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Jurnal Teknologi Dan Industri Pertanian Indonesia: Keywords: Lactobacillus Delbrueckii
Jurnal Teknologi Dan Industri Pertanian Indonesia: Keywords: Lactobacillus Delbrueckii
5903
http://Jurnal.Unsyiah.ac.id/TIPI
PRODUKSI ASAM LAKTAT OLEH Lactobacillus delbrueckii subsp. bulgaricus DENGAN SUMBER
KARBON TETES TEBU
INFO ARTIKEL
ABSTRACT
Submit: 20 Desember 2016
Lactic acid is needed in industrial bioplastics. However, the microbial production still uses food
Perbaikan: 5 Februari 2017
Diterima: 15 Februari 2017 material for the substrate. Alternative substrates for the production of lactic acid are needed in
industry. Molasses are potential substrates due to the richness in carbon. Molasses are also widely
available and low-cost material. The objective of the research was molasses can be used as a carbon
Keywords:
source needed by Lactobacillus delbrueckii subsp. bulgaricus to produce lactic acid. This study
fermentation, lactic acid, consisted of hydrolysis and detoxification of molasses, analysis qualitative test of reducing sugar
Lactobacillus delbrueckii
from molasses, analysis of total sugar by phenol sulfuric acid, determination of bacterial growth,
subsp. bulgaricus, molasses
production and extraction of lactic acid, and analysis of lactic acid using high performance liquid
chromatography (HPLC). The results showed that molasses can be used as an alternative carbon
source as indicated by the growth of bacteria when the media were given 0,5% molasses.
Concentration of the total sugar molasses was 1090 g/L. The reducing sugar test showed positive
results for the Selliwanoff, Benedict, and Barfoed tests. The optimum of L. delbrueckii subsp.
bulgaricus growth was at temperature of 42° C and 150 rpm of agitation. Production of lactic acid
was conducted in 24 hours. The lactic acid content was 2,80%. The dry cell biomass was 0,002 g/L
at pH of fermentation media 4,0. Analysis HPLC also showed that lactic acid was the product of
fermentation.
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selama 24 jam, biomassa sel kering yang bioplastics 2014 (by material type).[terhubung berkala]