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Determination of Alfacalcidol by UV
Determination of Alfacalcidol by UV
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Imran Tariq
University of the Punjab
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SUMMARY. The purpose of the study was to develop a new simple, accurate, precise and economic spec-
trophotometric method in UV-VIS region for the determination of alfacalcidol in bulk and pharmaceutical
tablet dosage form. The proposed method was validated in terms of linearity, accuracy, precision, limits of
detection and limits of quantification. It was found that Beer’s law was obeyed in range of 0.1-10 μg/mL
for alfacalcidol having correlation coefficient (R2) of 0.999. The method has been successfully applied for
the analysis of drugs in tablet formulation as well. The percent recovery was found from 99.69 to 100.11%
which indicate that the method is precise and reproducible. Limit of detection (LOD) and Limit of quan-
tification (LOQ) of drug were 0.09623 and 0.29161 μg/mL, respectively. Interday and intraday studies
showed good repeatability of the applied method. Percentage relative standard deviation values were
found less than 2% for proposed methods which illustrate the good precision. It was concluded that pro-
posed method was versatile, cheaper, accurate, selective, precise and rapid for the analysis of alfacalcidol.
Absorbance
to develop a new spectrophotometric method
which should be simple, accurate, precise and
reproducible for determination of alfacalcidol.
Instruments
Double beam Spectrophotometer UV-2550,
Shimadzu, Japan. Analytical balance Metler Absorbance
785
SAEED-UL-HASSAN S., TARIQ I., KARIM S., KHALIL-UR-REHMAN M., BASHIR S. & MURTAZA G.
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Latin American Journal of Pharmacy - 32 (5) - 2013
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SAEED-UL-HASSAN S., TARIQ I., KARIM S., KHALIL-UR-REHMAN M., BASHIR S. & MURTAZA G.
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