FEU CAVITE

MetroGate Silang Estates, Silang, Cavite Laboratory Experiment

SENIOR HIGH SCHOOL DEPARTMENT
S.Y. 2018-2019 2nd SEMESTER
No. 3

Name/s: Score:

Section: Group #:

Date Performed: Date Submitted: Teacher:

Paper Chromatography
Experiment Title

I. Objectives: (list down 3 objectives)

II. Materials: (with pictures)

III. Outlined Procedure: (concept map about the procedure, with pictures)

IV. Data and Results

SOLVENT USED: WATER
No. of components Distance migrated
Distance migrated Retardation
Color of Pen observed and their by each component,
by solvent, Ds (cm) factor, Rf
color Dc (cm)

BLACK

BLUE

GREEN

RED
SOLVENT USED: ACETONE
No. of components Distance migrated
Distance migrated Retardation
Color of Pen observed and their by each component,
by solvent, Ds (cm) factor, Rf
color Dc (cm)

BLACK

BLUE

GREEN

RED

SOLVENT USED: ALCOHOL

No. of components Distance migrated
Distance migrated Retardation
Color of Pen observed and their by each component,
by solvent, Ds (cm) factor, Rf
color Dc (cm)

BLACK

BLUE

GREEN

RED

Paste your dry chromatograms here.

WATER
 ACETONE

ALCOHOL

V. Guide Questions: (3-5 sentences only)

1. What are the general components of a chromatography?
2. What did you observe in the filter paper?
3. What observation would show that the ink is a mixture?
4. Differentiate your results based on the liquid used and color of pen used.
5. Which color travelled farthest with the mobile phase? What does this indicate about that
component’s attraction to the mobile phase? To the stationary phase?
6. What is the importance of solvents in a chromatography? Determine the polarity of the given
solvents in the experiment.
7. What is the importance of finding the retardation factor in chromatography?
8. How does paper chromatography be of use to consumers in testing food products?

VI. Conclusion/s: