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Of Acid Of: Dinitrosalicylic Determination Sugar
Of Acid Of: Dinitrosalicylic Determination Sugar
Bismuth. The chunk metal (Fisher Sci- sium brómate using the Fisher Titrim- brómate method, vdiich requires the
entific Co.) was crushed and the coarse
eter with a platinum-calomel electrode addition of hydrochloric acid.
pieces were sorted out.
Silver. Granular silver (G. Frederick pair. Other reductors studied were lead,
Blanks were run on the reductors
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Rochelle salt, normally present in DINITROSALICYLIC ACID REAGENT, The major defect in the test is in the
the dinitrosalicylic acid reagent for THE
developed by Sumner and co- loss of part of the reducing sugar being
reducing sugar, interferes with the worker (11-14) for the determination analyzed. This was pointed out by
protective action of the sulfite, but is of reducing sugar, is composed of di- Sumner (12, 14), was referred to by
essential to color stability. The diffi- nitrosalicylic acid, Rochelle salt, phenol, Brodersen and Ricketts (2), and has
culty may be resolved either by sodium bisulfite, and sodium hydroxide. been observed repeatedly in this lab-
eliminating Rochelle salt from the rea- According to the authors of the test, oratory (6, 8, 9). Evidence of loss of
gent and adding it to the mixture of Rochelle salt is introduced to prevent sugar is also given by the data of Hos-
reducing sugar and reagent after the the reagent from dissolving oxygen (12); tettler, Borel, and Deuel (4) and of
color is developed, or by adding phenol, to increase the amount of color Bell, Manners, and Palmer (1). As
known amounts of glucose to the produced and to balance the effect of this defect appears never to have been
samples of reducing sugar to com- phenol encountered in urine (IS)·, and fully remedied, the present study was
pensate for the losses sustained in the bisulfite, to stabilize the color obtained carried out to investigate the different
presence of the Rochelle salt. The in the presence of the phenol (IS). factors which might cause it. In the
optimal composition of a modified The alkali is required for the reducing course of the investigation, the effects
dinitrosalicylic acid reagent is given. action of glucose on dinitrosalicylic acid. of varying the concentrations of the