3/4/2019 Sperm test ( semen analysis ) report - a patient's guie

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Sperm test ( semen analysis ) report - a patient's guie

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Dr Aniruddha Malpani, Angel Investor. Malpani Ventures. Funding frugal innovation in India.
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Published on May 7, 2015

A patient's guide to making sense of the sperm test ( semen analysis ) report.

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Published in: Health & Medicine

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Sperm test ( semen analysis ) report - a patient's guie

1. 1. SEMEN TEST CHEMICAL EXAMINATION MICROSCOPIC EXAMINATION Motility of spermatozoa

: (at 37 C) Method of collection: Manipulation Spillage : Nil Continence : 4 days Appearance : Milky white Self
Liquification : Occured in 20 minutes Volume : 2 ml Fructose: Positive Rapid Linear Progressive Sluggish Linear
Progressive Total Forward Progressive Non Progressive Non Motile Nature of Motility (W.H.O) MacLeod
Grade After 1/2 hours After 2 hours pH: 7.5 Transparency: Translucent Viscosity : Normal Specimen produced at
: 12.55 p.m Specimen examined at : 1.25 p.m Sperm Count: The number of spermatozoa per ml: 5 Millions. Total
number of sperms per ejaculate : 10 Millions. Viability: 10% of spermatozoa alove at the end of 1/2 hours A IV &
III II I O A+B C D B Active sperms in the ejaculate ( at the end of 1/2 hours) : 0.5 millions Dr. Aniruddha
Malpani Dr. Anjali Malpani Malpani Infertility Clinic. Jamuna Sagar, Shahid Bhagat Singh Road, Colaba, Bombay
400 005. Tel: 91-22-22151065, 91-22-22151066 www.drmalpani.com Email: info@drmalpani.com -% -% 5%
12% 5% 5% -% 2% 2% 2%
2. 2. SPERMIOGRAM ( Sperm morphology or sperm shape and form) CELLULAR ELEMENTS
MISCELLANEOUS CHARACTERS & BACTERIOLOGICAL EXAMINATION Abnormalities of Head Ab.
of Neck Ab. ofTail Giant heads : 6 % Bent neck : 4 % Short tail: 6 % Microsperms : 2 % Thick neck : - %
Double tail: 2 % Pin heads : 6 % Cytoplasmic Absent tail: 4 % Round heads : 4 % Appendages : 4 % Curled tail:
- % Tapering : 6 % Double head : 4 % Miscellaneous Ragged edges : - % Immature : - % Deformed : 4 %
Duplicate : - % Multinucleated : - % Terratoid : - % Acrosome Absent : - % Nucleus Absent : - % Total number
of abnormal spermatozoa : 52% Epithelial Cells: Squamous type Macrophage Cells: Occassional Pus cells:
Candida : Nil Red Blood Cells: Trichomonads : Nil Precursors : Approximate number per ml Granular debris:
Present + Crystal : ------ Agglutination of sperms: - Grams stain : No pathogen seen Dr. Aniruddha Malpani Dr.
Anjali Malpani Malpani Infertility Clinic. Jamuna Sagar, Shahid Bhagat Singh Road, Colaba, Bombay 400 005.
Tel: 91-22-22151065, 91-22-22151066 www.drmalpani.com Email: info@drmalpani.com
3. 3. Dr. Aniruddha Malpani Dr. Anjali Malpani Malpani Infertility Clinic. Jamuna Sagar, Shahid Bhagat Singh Road,
Colaba, Bombay 400 005. Tel: 91-22-22151065, 91-22-22151066 www.drmalpani.com Email:
info@drmalpani.com NORMAL SEMEN PARAMETERS * CORELATION OF SPERM MOTILITY AS
PER W.H.O CRITERIA & MCLEOD GRADES NORMAL SEMEN PARAMETERS : W.H.O MANUAL
1999 ( FOURTH EDITION) Volume: 2 ml or more pH : 7.2 - 8.0 Viability : 75% or more Sperm concentration :
20 millions per ml or more Total sperm count : 40 millions per ejaculate or more Morphology : 30% or more with
normal morphology Motility : 25% or more with Rapid Linear Progressive activity (A) OR 50% or more with
forwards progressive motility (A+B) - Within 1 hour of collection (A) Rapid Linear Progressive Motility includes
Macleod’s Grades IV & III Grade IV : Excellent Swift Progressive Motility Grade III : Moderate Progressive
Motility (B) Sluggish Linear Progressive Motility corresponds Macleod’s Grades II Grade II : Struggling
progressive Motility (C) Non Progressive Motility corresponds Macleod’s Grades I Grade I : Poor Sluggish Non
Progressive Motility
4. 4. Dr. Aniruddha Malpani Dr. Anjali Malpani Malpani Infertility Clinic. Jamuna Sagar, Shahid Bhagat Singh Road,
Colaba, Bombay 400 005. Tel: 91-22-22151065, 91-22-22151066 www.drmalpani.com Email:
info@drmalpani.com Putting it all together, one looks for the total number of "good" sperm in the sample - the
product of the total count, the progressively motile sperm and the normally shaped sperm. This gives the

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progressively motile normal sperm count which is a crude index of the fertility potential of the sperm. Thus, for
example, if a man has a total count of 40 million sperm per ml; of which 40% are progressively motile, and 60%
are normally shaped; then his progressively motile normal sperm count is : 40 X 0.40 X 0.60 = 9.6 million sperm
per ml. If the volume of the ejaculate is 3 ml, then the total motile sperm count in the entire sample is 9.6 X 3 =
28.8 million sperm.This particular report is very abnormal. The count is only 5 million per ml and the motility is
only 5%. This infertile man will need ICSI if he wants to have a baby. A normal sperm report is reassuring, and
usually does not need to be repeated. If the semen analysis is normal, most doctors will not even need to examine
the man, since this is then superfluous. However, remember that just because the sperm count and motility are in
the normal range, this does not necessarily mean that the man is "fertile". Even if the sperm display normal motility,
this does not always mean that they are capable of "working" and fertilising the egg. The only foolproof way of
proving whether the sperm work is by doing IVF (in vitro fertilisation ) ! Poor sperm tests can result from
incorrect semen collection technique, if the sample is not collected properly, or if the container is dirty too long a
time delay between providing the sample and its testing in the laboratory too short an interval since the previous
ejaculation recent systemic illness in the last 3 months (even a flu or a fever can temporarily depress sperm counts)
If the sperm test is abnormal, this will need to be repeated 3-4 times over a period of 3-6 months to confirm
whether the abnormality is persistent or not . Don't jump to a conclusion based on just one report - remember that
sperm counts do tend to vary on their own ! It takes six weeks for the testes to produce new sperm - which is
why you need to wait before repeating the test. It also makes sense to repeat it from another laboratory to ensure
that the report is valid. If you do have a low sperm count, you can read more about this at
http://issuu.com/malpani/docs/how_to_have_a_baby_chp_7 There are a number of additional sperm functions
tests also available. These include: Antisperm Antibodies Test, Semen Culture Test, Postcoital Test (PCT), Bovine
Cervical Mucus Test, Sperm Viability or Sperm Survival Test; Sperm DNA fragmentation tests; Sperm Chromatin
Structure Assay Test; Sperm FISH tests. Many of these are expensive tests, which provide information of very
little clinical use. While they maybe of use in the research setting, they really do not answer the question the
infertile man is asking – are my sperm capable of getting my wife pregnant ? This is why we do not ask our
patients to do any of these useless tests. NEED HELP WITH INTERPRETING YOUR OWN REPORT ? Dr
Malpani will be happy to help . Please send me your medical details by filling in the form at
www.drmalpani.com/malpaniform.htm ! Taking treatment at a world-class clinic will maximize your chances of
success and give you peace of mind you did your best ! Conclusion

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