This document outlines the requirements for testing seeds and grains including chick peas, lentils, and various types of gram and rice seeds in October 2018 at room temperature. The tests will utilize Coomassie Brilliant Blue G-250, methanol, phosphoric acid, Tris-HCl buffer, and double distilled water reagents as well as laboratory equipment like a single beam spectrometer, centrifuge, refrigerator, eppendorf tubes, cuvettes, beakers, pestle and mortar, wash bottle, micropipette, tips, reagent bottles, and Whatman filter paper.
This document outlines the requirements for testing seeds and grains including chick peas, lentils, and various types of gram and rice seeds in October 2018 at room temperature. The tests will utilize Coomassie Brilliant Blue G-250, methanol, phosphoric acid, Tris-HCl buffer, and double distilled water reagents as well as laboratory equipment like a single beam spectrometer, centrifuge, refrigerator, eppendorf tubes, cuvettes, beakers, pestle and mortar, wash bottle, micropipette, tips, reagent bottles, and Whatman filter paper.
This document outlines the requirements for testing seeds and grains including chick peas, lentils, and various types of gram and rice seeds in October 2018 at room temperature. The tests will utilize Coomassie Brilliant Blue G-250, methanol, phosphoric acid, Tris-HCl buffer, and double distilled water reagents as well as laboratory equipment like a single beam spectrometer, centrifuge, refrigerator, eppendorf tubes, cuvettes, beakers, pestle and mortar, wash bottle, micropipette, tips, reagent bottles, and Whatman filter paper.
Tester Materials: Seeds of chick peas, lentils, Bengal gram,
Green gram, Black gram, grains, wheat and rice Tester month and temperature: October,2018 and at room temperature Tester Reagents Coosmassie Brilliant Blue G-250 Methanol Phosphoric Acid (𝐻3 𝑃𝑂4 ) Tris_ HCl buffer Doubled distilled water Miscellaneous i. Single beam VIS Spectrometer ii. Cooling Centrifuge iii. Refrigerator iv. Eppendorf tube v. Cubette vi. 10 ml beaker vii. Pestie mortar viii. Wash bottle ix. Micropipette x. Tips of micropipette xi. Reagent bottle dark xii. Whatmann no. 1 filter papper