BIOGRAPHICAL SKETCH

NAME Hernani Cualing, MD POSITION TITLE

Associate Professor Of Oncology and Hematology
Medical Director, IHCFLOW, Inc.
EDUCATION/TRAINING
DEGREE
INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY
(if applicable)
University of the Philippines B.S. 1974 Premedical Sciences
University of the Philippines M.D. 1978 M.D.
A. Positions
1979 Medicine Internship, University of the Philippines
1980 Residency, Surgery
1985.87 Research Staff fellow, Dana-Farber Cancer Inst., Boston,MA
1987.90 AP/CP Pathology Residency,NCMC, SUNY Stony Brook, ,East Meadow, New York,
1990-91 Surgical Pathology fellowship, Nassau County Medical Center , SUNY Stony Brook;
1991.92 Hematopathology Fellow, Indiana University Medical Center, Riley Children’s Hospital, Indianapolis,
Indiana
1994-02 Medical Student Elective Director Diagnostic Hematopathology, Year IV UC-150146
1992-01 Assistant Professor of Pathology, University of Cincinnati Medical Center, Cincinnati, Ohio
2000-01 Associate Director, Hematopathology Fellowship Program, University of Cincinnati, 2001-02
Associate Professor, University of Cincinnati Medical Center, Cincinnati, Ohio
2002- Associate Professor of Oncology and Hematology, University of South Florida, Moffitt Cancer Center and
ResearcInstitute
2002-20010 Medical Director of Immunohistochemistry/Histology, Program of Pathology, Department of
Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida
2006- 2010 Director, Hematopathology fellowship Training Program, University of South Florida, Moffitt Cancer Center and
Res Institute
2010 - Univ of South Florida College of Medicine Dept of Pathology and Cell Biology, Consultant Medical Director, IHCFLOW,
Inc.

B. HONORS AND AWARDS

1969-1971 Scholastic Delegate to Physics, Chemistry, Biology Interscholastic Competitions

Academic Excellence-Top Academic Rank, Section Class # 1 of 26 classes
Senior High School, Ramon Magsaysay High School,
Manila, Philippines
1976-1977 President of Medical Student Society
University of the Philippines College of Medicine
Manila, Philippines
1989-1990 First Prize Award, Resident Presentation in Pathology,
“Zell Ballen” Patterns in Organ of Zukerkandl
Nassau Society of Pathologist
East Meadow, NY

1990-1991 Chief Resident, Department of Pathology

Nassau County Medical Center, SUNY at Stony Brook Teaching Hospital
East Meadow, NY

1992-1993 Finalist, American Society of Clinical Pathologist

Spring Resident Presentation Competition
Boston, MA

1994-1995 Biomedical Engineering Seed Grant Award-Top Score

I Image Analysis of Lymphomas
University of Cincinnati University- Seed Grant Competition
Cincinnati, Ohio

1997.2000 Biomedical Engineering Grant Award

“High Order Decision Theoretic Diagnosis of Lymphomas”
Whitaker Foundation ( National )

2000-2001 Stratmore’s Who’s Who of North America

2003-2010 Marquis Who’s Who, 58th, 60th, 61st, in North America

2006-2010 Marquis Who’s Who, 6th Edition in Science and Engineering,
Marquis Who’s Who, 9th Edition in Medicine & Health

2003-2004 Awardee- Top Overseas Researcher

University of the Philippines College of Medicine
Medical School Alumni Association
Twenty-Five Year Homecoming Celebration, Manila, Philippines

2006 First Prize Research Presentation by Mentored resident

“Mathematical Slope in BM recovery (Farah Khalil- Bone Marrow Quantitation )
Florida West Coast Assoc. of Pathologists/Pathology Alumni Assoc.
Longboat Key Conference, FL

2004-2005 Best Teacher of the Year Award

Awarded by AP/CP Pathology Residents
Department of Pathology
University of South Florida, Tampa, FL

2004.2007 Consultant: Immunohistochemistry and Histology Instrumentation

Council of Advisors for Health Care
New York, NY

2007.2008 Panelist: Small Business Innovations Research (SBIR/STTIR) Grant

National Science Foundation
Bethesda, MD

2007.2008 Mentor: Mentorship for Young scientist

International Society of Analytical Cytometry
Clinical Cytometry Society
Chicago, IL

2008 Consultant Adviser on Cutaneous Lymphomas

Advisory Board on Health Care
Eisai Corporation, Japan and USA

2007 FIRST Prize Research Retreat “Yoshinori Takahashi, D. Coppola, N. Matshushita, H. Cualing, M. Sun, Y.
Sato, C. Liang, J. Jung, J, Cheng, J. Mule, WJ Pledger, Hong Gang Wang. Bif-1 interacts with Beclin 1 through
UVRAG and regulates autophagy and tumorigenesis” 2007 Moffitt Scientific Retreat publication, Blaise Alfano
Conf.Center, Feb 24, 2007

2008 FIRST Prize Awardee Fellow Research Y.Takahashi, D.Coppola,

N. Matshushita, H. Cualing, M. Sato, C. Liang, J. Mule, WJ Pledger, Hong Gang Wang. H. Lee Mofttitt Cancer
Center Fellow Research Symphosium- Bif-1 in autophagy and tumorigenesis, Stabile Bldg.
Basic Science Division, May 19, 2008

Licensures and Certifications:

2002-2010 Florida Medical License # ME 84854, Expiration Jan 31, 2012, Active, Clear
1998 4th International Symposium on Predictive Oncology and Therapy, Nice, France
Lymph Node Pathology: ASCP Winter Seminar Certification, Orlando, FL
1994 T-cell Lymphomas-Comparison of Eastern and Western Experience: Hong Kong
1992 Ohio Medical License #35-06-4174, Inactive
1992 Diplomate of American Board of Pathology, Hematology Subspecialty
1992 Indiana University School of Medicine, Hematopathology Fellowship Training
1991 Diplomate of American Board of Pathology, Anatomic and Clinical Pathology
Lymph Node Pathology in the 90s (Bharat Nathwani), Washington DC
1990 New York Medical License #184512, Inactive
1990 Iowa Medical License #27672, Inactive
1991 ACLS(Advanced Cardiac Life Support) Certified, NY
1987 Lymphomas, Diagnosis from both sides of the Atlantic: Karl Lennert, Nassau, NY
ECFMG Certification #322-877-2
1978 National Medical Licensure Certification, Philippines

C. Selected Peer Reviewed Publications

H.Cualing Editor in Chief, Non-Neoplastic Hematopathology and Infections, Wiley: NJ, USA, 2011 in preparation.
Alekshun TJ, Rezania D, Ayala E, Cualing H, Sokol L.Skeletal muscle peripheral T-cell lymphoma. J Clin Oncol.
2008 Jan 20;26(3):501-3. IF: 13.598 PMID: 18202425
J Pidala, Cualing H, Pinilla J. Acute basophilic leukemia arising from chronic myelogenous leukemia with
development of t(7;8)(q34, q13) Cancer Genet Cytogenet. 2008 Apr 1;182 (1):46-9.
Raychaudhuri J, Cualing H, Haider K, Kharfan-Dabaja MA.Granulocytic sarcoma and chronic lymphocytic leukemia
of the gastrointestinal tract after allogeneic hematopoietic cell transplantation mimicking graft-versus-host disease.
Leuk Lymphoma. 2008 Feb;49(2):350-2. IF: 1.559 PMID: 18231925
Calder K, Khalil F, Schlauder S, Cualing H, Morgan M. Immunohistochemical expression of survivin in cutaneous
sebaceous lesions. Am J Dermatopathol. 2008 Dec;30(6):545-548. Pubmedid: 19033926.
Cualing H. Pathology of the Spleen. In Handbook of Hematologic Pathology, Schumacher H, Stass S. Eds. 53-73,
Marcel Dekker, Inc., New York, April 2000.: Book Chapter
Mojdeh Naghashpour, Hernani Cualing* Splenomegaly with sea-blue histiocytosis, dyslipidemia, and nephropathy in
a patient with lecithin-cholesterol acyltransferase deficiency: a clinicopathologic correlation, in press 2009
Metabolism Clinical and Experimental
Mojdeh Naghashpour, MD, PhD; Hernani D Cualing, MD; Margaret Szabunio, MD;
Marilyn M Bui, MD, PhD Hyaline-Vascular Castleman Disease: A Rare Cause of Solitary Subcutaneous Soft Tissue,
in press, 2009 The American Journal of Dermatopathology
Manuscript Number: AJD-D-09-00143 *
Condarco T, Sagatys E, Prakash A, Rezania D, Cualing H. Primary cutaneous B-cell lymphoma in a child. Fetal
Pediatr Pathol. 2008 Dec;27(4):206-214. Pubmedid: 18800263.
Cualing H,Moscinski LC, Zhong E. “Virtual Flow Cytometry” of Immunostained Lymphocytes on Microscopic Tissue
Slides, Clinical Cytometry part B, vol 72B, p 63-76, Jan 15, 2007.
Cualing H, Khalil F, Heekin J, Lili Miles.The rate and morphometric pattern of bone marrow recovery post-
myelosuppressive therapy for acute leukemia: a quantitative study-in press Archives of Pathology and Lab Medicine
2007
Yoshinori Takahashi, Domenico Coppola, Norimasa Matsushita, Hernani D. Cualing, Yuya Sato, James J. Mulé,
Hong-Gang Wang.Regulation of autophagy-dependent cell death and tumorigenesis by Bif-1. in press Nature Cell
Biology 2007.
Droc C*, Cualing H., Kadin M. The Need for an Improved Classification For Therapy of CD30 + lymphomas. Cancer
Control Journal, vol 14, no. 2, pp 124-132, April 2007.
Rezania D*, Cualing H. Aggressive Peripheral T cell Neoplasms and subtypes with high predilection in the Skin.
Cancer Control Journal, vol 14, no. 2, pp 112-123, April 2007
Rezania D*, Cualing H, Ayala E. Diagnosis, Management, And The Role Of Hematopoietic Stem Cell
Transplantation in Aggressive Peripheral T Cell Neoplasms Cancer Control Journal, vol 14, no. 2, pp 151-159, April
2007
Connie Keehn*, Cualing H., Marginal Zone Lymphoma with a Dual Cutaneous and Leukemic Presentation and an
Aberrant Myeloid Monocytic Phenotype. Journal of Cutaneous Pathology
Volume 33: 6, 236-241, March 2006.
Breneman DL, Raju US, Breneman JC, Steele PE, McFadden DW, Cualing HD, Nussbaum MS, Swerdlow SH.Reply
to Letters to the Editor: Lymph node grading for staging of mycosis fungoides may benefit from examination of
multiple excised lymph nodes. J Am Acad Dermatol. 2004 vol51:Sept 2004, 483-484.
Colvin JH, Lamerson CL, Cualing H, Mutasim DF. Cutaneous lymphoplasmacytoid lymphoma (immunocytoma) with
Waldenstrom's macroglobulinemia mimicking rosacea.
J Am Acad Dermatol. 2003 Dec;49(6):1159-62.
Breneman DL, Raju US, Breneman JC, Steele PE, McFadden DW, Cualing HD, Nussbaum MS, Swerdlow
SH.Lymph node grading for staging of mycosis fungoides may benefit from examination of multiple excised lymph
nodes. J Am Acad Dermatol. 2003 May;48(5):702-6.
Goldman A*, Bedolla G, Gebrail F, Cualing H, Bronchus-Associated Lymphoid Tissue Lymphoma (BALT
lymphoma). Vol 127, 115-116, Jan 2003, Archives of Pathol and Lab Med. (Direct supervision of medical student
elective in Diagnostic Hematopathology)
Kulow G, Breneman D, Cualing H, Steele P, Breneman J, Van Horn J, Mutasim D. The Progression of Cutaneous
B-cell Pseudolymphoma (CBPL) to Cutaneous B-cell lymphoma (CBCL). Vol 6(6), 519-528, Nov 2002, J of
Cutaneous Med and Surg.
Cualing H, Schroder L Perme I. Allergic Granulomatosis secondary to a Limited Churg-Strauss Lymphadenopathy:
Case Report with Histologic and Immunophenotypic analysis- 125:954-957, 2001 Arch of Pathol Lab Med July 2001
Cualing H. Perspectives: Automated Analysis in Flow Cytometry.Communications in Clin Cytometry, 42:110-113,
April 2000.*
Cualing H, Zellner D, Steele P. Blastic transformation of splenic marginal zone B-cell lymphoma.. Arch Pathol Lab
Medicine. 124(3):748-752 March 2000*
Marcelo A, Fontelo P, Farolan M, Cualing H. Effect of Image Compression on Telepathology: A Randomized
Controlled Trial. Arch Pathol Lab Med, 124:1653-1656, Nov 2000.
Cualing H, Balachander T, Kothari R. Immunophenotypic diagnosis of acute leukemia using decision tree analysis.
Laboratory Investigation , 79(2): 205-212, Feb 1999.*
Craig A*, Cualing H, Thomas G, Lamerson C, Smith R. Cytophagic Histiocytic Panniculitis- a syndrome
associated with benign and malignant panniculitis: Case comparison and review of the literature. J Amer Acad
Dermatol 39(5):721-736, part 1 Nov 1998.*
Massela F, Kowal-Vern A, Shrit A, Wibowo A, Rector J, Cotelingham J, Collier J, Michael A, Cualing H,
Schumacher H. Acute Erythroleukemia. Am J Clin Pathol 110(5):590-598, Nov 1998.
Cualing H, Vasireddy R. Adult T-cell leukemia and Cutaneous pathology: An overview. J Geriat Dermatol 5(8): 377-
382, 1997.*
Balachander T, Kothari R, Cualing H. An empirical comparison of dimensionality reduction techniques for Pattern
Classification. Gerstner W et al eds. Artificial Neural Networks ICANN ’97, Lausanne, Switzerland. Lecture Notes in
Computer Science1327: 589-594, 1997.
Cualing H, Povoski S, Fenoglio-Preiser C, Yassin R, Bell R. Characterization of Hematologic malignancies in Syrian
Golden Hamsters, Carcinogenesis 17(9):1983-1988,1996.*
Kothari R, Cualing H. , Balachander T. Neural Network Analysis of Flow Cytometric Immunophenotype
Data. IEEE Transactions in Biomedical Engineering, 43(8):803-810,Aug 1996.
Orazi A, Neiman R, Cualing H, Heerema N, John K. , CD34 Immunostaining of Bone marrow biopsy specimens is a
reliable way to classify the phases of chronic myeloid leukemia. Am J Clin Pathol, 101(4):426-428, Apr 1994.
Book Chapters:
Cualing H. Pathology of the Spleen. In Handbook of Hematologic Pathology, Schumacher H, Stass S. Eds. 53-73,
Marcel Dekker, Inc., New York, April 2000.
Book: Cualing Hernani, Editor: NonNeoplastic Hematopathology and Infections, Wiley Blackwell, NY, manuscript in
preparation
C. Past Research Support

Account #: MCC 13840 (Hernani Cualing MD)

Dates: 2005-2008
Source: Lymphoma Foundation of America
Title: A Phase II Trial Using a Universal GM-CSF-Producing and CD40L-Expressing Bystander Cell Line
(GM.CD40L) in the Formulation of Autologous Tumor Cell-Based Vaccines for Patients with Mantle Cell Lymphoma.
% Effort:2 % Goal: Translational investigation of the clinical and histopathologic factors associated with responders:
Role in the Study: Hematopathologist-Tissue Quantification of tumor infiltrating lymphocytes in mantle cell
lymphoma.
Clinical Trial 2006-Medarex ALCL phase I trial using Anti-CD30, Moffitt Cancer Center, Coinvestigator, 2 % effort.
2005-2007 Translational study on immunomodulating monoclonal antibody treatment for peripheral T cell
lymphomas. Role: Hematopathologist and quantification of target antigen
Clinical Trial 2006-Medimmune anti CD2, Coinvestigator, 2 % effort. Moffitt Cancer Center,2006-2007Translational
study on immunomodulating monoclonal antibody treatment for peripheral T cell lymphomas. Role:
Hematopathologist quantifying target antigen.

2007 Division of Hematopathology Internal Research Funding for T regulatory tumor infiltrating lymphocytes in
lymphomas. Role: Principal investigator 5 % effort.
PENDING
Application PI/George Blanck, Ph.D. Co Investigator/Hernani Cualing, MD
Source: National Institute of Health
Title:“Mouse modeling of a CD30+ lymphoproliferative disorder: Role of IRF-1 defects in ALCLs”
% Effort 10%
Role in Study: Co PI: Hematopathologist comparing human CD30+ lymphomas with mouse CD30+ lymphomas
New Multiinstitutional Grant 9: Exploratory/Developmental Research Grant (R21) via American Recovery &
Reinvestment Act of 2009 as RFA to Translational Science and specific Challenge Topic 15-CA-105, The Biology of
Cancer in Adolescents and Young Adults.: using tissue blocks from SEER tissue banks from Hawaii, Iowa, RI, USC
Samples consist of diagnostic tumors from 527 Hodgkin lymphoma patients in the National Cancer Institute
Surveillance, Epidemiology and End-Results (SEER) program Residual Tissue Repository (RTR). In Los Angeles,
Hawaii and Iowa.
% effort: 3 %
Role: To provide hematopathology support and know-how for automated virtual flow analysis of T cell subsets
immunostains on these blocks.

PATENTS and TRADEMARKS

1. USPTO/Virtual flow cytometry on immunostained tissue-Tissue cytometer -awarded
2. USPTO/ International PCT :Automated Cytomics: Bone Marrow Cellularity Determination-pending
3. USPTO/ Automated Cytomics: Bone Marrow Reticulin Fibrosis Determination
4. USPTO Registered Trademark: VirtualFlow IHCFLOW®
D. Teaching and Lectures
April 28, 2007 Co-director of 1st Annual Cutaneous Lymphoma Seminar, Moffitt Cancer Center, Tampa,
Sponsored by Moffitt Cancer Center Foundation and jointly with Cutaneous Lymphoma
Foundation- Update on Skin Lymphomas
April 19, 20 2008 Director of 2nd Annual Cutaneous Lymphoma Seminar,
Sponsored by Cutaneous Lymphoma Foundation, USF Health, Moffitt Cancer Institute
and Funded by Eisai Corporation, and other National Pharmaceutical Corporations
Lake Buena Vista Resort, Orlando Florida
This two day seminar included three patients as speakers and Faculty from International Cutaneous
Lymphoma Study Group, Harvard Medical School Faculty and attended nationally by Dermatologist,
Pathologists, Oncologists and some attendees from out of the country.
Teaching Series

1992-2010 Hematopathology Lecture Series for Residents and Fellows

Hematopathology Fellow Teaching -Slide Weekly Slide Workshop Series
Hematopathology Fellow Teaching -Slide Weekly Slide Workshop Series