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Melanic Moth Frequencies in Yorkshire, An Old English Industrial Hot Spot
Melanic Moth Frequencies in Yorkshire, An Old English Industrial Hot Spot
Abstract
A survey has been carried out in Leeds, England, in the west Yorkshire industrial heartland, and in neighboring York,
surrounded by agriculture, of melanic frequency in the moth species Biston betularia, Odontoptera bidentata, and Apamea crenata.
All show a decline in melanics in the postindustrial environment, the first over almost the full range from nearly 100% to
less that 10%, the others to smaller extents. Changes in several species over as great a magnitude and as wide an area must
result from selection. The results are compared with others along a transect through northern England. The onset of
response is progressively later from west to east. The rate of decline is lower at the extremes of the transect to west and east
than it is in the center. We still do not have a clear picture of the causes of the changes. One major factor is likely to be
selective predation, which is shown to be critically dependent on predation rate. As a consequence, differences in settling
behavior between the species could account for different responses even if the species are attacked by the same predators.
It is well known that melanic forms in numerous moth From the middle of the 20th century, there has been
species increased in frequency in parts of Britain following a general environmental improvement and a corresponding
industrialization in the 18th and 19th centuries (Kettlewell reduction in melanic frequencies (Clarke et al. 1985, 1994;
1965; Kettlewell 1973; Majerus 1998). Northern England, Cook 2003). Similar changes have been seen in the United
with its intensive coal-fired manufacturing industry, was States (Grant and Wiseman 2002). West Yorkshire, with
a center both of environmental change and of the earliest Leeds and it sister city, Bradford, at its center, was one of the
records of response by moth populations (Cook 2003; major British industrial regions and one of the last to
Kettlewell 1973; Lees 1981). Blackening of trees, buildings, experience a decline in morph frequencies (Cook et al. 2002).
and vegetation was extreme, and the atmosphere was heavily York is situated in an extensive rural region 40 km to the
polluted with a variety of toxic gases. The response of moth northeast. Comparatively little information is available from
populations in west Yorkshire was noteworthy enough for Yorkshire, compared with industrial Lancashire and rural
special comment when the British Association for the north Wales, which lie to the west separated from Yorkshire
Advancement of Science met in York in 1906 (Porritt 1907). by the Pennine chain of mountains. To enlarge the area
The earliest indication of an effect was the appearance of examined a study was set up 37 years ago by S. L. Sutton in
extreme black forms in the peppered moth Biston betularia (L.). Leeds to monitor the change in frequencies in the best
They attracted attention during the 1870s and had become known example, B. betularia, and for comparison, O. bidentata
the prevalent form by the start of the 20th century. In and A. crenata. The first two species are members of the
another species, the scalloped hazel Odontoptera bidentata subfamily Ennomiinae of the Geometridae. A. crenata
(Clerck), melanics began to be seen in the 1890s and within belongs to the subfamily Amphipyrinae of the Noctuidae.
a decade had become very common. Porritt lists 30 species in All have annual nonoverlapping generations and fly from
which such distinct black forms were regularly obtained. Of early May to June (O. bidentata) or May to July for the other
these, seven had been recorded before industrialization, two species.
whereas others were novel. He added another 21 species in A set of results for the same species were collected by
which dark individuals occurred so frequently as to suggest T. J. Crawford from 1990 to 2004 near York. The new data
they were part of the same phenomenon, among them the extend records eastward, so that we now have a transect over
clouded bordered brindle, known then as Xylophasia rurea and 200 km in extent passing through a full range of available
now as Apamea crenata (Hufnagel). habitats and climate types in north Wales and northern
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Cook et al. Melanic Moths in Yorkshire
Figure 1. Sketch map of northern England and Wales showing the approximate locations of the centers mentioned in the text.
C: Caldy, Wirral peninsula, Li: Liverpool, M: Manchester, Le: Leeds, Y: York. The 100 km squares of the National Grid are shown.
England. The main locations referred to are shown in Figure 1. nigra. The genetics of melanic mutants in both species was
The efforts of recorders at the beginning of the 20th century first established by Bowater (1914). A. crenata was scored as
were of immense importance in bringing the phenomenon of dark or pale. The two types are very distinct. The pale form is
industrial melanism to the attention of the scientific straw-colored with mahogany brown markings at the edges
community. The records were nevertheless often fragmen- of the wings, whereas the dark form has the entire forewing
tary and anecdotal. We hope to ensure that more detailed mahogany-colored except for a paler line round the orbicular
material is available for analysis from the end of the period of and reniform stigmata. Kettlewell (1973) used the name
high melanic frequency. alopecurus for this form. The correct name is probably combusta
Howarth, which is used by South (1948), Skinner (1984),
Sutton and Beaumont (1989), and other more recent authors,
Materials and Methods whereas alepecurus Esper refers to a darker form with black
costal streaks and edge to the reniform stigma (Bretherton
The Leeds survey was carried out from 1967 to 2003 at sites et al. 1983). The dark insects at the sampling sites all have
10 km NE of the city center in an area of suburban building the same phenotype. The three species are illustrated by
and farm land. For the first 3 years the site was at Scholes Kettlewell (1973), Bishop and Cook (1980), and Majerus
(National Grid Reference 4377 4366) and subsequently 4 km (1998). All collecting and identification at Leeds was made by
distant at Bond Ing meadow, Shadwell (GR 4344 4394), or under the supervision of S. L. Sutton and at York by
a mature garden and pasture with extensive planting of trees T. J. Crawford.
and hedges. Sampling was by mercury vapor light trap and All analysis is based on comparison of melanic and
took place throughout the flying season where possible, but nonmelanic forms, without reference to genotype (the
sometimes for shorter periods. This method of trapping melanic forms in the two geometrids are dominant, and we
collects almost entirely males in the species concerned, but have no information on A. crenata). The selective disadvan-
the variation is the same in the two sexes. The York site is at tage v of the melanic form relative to the nonmelanic has
Haxby (GR 4610 4587), about 5 km north of the city center been estimated from the equation L0 : M0(1 v)n ¼ Ln : Mn
on the edge of the built-up area. The same type of light trap where the L and M are the initial frequencies and the final
was used, and continuous sampling was carried out during frequencies after n generations of nonmelanics and melanics.
the relevant part of every year. It follows from this relation that when the logarithms of the
B. betularia was scored as the dominant melanic carbonaria ratios show a linear change with generation the slope
Jordan or the dotted white typical. At Leeds the intermediate provides an estimate of v. The calculation has been found to
insularia forms have been extremely rare. Only about 10 give an adequate degree of accuracy and to be robust under
specimens were seen throughout the sampling period, so this conditions of high or low frequency (Cook et al. 1999a).
type was ignored. At York, insularia was more common and
has been recorded (see Cook and Muggleton, 2003, for
a summary of information on the melanic types). O. bidentata
was scored as the dominant melanic nigra Prout or as typical.
Results
Typicals from this region are darker than those from The numbers collected at Leeds in the three species are
southern England but always clearly distinguishable from shown in Table 1. There was a gap in collecting in 1988–89,
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Journal of Heredity 2005:96(5)
Table 1. Numbers of moths in three species caught in Leeds Table 2. Numbers of moths in three species caught in York
(melanics are shown in the first column for each species,
nonmelanics in the second) Date B. betularia O. bidentata A. crenata
1990 11 2 6 2 5 — —
Date B. betularia O. bidentata A. crenata
1991 21 1 12 1 4 3 26
1967 47 0 34 10 — — 1992 20 0 7 2 4 3 10
1968 58 0 44 19 — — 1993 16 1 6 3 7 3 11
1969 27 0 32 19 — — 1994 9 0 6 1 6 3 8
1970 75 1 10 20 32 12 1995 6 0 2 4 20 5 12
1971 41 0 10 5 40 6 1996 19 3 15 6 12 2 5
1972 76 0 22 6 26 6 1997 8 2 9 5 15 1 5
1973 40 1 40 20 72 31 1998 7 2 8 3 19 0 6
1974 40 0 24 16 25 10 1999 10 0 17 7 20 3 4
1975 3 0 28 7 33 10 2000 5 3 16 3 11 1 4
1976 19 2 23 21 34 13 2001 7 2 12 4 14 4 8
1977 18 0 12 12 4 2 2002 14 0 30 3 23 2 6
1978 23 2 75 33 26 15 2003 7 1 19 3 11 6 10
1979 43 3 48 35 15 4 2004 2 1 12 2 9 1 3
1980 49 2 64 62 74 37
1981 — — — — — — Note: The columns for B. betularia are carbonaria, insularia, and typical. For the
1982 48 4 53 4 77 33 other two, melanics in first column, nonmelanics in second.
1983 57 4 10 9 97 40
1984 27 1 21 18 69 24
1985 48 5 17 12 56 24
1986 83 3 23 13 94 58 Discussion
1987 12 1 14 13 69 32
1988 — — — — — —
B. betularia
1989 — — — — — — In Figure 2 a curve has been fitted by eye to the Leeds data to
1990 5 2 2 3 51 31 indicate the observed trend in frequency of carbonaria. From
1991 34 16 3 18 75 37
1992 27 9 16 20 96 46
a situation where there were almost 100% melanics in the late
1993 18 11 11 7 19 21 1960s, the frequency has declined to less than 10%. The
1994 7 11 4 2 19 20 territory extending WSW from Leeds across Lancashire and
1995 1 5 3 4 8 13 Cheshire and into north Wales has received considerable
1996 4 1 5 7 3 3 attention in studies of morph frequency (Bishop, 1972;
1997 2 1 0 0 0 0
1998 1 8 1 3 5 2
1999 9 13 10 10 8 7
2000 6 18 11 18 33 19
2001 — — — — — —
2002 — — — — — —
2003 1 15 8 16 41 28
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Cook et al. Melanic Moths in Yorkshire
Figure 3. Change in frequency of the nigra melanic form of Figure 4. Change in frequency of the dark form of the
the scalloped hazel moth O. bidentata in Leeds and York. Data clouded-bordered brindle moth A. crenata in Leeds. Vertical
are means of pairs of years, indicated by horizontal lines. lines are standard errors, horizontal line shows where data have
Vertical lines are standard errors. been grouped.
Bishop et al. 1978; Clarke and Sheppard 1966; Cook 2003). other processes is likely to be negligible in comparison. Over
There are also temporal series of records for Manchester and the period 1980–2003 the disadvantage of the melanic
Caldy. Manchester is separated from Leeds by the Pennines morph compared with typical (estimated from the slope of
but is within the region that had over 95% carbonaria during log morph ratio on year for the period when the slope was
the 1950–70 period (Cook et al. 1999b). Caldy is on the linear) was 18.9 6 2.6% for York, 27.9 6 2.1% for Leeds,
Wirral peninsula west of Liverpool (Clarke et al. 1994; Grant 29.2 6 3.5% for Manchester, and 22.3 6 0.8% for Caldy.
et al. 1996). In 1959 it had 93% carbonaria, but the location is Selection was most intense where initial frequency was
just to east of the cline in north Wales, which saw the higher (for the comparison of Leeds with Caldy the differ-
frequency drop to 5% over about 25 km. It is the most ence is significant, p , .05).
complete data set, being based on a series of large samples When initial frequencies are very high, however, the
collected annually until 2002. Manchester is about 65 km balance of the different forces changes, and makes
from Leeds, and Caldy about 130 km. The data for both sites estimation difficult. If carbonaria gene frequency in Leeds
are plotted together for comparison in Cook (2003). was truly 100% before 1970, then no change would have
Curves M and C in Figure 2 are lines similar to that for been possible without introduction of typicals by mutation
Leeds, fitted to the data for Manchester and Caldy, or migration of immigrants from regions of lower frequency.
respectively. It can be seen that as one proceeds west the Because typical is recessive, these processes would have
initial frequency becomes lower, the decline commences introduced genes in heterozygotes that were not subject to
earlier and is less steep. At Leeds the drop to 90% carbonaria selection, and the response to selection would necessarily be
occurred nearly 20 years after that at Caldy. It reached 50% very slow. It is likely that the response observed was in some
9 years later than at Caldy and 6 years after Manchester. The part due to immigration. Population density is probably
data for York are shown inserted on Figure 2, using the same lower in industrial than in rural areas (Cook 2003), so that to
grid of frequency and dates and with the Leeds curve the west of the Pennines, net migration is likely to be
superimposed as a comparative guide. The commencement eastward, bringing with it the typicals characteristic of north
of the decline is as delayed at York as at Leeds. Figures from Wales. We do not know whether there are areas of lower
the two places for the early 1990s map onto each other. frequency farther east than York from which immigration
Thereafter the drop has been slower at York, and the may take place. For two points nearer the east coast
frequency of carbonaria is probably still appreciably higher Beaumont (2002) notes that at Spurn (; GR 5400 4100)
than at Leeds. As noted long ago by Kettlewell (1965, 1973), there was recently a rapid change from the majority being
the effects of industrialization on melanic frequency appear melanic to the majority being typical, while at Hutton Rudby
to be displaced to the northeast. (GR 4507 5506) there has been a steady decrease in melanics
The changes observed result from all the processes from about 70% in 1990 to about 40% in 2000. Selection
operating on the populations, namely selection, migration probably also changes with time. The data for Caldy cannot
from populations with different frequencies, mutation, and be fitted by a constant selection curve (Grant et al. 1996),
random drift. Changes of as great a magnitude over as wide selection apparently being low at the start, greater during the
an area as observed must involve selection, and over the main period of change, and probably finally falling off. Such
middle part of the frequency range the contribution of the variation in selection may well occur at the other sites.
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Journal of Heredity 2005:96(5)
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Cook et al. Melanic Moths in Yorkshire
enclosures with both exposed surfaces and features that especially when there are high or low frequencies. Predation
allowed the moths to hide. There was a very strong tendency rate must be substantial to ensure that predator choice is
for O. bidentata to find covered or protected sites whereas translated into change in the prey population, but this will
B. betularia remained exposed (v2 ¼123.7 and 285.7, d.f. ¼ 1, put the continued survival of the population at risk.
respectively, in two trials). Like many other noctuids, Predation rate in the wild is very difficult to measure now,
A. crenata spends the day near the ground in long grass or and it is impossible to know what it was under the conditions
herbage (Bretherton et al. 1983). Apart from differences in that brought about industrial melanic patterns; it may have
appearance that will make them differently visible, these been higher in industrial regions than in rural ones because
species are likely to experience different levels of predation fewer inconspicuous resting sites were available. Different
even within the same location. It is therefore interesting to exposure to predation and different average densities may
consider the possible effect of intensity of predation, as generate widely different results in species subject to the
distinct from predator discrimination. same predators. It is therefore to be expected that response
will differ even if selective predation is the common factor
involved. New experimental information would help us
Consequences of Selective Predation understand the interactions, but practical problems of
translating results into reliable evidence of causation remain.
Suppose there are N individuals in a predation experiment or
natural predated population, of which L are typical and M are
melanic (L þ M ¼ 1). In the absence of selective removal Acknowledgments
a fraction I is eaten (0 , I , 1). Predators detect the two
forms differently. Suppose that for every typical detected 1 a We thank Pam, Norman, and David Taylor, Dan Houldsworth, and Sam
Rose for help with collecting in Leeds and John Muggleton for comments on
melanics are detected. Choice by predators is exerted
the draft. Information on atmospheric pollution in Bradford is from
through prey individuals eaten, while the effect on the prey www.bradford.gov.uk.
population is measured on those left. This can be expressed
as a coefficient v, such that
L : ð1 vÞM ¼ Lð1 I Þ : M ½1 I ð1 aÞ: References
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