ATP-Dependent Chromatin

Remodeling Complexes
ATP-DEPENDENT CHROMATIN REMODELING COMPLEXES

SWI/SNF Family

leus BAF Complex

Nuc
(SWI/SNF-A)
PBAF Complex
(SWI/SNF-B) Other SWI/SNF
Complexes:
BAF47
BAF53A BCL11A/B SS18 • Neural progenitor
SS18 BAF53A/B
BAF60A/B/C BAF
BRD9
ARID1A/B
β-actin
PBRM1 • Neuronal BAF
BAF57 ARID2
BAF45A/D BAF170
BAF47 • Embryonic stem
BAF57
BAF155 BAF170 cell BAF
BAF45A
BAF60A/B/C BCL7A/B/C BAF155
BRD7
BRG1 or β-actin
BRM BRG1

nucleosome

INO80 Family
Human INO80 Complex
CHD/mi-2 Family
NuRD Complex
ISWI Family INO80D
TFPT
NURF Complex INO80E
RBAP46 ARP4,5,8
HDAC1,2 MBD2,3 MCRS1 RUVBL1,2
RBAP48 RBAP48 INO80B UCHL5 INO80C
RBAP46 MTA1,2,3 p66α/β NFRKB YY1
BPTF
SNF2L CHD3,4 INO80
Me

CHRAC Complex ACF Complex Other ISWI Complexes: ATPase subunits are highlighted in red.
• NoRC Shared subunits are highlighted in orange.
Variable subunits are highlighted in blue.
ACF1 ACF1 • RSF
CHRAC17 CHRAC15 • WICH
SNF2H SNF2H

For full listing of antibodies, related kits, and reagents please visit

www.cellsignal.com/adcr
 ATP-Dependent Chromatin Remodeling Complexes
Visit www.cellsignal.com/adcr for full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY ANTIBODIES APPLICATIONS REACTIVITY

#6255 ACF1 Antibody W H, Mk #5681 IWS1 Antibody W, IP, IF-IC H, M, R
#12354 ARID1A/BAF250A (D2A8U) Rabbit mAb W, IHC-P H, M, R, Mk #5647 MTA1 (D40D1) XP® Rabbit mAb W, IHC-P H, M, R, Mk
#14820 ATRX (D1N2E) Rabbit mAb W, IP, ChIP H #5646 MTA1 (D17G10) Rabbit mAb W, IP H, M, R, Mk
#49360 Brg1 (D1Q7F) Rabbit mAb W, IP, ChIP H, M, R, Mk #12483 SMARCA1 (D4Q7V) Rabbit mAb W, IP H, Mk
#3508 Brg1 (A52) Antibody W, IF-IC H, M, Mk, (R) #9450 SMARCA1 Antibody W, IP H
#3514 Brg1 (P680) Antibody W H, M, R, Mk #12458 SMARCAD1 Antibody W H (B, Pg)
#11966 BRM (D9E8B) XP® Rabbit mAb W, IP, IF-IC, ChIP H, Mk, (Dg) #11956 SMARCC1/BAF155 (D7F8S) Rabbit mAb W, IP, ChIP H, M, R, Mk
#6889 BRM Antibody W H, Mk, (Dg) #12760 SMARCC2/BAF170 (D8O9V) Rabbit mAb W, IP, ChIP H, M, R, Mk, (Dg,
Pg, GP, Hr)
#4351 CHD1 (D8C2) Rabbit mAb W, IP, ChIP H, M, R, Mk, (B,
Dg, Pg) #8829 SMARCC2/BAF170 Antibody W H, M, R, Mk, (Hm, B,
Dg, Pg, GP, Hr)
#13460 CHD1L (E1I8C) Rabbit mAb W, IP H
#62265 SMARCD3/BAF60C (D6F1S) Rabbit mAb W, IP H, M, R, Mk
#4170 CHD2 Antibody W H, M, R, Mk
#38410 SNF2H (D4W6N) Rabbit mAb W H
#4241 CHD3 Antibody W, IP H, M, Mk
#8745 SNF5 (D9C2) Rabbit mAb W H, M, R, Mk, (Hm,
#11912 CHD4 (D8B12) Rabbit mAb W, IP H, M, R, Mk, (B, Pg,
C, X, B)
GP, Hr)
#21792 SS18 (D6I4Z) Rabbit mAb W IP ChIP H, M, R, Mk, (Dg, Hr)
#12011 CHD4 (D4B7) Rabbit mAb W, IF-IC, ChIP H, M, R, Mk, (Hm,
B, Pg, GP, Hr)
RELATED PRODUCTS
#4245 CHD4 Antibody W H, M, R (Mk , B, Hr) #8349 NuRD Complex Antibody Sampler Kit WB H, M, R
#44829 CHD5 (D2F9Q) Rabbit mAb WB, IP, IHC-P H #12854 SWI/SNF Complex Antibody Sampler Kit WB, IP H, M, R, Mk
#6505 CHD7 (D3F5) Rabbit mAb W, IP H, M #9003 SimpleChIP® Enzymatic Chromatin IP Kit ChIP
#11891 CHD8 (D3C1) Rabbit mAb W, IP H (Magnetic Beads)
#7656 CHD8 Antibody W H #9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit ChIP
(Magnetic Beads)
#7998 HELLS Antibody W H, M, Mk
#7017 6-Tube Magnetic Separation Rack IP, ChIP
#12463 HIRA (D6O8L) Rabbit mAb W, IP H, M, R, Mk
#13307 HIRA (D2A5E) Rabbit mAb W H, M, Mk

SMARCC1/BAF155 (D7F8S) Rabbit mAb #11956

Normal Rabbit IgG #2729
0.0035

Signal relative to input

0.003
0.0025
0.002
0.0015
0.001
0.0005
0
ESR1 pS2 α-Satelite

ARID1A/BAF250A (D2A8U) Rabbit mAb
#12354: Immunohistochemical analysis of
mouse colon using ARID1A/BAF250A (D2A8U)
Rabbit mAb.
BRM (D9E8B) XP® Rabbit mAb #11956: Brg1 (D1Q7F) Rabbit mAb #49360:
Confocal immunofluorescent analysis of HeLa Chromatin IPs were performed with cross-
(positive, left) and NCCIT (negative, right) linked chromatin from 4 x 106 MCF7 cells,
cells using BRM (D9E8B) XP® Rabbit mAb grown in phenol red-free medium and 5%
(green) and β-Actin (8H10D10) Mouse mAb charcoal-stripped FBS for 4 d followed by
#3700 (red). treatment with β-estradiol (10 nM, 45 min),
and either 10 μl of #49360 or 2 μl of #2729
using SimpleChIP® Enzymatic Chromatin IP
Kit (Magnetic Beads) #9003 and primers to
the designated loci.
SMARCC1/BAF155 (D7F8S) Rabbit mAb
#11956: Chromatin IPs were performed with
cross-linked chromatin from 4 x 106 MCF7
cells, grown in phenol red-free medium and
5% charcoal-stripped FBS for 4 d followed by
treatment with β-estradiol (10 nM, 45 min),
and either 5 μl of #11956 or 2 μl of #2729
using SimpleChIP® Enzymatic Chromatin IP
Kit (Magnetic Beads) #9003 and primers to
the designated loci.

CHD5 (D2F9Q) Rabbit mAb #44829:

SS18 (D6I4Z) Rabbit mAb #21792: Immunohistochemical analysis of paraffin-
Western blot analysis of extracts from various embedded normal human testis using CHD5
cell lines using SS18 (D6I4Z) Rabbit mAb. (D2F9Q) Rabbit mAb.
16EPIGADCR126ENG

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry
/ -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all
species expected / ( ) 100% sequence homology

© 2016 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, PathScan, SimpleChIP, and XP are trademarks of Cell Signaling Technology, Inc. Patent No. 5,675,063. All other trademarks are the property of their respective owners.

For Research Use Only. Not For Use In Diagnostic Procedures.

www.cellsignal.com/adcr