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Hormone and Seed Germination
Hormone and Seed Germination
The experiment will be undertaken to: 1. Prepare 6 petri plates with blotting papers. Place 3
seeds of mustard in each petri plate. This is good
1. determine the effects auxin and gibberellin in the for 3 treatments with one replicate each.
seed, root and shoot development of mustard plant
2. explain how the action of each hormone affect each 2. Label the petri plates with A 1, A2 (replicate), B1,
other based on appearance and development of plant and B2 (replicate) and C1and C2 (replicate).
3. explain how different concentrations of IAA and 3. Add 1 little drop of 0.1 mg/L GA to each seed of
GA affect the growth of the root and shoot growth
both A plates.
4. Add 1 a drop of 0.1 mg/L IAA to each seed of
both B plates.
5. Then, Add a drop of 0.1 mg/L IAA and 0.1 mg/L
GA to each seed of both C plates.
6. Observe the germination of seeds after 3 days.
Count the seeds that complete germinated after 3
days. Check the appearance of each seed and
compare it to each other. Regularly wet the seeds
References:
with one drop of the designated hormone in each
day. 1. Eiichi Tanimoto (2005): Regulation of Root
Growth by Plant Hormones—Roles for Auxin
and Gibberellin, Critical Reviews in Plant
Sciences, 24:4, 249-265
B. The Role of IAA and GA on root formation
and shoot formation 2. Previous experiment – Hormonal Regulation
in Plants
1. Remove the grown plantlets from the soil. Take a
photo of the appearance of the plantlet stem,
leaves and roots before adding the treatments.
Measure the initial height of the plant from the
roots to the seed.
2. Cut the roots from the base of the hypocotyl.
Immersed the 3 plantlets in each plastic cup. For
each plastic cup there are different concentration
of hormones.
A- With 0.1 mg/l GA and IAA
B- With 1.0 mg/L GA and IAA
C- With 10.0 mg/LGA and IAA
0.1 mg/L GA
Expected results:
Greater percentage of seed germination will occur in both IAA and GA treatment. Combined function of
both hormones promotes growth and development of each tissue of the plant. GA may have affected
the germination and consumption of food storage in seed and elongation of its stem, while the IAA the
application of exogenous level promotes the growth and absorbing capacity of the developing roots.
Expected results:
Both hormones affect each other the treatment C will yield the greatest no. of roots and taller shoots than
previous concentrations because the high level of gibberellic acid could increase the IAA function by increasing
in cell wall plasticity and extensibility to allow cellular growth and development.
Normal condition
Heated at 70 o C
Expected Results:
Germination will be obviously seen in heated seeds. Heat will stimulate mechanical damage to the seed coat and
induce germination when treated with suitable watering.
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