In conclusion, the group was able to compute the number of cells using the hemocytometer.

The

1.) If you have a total of 170 cells in all 4 corner squares, what is the cell count (cells/mL)?
𝑇𝑜𝑡𝑎𝑙 𝑛𝑢𝑚𝑏𝑒𝑟 𝑜𝑓 𝑐𝑒𝑙𝑙𝑠 170
= = 𝟒𝟐. 𝟓 𝒐𝒓 𝟒𝟑 𝒄𝒆𝒍𝒍𝒔 𝒑𝒆𝒓 𝒔𝒒𝒖𝒂𝒓𝒆
𝑁𝑢𝑚𝑏𝑒𝑟 𝑜𝑓 𝑐𝑜𝑟𝑛𝑒𝑟𝑠 4
𝑐𝑒𝑙𝑙𝑠
= 𝑎𝑣𝑒𝑟𝑎𝑔𝑒 𝑐𝑜𝑢𝑛𝑡 ∗ 𝑑𝑖𝑙𝑢𝑡𝑖𝑜𝑛 𝑓𝑎𝑐𝑡𝑜𝑟 ∗ 104 = 43 ∗ 3 ∗ 104 = 𝟏. 𝟐𝟗 ∗ 𝟏𝟎𝟔 𝒄𝒆𝒍𝒍𝒔/𝒎𝑳
𝑚𝐿

2.) What is the significance of the value (104 ) in equation 4-2?

Each corner square has a surface area of 1 mm2 and a depth of 0.1 mm, which would result to a volume
of 10-4 cm3 or 1 mm3. Each large square has a volume of 0.0001 ml. Multiplying the average number of
cells by 104 will give the count of cells per ml which are the actual values outside the counting chamber.

3.) Why do you have to count the nonviable cells?

It is important to count nonviable cells because it is used for computing the percent viability. The
percent viability represents cell viability which shows the number of living cells in relation to non-living
cells or nonviable cells. Low percent viability presents that cells are in less optimal conditions while high
percent viability means that cells are in proliferative state.