HumaLyzer 3500

| Service Manual

|
Cat.No. 16801/2
 Revision List of the Manual
No. Rev. / Date REVISION DESCRIPTION
1 01/2006-12 First edititon

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NOTICE
Analytical instruments for in vitro diagnostic application involve the handling of human samples and controls
which should be considered at least potentially infectious. Therefore every part and accessory of the respective
instrument which may have come into contact with such samples must equally be considered as potentially
infectious.

BIOHAZARD

The „BIOHAZARD“ warning label must be affixed to instrument prior to first use with biological material !

Servicing Note:
Before doing any servicing on the instrument it is very important to thoroughly disinfect all possibly contaminated
parts. Before the instrument is removed from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination should be performed by authorised well-trained personnel only, observing all necessary safety
precautions. Instruments to be returned have to be accompanied by a decontamination certificate completed by
the responsible laboratory manager. If a decontamination certificate is not supplied, the returning laboratory will
be responsible for charges resulting from non-acceptance of the instrument by the servicing centre, or from
authority’s interventions.

HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
| Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
| Tel.: +49 61 22/99 88-0 · Fax: +49 61 22/99 88-100
| e-Mail: human@human.de · www.human.de

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 Contents

1 Introduction 3
2 Description of the Analyzer 3
2.1 Technical specifications 4
2.2 Rear panel setting 4
2.3 Location of the Instrument 5
2.4 Power supply connection 5
2.5 Caution 5
2.6 Measurement block 5
2.7 Tube setting 6
2.8 Incubator 7
2.9 Printer Settings 7
3 INSTRUMENT CALIBRATION 8
3.1 Display Contrast Regulation 8
3.2 Analogic Photometer Calibration (Set voltmeter range to milliVolt) 8
3.3 Tension regulation 8
4 SERVICE MENU: 9
4.1 FILTERS SEL. 10
4.1.1 Check rotation functionality of filter wheel 10
4.2 T. CUVETTE 11
4.2.1 Cuvette/Incubator temperature calibration 11
4.3 T.INCUBATOR 11
4.3.1 Cuvette/Incubator temperature calibration 11
4.4 READING ABS 12
4.5 Lamp Targeting 13
4.5.1 ABS Mode Measurement 13
4.5.2 Tension Mode Measurement 13
4.5.3 In case that it is not possible to obtain such tension values: 14
4.6 WASHING 14
4.7 DOWN LOAD 15
4.8 UPLOAD 15
4.9 CALIBRATION PUMP 16
4.9.1 Humalyzer Hydraulic Layout: 17
4.9.2 CALIBRATION TS (TOUCH SCREEN) 18
4.9.3 Recalibrate Touch Screen: 18
4.10 HOST 19
5 TESTING HumaLyzer 20
5.1 Check voltage test point (Voltmeter 20 Vcc) 20
5.1.1 Check continuos Voltage (TP2 is ground). 20
5.2 Check linearity 20
6 OPTICAL GROUP 20
 6.1 Incubator 21
6.2 Printer 21
7 CONNECTONS CABLES WITH THE BOARD 21
7.1 Appendix 1: Changing lamp 21
7.2 Appendix 3: Red tube changing 22
7.3 Touchscreen Board: 22
8 MAIN BOARD 23
8.1 Software and Firmware Upgrade 24
8.1.1 Managing Firmware upgrade for HumaLyzer 24
8.1.2 Diagram wiring for re-programming cable: 29
8.1.3 Peripherals Firmware upgrade for HumaLyzer 30
8.1.4 Program microprocessor (for XP) 30
8.1.5 Automatic Software Loading 30
8.1.6 Scheme Serial PC 31
8.1.7 Special Adapter to J28 31
8.2 Methods Parameter Download and Upload 32
8.2.1 Download Procedure 33
8.2.2 Upload Procedure 33
8.3 Procedure for Touch Screen Replacement 33
8.3.1 Check Calibration 35

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1 Introduction

The HumaLyzer 3500 is a semi-automated, programmable photometer intended for In Vitro Diagnostic (IVD) use by
qualified laboratory staff only.
For best results become familiar with the instrument and its capabilities before attempting any clinical diagnostic
tests. Refer any questions to your HUMAN distributor.

2 Description of the Analyzer

Technical features:

Touch screen with virtual alphanumeric keyboard.

Multilingual capability, up to 6 different languages (Including Chinese characters).
200 programmable tests.
1000 results storing capability categorised by date.
Intelligent patient assay management
Each test handles 3 different QC (quality control)
Built-in eight position incubation block.
Integrated pump and flow cell.
Programmable Aspiration features (With Re-run Capability)
Internal pre-adjusted Peltier element at 25°C, 30°C and 37°C.
16 different QC values storage capability.
25 QC archives with 100 result capability.

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2.1 Technical specifications

Graphic Display 320x240 (1/4 of VGA)

Patient name length 16 characters
Max test storage 1000 test
External interface PS2 external keyboard (connection on rear side of instrument)
RS232
Light source Halogen lamp 12V, 20W
Photo detector Silicon based (range 300-1000nm)
Wavelength 340nm-700nm.
Wavelength selection Automatic via 8-position filter wheel;
6 standard interference filters: 340nm, 405nm, 505nm, 546nm, 578nm, 630nm.
Two free positions for optional filters.
Photometric Range: 0-2.5 O.D.
Flow cell system 32L flow cell with 10mm light path, interchangeable with disposable macro, semi-
micro, or special optical glass cuvettes.
Reading time 1-999 seconds
Incubation time 5-999 seconds.
Temperature control Peltier elements, 25°C, 30°C and 37°C.
Reaction volume 500 L per test.
Printer Graphic, 29 characters per line.
Printing sort Batch, profile, reaction dynamics, QC print, Levey Jennings graph curves
Dimensions 30cm x 33cm x 37cm
Weight 8.4 kg
Power supply 110-230 AC 50-60 Hz

2.2 Rear panel setting

Located in the rear panel (from left to right as shown below) you will find:
1 Fan for internal cooling (a duplicate is located under the instrument).
2 The power supply switch.
3 The waste outlet.
4 RS232 connection
5 PS2 External standard keyboard connection

5) PS2 1) FAN 2) POLYSNAP and POWER SWITCH

3) OUTLET
4) RS232, IBM printer kompatibel

WASTE OUTLET: Before switching on the instrument, remember to connect the plastic outlet connector (red) to a
waste tank by means of a silicone tube.

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2.3 Location of the Instrument
The instrument should be located in a clean environment, placed on a stable surface, and away from direct
sunlight, which could affect the operating temperature and the quantity of light measured by the instrument.

The following points should be taken into consideration.

- Ensure that it is on a level surface.

- Avoid positions subject to jerks or vibrations.
- Make sure that the instrument is not placed close to air conditioning or heat sources.
- For the long life of the instrument these temperature conditions should be followed:
5°C - 50°C for instrument storage.
15°C - 30°C for instrument use.

2.4 Power supply connection

- Please check the setting of the power supply switch according to your country’s electrical network.
- Connect the power plug to a good grounded AC wall outlet, preferably one that is not shared with other
electric appliances and with low fluctuation of line voltage compared to the standard voltage specified
(10-15%).
- Keep the instrument away from other appliances that generate high frequency electrical noises (e.g.
radiological instruments).
- Before connecting the power cord, check that the AC power supply corresponds to the value that is
stated on the instrument’s label.

2.5 Caution
Do not connect the instrument to a power supply different from the value indicated on the label.

- Before connecting the power and finishing the installation section, make sure that the instrument is turned
off (check the polysnap located on the rear part of the instrument).
- Make sure that your AC main line has an efficient ground line. A bad ground line connection may
compromise analysis results and damage the instrument.
- After turning on the instrument, pay attention not to spill liquids or micro solid substances on the surface
around the instrument.
- Keep the instrument away from young children.

If the above procedures are carefully followed, it will be possible to TURN ON the instrument by using the switch
located on the polysnap block.

2.6 Measurement block

Open the measurement block cover lid, positioned on the top left part of the instrument. Inside you will see:

- Peristaltic pump that allows the solution to enter the instrument for measurement.

- Incubator well which keeps the flow-cell or manual cuvette at the temperature of approximately 37° C.

- Inlet tube.

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2.7 Tube setting

Make sure that the transparent tube (the one that connects the flow-cell with the internal hydraulic circuit) is not
twisted or squashed.

Place the red tube around the peristaltic pump and make sure that the white plastic connections are set as shown
in the figure below:

PUMP
INLET TUBE

RED TUBE

CUVETTE POSITION

Under the measurement block there is a lever (the PUSH button). Press the lever for no more than half a second to
pump samples into the flow cell.

MEASUREMENT

INLET PIPE
PUSH button

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2.8 Incubator
The incubator temperature is brought up to 37°C by the software and it will remain constant until the instrument
is turned off.

NOTE: It is very important to heat the macro cuvettes to the proper temperature for obtaining the most accurate
analysis results.

Built-in incubation block.

2.9 Printer Settings

To insert the paper roll, proceed as follows:

- Pull the lever to open the cover as shown in step 1

- Take a new paper roll, insert it as shown in step 2
- Pull the edge of the paper out of the printer as shown in step 3
- Close the cover as shown in step 4

Step 1 Step 2

Step 3 Step 4

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3 INSTRUMENT CALIBRATION

3.1 Display Contrast Regulation

- Switch on the instrument

- Regulate RV1 on the display interface board (under the TS display) to get a good contrast

3.2 Analogic Photometer Calibration (Set voltmeter range to milliVolt)

Switch on the instrument (for diagram refer to pag.26)

First Stage

- Shortcircuit JP1
- Connect the voltmeter between TP10 and TP2
- Regulate RV2 to read 0 volts
- Open JP1

Second Stage

- Shortcircuit JP2
- Connect the voltmeter between TP9 and TP2
- Regulate RV1 to read 0 volts
- Open JP2

3.3 Tension regulation

- Switch on the instrument

- Regulate the trimmer on the right side of the power supply so that between the yellow wire and the black
wire of J26 you measure about 13.5 Volt

For the Lamp targeting procedure please follow instructions on page 12

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4 SERVICE MENU:

In order to enter in service menu:

1) Switch on the instrument

2) From main menu, press in quick sequence the box number 1, 2 and 3 as shown in below figure:

1 2

The service menu will appear:

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This service menu will display different menus as follow:

1. FILTER SEL.
2. FILTER MOT.*
3. PUMP*
4. PUMP MOT.*
5. READING AD*
6. T. CUVETTE
7. T.INCUBATOR
8. WAIT PUSH*
9. READING ABS
10. WASHING
11. DOWN LOAD
12. UPLOAD
13. CALIBRATION PUMP
14. CALIBRATION TS (TOUCH SCREEN)
15. HOST

(*): This menus are dedicated for internal company testing procedure. No intended use for service.

4.1 FILTERS SEL.

This menu will allow:

4.1.1 Check rotation functionality of filter wheel

In order to rotate the filter wheel and set the selected filter wavelength under the light path, proceed as follow:

1. Press the “SEL. FILTER” message and the sub-menu will appear.
2. Use the right and left arrows to select a specific filter wavelength, and then press Exec.
3. The filter wheel will rotate in order to set the selected filter under the light path.
4. 340nm> no colour
5. 405> Violet
6. 505> Dark Green
7. 546> light Green
8. 578> Yellow
9. 630> Red

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4.2 T. CUVETTE

Press the “T. CUVETTE” message and the sub-menu will appear.
The 1° row stated as: “Temp. Cuvet” will show the flowcell current temperature.
In case the temperature need adjustment, proceed as follow:

4.2.1 Cuvette/Incubator temperature calibration

This screen allows you monitoring temperature gradients of incubator and cuvette according to temperature
displayed in user program and digital level acquired by microcontroller (both current and target).

- Dismantle screws of upper external case.

- Position thermometer’s probes into disposable macrocuvette filled with 0.5ml of water
- Switch on the instrument wait at least 20 minutes.
- Regulate RV4 incubator temperature level to obtain 38,5°C on the external thermometer.
- Regulate RV3 cuvette temperature level to obtain 37,5°C on the external thermometer.

The message: ”Temperature” will show the target temperature to be reached.

4.3 T.INCUBATOR
Press the “T.INCUBATOR” message and the sub-menu will appear.
The 1° row stated as: “Temp. Incub.” will show the Incubator block current temperature.
In case the temperature need adjustment proceed as follow:

4.3.1 Cuvette/Incubator temperature calibration

This screen allows you monitoring temperature gradients of incubator and cuvette according to temperature
displayed in user program and digital level acquired by microcontroller (both current and target).

- Dismantle screws of upper external case.

- Position thermometer’s probes into disposable macrocuvette filled with 0.5ml of water
- Switch on the instrument wait at least 20 minutes.
- Regulate RV4 incubator temperature level to obtain 38,5°C on the external thermometer.
- Regulate RV3 cuvette temperature level to obtain 37,5°C on the external thermometer.

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The message: ”Temperature” will show the target temperature to be reached into incubator block.

4.4 READING ABS

Press the “READING ABS” message and the sub-menu will appear.
In this menu it is possible to read the Photocell amplifier energy, to perform an Absorbance reading and to target
the lamp.

The sub-menu will show:

- Select Filter: The filter that operator want to set, use the right and left arrows to select: (340nm, 405nm,
505nm, 546nm, 578nm and 630nm)
- Actual Filter: The filter currently placed under the light beam
- Absorbance: The Abs value of the substance present in the flow cell.
- Tension: The mVolt value present on the Photodiode preamp.

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4.5 Lamp Targeting
DO NOT TOUCH THE LAMP DIRECTLY WITH THE HANDS!

1. Select filter 340, then press the wheel button and make aspiration with distilled water
2. Set the test tube of distilled water in the inlet pipe and press the PUSH button.
3. After a few seconds, continuous evolution of the tension value will appear on the display.
4. Loosen the screws of the lamp stirrup
5. Target the light bundle trough the reading hole to obtain the max tension on the display
6. Screw the screws

4.5.1 ABS Mode Measurement

1. Select filter with arrows then press wheel icon, the desired filter will be placed under the light beam
2. In order to obtain the Abs value make and aspiration with water and wait for measurement.
3. Set the test tube of water (or other substances) in the inlet pipe and press the PUSH button.
4. After a few seconds, continuous evolution of the ABS value will appear on the display.
5. Press 0 key to have zero on the current ABS value (water or other substances). You will choose this
option when you will set to zero the instrument.
6. Prepare a new sample (reagents, potassium-dichromate or others) and press the PUSH button to start a
new ABS measurement session.
7. At the screen exit, the instrument expels the volume from flow cell via the outlet waste.

4.5.2 Tension Mode Measurement

In “READING ABS” screen you can monitor (after having filled water) tension coming from optical group (OPT TENS)
and tension coming in A/D converter (A/D TENS) , using a particular filter (FILTER)

Tension Mode Measurement is very useful to verify lamp calibration, filter / lamp / lens / photodiode / preamp
board quality, position sensor working.
Moreover in this screen you can test correct pump motion.

- Press PUSH Button to aspirate distilled water

- To select one filter use the right and left arrows: (340nm 405nm, 505nm, 546nm, 578nm and 630nm)
- Press wheel icon, the desired filter will be placed under the light beam
- Look at all filters TENSION values.
- To change filter, press PUSH BUTTON if you want to fill again water.

If you have a correct calibrated optical group, when you use the flow cell, the TENSION values are between 250
mV (for 340 nm) and about 3.5 V (for 630 nm) for all filters.

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Regulate trimmer R1 to obtain 3,5 V on the Tension menu (see picture bellow):

Rear view of the preamp: R1

Trimmer to regulate tension

4.5.3 In case that it is not possible to obtain such tension values:

- Unscrew, to unblock the lamp

- Find the maximum voltage moving slightly in several directions.
- Then fix the lamp bloc with the screws

4.6 WASHING
When pressing this message the peristaltic pump will be activated for around 10 seconds.

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4.7 DOWN LOAD
Refer to the Methods Parameter Download and Upload paragraph.

4.8 UPLOAD
Refer to the Methods Parameter Download and Upload paragraph.

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4.9 CALIBRATION PUMP
Now the pump work following a determinate step numbers 4790 is the default pump step number for the
peristaltic pump, the instrument should aspirate correctly with this number.
In case the aspiration needs to be calibrated, operate as follow:
- Search with down scroll arrow the “Pump calibration” when highlighted press it the following screen
will appear:

The following values will be displayed:

Pump Calibration

Steps for 10 ml 4795

Steps for a.gap 50
Empty 1500

- Steps for 10 ml: Aspiration pump steps for 0.5ml

- Steps for a.gap: air gap pump steps
- Empty: Waste pump steps

In case check aspiration as follow:

- Exit from service and from main menu enter in setup menu
- Look for “Automatic empty” menu and insert 5000 value (air gap delay time, equal to 5 seconds), then
save. See also user manual.
- Enter in Run method and run glucose.
- Prepare 500 l of water in a cuvette set the aspiration tip in a bottom angle of manual cuvette and press
aspiration button.
- If the pump works correctly, the water should be aspired completely from the manual cuvette, and
water should stop at the top of the aspiration tip.
- After 5 seconds the air gap will be aspired and liquid front will rise up.
- Repeat operation several times

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4.9.1 Humalyzer Hydraulic Layout:

The aspiration tip should

Water stops here 10 cm
be empty from water after
from cuvette hose after
aspiring air gap.

Cuvette hose

Top of aspiration tip Peristaltic pump and Red Tube

Manual curvette Flow cell

After the complete aspiration (sample aspiration + air gap), if water stops after at beginning of aspiration tip or
stops to near the cuvette the red tube has a problem and you have to adjust aspiration by changing the aspiration
pump steps.

Aspiration regulation, proceed as follow:

1. Enter in service menu and open Pump calibration menu.

2. Increase “steps for 10ml” number to increase aspiration pump steps (the liquid will stop nearer to
cuvette). Use difference of +50 steps each try, then save.
3. Decrease “steps for 10ml” number to decrease aspiration pump steps (the liquid will stop further to
cuvette). Use difference of -50 steps each try, then save.
4. Exit from service.
5. Enter in Run method and run glucose
6. Prepare 500 l of water in a cuvette set the aspiration tip in a bottom angle of manual cuvette and press
aspiration button.
7. If the pump works correctly, the water should be aspired completely from the manual cuvette, and
water should stop at the top of the aspiration tip.
8. After 5 seconds the air gap will be aspired and liquid front will rise up.
9. Repeat operation several times
10. If the pump works correctly, the water should be aspired completely from the manual cuvette, and also
the aspiration tip should be empty from water:
11. Keep adjusting until the aspiration is correct from point 1

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4.9.2 CALIBRATION TS (TOUCH SCREEN)

Scroll down to the Touch screen Calibration menu and select it:

Inside menu Touch with a pencil and an indicator will appear in correspondence of the pencil tip.
The tip of the pencil must be in the same position of the indicator, try different position of touch, especially the one
where you report the problem on keyboard.

NOTE: DO NOT PRESS TEST BUTTON FOR ANY REASON!

In case the tip and indicator are too far, you need to recalibrate touch screen.
Press Door to exit

4.9.3 Recalibrate Touch Screen:

1. Press CALIB button

2. with the tip of pencil touch the middle of the flashing indicator until it disappear
3. Repeat this operation for all the indicator that will appear in sequence (16)
4. Exit and the tip of the pencil must be in the same position of the indicator, try different position of
touch, especially the one where you report the problem on keyboard.
5. If touch screen is now calibrated press SAVE button
6. If still touch screen is still needing adjustment repeat operation from point 1

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4.10 HOST

A Host (program manage Running under Windows XP and supplied separately) to operate the Humalyzer from an
external PC.

In the Host program it is located all the features of the HumaLyzer software, plus an extended database and a large
choice of printer will able to be used with Humalyzer.

In order to connect the Humalyzer to a Host Computer via RS232 proceed as follow:

1. Load setup of Host program in the external PC (RS232 required)

2. Connect RS232 to Humalyzer Enter in Service of Humalyzer and select the option Host
3. Run the Host program in the external PC
4. From this moment all commands of HumaLyzer will be delegated to Host PC

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5 TESTING HumaLyzer

5.1 Check voltage test point (Voltmeter 20 Vcc)

- Switch on the instruments

5.1.1 Check continuos Voltage (TP2 is ground).

- TP1  +9Vcc fixed

- TP4  -9Vcc fixed
- TP8  +5Vcc fixed

Check Voltage between the yellow wire and the black wire of J26 to measure about 13.5 Volt

5.2 Check linearity

- Switch on the instrument

- Select ABS
- Press “OK” key
- Extract flow-cell
- Position macro cuvette with distilled water in the cuvette hole, cover with black cylinder the head
- Press PUSH
- Press “0” key for zero setting
- Position cuvette with Dichromate at 25% in the cuvette hole, cover with black cylinder the head
- Press PUSH
- Annotate OPT TENSION value = Vbic25%
- Position cuvette with Dichromate at 100% in the cuvette hole, cover with black cloth the head
- Press PUSH
- Annotate OPT TENSION value = Vbic100%
- Vbic25% x 4  Vbic100% with range of  0,050 otherwise adjust trimmer RV1
- Test finish

6 OPTICAL GROUP

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6.1 Incubator

Incubator group contains:

- Incubator.
- One temperature sensor.
- Thermal resistor group.

6.2 Printer

Printer is a serial one with power supply at 5V, 3 A.

7 CONNECTONS CABLES WITH THE BOARD

J4-J3 Fans
J8 Lcd Power Supply
J15 Printer Power Supply
J16 Gnd earth
J24 Lamp
J26 Power Supply
J27 Preamplification Circuit
J28 Lcd board OR Programming cable
J31 Opb Sennsor
J32 Pump Motor
J33 Filter Motor
J36 Incubator heating elements and temperature sensor
J37 Peltier
J38 Peltier Temperature Sensor

7.1 Appendix 1: Changing lamp

This is the procedure to change lamp:

- Open case of the instrument, pulling out four screws down the equipment and six ones on the
back side.
- Unscrew the four screws around the cuvette site, and move away pump support (the one with
pump rotor).
- Remove the lamp protection box by unscrewing the two screws.
- Move away the little tongue that fixes the lamp, by pulling out the only screw.
- Extract old lamp, and set carefully the new one, bringing it up to the bottom of the site (pay attention to
not touch lamp glass with finger skin).
- Set up again the little tongue, and check the top of the lamp being inside the little hole.
- Perform Lamp Targeting (see pag.14)
- Set up again the lamp protection box
- Set up again pump support and equipment case.

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7.2 Appendix 3: Red tube changing

1. Disconnect the red tube from peristaltic pump by pulling the red holders.
2. Free the red tube from connector by pulling the two white connector out from red tube:
3. Insert a new tube

White Connector

Holders

7.3 Touchscreen Board:

Jp12 Jp11
External Ps2 Push + Serial Connection to main board

Jp9
Ext. serial

Jp10
Printer LCD
panel

Rv1
Jp6 trimmer
Power

LCD
BackLight

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8 MAIN BOARD

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8.1 Software and Firmware Upgrade

The HumaLyzer is composed by 2 boards, each board carries a microprocessor.

- The Touch Screen board carries a “Managing Firmware” of the instrument. This firmware can be loaded
directly from serial port present in the rear panel. The 99% of upgrades will be performed on this board.
- The main board controls all the peripherals (device), frequency of re-programming for the “Peripherals
Firmware” is rare. In order to upgrade the it is necessary to open the instrument.

8.1.1 Managing Firmware upgrade for HumaLyzer

In order to upgrade the instrument firmware, please follow step by step the bellow stated instruction:

1. Connect one side of the re-program cable to one of the available PC COM sockets and the other side to
the HumaLyzer RS232 sockets
2. Close the contact present on the re-program cable PC/instrument.
3. Switch on the instrument.
4. Start on PC the program FlashSta and perform the following operations:

Select the COM to which the re-program cable has been connected, then press OK

Press the button Refer

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 Select the file to load (demo.mot)

Press OK

Press Setting...

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 Select Baud rate 57600 and Program intervals (ms) 40 then press OK
The program will perform an automatic parameters settings (the operation will least few seconds)

Press E.P.R...

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 Press OK
Now the programming mode start, it will last some seconds and on the PC screen will appear the following:

The programming time is displayed by the blue bar; at the end of programming the blue bar will be complete.

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 When the programming is complete this message will appear.
Press OK then Exit in order to exit from the FlashSta program

NOTE: the exit from the program is not immediate, a delay of few seconds will be needed.

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8.1.2 Diagram wiring for re-programming cable:

Male Connector Female Connector

1 1
Green

White

Blue

Cable Switch

9 9

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8.1.3 Peripherals Firmware upgrade for HumaLyzer

In order to install the Peripherals Firmware on HumaLyzer, follow the procedure stated below.

8.1.4 Program microprocessor (for XP)

- Copy “flashprog16lx-v01l11.exe” on pc C:\Programs then install by double click.

- Search on your PC “ C:\ Programs\ FUJITSU\ FUJITSU FLASH MCU Programmer\ FMC16LX, and copy
“HM_002_13_Ott_2005.mhx ” file in this folder (remember to unzip first)
- Search on your PC “C:\ Programs\ FUJITSU\ FUJITSU FLASH MCU Programmer\ FMC16LX\Flash.exe and
open it by double click.
- Find on your PC, with “Open” buttom, the file “HM_002_13_Ott_2005.mhx” with which you want re-
program the instrument.
- You will see:

HM_002_13_Ott_2005.mhx

8.1.5 Automatic Software Loading

1. Select MB90F553A as CPU (Target Microcontroller)

2. Select 16MHz as speed (Crystal Frequency)
3. Select COM1 as PC port
4. Connect serial cable to PC and instrument (COM1), select from: Set Environment button
5. Open cover of HM and connect special adapter to J28 (see board), then connect serial cable to special
adapter.
6. Switch on Humalyzer
7. Press FULL OPERATION (D+E+B+P) button
8. A message will appear: “Press Reset Button”
9. Push Reset button on the main board press OK in message then wait for the end of the automatic
programming session, then press OK.
10. Switch off Humalyzer
11. Disconnect serial cable from the instrument
12. Switch on Screen Master and check software

In case of fail of the automatic software loading try the manual loading:

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8.1.6 Scheme Serial PC

FEMALE MALE

1 1
6 6

9 9
5 5

8.1.7 Special Adapter to J28

Serial female

AMP Mod. 4
Female

5 9

1 6

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8.2 Methods Parameter Download and Upload

In order to upgrade the instrument Assay parameters and make a backup of all the assay parameters present inside
the HM memory, please follow step by step the bellow stated instruction.

In order to operate these procedure the relevant program named SetupSmpDownload.exe must be installed in the
PC (the PC must be RS232 connection equipped)

- In order to install SetupSmpDownload.exe, copy it on desktop then bobble click and follow installation
indications
- After the installation is complete an icon will appear on desktop
- Click on icon and the following program will appear:

- Select Com by pressing Com button in case the PC has more than 1 com connection
- Select File, open the file and select the .Met(this is Human GmbH assay parameters adaptation) or other.

- In case no file is present in Select file option, go in C\HM\Method and copy the file

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8.2.1 Download Procedure

This program allows to load in the HM automatically methods parameters from a PC via RS232.

1) Connect one side of the re-program cable to one of the available PC COM sockets and the other side to
the HM RS232 sockets
2) Open the contact present on the re-program cable PC/instrument.
3) Switch on the instrument.
4) Start on PC the program SmpDownload.exe
5) Select File, open the file and select the.Met file, than press OK button
6) In the HM enter in service menu and select Download
7) In the SmpDownload program press Download button
8) The methods will be loaded into the Hm automatically

8.2.2 Upload Procedure

The same program allows to record the methods parameter stored inside a HM in the PC via RS232.

1) Connect one side of the re-program cable to one of the available PC COM sockets and the other side to
the HM RS232 sockets
2) Open the contact present on the re-program cable PC/instrument.
3) Switch on the instrument.
4) Start on PC the program SmpDownload.exe
5) Press File name button and name a file where all the assays parameters present on HM will be stored in
PC, then press Ok
6) In the HM enter in service menu and select Upload
7) In the SmpDownload program press Upload button
8) The methods of HM will be loaded into the PC automatically and stored in C\HM\Method

8.3 Procedure for Touch Screen Replacement

1. Switch off instrument, and disconnect power cord

2. To remove the cover: Unscrew the 4 screws located under the instrument bottom
3. Disconnect the plastic cover from instrument, to help operation open printer door, insert your finger
under the cover and lift.
4. Remove the 4 screws of the broken Touch screen by disconnecting JP8 and JP4 from Touch Screen Board.
5. Connect JP8 ( no polarity of connection needed) and JP4 (the blue colour on flat must be looking outside
board) of new Touch Screen.
6. Lock the Touch Screen with 4 screws

DANGER: WHEN THE INSTRUMENT IS SWITCHED

ON PAY ATTENTION OF CONNECTION JP8, HIGH TENSION PRESENT 220V

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 To close press both edge

To open lift both edge

JP4 connector

Touch Screen

Insert the blue flat cable

coming from the touch into Flat Cable
the connector on the
screen board
Touch Screen Board

JP4 JP8

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 Flat Cable

JP4

7. Connect a standard keyboard on PS2 socket located on the back of instrument.

8. Switch on instrument and regulate contrast with RV1.
9. Digit on Key board: “SRV”, the service menu will appear
10. Move down with keyboard arrows and highlight CALIBRATION TS, then press ENTER
11. Press F9 to start calibrate.

NOTE: DO NOT PRESS TEST BUTTON FOR ANY REASON!

12. with the tip of pencil touch the middle of the flashing indicator until it disappear
13. Repeat this operation for all the indicator that will appear in sequence (16)
14. Exit and the tip of the pencil must be in the same position of the indicator, try different position of
touch, especially the one where you report the problem on keyboard.
15. If touch screen is now calibrated press SAVE button or F10 (with external keyboard)
16. If still touch screen is still needing adjustment repeat operation from point 1

8.3.1 Check Calibration

Inside menu Touch with a pencil and an indicator will appear in correspondence of the pencil tip.

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The tip of the pencil must be in the same position of the indicator, try different position of touch, especially the one
where you report the problem on keyboard.
In case of not correspondence between pencil tip and arrow, repeat calibration procedure.

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HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
| Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
| Tel.: +49 61 22/99 88-0 · Fax: +49 61 22/99 88-100
| e-Mail: human@human.de · www.human.de