TKN/KS/16 – 5928 OR

Sixth Semester B. Sc. Examination Discuss in detail the structure and working of pBR 322.
10
BIOCHEMISTRY
Paper - II 4. Describe in detail the genomic and cDNA libraries.
10
(Molecular Biology and rDNA Technology)
OR
Time : Three Hours ] [ Max. Marks : 50
Write notes on :—
N. B. : All questions are compulsory and carry equal (a) Calcium–phosphate precipitation method of
marks. transformation. 5
1. Discuss in detail the experiments conducted for (b) Blue–White screening. 5
deciphering the genetic code. 10
OR 5. Sovle any 10 of the following :—
Write brief notes on :— (i) State the first codon that was deciphered and the
(a) Error correction in amino acylation. 5 amino acid that it codes for. 1
(b) Shine and Dalgarno sequence. 5 (ii) State any two characteristics of the genetic code.
1

2. With the help of suitable diagrams discuss in detail the (iii) Amino acid is attached to which end of tRNA?
role of : EF–Tu, EF–Ts, and EF–G in protein 1
biosynthesis. 10 (iv) Which subunit of the ribosome has the enzyme to
synthesize the peptide bond ? 1
OR
(v) Which three codons indicate stop signal for protein
Write brief notes on :— biosynthesis ? 1
(a) The roles of release factors RF1 and RF2. 5
(vi) In which of the two sites in the ribosome is the
(b) Formation of 70s initiation complex. 5 initiating codon lodged ? 1
(vii)What is the meaning of the term 'Shuttle vector"?
3. Write an essay on restriction enzymes. 1

TKN/KS/16 – 5928 Contd. TKN/KS/16 – 5928 2 Contd.

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 (viii) Which of the two DNA ligases, E.coli DNA
ligase or T4 DNA ligase, is normally used in
recombinant DNA technology ? 1
(ix) Give one difference between linkers and adaptors.
1
(x) Which enzyme is generally used for DNA
polymerization in PCR process ? 1
(xi) Give one major application of southern blotting.
1
(xii) What is meant by 'Bt' in Bt–cotton ? 1

TKN/KS/16 – 5928 3 750

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