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Sample Type: 1.chemical Method
Sample Type: 1.chemical Method
Sample Type: 1.chemical Method
Sample used : whole blood or serum (ie, plasma with removal of fibrinogen ).
Because RBC have higher concentration of protein (eg, hemoglobin) than serum,
whereas serum has a higher water content and consequently more dissolved
glucose than does whole blood.
Arterial, capillary and venous blood have comparable glucose levels in a fasting
individual.
After meals venous levels are somewhat lower than capillary or arterial blood; a
common estimate is about 10%.
Measurement techniques
1.Chemical method
This method is based on the reducing property of glucose. But there are other compounds
like urea that show the same reducing properties. That is reason why this method can be
erratic at times
. The two most common employed enzymes are glucose oxidase and hexokinase..
I. CHEMICAL METHODS
A. Oxidation-Reduction Reaction
NADP as cofactor
NADPH (reduced product) is measured in 340 nm
More specific than Glucose Oxidase method due to G-6PO_4, which inhibits interfering substances
except when sample is hemolyzed
From 100 to 125 mg/dL (5.6 to 6.9 mmol/L) Impaired fasting glucose (pre-diabetes)
126 mg/dL (7.0 mmol/L) and above on more than one testing Diabetes
occasion
oral glucose tolerance test (OGTT) measures blood glucose after a person fasts at
least 8 hours and 2 hours after the person drinks a glucose-containing beverage. This
test can be used to diagnose diabetes and pre-diabetes.
1. food intake.
2. Infection
3. Stress; physical or psychological.
4. Exercise