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    Faculuad de Cienciau Nauuualeu Niuelaciun U in Enieuua Ambienual

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    Roxy Estefania Monserrate
    This document summarizes a student project on amylase enzymes. The project involved isolating microorganisms that produce amylases, extracting the enzyme complexes, and measuring amylase activity through an enzymatic reaction. Testing of the complex extracted from an Aspergillus sp. fungus grown on wheat bran showed amylase activity equivalent to a dextrose reading of 6.5 to 6.53 on the enzyme assay. The study successfully isolated microbes that produce amylase enzymes and measured their activity.

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    Faculuad de Cienciau Nauuualeu Niuelaciun U in Enieuua Ambienual

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    Roxy Estefania Monserrate
    14 views12 pages
    This document summarizes a student project on amylase enzymes. The project involved isolating microorganisms that produce amylases, extracting the enzyme complexes, and measuring amylase activity through an enzymatic reaction. Testing of the complex extracted from an Aspergillus sp. fungus grown on wheat bran showed amylase activity equivalent to a dextrose reading of 6.5 to 6.53 on the enzyme assay. The study successfully isolated microbes that produce amylase enzymes and measured their activity.

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    Amilasas

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    This document summarizes a student project on amylase enzymes. The project involved isolating microorganisms that produce amylases, extracting the enzyme complexes, and measuring amylase activity through an enzymatic reaction. Testing of the complex extracted from an Aspergillus sp. fungus grown on wheat bran showed amylase activity equivalent to a dextrose reading of 6.5 to 6.53 on the enzyme assay. The study successfully isolated microbes that produce amylase enzymes and measured their activity.

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    14 views12 pages

    Faculuad de Cienciau Nauuualeu Niuelaciun U in Enieuua Ambienual

    Uploaded by

    Roxy Estefania Monserrate
    This document summarizes a student project on amylase enzymes. The project involved isolating microorganisms that produce amylases, extracting the enzyme complexes, and measuring amylase activity through an enzymatic reaction. Testing of the complex extracted from an Aspergillus sp. fungus grown on wheat bran showed amylase activity equivalent to a dextrose reading of 6.5 to 6.53 on the enzyme assay. The study successfully isolated microbes that produce amylase enzymes and measured their activity.

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    of 12

    FACULUAD DE CIENCIAU NAUUUALEU

    NIUELACIUN U INENIEUUA
    AMBIENUAL
    TEMA
    Amilasas
    INTEGRANTES
    Arelis Balon
    Jahaira Cañola
    Roxana Monserrate
    Carla Villacis
    UEUUMEN
    LU UUUUUUUUUUU UU UUUUUUUU UUUUUUUU UUUUUUUU UUUUUUUU
    UUUUUUUUUUUU UUUUUUUUUUUUUUU UUUU UUUUUUUU UUUUUUUUUUUUU
    UU UUUU UU UUUUUUUUU U U UU UUUUUUUUUU UU
    UUUUUUUUUUUUUUU. EU UUUUUUUU UUU UUUUUUU UUU UUUUUU
    UUUUUUUUUUUUUUU UUUUUUUUUUU UU UUUUUUUU UUUU UUUUUUUU
    UUUU UUUU UU UUUUUUU. LUU UUUUU UUUUUUUUUUU UU UUUUUUU
    UUUUUUUUUUUU UUU UUUUUUU UU UUUUUUUUUU U UUU 18 UUUU
    UUUUUUUU UUUUUUUUU UU LUUUU (I2KI=0.026%I2+0.26%KI). LUU
    UUUUU UUUUUU UUUUUUUU U UUUUUUUUUU UUUUU UUUUUUU UU UUUUU
    UUUU UU UUUUUUUUUU UU UUUUUUUU (AUUUUUUUUUU UU.). LU
    UUUUUUUUU UUUUUUUUU UU UUUUUUUUU UUUUUUUUUU UUUUUUU UUUU
    UUUUUUUU U 37UC UUUUUUU 10 UUUUUUU U UU UUUUUUUUUU
    UUUUUUUU UUUUUUUU UUUUUUUUUU. EU UUUUUUUUUUU UU
    UUUUUUUU (DE) UUU UU 6,5, UU UUUU UUUUUUU UU UUUUUUUUU
    UUUUUUUUUU.
    •
    INUUDUCCIUN
    EU UUU UUUUUUUUUU UUU UUUUUUUU UU UUUUUUUUUUUUU U UUU
    UUUUUUU UU UUUUUUUUU UUUUUUUUUU UUUUUU UU UU UUUUU,
    UUUUUUUU UU UUUUUUUUUUU UUUUUUUUUUUUUU UUUUUUUUU UUU
    UUUUUUUU UU UUUUUUUUUUU U UU UUUUUUUUU UU UUUUUUUUUUUUUUU
    UU UUUUUUU UUUUUUUUUU (DUUUU, 1998). LUU UUUUUUUU UUU
    UUUUUUU UUU UUUUUUUUU UUU UUUUUUUUUU UUUUUUUUUUUU UUU
    UUUUUUU, UUUUU UUUUUU U UUUUUUUU UUUUUUUUU UUU UUUUUU UUU
    UUUU UUUUUUUUUU UUUU UUUUUUU UUUUUUUUUUU U UUUUUUUU
    UUUUUUUUUUUU. LUU UUUUUUUU UUU UUUUUUUUUU UUUUUUUU UU
    UUUUUUUU U UU UUUUUUU; UUU UUUUUUU, UUUU UUUUU
    UUUUUUUUUUUU, UUU UUU UUUUUUUUUU UUU UUUUUUUU UU UUUUUU
    UUUUUUUUUU U UUUUUUU., 2000).
    • LUU -UUUUUUUU UUUUUUUUU UUU UUUU UUUUUUUU UUUUU UUUU UU
    UUUUUUUUU UUUUUU UUUU UUUU UU UU UUUUUUUUU UUUU UUUUUU
    UU UUUUUUUU UUU UUUUUUU UU UUUUUUUUUU UUU UUUUUU
    (UUUUUUUUU). AUUUUU UUUUUUUUUUUU UUUUU UUU UUUUUUU U-1,4
    UU UU UUUUUUUU, UUUUU UUUUUU U UUUUUUUUU UUUU UU
    UUUUUUUUUUU, UUUUUUUUUUUUU, UUUUUUUUUUUUU U UUUUUUUUUUUU,
    UUUUU UUUUU (FUUUUUU, 2000).
    • UU UUU UUUUUUUUU UUUUUUUU UUUU UU UUUUUUUUU UU UUUUUUU
    UUUUUUUUUUU. LU UUUUUUUUUU UU UUUUUU UUUUUUU UU UU UUUUU
    U UUUUUU UU BUUUUUUU UU. AUU UU UUUUUU UUUUUUUU
    UUUUUUUUUUUUUUU UUUUUUUUUUU UUU UUUUUUUU UUUUUUU
    UUUUUUUUUUUU UUUU UUUUUUUUU UUU UUUUUUUU UUUUUUUUUUUU
    (DUUUU, 1998; FUUUUUU, 2000).
    MEUDLUA
    Medición
    actividad
    Extracción del enzimática
    complejo
    Aislamiento de enzimático
    microorganismos
    productores de
    amilasas
    UEUULUADU U
    DIUCUUIUN
    Figura 1. Aislamiento de microorganismos productores de
    amilasas a) y b) microorganismo 1, no hubo reacción de almidón
    con el lugol, c) y d) microorganismo 2, se puede observar
    almidón presente por la reacción con lugol.
    A B

    C D
    Tabla 1. Resultados
    actividad amilasa del
    complejo enzimático derivado
    del cultivo de Aspergillus sp.
    sobre salvado de trigo.
    Volumen Equivalente
    complejo Concentración glucosa de dextrosa
    Ensayo enzimático (g/mL suspensión de (DE)
    (mL) almidón)
    Blanco 0 0 0
    1 10 3,262E-5 6.50

    2 10 3,26E-5 6.53
    CNCLUUINEU
    UU UUUUUUUUUU UUUUUUUU UUUUUUUUUU UUUU UU
    UUUUUUUUUUU UU UUUUUUUUUUUUUUU UUUUUUUUUUU UU
    UUUUUUUU UUUUUUUUU UU UUUUUUUU UU UUUUUUUU.
    AUUUUU UU UUUUU UUUUUU UUU UUUUUUUUUUUUUUU, UU
    UUUUUU 1, UUUUUUUUUUUU UUUU AUUUUUUUUUU UU.
    UUUUUUUU UUUUU UUUUUUUUU UUUUUUUUUUU UUU UUUUUUU,
    UUUUUUUUUU UU UUUUU UUUUUUUUUUU UUUUUUUU UU
    UUUUUUUU UU UUUUUUUU UUUU UU UUUUUUU UU UUUUUUUUU
    UUUUU. LU UUUUUUUUU UUUUUUUUUUU UUU
    UUUUUUUU UUUUUUUUUU UUU UUUUUUUUUUUU
    UUUU.

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