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STANDAR POLIFENOL (ASAM FERULAT)

KONSENTRASI ABSORBANSI
U1 U2 U3 MEAN Konsentrasi absorbansi
0 PPM 0.014 0.013 0.011 0.01267 0 0 0 0
20 PPM 0.11 0.109 0.104 0.10767 0.095 20 0.095 1
40 PPM 0.181 0.178 0.183 0.18067 0.168 40 0.168 2
60 PPM 0.252 0.265 0.272 0.263 0.25033 60 0.250333333 3
80 PPM 0.336 0.324 0.341 0.33367 0.321 80 0.321 4
100 PPM 0.413 0.417 0.419 0.41633 0.40367 100 0.403666667 5
125 PPM 0.517 0.505 0.519 0.51367 0.501 125 0.501 6.25
150 PPM 0.602 0.594 0.622 0.606 0.59333 150 0.593333333 7.5

0.7

0.6
f(x) = 0.0783909116x + 0.0098243281
0.5 R² = 0.9992964291

0.4

0.3

0.2

0.1

0
0 1 2 3 4 5 6 7 8
Kurva standar asam ferulat
0.7
0

Absorbansi
0.095 0.6 0
f(x) = 0.0039195456x + 0.0098243281
0.168 R² = 0.9992964291
0.5 0.501
0.250333
0.321 0.4 0.4036666667
0.403667
0.501 0.321
0.3
0.593333 0.2503333333
0.2
0.168
0.1 0.095

281 0 0
0 20 40 60 80 100 120 140
konsentrasi asam ferulat

Column K
Linear
(Column K)

6 7 8
am ferulat

0.5933333333

0.501

0.4036666667

0.321
3333

0 100 120 140 160


am ferulat
y = 0.003x + 0.009 y = 0.078x + 0.009

sampel Abs Abs rerata Sampel Parameter Abs. C1 (ppm) V1 (mL)

H2O2 1 0.372 0.371 H1U1 0.371 4.6410 10


0.368 H1U2 0.310 3.8590 10
0.373
H2O2 2 0.31 0.310
0.318 H1U1 0.371 120.6667 1
0.302 H1U2 0.310 100.3333 1
C
C polifenol
C stok polifenol (gram per
C polifenol (ppm per (gram
V2 (mL) C2 (ppm) ekstrak per gram mg/g GAE Rata2
1 ppm ekstrak)
(ppm) 1 gram 100 atau %
ekstrak) b/b)

0.5 92.8205 1000 0.0928 0.0928 9.282 92.821


0.5 77.1795 1000 0.0772 0.0772 7.718 77.179

1 120.6667 1000 0.1207 0.1207 12.067 120.667


1 100.3333 1000 0.1003 0.1003 10.033 100.333

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