Technical Evaluation: (For all technicians) March 2019.

Phlebotomy and Sample collection:

1. Name the anticoagulant used for collecting blood sample for PT INR ____________.
2. What gauge needle is used to draw blood sample in SIGMA hospital?
3. Which color capped tube is used for collecting blood to do electrolytes?
4. Which color capped tube is recommended for collecting blood for glucose test?
5. What is the volume of blood that can be collected in Microtainer?

Biochemistry:

1. How many levels of quality control do we do in SIGMA lab? What are the names of the
quality controls?
2. Calculate VLDL and Chol/HDL in the following patient:
Cholesterol: 256 mg/dl; HDL: 43 mg/dl, Triglycerides: 158mg/dl.
3. What is critical alert policy of SIGMA hospital for biochem lab results? Give any one
example of the test name and the value at which alert is given.
4. When is calibration done for the EM 200 machine calibrated for glucose test? Give
atleast two situations.
5. What are the units for Serum Sodium in electrolytes?

Serology:

1. Write the full form of HCV test.

2. Interpret the result for HIV test:

3. How much sample volume is needed for doing one test on Minividas?
4. What are the tests that are performed on MiniVidas in our laboratory? What is the test
method called?
5. What are the four antigens used in WIDAL test? What is the titre for Tube WIDAL test,
when it is reported as positive?
Microbiology:

1. What is the name of the media used for doing Antibiotic sensitivity test in our lab?
2. What is the reagent used to detect Indole production?
3. Name two organisms that are gram positive.
4. Name any five antibiotics used in antibiotic sensitivity in Microbiology.
5. Which is the agar media used to detect lactose fermentation?

Hematology:

1. What method of blood grouping is followed in SIGMA lab?

2. What is the color of Coomb’s reagent?
3. What is the reagent used for doing reticulocyte count?
4. What is the full name of the anticoagulant used to collect blood sample for CBC?
5. What are the types of white blood cells that can be seen on peripheral smear?

Clinical Pathology and general duties in lab:

1. Name two organisms that can be seen on stool microscopy.

2. How is waste segregated in the laboratory? Write the color code of the bin in which the
following is discarded:
3. What is the documentation procedure for Histopathology tests?
4. What is the documentation procedure for outsourced investigations?
5. Write down all the steps that are done for routine investigations, when an Inpatient
sample comes to the lab?
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Additional questions For Srinivas, Kavya and Adam

1. Describe the tests done in Semen Analysis.

2. Describe the steps in Pap staining.
3. Describe procedure for doing direct and indirect coomb’s test.
4. What is the procedure for quality control in our laboratory?
5. Describe the steps followed for performing blood culture test after sample is received in
the lab.
6. What is the Critical alert policy in SIGMA lab? Give two examples.
7. Describe the method will you follow to do CBC in a baby when only two drops of EDTA
blood is available?
8. What is the SOP to be followed if a blood sample is spilled on the floor?
9. When is calibration done on EM200? Give two situations.
10. Name atleast four tests that are done in SIGMA lab for identifying biochemical
characteristics of an organism.