Professional Documents
Culture Documents
Method in Microbiology
Method in Microbiology
Sc
Department of Food Science and Technology
• General purpose fortified by • favor the growth of • Media that use for distinguish
special nutrient to encourage particular microorganisms between different group of
the fastidious heterotroph • For isolation specific bacteria
• Ex: Blood agar mikroorganism • permit tentative identification
• Ex: Endo agar, eosin of microorganisms based on
methylene blue agar, and their biological
MacConkey agar characteristics
• MacConkey agar is both
• differential and selective.
contains lactose and neutral
red dye, lactose-fermenting
colonies appear pink to red
in color and are easily
distinguished from colonies of
non fermenters.
CULTURING MICROBES
The Five “I’s
Inoculation: Producing a pure culture
Isolation: Colony on media, one kind of microbe, pure culture
Incubation: growing microbes under proper conditions
Inspection: Observation of characteristics (data)
Identification: use of data, correaltion, to ID organism to exact
species
CULTURING MICROBES
Innoculation: Producing a pure culture
Introduce bacteria into a growth medium using “aseptic technique” to prevent
contamination. Tools: Bunsen burner, loop. Needle, etc.
CULTURING MICROBES
Innoculation: Producing a
pure culture
Introduce bacteria into a
growth medium using
“aseptic technique” to
prevent contamination.
Tools: Bunsen burner, loop.
Needle, etc.
CULTURING MICROBES
Isolation: Colony on media, one kind of microbe, pure culture
CULTURING MICROBES
CULTURING MICROBES-ISOLATION
Blood agar : rich with nutrients, can see a difference, thus differential; much more
later
INCUBATION
Incubation: Allow organisms to grow
under the optimal conditions
Temperature, with or without oxygen
etc
INCUBATION