(CORE BIOLOGY PRACTICALS, You wil need to know these practcals as the exam board may ask You questions based on them. Below is @ summary of each one ame of praca and | Other variables to | Other equipment ‘Mathod and outeome independent & dependent be controled variables tet 7 Tenpecrtire ineeape Sater Wathod: rio Dania sr placeyn cay ae eve pond vata + nang Dap were ame se ‘fect ofeateve on Daphnia | Volume of scavons | cavtyside sopping | replace wih dated water Lever enbsane court | iTlstaolong undermirasope, temp Teor rot { stearaf Daphnia | Bees stopcock, | heart rte under meroscope ore mule ror 20 le exon increases auetalong}=incresedbeartte_| 4 sreot Caphnia,oy, | sted water est | Repeat wh 2 more Ozpria. ReBeEga thine with smaliconcerttonof | « aurgenoush dla colcted Independent cattene | Stimeoh | “tubes stopcock | eaten lution pac of tied water Cory autforsconantatonsofcaeine | Toniighensceniaton fae its toncenaten 2 repeat at 3careptavons. cane Dependent arta ‘teome: flee concentration inert, heat rte neon eee erie, Srvata Darina | Tepes IH DOR stan, Te io er abe oamgearninemmeeamianeiwl. | > measuring ve content | conceneaten of [V rp. hak tube gent aterenensrap Cantu | + min in feat pee ere 5: Record vote af sation needed fo Independent rl + Shake each tube same number of + sameend pont colour unt ne beakers pipette reqvewd to gecolovriee tem OCPIP sin to deeoourse Lem’ of DCP. ‘Mass insompie= mass of vitamin Ct decelourse lem DCP ~ volume of sample Pani ecloures + Some oe of soliton when trans ‘ona baker to anther 1 Accuracy of measuring equoment The effect temperature on el ‘membranes Independent: temperature of stop dock dstiles water singe ‘ach and leave for 30 mins Remove betion an Ske ti ‘oorimeter to absorbance on blue /ree {Coleultions & outcome: to clelate transmission» 200 | as terperature creased vansmision treaty increased due to membrane molecules ‘more toa point where Felease of dye als proteins in membrane danature sn rations caused lage gaps nthe membre ena bats of diferent temperature. Once st temperature, ad plese of beta to esto sparse ye, Set, + alirate wing stile water ins ‘aves st ther a8 2em3 o betrat slution from the fest tempo rer eet: Place nto colorimeter read abeorbanc. Repent ora other pce ‘habeorance. Ensuring sme amount of tine athe ferns temperate cab battanel ig dlysioult ‘concentration oncote of Independent concentration of dependent time taken mayne to bres down subst Temperature Volume of ensyme Volume of| suerte Concentration of| suerte pM ‘Method: make un diferent canentatons of nsyme wi siterent it water bat longi second be contining Allow te aeclmatse for 3 mins so that tame tomperstre Colultions & outcome: rate = 1ime | As concentration ef enn rans er dierent chemist provent eras conta temporare to oan constant Pat 1 tst ube of Same Repeat 3 Dem’ of 0.2% try thon aa “asin srt stop cock Time how long takes for esa saluton totum Waneparent [mark 2% onthe other seo ube 35 Soon as seen through selutin top clo Repeat further 2 tes thon rept for nest concentration, tise to dened fo. Set up eas syringe and ie, Read off the woume of a fae of action = radent at steepest point uteome as protease | > Atiachig srnge ean be slower slowing loss "0 soe the cos though the solution 00 qucty to read[Name of practical and ‘Other equipment ‘Method and outcome Possible evaluation lasues Independent & dependent variables Method Tube of end IM HeTnta FC waterbath / observing titois Cromosomes stained blue using ‘resin ethanol @ 60°C 2 watch stsses, test tube, 2 pipetes, microscope slides, forceps, meunted noedle fiter paper, rmeoszope wth mag 100 x00. Irom gareroot. Put mins. femove then place water, Leave for 45 mins then repeat process fraple atts poi. Transler 2 tp ta microscope aide, cut -Srm from growing ip (ste of mits) an keep the xp. Gert breakup (macerate) with mounted needle, ‘1 smal drop of rein ethanol stain ad leave fo 2 mins. Ad eaves ar Blot +r paper, ew Under microscope afin the tages of moss ons: percentage of calm each stone mitoss Totipoteney 8 Tasue Culture BaMe a same te Tests of white mustard, weighing sels, ste Push the tem of the export nto the gel faking sure cotyledons de’ t touch ag] ty 250r Beker, ga | cover wth cing fn an lesan sony windows Obeens oer 10 dy. eA red ececrcsey | Cocco ole er an tee gee ne ston dkny ‘window si ceapsin why the ae covered incl fir and why they continue t grow even when eon ume, Covered Auouy they shel’ be opened a3 > Length offs, €5R Imesvenatnofplacsores | + Secteach indaioimass | _papertovel chimp Independent: source andtpeat | sands ted mses fre * ftom Aine game] nd resto Dependent masetht ante | plainly, game a rad pl Can Pa + Rone gatetn > Velume amines Le aera Invertigting lent incre! |” soliton buble aught neo eran cdeienies + Spec of pnt : ierorganém growth nner characteris of Ue pnt + Suef container + amount of ight receive Inaiicent time tose an effect Lrg adlenaies, fect of gore ond mint on / yecteril qrowth Independent presence of atc Denendent zone ‘round lc TUM | V7 Ba eerie, sects = concentration of ‘sh + contamination of pet ash by ater bazar tae, marker pen fate and spt into sections, for each type of pant extract. Place 1 seo och fetract in each quadrant ofthe aga slate close ad tage nth hazrd tape, Lee to Incubate over right and observ zone of inhi ‘alstled watecon discs. ‘outcome: treat large ones of hi lower conantratons. n eamyouceonrast st covered on than others, meaning thay ae moreeffecve at ted ierobes on ag ates de to bad asetitecrniques, Not shaking exact encughto ensure enough active ingredient Inconsistency wen seg plant etre to Jeng wrong spe of nator forlawn »‘A2 BIOLOGY CORE PRACTICAL SUMMARY ane pasa Hepat | otervanier | sipman Jide atte Sai ener cos a pe a ee ee ee Se | Sarees [see rn eee tas eee eee eee (| See ee | ee ee pune ae Sn ee ee ee oer a ae eae! Saget caro | ee ee ee Fs saa fmcmrowonte |> treme | + Staonteracn | ailetrct resin puto iene Saatesie Maat |< castor eyaraun eet eect Pare tang nero onoe aera ctis ed Sete eet ieee, |; cee: Settee |* tomes |. Sitmnawnar | tr hetepee east ee ae ce eee ot eerie | secemyreera Seen ae Sea inrcitoa ucatromicam aes en ERC: |, cere ns — Rec! Probbirerbernmiernstscitempetetiboe recy yh lacie eS ai al cere See eanan wee cae ae eee ney A etre | tet eee eee at ocean peel aes selon scab omer S| a ea ee La ental a rae ‘DNA emplification > Thermacycler ‘DNA sample is placed into tube in thermocyéler with nucleotides, primers and polymerase. Step 1: denatur ‘DNA heate a eae | Se cc ae a | See eee