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SILAC-based Proteomics Analysis
SILAC-based Proteomics Analysis
SILAC Workflow:
SILAC relies on metabolic incorporation of a given 'light' or 'heavy' form of the amino acid into
two samples. As the two isotopically labeled amino acids are essentially chemically identical,
their incorporation does not interfere with normal cell growth, while leading to
proteins/peptides that are distinguishable by mass and thus are ideal for mass spectrometric
analysis. The method relies on the incorporation of amino acids with substituted stable
isotopic nuclei. Thus in an experiment, two cell populations are grown in culture media that
are identical except that one of them contains a 'light' and the other a 'heavy' form of a
particular amino acid (e. g. 12C and 13C labeled L-lysine, respectively). The SILAC samples
are then subjected to enzymatic digestion and LC/MS analysis. The protein quantification is
carried out on the protein level. In addition, SILAC approaches are well suited for monitoring
changes in post-translational modifications. Examples for these applications include the
measurement of changes in protein phosphorylation and methylation.
Advantage of our SILAC technique:
Cutting-edge facilities & optimized protocols
Harmless in vivo labeling
Compare up to three samples at one time
High sensitivity
Suitable for study of protein-protein-interaction
Post-translational modification is detectable
Bioinformatic Analysis:
Functional annotation and enrichment analysis
Clustering analysis
Network analysis
Statistical analysis