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861 Coincident Hepatitis B Surface Antigen and Antibodies of Different Subtypes in Human Serum
861 Coincident Hepatitis B Surface Antigen and Antibodies of Different Subtypes in Human Serum
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Coincident Hepatitis B Surface Antigen and Antibodies of Different
Subtypes in Human Serum
E D W A R D T A B O R , 1 R O B E R T J. G E R E T Y , L I N D A A. S M A L L W O O D , AND L E W E L L Y S F. B A R K E R
From the Division of Blood and Blood Products, Bureau of Biologics, Food and Drug Administration, Bethesda, Maryland
Evidence from a number of sources indicates a protective purified HBsAG, subtypes adw and ayw, followed by testing
role of antibodies to hepatitis B surface antigen (anti-HBs) ~in the adsorbed sera for anti-HB~ in the solid-phase RIA test for
resistance to infection with hepatitis B virus (HBV) (1-3). anti-HBs (14). Anti.HB~ specificity was also tested on serum
TABLE I were studied for subtypes of HB~Ag and anti-HBs. In each case
Subtyping o[ HB,Ag and anti-HB, by RIA anti-HB.~ was found to be directed to a different subtype than
HB,AgSubtyping HB,AgSubtyping Anti-HB~Subtyping that of the circulating HBsAg, indicating that reinfection (or
(Direct)" (Indirect ~~ (Indirect)~ simultaneous infection) with a second subtype occurred.
Anti-d Anti-y
after after Sample
Serum Sample Sample adsorption adsorption Sample after REFERENCES
No. on anti-d on anti-y with with after adsorp-
coated c o a t e d s a m p l e s a m p l e adsorption tion
beads beads (9.02 (20.00 with adw with 1. Hoofnagle, J. H., R. J. Gerety, and L. F. Barker. 1975. Antibody to
before before ayw hepatitis B core antigen. In Transmissible Disease and Blood
adsorption} adsorptionl
Trans[usion. Edited by T. J. Greenwalt, and G. A. Jamieson.
1 36.01 0.27 8.45 ¢ 16.35 c 14.54 1.32 Grune & Stratton, New York. P. 43.
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control cpm. 3. Krugman, S., and J. P. Giles. 1973. Viral hepatitis, type B
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in RIA ratio after adsorption using subtype specific reagents. tion. N. Engl. J. Med. 288:755.
c Sera Nos. 1 and 2 did not inhibit either anti-d or anti-y on indirect 4. Markenson, J. A., R. J. Gerety, J. H. Hoofnagle, and L. F. Barker.
assay. 1975. Effects of cyclophosphamide on hepatitis B virus infection