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CH 1 Act 2 Lab Report Scaffolding
CH 1 Act 2 Lab Report Scaffolding
Adolat Beshimova
June 1, 2018
To find out if different sources of saliva will inhibit the growth of e.coli bacteria.
Hypothesis
dog saliva is known to have sterile properties and agents that directly attack the bacteria. The amylase
in the saliva of a dog can breakdown the cellular structure of a bacteria and The antibody IgA
(immunoglobulin A), which directly attacks bacteria in the saliva, due to these reasons we
hypothesize that the saliva of a dog is more effective than of human.
Materials
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● repeat this step all over again for the other 3 petri dishes
Data
Table 1
ampicillin 314 mm^2 314mm^2 254 mm^2 201 mm^2 271 mm^2
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Analysis
The data shows that there wasn’t any much effectiveness of dog saliva compared to
ampicillin, this might be due to human errors as well as other external factors. Some external factors
may include whether or not the dog ate just before the drool was obtained. Another reason why we
suspect it might have not worked is the mechanism of dog licking, when a dog licks a wound, it
removes all the bacteria of a wound. We did not stimulate this environment, on bacteria. We also
suspect the drool might have had some bacteria itself too. Experimental errors would be not washing
the incubator, not properly sterilizing the tools. Our control, ampicillin worked the best in terms of
zone of inhibition and human saliva did not significantly show inhibition according to data.
Ampicillin worked the best because it acts as an irreversible inhibitor of the enzyme transpeptidase,
which is needed by bacteria to make the cell wall. It inhibits the third and final stage of bacterial cell
wall synthesis in binary fission, which ultimately lead to the lysis of the cell that’s why ampicillin is
antibacterial.
Conclusion
Our research was inconclusive due to human errors. In terms of the result we obtained, it could be
noted that human salvia is not antibacterial in fact, according to our data, there were some instance
where the bacteria grew on the filter paper that contained the human saliva. If these steps were
repeated with more precautions and without human errors, there will be more accurate result.
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