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Culturing The Cancer Cells
Culturing The Cancer Cells
Culturing The Cancer Cells
Make media
2) After you take out the cells from -80. C/liquid nitrogen, thaw
them in the water bath for a minute or less until it's liquid.
6) Add media to the plate or flask depending upon what you are
using for culturing the cancer cells.
For a 6cm plate, total volume of media needed = 5 ml
For a T25 flask, total volume of media needed = 10 ml
For a T75 flask, total volume of media needed = 23 ml
8) Incubate at 37. C
Passaging
2) Wash with PBS (7ml so that cells do not dry out and makes
trypsinizing process quicker)
10 cm plate. 3 ml
6 cm plate. 2 ml
35 mm plate 1 ml
8) After all the cells are detached, transfer the content to the tube
containing media volume ≥ added amount of Trypsin.
10) Aspirate
11) Add media to the cells