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Exam1 2005 Blank PDF
Exam1 2005 Blank PDF
Name
Make sure that your name is on every page. This is the only way we have of
matching you with your exam after grading it. Please work independently.
Read each question carefully before answering. Unless otherwise indicated,
there is only one correct answer for each multiple choice question. Points are
indicated by the question within brackets [].
(A) D-isoleucine
(B) L-isoleucine
(C) L-alloisoleucine
2. [1] Which one(s) will rotate the plane of polarized light to the same extent but
in opposite directions? _________
(A) DDDAAGKAED
(B) LIAAVALLA
(C) WGGKFYARAGED
4. [1] Which one will absorb the most ultraviolet light? _________
7. [2] Lysine has pKa1 = 2.18, pKa2 = 8.95, and pKaR = 10.53. What is the isoelectric
8. [2] A solution of an amino acid that has a light absorbance of 2.0 at 280 nm
____%.
Name 2
9. [2] In the Edman degradation of proteins, the amino acid that is removed
from the protein at each cycle comes from the ___________ end? (indicate
amino or carboxyl).
10. [2] Which one phrase best describes the hydrogen bonds in a β sheet?
a) they occur mainly near the amino and carboxyl termini of the strands.
d) they occur only between some of the amino acids of each strand.
11. [2] The structure of an α helix is stabilized by (one or more may be correct):
13. [4] Fill in the missing words: In the protein, __________________, additional
strength is provided by the post-translational ____________________ of
some amino acids to increase the number of __________________ bonds
they can form. This modification becomes deficient when
____________________ is lacking in our diet.
15. [2] A mutant myoglobin has a Ka for O2 of 0.25 kPa–1. What is the value of
θ when the partial pressure of O2 is 0.125 kPa? ______________
16. [1] The proximal histidine residue in myoglobin acts to (choose one):
d) prevent binding of N2
e) decrease θ
17. [3] For myoglobin, when θ = 0.25, what is the value of [PL]/[P]? ______.
Name 4
Supply the missing word(s) in each of the following 4 questions. Choose from
increase, decrease, or remain unchanged.
19. [2] Decreasing the pH of a solution of hemoglobin causes the affinity for O2
to ___________.
21. [2] Adding BPG to a solution of adult hemoglobin causes the affinity for O2
to ___________.
22. [2] Enzymes catalyze the rapid formation of product by (choose one):
23. [3] Two samples of the same enzyme were purified by different students in
an undergraduate laboratory. The students determined that the two
samples had identical Vmax values, but very different values of Km. Which
one of the following explanations for these facts is most likely?
a) One sample inadvertently contained a noncompetitive inhibitor.
b) One sample inadvertently contained a mixed inhibitor.
c) The samples had different affinities for the substrate.
d) The students had used very different substrate concentrations.
e) The students had used very different enzyme concentrations.
Name 5
24. [4] A series of initial velocity measurements for an enzyme revealed that
the substrate concentration required for Vo to be one-fourth of Vmax
was 3.0 mM. What is the Km for this enzyme? ______ mM
25. [4] For an enzyme that has a Km = 5 mM, what is the substrate concentration
required for Vo to be one third of Vmax? _________ mM
26. [4] If the enzyme described above has a Vmax of 3 µmol/min at an enzyme
concentration of 4 µg/ml, what would be the enzyme concentration
required for a Vmax of 9 µmol/min? ___________ µg/ml
27. [3] In the following oligopeptide, indicate by drawing vertical line(s) where
chymotrypsin would hydrolyze peptide bond(s):
D-A-V-T-W-C-L-A-F-T-K-S-G-S-Y-H-I-L-R
28. [2] Which of the following is/are true for a competitive inhibitor? (There
may be more than one.)
a) it lowers kcat
b) it lowers the apparent Km
c) it raises the substrate concentration required for the rate to equal
Vmax
d) it raises the substrate concentration required for the rate to be 1/2 of
Vmax
e) it lowers the substrate concentration required for the rate to be 1/2
of Vmax
33. [2] If the ΔG of the reaction A → B is –30 kJ/mol, which of the following
statements is/are correct? (There may be more than one.)
a) The reaction would proceed spontaneously from left to right at
standard conditions.
b) The equilibrium constant could be calculated if the concentrations of A
and B and the temperature were known.
c) The reaction would proceed spontaneously from left to right at the
given conditions.
d) The value of ΔG´o is negative.
e) The equilibrium constant favors the formation of B over the formation
of A.
34. [6] For each class of enzyme inhibitor listed below, indicate the effect on the
Vmax and apparent Km of the enzyme. Choose from raise, lower, or no effect.
35. [2] A Lineweaver-Burk douple reciprocal plot in which all of the data points
fall very close to the straight line implies (choose one):
36. [2] Which of the following feature(s) do a micelle and a lipid bilayer have in
common? (There may be more than one.)
a) Both are made up of amphipathic molecules.
b) Both are very large, sheetlike structures.
c) Both are stabilized by electrostatic interactions.
d) Both consist of molecules having two long-chain hydrocarbons.
e) Both require proteins for stable assembly.
Name 8
37. [3] Indicate the effect on the membrane fluid transition temperature of the
following changes to the fatty acids in the membrane lipids (choose
raise, lower, or no change):
a) Increase the average chain length of fatty acids ______________
b) Increase the average number of double bonds in the fatty acids _________
c) Change some cis double bonds to trans double bonds _______________
What is ΔG´o for the overall reaction fructose 6-P → glucose 1-P?
a) 12.4 kJ/mol
b) –12.4 kJ/mol
c) 5.6 kJ/mol
d) –5.6 kJ/mol
e) 9.0 kJ/mol
f) –9.0 kJ/mol
39. [3] If the reaction glucose 1-P → glucose 6-P has ΔG´o = –7.3 kJ/mol, the ΔG
for the reaction when the concentration of glucose 6-P is ten times that of
glucose 1-P will be
c) –7.3 kJ/mol
Name 9
40. [2] Which pair or pairs of the above molecules are epimeric? _____________
41. [2] Which pair or pairs of the above molecules are enantiomeric? ________
42. [2] Which of the above molecules contain (a) pyranoside(s)? ––––––––
43. [2] Which of the above molecules is/are (a) reducing sugar(s)? ––––––––
44. [2] Which of the above molecules contain(s) at least one α anomer? ––––––––