Professional Documents
Culture Documents
Lecture 03 PDF
Lecture 03 PDF
Intermembrane space
Inner membrane
CO Q Complex IV
Complex 1 Complex III Complex V
FMN Cyt b Cyt c Cyt a
FeS FeS Cyt a3
Cyt c1
Complex II
FAD
FeS
In addition to these four enzyme complexes, their is a fifth complex (complex V) which is
the ATP synthase that is responsible for biosynthesis of ATP from ADP and inorganic
phosphate.
Sequence of events in the electron transport chain
The following diagram shows the sequence of events that occurs in the electron transport
chain
Isocitrate Succinate
Malate Acyl CoA
β-hydroxy acyl CoA Choline
β-hydroxy butyrate
Flavoprotein (FAD)
FeS
1/2 O2
The hydrogen atoms produced from oxidation of substrates can enter the chain through
+
FAD or NAD . The hydrogen atoms are then successively transferred through the
respiratory chain to oxygen to produce water and energy.
NAD+ collects the reducing equivalents from substrates as isocitrate, malate, β-hydroxy
acyl CoA and β-hydroxy butyrate, while FAD collects the reducing equivalents from
substrates as succinate, acyl CoA and choline.
+
The initial oxidation of NADH+H is catalyzed by a membrane bound NADH
dehydrogenase (complex I). The electrons are then passed to coenzyme Q.
4
Electrons from FADH2 are passed to coenzyme Q by enzyme complex II.
Ubiquinol (reduced coenzyme Q) is oxidized by ubiquinol dehydrogenase (Complex III).
The 2 hydrogen atoms are removed from ubiquinol but they cannot be transferred to
cytochrome b as cytochromes can accept or transfer only electrons. So at this step the two
hydrogen atoms liberated from coenzyme Q will be ionized giving 2 hydrogen ions and 2
electrons. The hydrogen ions will be liberated into the mitochondrial matrix and the 2
electrons will then reduce the iron in cyt b.
The electrons will be successively transferred to cyt c1, cyt c, cyt a and cyt a3
--
Lastly, electrons are transferred to oxygen by cytochrome oxidase and ionic oxygen (O )
will be produced.
Being negatively charged, ionic oxygen attracts 2 hydrogen ions from the mitochondrial
matrix to form water.
N AD lin ke d
d e h yd ro g e n a s e s
+ re d u c e d O x id ize d red u c e d O x id iz e d
A NADH+H FMN
c o e n zym e Q c yt b (2 F e + 3 ) c yt c 1 (2 F e + 2 ) cyt c (2 F e + 3 )
2H+
red u c e d O x id ize d re d u ce d 1 /2 O 2
cyt c (2 F e + 2 ) c yt a (2 F e + 3 ) c yt a 3 (2 F e + 2 )
S e q u e n c e o f e ve n ts in th e e le c tro n tra n s p o rt c h a in