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Activity 11 Discussion Serum Plasma Preparation Centrifuging and Transferring
Activity 11 Discussion Serum Plasma Preparation Centrifuging and Transferring
Activity 11 Discussion Serum Plasma Preparation Centrifuging and Transferring
SERUM AND PLASMA • Apply the proper method on serum and plasma preparation.
PREPARATION • Compare and contrast the difference between serum and
DEPARTMENT OF MEDICAL TECHNOLOGY plasma.
FACULTY OF PHARMACY
• Discuss the common causes of unacceptable serum or plasma
specimens and inaccurate results.
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BLOOD FUNCTION
> red fluid that circulates inside blood vessels
> Transports nutrients, waste products, hormones, protein, ions ,
> Consists of formed elements (erythrocytes, leukocytes, and
Oxygen, CO2 and formed elements
platelets) and a fluid component called plasma
> Regulates body temperature
> Volume of blood in an average human adult is 5 L.
> Assists in the regulation of osmotic and acid-base balance
> Circulates in a closed system of vessels
> Protection (Immune Defense)
> Have short life spans and are continuously replaced by
> Prevents excessive blood loss (Clot Formation)
hemopoiesis
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SERUM PLASMA
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• is a clear, straw-colored liquid portion of the blood in • The blood is mixed with an appropriate amount of
which the other cells are suspended. anticoagulant
• transporting medium for cells and a variety of substances • This preparation should be mixed immediately and thoroughly
to avoid clotting.
vital to the human body.
• It is then centrifuged for 15 minutes at 2000 -2500 rpm.
• The supernatant fluid is then separated and then labeled
Plasma = water + proteins + dissolved substances properly
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PLASMA PROTEINS
SERUM
• The clear liquid that can be separated from clotted blood
Albumin Fibrinogen • is about 90% water with dissolved proteins, minerals, hormones and
60% 4% carbon dioxide and is an important source of electrolytes
• In blood, the serum is the component that is neither a blood cell nor a
Other 1% clotting factor; it is the blood plasma with the fibrinogens removed i.e.
Globulin of blood
35% protein
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content Serum = Plasma - Clotting factors
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