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  • Data Pengamatan Praktikum Biokimia P1. Penetapan Asam Amino Dengan Titrasi Formol

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    Dyah Aji Sofyaningtyas
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    This document outlines the procedure for determining the amino acid content of a sample through titration with formaldehyde. It involves weighing samples, calculating the normality of NaOH, performing titrations in replicates at various time points, and calculating the percentage of amino acids based on the titration values. The percentages of amino acids will be calculated for each time point in the two replicates to observe any changes over time.

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    Data Pengamatan

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    This document outlines the procedure for determining the amino acid content of a sample through titration with formaldehyde. It involves weighing samples, calculating the normality of NaOH, performing titrations in replicates at various time points, and calculating the percentage of amino acids based on the titration values. The percentages of amino acids will be calculated for each time point in the two replicates to observe any changes over time.

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    © All Rights Reserved
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    12 views4 pages

    Data Pengamatan Praktikum Biokimia P1. Penetapan Asam Amino Dengan Titrasi Formol

    Uploaded by

    Dyah Aji Sofyaningtyas
    This document outlines the procedure for determining the amino acid content of a sample through titration with formaldehyde. It involves weighing samples, calculating the normality of NaOH, performing titrations in replicates at various time points, and calculating the percentage of amino acids based on the titration values. The percentages of amino acids will be calculated for each time point in the two replicates to observe any changes over time.

    Copyright:

    © All Rights Reserved
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    of 4

    DATA PENGAMATAN

    PRAKTIKUM BIOKIMIA

    P1. PENETAPAN ASAM AMINO DENGAN TITRASI FORMOL

    1. Penimbangan Bahan
    Nama Bahan Kaca Kosong Kaca + NaOH Kaca + Sisa Berat NaOH
    NaOH

    Nama Bahan Kertas Kosong Kertas + Bahan Kertas + Sisa Berat Bahan
    Papain
    Gelatin

    2. Perhitungan Normalitas NaOH sesungguhnya


    gram 1000
    N= × × Valensi
    Mr P
    1000
    N= × ×
    N=

    3. Tabel Titrasi Formol


    Menit Replikasi 1 Replikasi 2
    ke- Vol. NaOH % N Asam Amino Vol. NaOH % N Asam Amino
    0
    15
    30
    60
    90
    120
    4. Perhitungan % N Asam Amino
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    a) Replikasi 1
    Menit ke-0
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-15
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-30
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-60
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-90
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %
    Menit ke-120
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    b) Replikasi 2
    Menit ke-0
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-15
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-30
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-60
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %
    Menit ke-90
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

    Menit ke-120
    Vol. NaOH x N NaOH x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    x x 1,4
    % N Asam Amino = x 100%
    0,5 x 10
    % N Asam Amino = %

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