Professional Documents
Culture Documents
Biochem Answers To Q14 30
Biochem Answers To Q14 30
28.
Eadie-Hofstee plot:
𝑉 𝑉 𝑉max
=− +
[S] 𝐾𝑀 𝐾𝑀
1
𝑉𝑚𝑎𝑥 = (649.806 s −1 )𝐾𝑀 = (649.806 s −1 ) ( ) = 65.0 mM/s
9.996 mM −1
29.
Hanes-Woolf plot:
[S] [S] 𝐾𝑀
= +
𝑉 𝑉max 𝑉max
1
𝐾𝑀 = (1.5389 × 10−3 s)𝑉max = (1.5389 × 10−3 s) ( ) = 0.100 mM
0.0154 s/mM
30.
Lineweaver-Burke plot:
1 𝐾𝑀 1 1
= +
𝑉 𝑉max [S] 𝑉max
1 1 1
= (1.5384 × 10−3 s) + 0.0154 s/mM = (1.5384 × 10−3 s) ( ) + 0.0154 s/mM = 0.02155 s/mM
𝑉 [S] 0.25 mM
1
𝑉= = 46.4 mM/s
0.02155 s/mM