatin aggregates around nuclear periphery ;
1-2 nucleoli
N:C: 4:1
3. Prolymphocyte
Size: 15-18 microns
N:C: 3:1
4. Lymphocyte
Size: 6-10(SMALL) /15-20 (LGL)
MEGAKARYOPOIESIS
1. CFU-GEMM
2. CFU-MegE (***TPO)
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- Low concentrations
- Prevent apoptosis
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