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Pacific Oyster Physiological Response To Disease Under Variable Environmental Regimes
Pacific Oyster Physiological Response To Disease Under Variable Environmental Regimes
PCSGA 2010
Objective
How do environmental stressors, such as ocean
acidification (OA) and elevated temperature, affect
oyster larvae survival and physiology when
challenged by disease?
Experimental Plan
How do environmental changes affect the host-pathogen relationship?
High
OA
Temp.
Host
Pathogen
380 ppm
Normal Environment
1 2 3
Temperature & V. tubiashii
Assay Development
n=4
n=4
hatching
2h
5h
Time Post-Fertilization
veliger
17h
24h
Effects of OA on Development
=380 ppm
=840 ppm
Proportion Eggs in Cleavage
*
Effects of OA on Development
=380 ppm
=840 ppm
Proportion Larvae Hatched
*
Effects of OA on Development
A smaller proportion of larvae in elevated pCO2
develop at the “normal” rate
Implications for physiological anomalies and
calcification
1.0
0.8
Proportion Hatched
0.6
0.4
0.2
0.0
5 5 17 17 24 24
Time Post Fertilization (Hours)
Outline
Ocean acidification
Environmental stress and physiology
Temperature and the C. gigas-V. tubiashii
relationship
OA effects on C. gigas
Current work: OA & V. tubiashii
Hi
OA
T
OA & V. tubiashii
Study in progress in collaboration with NWFSC (P.
McElhany et al.)
Planned 3 weeks: spawning through settlement
4 pCO2 treatments
280, 380, 750, 2000 ppm
24-hour V. tubiashii exposure
Data collection:
Mortality
Physiology (gene expression)
Morphology & calcification
Genotype-linked survival
= calcified
OA & V. tubiashii = partially
calcified
Calcification at 48 hours post-fertilization = uncalcified
Conclusions
Ocean acidification has significant effects on C.
gigas larval development.
OA may inhibit and depress larval response to other
environmental stressors, including disease.
Elevated temperatures, such as 25oC, have effects
on the host-pathogen interaction.
Acknowledgements
Funding: Saltonstall-Kennedy Grant (NOAA),
NOAA Aquaculture Program student support
University of Washington
Moose O’Donnell
Sam White
Taylor Shellfish
Joth Davis
Ed Jones
Vicki Jones
NOAA Northwest Fisheries Science Center
Jason Miller
Mike Maher
Sarah Norberg