Elsevier/2017/Journal of Biotechnology /Application of next generation

sequencing in clinical microbiology and infection prevention:-

Q1. Where this research is useful?
Ans. The medical microbiology diagnostic laboratory.
The field of research is useful for identification and characteristics of microorganism that cause
infection in human being. The present is useful successful for treatment
Q2. What was lacking in the present state of knowledge in this field or author felt he should
improve?
Ans. Current molecular diagnostics of human pathogens provide limited information that is often
not sufficient for outbreak and transmission investigation.
Molecular diagnostic method is used for identify the microorganism. Sequence analysis is used
to answer diagnostic question such as genetic relationship, detection of mutuation, viral or
bacterial genomes etc.
The sequential have got limitation & their reliability is often doubtful. The cost of present
sequencing method is also high & turnaround time is long.
Q3. What was author contribution to fill up this technological gap?
Ans. Author has introduced a new technology, named, Next generation sequencing (NGS) which
determines the DNA sequence of a complete bacterial genome in a single sequence run, and from
these data, information on resistance and virulence, as well as information for typing is obtained,
useful for outbreak investigation. The obtained genome data can be further used for the
development of an outbreak-specific screening test.
The Next Generation Sequencing (NGS) which is used for detection lacks the capability of in
depth analysis which can generate genomic feature & microbiological background of
microorganism under investigation.
This NGS data from sample needs to be improved to detect the resistance genes and application
of something (biological etc.)
Q4. Read the conclusion and comment if author has succeed.
Ans. For generating NGS data from samples originating from humans, animals, food and the
environment, the same laboratory protocol for library preparation can be used, and, after data
analyses, information on the presence of specific antibiotic resistance and virulence genes is
obtained. Furthermore, NGS makes it possible to standardise typing methods for pathogens (“one
test fits all”).