I acknowledge the many teaching assistants who helped in developing some
of these exercises: Daniel Caulkins, Cheryl Knox, John Finer, Richard Norris, Ann Reilley-Panella, Ricardo Diquez, Eugenio Ulian, Shelly Gore, Sara Perez- Ramos, Jeffery Callin, Greg Peterson, Sunghun Park, Maria Salas, Metinee Srivantanakul, and Cecilia Zapata. Additionally, all the students who have taken this course since 1979 have been instrumental in developing and improving these exercises. The contributions of Dr. Trevor Thorpe with the chapter on the history of plant cell culture, Dr. Brent McCown with a chapter on woody trees and shrubs, and Dr. Sunghun Park, Jungeun Kim Park, James E. Craven, and Qingyu Wu with the chapters on protoplast isolation and fusion and Agrobacterium- mediated transformation are tremendously appreciated. Last, I thank my husband, Jim, daughter, Cristine, and son, Will, their spouses, Gaylon and Trudy, and my grandchildren, Claire Jean, William, Clayton, Wyatt, and Grant. Especially the grandchildren for taking their naps while I had high speed internet at their homes to access library databases to update this edition.