ABSTRACT

THE EFFECT OF PEANUTSHELL EXTRACT (ARACHIS HYPOGAEA) TO

TRIGLYCERIDE LEVEL IN RATS (RATTUS NORVEGICUS) INDUCED BY HIGH FAT
DIET

Tannia Rizkyka Irawan1, Joko Anggoro2, Seto Priyambodo 2

1Medical Doctor

2Lecturer of Medical Faculty of Mataram University

Background : Coronary heart disease is the most common cause of death in Indonesia. As a
way to prevent cardiovascular disease, peanut shell has been develop to be an alternative
way. The present bioactive compound, luteolin, acts as antioxidant and can also influence
trygliceride metabolism. The purpose of this study is to find out the effect of the peanut shell
extract (Arachis hypogaea) on triglyceride level in rats (rattus norvegicus) induced by high
fat diet .

Method : Present research is an experimental research with post test control group
only. The animal trial that used were 18-20 weeks, 200-250 gram, male, wistar rats (Rattus
norvegicus). The rats divided into 5 groups. They are consist of negative control group
induced by daily diet, positive control induced by simvastatin 0,9 mg/kgBB/daily, and three
treatment groups induced by three different dosage of peanutshell extract (Arachis
hypogaea). Three different dosage consist of 300mg/kgBB/daily (P1), 600 mg/kgBB/daily
(P2), and 1200/kgBB/daily (P3) orally. Before the treatment start, we induced the rats with
high fat diet 10mg/daily for 6 weeks to make hypertrygliceride state. The treatment is given
for 19 days. The source of the data is taken after the treatment, by taking the blood from
intacardiac, which had been fasted for 12 hours. The level of triglyceride checked from all
groups, then the result had been analyzed with ANOVA.

Result :There is significant difference (p=0,01) between treatment and control group.
They are between KN and P3 (p= 0,043), KP and P2 (p=0,03), P2 and P3 (p=0,02).

Conclusion :Based on the result, it can be concluded that the oral induction of
1200mg/kgBB/daily nutshell extract (Arachis hypogaea) for 19 days on rats, have a
significant effect on lowering the level of triglyceride (p<0,05).

Keywords: peanutshell extract (Arachis hypogaea), high fat diet, triglyceride, Rattus
norvegicus.