Time Log 

DATE  TIME  TASK/DESCRIPTION 

Monday  9am-4pm  ● Training/Confidentiality paperwork 

June 11, 2018  (7 hours)  ● Lab Tour 
● Researched/read up on project 

Tuesday  7:30am- 4pm  ● Online training 

June 12, 2018  (8.5 hours)  ● Made media and put cells onto 
coverslips (Project 1) 

Thursday  8am-4pm  ● Researched 

June 14, 2018  (8 hours)  ● Washed, aspirated, and fed cells (kc) 
● Started other cells (mc) used other 
media 

Monday  8am-4pm  ● Rinsed cells  

June 18, 2018  (8 hours)  ● Put buffer on to attach to the proteins 
and sat overnight 

Tuesday  12pm-4pm  ● Rinsed cells 3x 

June 19, 2018  (4 hours)  ● Put fluro buffer onto cells 

Monday  8am-4pm  ● Started 3 cell lines 

June 25, 2018  (8 hours) 

Tuesday  7:30am-2pm  ● Fed cells 

June 26, 2018  (6.5 hours)  ● Fixed 1 plate of pig keratinocytes 

Thursday  7:30am-4pm  ● Part A into 6 well plates 

June 28,2018  (8.5 hours) 

Monday  8am-4pm  ● Cells onto coverslips 

July 2, 2018  (8 hours) 

Thursday  8:30am-4pm  ● Fed cells 

July 5, 2018  (7.5 hours)  ● Started new MSC’s (others died) 

Monday  8:30am-4pm  ● P2 HEK- 6 well plate into incucyte 

July 9, 2018  (7.5 hours)  ● P3 MSC- 6 well plate into incucyte 
● Both put in the media they like 

Tuesday   2pm-4:30pm  ● Fed and split HEK cells and MSC cells 
July 10, 2018  (2.5 hours)  ● Continuing to grow the cells 
Thursday  8am-4pm  ● Fed Cells 
July 11, 2018  (8 hours)  ● Changed media in 6 well plates 
● Used 3 different medias 

Monday  8:30am-11am  ● Changed media in 6 well plates for both 

July 16, 2018  (2.5 hours)  HEK and MSC back to just media 1. 
● Let HEK in HEK media keep growing until 
they are ready to stain 

Tuesday  8:30am-4:30pm  ● Changed media in HEK cells (6 well 

July 17, 2018  (8 hours)  plate) 
● Threw away HEK cells in MSC media 
(They did not like that media and died) 
● Letting HEK cells grow  

Thursday  8am-4pm  ● Stain cells 

July 19, 2018  (8 hours) 

Tuesday  8am-4pm  ● Data/ Charts/ Graphs 

July 24, 2018  (8 hours) 

 
Total Hours: 118.5