Data aktifitas Antioksidan

PERLAKUAN A.SAMPEL A.BLANKO

80 watt 1 menit 1:10 0.165 0.563
80 watt 1 menit 1:20 0.162 0.563
80 watt 1 menit 1:30 0.166 0.563
80 watt 5 menit 1:10 0.11 0.563
80 watt 5 menit 1:20 0.117 0.563
80 watt 5 menit 1:30 0.121 0.563
80 watt 10 menit 1:10 0.162 0.563
80 watt 10 menit 1:20 0.168 0.563
80 watt 10 menit 1:30 0.165 0.563

LAMA EKSTRAKSI (menit) RASIO (gram:ml) TOTAL FENOL (ml/mg

1 1:10 105.76
1:20 136.30
1:30 78.98
5 1:10 215.70
1:20 336.45
1:30 256.11
10 1:10 95.42
1:20 230.27
1:30 97.77

RASIO (gram:ml) LAMA EKSTRAKSI (menit) Total fenol (ml/mg)

1:10 1 105.76
5 215.70
10 95.42
1:20 1 136.30
5 336.45
10 230.27
1:30 1 78.98
5 256.11
10 97.77

Penentuan lama ekstraksi

No Lama Ekstraksi (menit) Total Fenol (mg/ml)
1 1 107.01
2 5 269.42
3 10 141.15

Variabel Bv Bn

Antioksidan 1 0.67
Fenol 0.5 0.33
Total 1.5 1

Penentuan rasio ekstraksi

No Rasio Ekstraksi Total Fenol (mg/ml)
 1 10 138.96
2 20 234.34
3 30 144.29

Variabel Bv Bn

Antioksidan 1 0.67
Fenol 0.5 0.33
Total 1.5 1
AKTIFITAS ANTIOKSIDAN (%)
70.69
71.45
70.52
79.22
79.88
78.51
70.85
70.22
70.69

RATA-RATA TOTAL FENOL (ml/mAKTIFITAS ANTIOKSIDAN (%) RATA-RATA AKTIFITAS ANTIOKSIDAN (%)
107.01 70.69 70.89
71.45
70.52
269.42 79.22 79.20
79.88
78.51
141.15 70.85 70.59
70.22
70.69

Rata-Rata Total Fenol (ml/mg) Aktifitas Antioksidan (%) Rata-Rata Aktifitas antioksidan (%)
138.96 70.69 73.59
79.22
70.85
234.34 71.45 73.85
79.88
70.22
144.29 70.52 73.24
78.51
70.69

aktifitas Aktioksidan (%) 1

70.89 0.30
79.20 8.62
70.59 0.035

1 5
Ne Nh Ne
0.03 0.02 1
0 0 1
0.02

aktifitas Aktioksidan (%) 10

 73.59 0.00
73.85 95.38
73.24 0

10 20
Ne Nh Ne
0.57 0.38 1.00
0 0 1
0.38
TAS ANTIOKSIDAN (%)

ntioksidan (%)

5 10 1 5 10
8.62 0.00 0.00 162.41 34.14
8.62 8.62 162.41 162.41 162.41
1 0 0 1 0.21

10
Nh Ne Nh
0.67 0.00 0
0.33 0.21 0.07
1.00 0.07

20 30 10 20 30
 95.38 5.32 0.35 0.61 0.00
95.38 95.38 0.61 0.61 0.61
1.00 0.06 0.57 1.00 0

30
Nh Ne Nh
0.67 0.00 0
0.33 0.06 0.02
1.00 0.02
Perlakuan A.Sampel A.Blanko Aktifitas Antioksidan (%)
2 menit 1:15 0.151 0.563 73.18
2 menit 1:25 0.148 0.563 73.71
8 menit 1:15 0.149 0.563 73.53
8 menit 1:25 0.142 0.563 74.78
5 menit 1:20 0.11 0.563 80.46
5 menit 1:20 0.114 0.563 79.75
5 menit 1:20 0.111 0.563 80.28
5 menit 1:20 0.117 0.563 79.22
5 menit 1:20 0.118 0.563 79.04

Perlakuan Total Fenol (ml/mg)

2 menit 1:15 136.46
2 menit 1:25 139.65
8 menit 1:15 139.40
8 menit 1:25 149.45
5 menit 1:20 330.81
5 menit 1:20 325.98
5 menit 1:20 329.93
5 menit 1:20 325.65
5 menit 1:20 325.17

Lama ekstraksi Rasio bahan dan pelarut (gram:ml Respon

aktifitas Antioksidan (%) Total Fenol (ml/mg)
2 1:15 73.18 136.46
2 1:25 73.71 139.65
8 1:15 73.53 139.40
8 1:25 74.78 149.45
5 1:20 80.46 330.81
5 1:20 79.75 325.98
5 1:20 80.28 329.93
5 1:20 79.22 325.65
5 1:20 79.04 325.17
ntioksidan (%)
Variabel-variabel kode serta Respon Hasil Percobaan untuk Total Fenol
X1 X1^2 X1Y X2 X2^2 X2Y Y
-1 1 -136.46 -1 1 -136.46 136.46
-1 1 -139.65 1 1 139.65 139.65
1 1 139.40 -1 1 -139.40 139.40
1 1 149.45 1 1 149.45 149.45
0 0 0.00 0 0 0.00 330.81
0 0 0.00 0 0 0.00 325.98
0 0 0.00 0 0 0.00 329.93
0 0 0.00 0 0 0.00 325.65
0 0 0.00 0 0 0.00 325.17
0 4 12.74 0 4 13.24 2202.5

b0 244.72
b1 3.19
b2 3.31

DB JK KT F hitung F tabel
0.05 0.01
R (b1, b2, b0) 2 84.40 42.20 0.00 5.14 10.92
Galat 6 4311930.94 718655.16
SDM 2 4311902.91 2155951.45 307613.00 6.94 18
GM 4 28.03 7.01
Total 8 4312015.34

Langkah-langkahX1 X2 Waktu (menit) Rasio (g/ml) total fenol (ml/mg)

Basis 0 0 5 0.06 ulangan 1 ulangan 2
Δ 1 1.04 2 2.08
Basis + Δ 1 1.04 7 21.74 288.06 292.76
Basis + 2Δ 2 2.08 9 23.48 296.51 299.80
Basis + 3Δ 3 2.61 11 25.22 96.36 95.42
Basis + 4Δ 4 3.48 13 26.96 100.12 102.00

0.65 379.47
296.51381319 299.802669 0.72 420.65
288.05675625 292.755121 0.67 393.00
0.66 385.35
Y^2
18621.33
19502.12
19432.36
22335.30
109435.26
106262.96
108855.45
106047.92
105735.53
4851017.26

JKR 84.4013

FK 539001.918 db reg 2

JKT 4312015.34 JKG 4311930.94

db tot 8
db galat 6

JKGM 536337.122 536309.1 db GM

28.0346

rata-rata
JKSDM 4311902.91
290.41
298.16 db SDM 5 0.22 125.35 100.12
95.89 0.21 120.65 96.36
101.06 0.62 360.65 288.06
0.64 371.24 296.51
303.09
335.98
313.90
307.79
4
 Variabel-variabel kode serta Respon Hasil Percobaan untuk Aktivitas Antioksidan
X1 X1^2 X1Y X2 X2^2 X2Y Y
-1 1 -73.18 -1 1 -73.18 73.18
-1 1 -73.71 1 1 73.71 73.71
1 1 73.53 -1 1 -73.53 73.53
1 1 74.78 1 1 74.78 74.78
0 0 0.00 0 0 0.00 80.46
0 0 0.00 0 0 0.00 79.75
0 0 0.00 0 0 0.00 80.28
0 0 0.00 0 0 0.00 79.22
0 0 0.00 0 0 0.00 79.04
0 4 1.42 0 4 1.78 693.96

b0 77.10677
b1 0.35524
b2 0.44405

Daftar Analisis Ragam

Sumber Keragama DB JK KT F hitung F tabel
0.05 0.01
R (b1, b2, b0) 2 1.29 0.65 0.05 5.140 10.920
Galat 6 80.38 13.40
SDM 2 78.81 39.40 99.92 6.940 18.000
GM 4 1.58 0.39
Total 8 81.68 0.00

Langkah-langkah X1 X2 Waktu (menit)Rasio (g/ml) Aktivitas antioksidan (%)

Basis 0 0 5 1:20 ulangan 1 ulangan 2
Δ 1 1.25 2 2.5 0 0
Basis + Δ 1 1.25 7 22.5 72.29 71.76
Basis + 2Δ 2 2.5 9 25 76.55 76.02
Basis + 3Δ 3 3.75 11 27.5 69.80 69.27
Basis + 4Δ 4 5 13 30 67.14 67.50
vitas Antioksidan
Y^2
5355.22
5433.50
5407.34
5591.75
6474.10
6360.27
6445.55
6275.57
6247.46
53590.76

FK 53509.08 JKR

JKT 81.68 DB REG

DB TOT 8 DB GALAT

JKGM 31802.95 31801.37

1.58

DBGM 4

JKSDM 78.81

DBSDM 2

ntioksidan (%) Perlakuan ulangan 1 ulangan2

rata-rata a.sampel a.blanko Aktifitas Antioksidan(%)a.sampel a.blanko
0 9 menit 0.132 0.563 76.55 0.135 0.563
72.02 7 menit 0.156 0.563 72.29 0.159 0.563
76.29 11 menit 0.17 0.563 69.80 0.173 0.563
69.54 13 menit 0.185 0.563 67.14 0.183 0.563
67.32
1.293502 JKG 80.3829

R2 0.015837

76.02
71.76
69.27
67.50
1 menit 2 menit 5 menit 7 menit 9 menit 11 menit 2 73.44583
73.1794 79.75 76.55 5 80.015
73.71226 80.28 76.02 22.5 7 76.285 298.16
25 9 72.02487 290.41
27.5 11 69.53819 95.89
30 13 69.53819 101.0618

R 78
R 78 e
e r 76
r 76 a
a t f(x) = − 1.13635568383659
t f(x) = − 0.909084547069274 x + 95.7100301953819 a 74 R² = 0.849858081573343
a 82 a 74 R² = 0.849858081573345

hubungan
k
t 80 lamaAekstraksi 72 dan aktifitas antioksidan A
k
72
k Column It 70
i t
70 i
Linear (Column I)
i
f 78 f
f
i 68
i f(x) = it− 0.682887476881947
68
x + 78.8237967808683 t
t 76 R² = 0.449995859275308
a 66
a
s
66
6 7 8 9 10 11 12 1
a 74 s 22 23 24 25 26 27 28 29 30 31
s A Waktu Ekstraksi (menit)
A Rasio Bahan : Pelarut (g:ml) n
72 n Column I t
a t i
i Column I o
n 70 o k
t k Linear (Column I) s
s i
i 68 i d
o d a
k 66 a
n
n

s 64 (
( %
i
d 0 2 4% ) 6 8 10 12 14 )

a waktu ekstraksi
n
T 350.00
T350.00 o
o t 300.00 f(x) = − 31.4320616425
t 300.00 a R² = 0.8043770117917
f(x) = − 39.2900770531855 x + 589.280680323247 l 250.00
a R² = 0.804377011791728
l 250.00
F 200.00
F 200.00 e
e Column J n 150.00
n 150.00 Linear (Column J) o
o l 100.00
l 100.00
( 50.00
( 50.00 p
p p 0.00
p m 22 23 24 25 26 27 2
0.00 )
m
6 7 8 9 10 11 12 13 14 Rasio Bahan : Pelarut (g
) Waktu ekstraksi (menit)
 f(x) = − 1.13635568383659 x + 83.2101176731794
R² = 0.849858081573343

Column I
Column I
Linear (Column I)

7 8 9 10 11 12 13 14
Waktu Ekstraksi (menit)

0.00

0.00 f(x) = − 31.4320616425484 x + 1021.47152790829

R² = 0.804377011791728
0.00

0.00

0.00 Column J
Linear (Column J)
0.00

0.00

0.00
22 23 24 25 26 27 28 29 30 31
Rasio Bahan : Pelarut (g:ml)