Analytical Details

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ANALYTICAL DETAILS

Chromatographic system:

System: Agilent 1200 Series HPLC

Detector: Refractive Index Detector

Detector Temperature : 26̊̊˚C

Column Temperature : 85˚C

Column : Repromer Ca++ 7.8 X 300mm, 9-µm

Flow rate : 0.5mL/minute

Moile phase : 100% Milli Q Water

Sample preparation:

Weighed samples in 50ml volumetric flask added Milli Q water, sonicated for 10 min
made up to volume with Milli Q water, filtered through 0.45 µ filter paper and injected.

MANNITOL –CS-S89/FBR/07 1.0083g in 50ml Milli Q water

MANNITOL –CS-S89/WL/03 1.0098g in 50ml Milli Q water

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