Comparison of Hamstring Muscle Activation

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ORIGINAL RESEARCH

IJSPT COMPARISON OF HAMSTRING MUSCLE ACTIVATION


DURING HIGH-SPEED RUNNING AND VARIOUS
HAMSTRING STRENGTHENING EXERCISES
Roland van den Tillaar1
Jens Asmund Brevik Solheim, MSc1
Jesper Bencke, PhD2

ABSTRACT
Purpose/Background: Several studies have examined the effect of hamstring strength exercises upon hamstring strains in
team sports that involve many sprints. However, there has been no cross comparison among muscle activation of these
hamstring training exercises with actual sprinting. Therefore, the aim of this study was to examine different hamstring
exercises and compare the muscle activity in the hamstring muscle group during various exercises with the muscular activ-
ity produced during maximal sprints.
Methods: Twelve male sports students (age 25 ± 6.2 years, 1.80 ± 7.1 m, body mass 81.1 ± 15.6 kg) participated in this study.
Surface EMG electrodes were placed on semimembranosus, semitendinosus and biceps femoris to measure muscle activity
during seven hamstrings exercises and sprinting together with 3D motion capture to establish at what hip and knee angles
maximal muscle activation (EMG) occurs. Maximal EMG activity during sprints for each muscle was used in order to express
each exercise as a percentage of max activation during sprinting.
Results: The main findings were that maximal EMG activity of the different hamstring exercises were on average between
40-65% (Semitendinosus), 18-40% (biceps femoris) and 40-75% (Semimembranosus) compared with the max EMG activity
in sprints, which were considered as 100%. The laying kick together with the Nordic hamstring exercises and its variations
had the highest muscle activations, while the cranes showed the lowest muscle activation (in all muscles) together with the
standing kick for the semimembranosus. In addition, angles at which the peak EMG activity of the hamstring muscle occurs
were similar for the Nordic hamstring exercises and different for the two crane exercises (hip angle), standing kick (hip
angle) and the laying kick (knee angle) compared with the sprint.
Conclusions: Nordic hamstring exercises with its variation together with the laying kick activates the hamstrings at high levels
and at angles similar to the joint angles at which peak hamstring activation occurs during sprinting, while cranes did not reach
high levels of hamstring activation compared with sprinting.
Level of Evidence: 1b
Key words: Electromyography, muscle activity, hamstring, sprint

CORRESPONDING AUTHOR
Roland van den Tillaar, PhD
Department of Sports Sciences and Physical
Education
Nord University
Odins veg 23, 7603 Levanger
1
Department of Sport Sciences and Physical Education, Nord Norway
University, Levanger, Norway Phone: +47-5767 1883
2
Human Movement Analysis Laboratory, Dept. of Orthopaedic
Surgery, Copenhagen University Hospital, Amager-Hvidovre, Fax: 0047-7411 2001
Denmark E-mail: roland.v.tillaar@nord.no

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 718
DOI: 10.16603/ijspt20170718
INTRODUCTION late the power developed by the hamstring during
Hamstring strain injuries are one of the most fre- the late swing phase of sprinting. However, none
quently occurring injuries in sports, representing of the existing training studies directly compared
approximately 12-24% of all athletic injuries.1-3 There the hamstring activity during the different strength
is a high prevalence of hamstring strain injuries in training exercises and the activity during the sprint
many sports, including soccer, 4,5 Australian football, phase, which makes it difficult to determine whether
6
American football7 and sprinting. High-speed run- the muscle training is specific enough for high speed
ning is a common denominator among these activi- running. Furthermore, it is not known if the angles at
ties and is the activity accounting for the majority of which the different exercises are conducted are simi-
hamstring strains.8 lar to the position of the limb during late swing phase
and thereby the hamstring length at peak tension. At
The hamstring muscles are mostly active during the
present, information is limited regarding what resist-
late swing phase and the start of the stance phase.9
ance training maximally activates the hamstring,
During sprinting the hamstring muscles contract
which exercise is the most specific related to sprint
eccentrically during the late swing and late stance
and targets the hamstrings strength in the most vul-
phases to control knee and hip extension, which
nerable position that occurs in high speed running:
makes the risk of hamstring injury greatest during
the angles seen in late swing phase, that occur with
those phases.10,11 Yu, et al.10 argued that hamstring
a fast eccentric contraction. Training the hamstring
strain injuries may be most likely to occur at the
muscle group is critical for performance and plays
muscle tendon junction during the late stance phase,
an important role in hamstring injury prevention.
and in the muscle belly during the late swing phase.
The Nordic hamstring exercises is commonly used
Different risk factors for these hamstring strains were for prevention of hamstring strains. It is an exercise
identified, which can be categorized in two catego- that is suggested to target the hamstrings effectively
ries: 1) unmodifiable factors like age,12 previous ham- and has been shown to prevent hamstring strains.19
string injury2,12-14 and 2) modifiable factors: hamstring However, whether the angles at which the exercise is
weakness,14,15, muscle fatigue,16 decreased flexibility,17 conducted are similar to the late swing phase has not
poor running technique and altered neuromuscular been studied, and it is not known if hamstring muscle
function.18 Since it is not possible to affect the unmod- activity is high enough during this exercise to elicit a
ifiable factors, only the modifiable factors are of inter- strength training stimulus, which has been suggested
est when aiming to avoid a future hamstring strain. to be at least 70% of a maximal voluntary contrac-
tion.20-22 There may be more effective strength exer-
Hamstring weakness is one of the most common cises like some explosive exercises that may better
risk factors associated with hamstring injuries. It target the hamstring muscles, and at more specific
has been suggested that hamstrings can produce suf- angles and higher movement velocities that resem-
ficient force to counter the force produced by the ble the demands of the late swing and early stance
quadriceps during various movements.15 Thereby, a phase in high speed running.
stronger muscle could provide adequate protection
Therefore, the aim of this study was to examine dif-
from stretching and tearing the muscle fibers.8
ferent hamstring exercises and compare the muscle
Several training studies were conducted to investi- activity in the hamstring muscle group during vari-
gate how to decrease weakness and increase neuro- ous exercises with the muscular activity produced
logical control. Guex, et al.8 presented a conceptual during maximal sprints. The gained information
framework for strengthening the hamstring and for regarding muscle activity during the different ham-
developing specific exercises. They proposed six key string strength exercises could help trainers, physi-
parameters to be considered when developing exer- otherapists, and athletes to develop strength training
cises: contraction type, load, range of motion, angu- programs that could target the hamstrings in an
lar velocity, uni/bilateral exercises, and the kinetic effective way to gain hamstring strength and poten-
chain. Guex, et al.8 concluded that the hamstring tially reduce the chance of hamstring strain during
strength exercises used should be specific to simu- high-speed running.

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 719
MATERIALS AND METHODS of the right leg was shaved, making the electrode
To compare maximal electromyography (EMG) adherence faster. After shaving the leg, the subjects
activity for the hamstring muscle group, during performed a general warm up: 15 min of running
a maximal sprint with several types of hamstring on a non-motorized treadmill. Following the warm-
strength exercises, seven popular hamstring exer- up, the subjects were allowed five minutes of rest,
cises were chosen. The exercises were able to be where they were allowed to drink water. During this
performed without much specific strength equip- rest, electrodes and 21 retroreflective markers were
ment. Some of the chosen exercises, such as the Nor- placed on the hamstring muscles and anatomical
dic hamstring exercise, were selected from existing landmarks.
research papers.27, 28 In addition, some modifications
The test started with a sprint wearing their regular
to the Nordic hamstring exercise that have been sug-
running shoes on the Woodway Curve 2.0 (Wood-
gested to target the hamstrings even more than the
way Inc., Waukesha, USA). This is a non-motor-
standard Nordic hamstring exercise, were included
ized treadmill with curved running surface, which
as well as some less well investigated exercises that
makes it possible for the subjects to run upright and
simulate higher movement velocities seen in high
at their own controlled pace. Furthermore, it made
speed running. All the exercises focused on target-
it possible to easily measure maximal peak veloc-
ing the hamstrings in lengthened conditions, which
ity together with 3D kinematics and muscle activ-
represents the risk situation the hamstrings undergo
ity. After the sprint the subject had five minutes
during the running cycle.
rest before performing one of the seven hamstring
strength exercises. The different exercises were: a)
SUBJECTS the laying hamstring kick, b) the standing hamstring
Twelve male sports students (age 25±6.2 years, kick, c) Nordic hamstring, d) Nordic hamstring with
1.80±7.1 m, body mass 81.1±15.6 kg) participated in return, e) Nordic hamstring + bump, f) Hamstring
the study. Participants were excluded from the study cranes without return, and the g) Hamstring cranes
if they had a former hamstring strain (the previous with return exercise. See Figure 1 for the descrip-
year), or if they had muscular pain or illness that tion and performance of each exercise. Limiting the
could reduce their effort under each exercise. All test to three repetitions per exercise, randomization
participants were familiar with resistance training of the exercise order, and five minutes of recovery1
of the lower extremity. The participants were asked provided between the exercises were employed to
to refrain from any heavy strength training targeting reduce fatigue and an order effect.
the lower body during the 48 hours before testing, in
order to ensure that they were free from strains and Measurements
well trained. Before testing, a written consent was Electromyography (EMG) was used to quantify
contained from the participants. The study was con- muscle activity during the sprint and the various
ducted with approval of the Regional Committee for exercises. Wireless EMG was recorder by using a
Medical Research Ethics and conformed to the latest Musclelab 6000 system and analyzed by MusclelabTM
revision of the Declaration of Helsinki. v10.73 software (Ergotest Technology AS, Lange-
sund, Norway). Before placing the gel coated self-
Procedures adhesive electrodes (Dri-Stick Silver circular sEMG
One to two weeks before testing day each subject Electrodes AE-131, NeuroDyne Medical, USA), the
had a familiarization session with the different exer- skin was shaved, abraded and washed with alcohol.
cises and the sprint on a non-motorized treadmill. The electrodes (11 mm contact diameter and a 2 cm
On testing day, the subjects were briefed about the center-to-center distance) were placed along the
exercise order. The sprint was always conducted first presumed direction of the underlying muscle fiber
(after warm-up) followed by the seven hamstring according to the recommendations by SENIAM 23.
exercises, which were performed in a randomized The electrodes were placed on the right leg on the
order for each subject to avoid the order effect due muscle belly of the biceps femoris, semitendinosus
to fatigue. Before the warm-up, the hamstring area and semimembranosus. The raw EMG signals, sam-

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 720
Figure 1. Performance of the different hamstring exercises. (A) Laying kick: lay on back with on shoulders, hips, and right heel
on the ground, kick left heel up, in order to lift right foot off the ground and land on it again (B) Standing kick: stand on left foot,
lift right knee and then kick out rapidly with the right foot. (C) Nordic hamstrings: with the feet held by a belt, lean forward with
straight hips and back, until unable to hold, release and absorb forces with the hands in an eccentric push up motion. (D) Nordic
with return: Same set up as (C), lean forwards with straight hips until unable to hold any longer, and return to the upright starting
position. (E) Nordic with bump: lean forwards at the limit of your ability to hold, then move the 5kg weight straight forwards and
back as fast as possible. (F) Cranes: flex hips to 90 degrees. Then extend knees until unable to hold it anymore, absorb forces with
the hands in an eccentric push up motion. (G) Cranes with return: flex hips to 90 degrees. Then extend knees until unable to hold
any longer, and return to the starting position.

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 721
pled at 1000 Hz were amplified and filtered using semitendinosus, semimembranosus and biceps) x 8
a preamplifier located as close to the pickup point (exercise) with repeated measures performed. If the
as possible directly connected to the electrodes. The sphericity assumption was violated the Greenhouse-
signals were bandpass filtered (fourth-order Butter- Geisser adjustments of the p-values were reported
worth filter) with cut-off frequencies of 20 Hz and 500 in the results. Post hoc test using Holm-Bonferroni
Hz. The preamplifier had a common mode rejection probabilities adjustment was used to locate signifi-
ratio of 100 dB. The EMG signals were converted to cant differences. The level of significance was set
root mean square (RMS) EMG signals using a hard- at p≤0.05. For statistical analysis purposes SPSS Sta-
ware circuit network (frequency response 20 - 500 tistics v21 (SPSS, Inc., Chicago, IL) was used. All
kHz, integrating moving average filter with 100 ms results are presented as means ± standard devia-
width, total error ± 0.5%). In this study, a compari- tions and effect size was evaluated with (Eta partial
son of muscle activation during the various exercises squared) where 0.01<η2<0.06 constitutes a small
was investigated in relation to the maximal EMG effect, a medium effect when 0.06<η2<0.14 and a
activity that occurred during a maximal sprint. The large effect when η2>0.14 24.
peak RMS converted and filtered data was obtained
from the hamstring exercises and presented as per- RESULTS
centage of the maximum activation. The peak RMS The maximal sprint velocity on the non-motorized
converted and filtered data from the muscles during treadmill was 22.5 ± 2.0 km/t, Significant differ-
the sprint was used as reference (100%). ences in EMG activity for the Semitendinosus (F
= 9.28, p<0.001, η2 = 0.48), Semimembranosus (F
A three-dimensional (3D) motion capture system =14.1 p < 0.001, η2 = 0,56) and Biceps femoris (F
(Qualysis, Gothenburg, Sweden) with eight cameras = 47.45, p < 0.001, η2 = 0.81) were found between
operating with a frequency of 500 Hz was used to the sprint and the seven exercises. Post hoc compari-
track reflective markers, creating a 3D positional son showed that the maximum EMG activity during
measurement. The markers were placed on spinous sprinting was significantly higher compared with all
processes of the fifth lumbar vertebrae, one on each other exercises for the semitendinosus and biceps
side of the body on lateral tip of the acromion, the femoris, while for the semimembranosus the laying
iliac crests, greater trochanters, on the lateral and kick was not significantly different from the sprint
medial condyles of the knee, on the lateral and (Figure 2). In addition, the semimembranosus EMG
medial malleolei, the distal ends of metatarsals I and activity was also significantly higher during the lay-
V. Segments of the feet, lower and upper leg, pel- ing kick compared with the standing kick, and the
vis and trunk were made in Visual 3D v5 software two cranes exercises (Figure 2). For the semitendi-
(C-Motion, Germantown, MD, USA). Joint angles nosus and biceps femoris the maximal EMG activ-
were measured during the sprint and all seven exer- ity during the crane and the crane with return were
cises. 3D motion capture data was synchronized significantly lower than the three different Nordic
with the wireless EMG recordings. The hip and knee hamstrings exercises. For the semimembranosus
joint angles at which maximal muscle activation of only the EMG activity of the cranes were signifi-
the biceps femoris, semitendinosus and semimem- cantly lower than the three Nordic hamstring exer-
branosus occurred were recorded, and were used for cises (Figure 2).
further analysis.
No significant effect between the muscles were
STATISTICAL ANALYSIS found for the hip (F =0.79, p= 0.47, η2=0.14), and
To assess differences in kinematics and EMG activ- knee angle (F =0.46, p= 0.64, η2=0.07) at which the
ity during the sprint and the seven exercises, a maximal EMG activity occurred. However, the hip
One-way ANOVA with repeated measures for each (F =9.1, p<0.001, η2=0.65) and knee joint angles
of three muscles was used. In addition, to compare (F = 13.0, p< 0.001, η2=0.69) at which the maxi-
the kinematics (knee and hip joint angles) at which mal EMG activity occurred was significantly differ-
maximal muscle activity for each muscle occurred ent between the exercises (Figure 3 and Table 1).
in the sprint and the seven exercise a 3 (muscles: Post hoc comparison showed that the knee angle

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 722
standing kick compared with the sprints for all three
muscles (Table 1).

No significant interaction effect was found for the


occurrence at which the maximal EMG activity
occurred for the hip (F =0.23, p= 0.99, η2=0.04) and
knee joint angles (F =.52, p= 0.98, η2=0.08).

DISCUSSION
The aim of this study was to compare hamstring
muscles activity during different hamstring
strengthening exercises with the hamstring muscles
activation during a maximal sprint. The main find-
ings were that maximal EMG activity of the differ-
ent hamstring exercises were on average between
40-65% (Semitendinosus), 18-40% (biceps femoris)
and 40-75% (Semimembranosus) of the max EMG
activity produced by the hamstrings during a sprint.
Cranes showed the lowest muscle activation (in all
muscles) together with the standing kick for the
semimembranosus (Figure 2). In addition, angles at
which the peak EMG activity of the hamstring mus-
cles occurred differed between the two crane exer-
cises (hip angle), standing kick (hip angle) and the
laying kick (knee angle) when compared with the
sprint.

Analyses of hamstring activation reveal that the


maximal sprint resulted in the highest muscle activ-
ity for the semitendinosus and biceps femoris when
compared to all of the hamstring exercises. For the
Figure 2. Maximum EMG activity for semitendinosus, semimembranosus only the laying kick had no sig-
semimembranosus, and biceps femoris during the different nificant difference in level of activation with the
exercises (%) related to the sprint (100%).
sprint, while maximal activation during the other
*Indicates a significant difference between this EMG activity
and to the right of the arrow on a (p<0.05) exercises was lower than activation during the sprint.
†Indicates a significant difference in EMG activity between
It was expected that hamstring activation during the
these two exercises (p<0.05)
sprints would be the highest since it involves rapid
hip and knee joint movements that utilizes the ham-
at which maximal EMG occurred was significantly string to a large degree. Previous authors 25-27 have
lower (more knee flexion) in the laying kick for all found that hamstring muscles are most active during
three muscles compared with the knee joint angle the late swing phase of the sprint, in order to slow
during the sprints. In addition, the knee angle at the forward moving limb. During this part of the run-
which maximal EMG of the biceps occurred during ning gait cycle, the hamstring muscles work mostly
the standing kick was significantly greater (less knee eccentrically, therefore the authors’ chose different
flexion) than the angle during the sprints (Table 1). hamstring strength exercises also suggested to eccen-
The hip angle of maximal EMG was significantly trically stimulate the hamstrings. However, most of
greater (less hip flexion) for the cranes and cranes the studied exercises reported an EMG activity lower
with return and lower (more hip flexion) for the than 70% of maximal EMG activity during sprint.

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 723
Figure 3. The angles (+/-SD) at maximal EMG activity for the hip and knee joints, averaged over all three muscles for each
hamstring exercise and sprint.
*Indicates a significant difference for hip joint angle for this exercise with the sprints (p<0.05)
†Indicates a significant difference for knee joint angle between this exercise and the sprints on a (p<0.05)

Table 1. Hip and knee joint angles at which maximal EMG activity was recorded of the three muscles during
sprint and the seven hamstring strength exercises. All values are reported as degrees, +/- standard deviations

Only the semitendinosus during the three Nordic during sprints. In support of this methodology, Jön-
exercises the and the laying kick demonstrated an hagen, et al. 25 showed that maximal voluntary con-
average activation of respectively around 70% and tractions in the hamstring were equal to maximal
80% and was not significantly different from the muscle activation during maximal sprints. None
sprint. It has been suggested that in order to gain of the investigated exercises in the present study
strength in a muscle, an activity has to be at least reached the level of activation needed for strength
70% of a maximal voluntary contraction. 20,22 In the gain (70%) for the biceps femoris (Figure 2). Muscle
present study, no maximal voluntary contractions activity during the two crane exercises and the stand-
were measured, rather activity during exercises were ing kick were also not of a high enough intensity to
related to the maximal contractions demonstrated be a strengthening stimulus for any of the muscles.

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 724
Despite the relatively low levels of muscle activa- strength to return to starting position. This resulted in
tion (≤70%) during the Nordic hamstrings exercises the same maximal EMG activation during these two
several recent studies have shown that training the crane exercises. It seems that the level of maximal
Nordic hamstring exercise can increase strength and activation determines the degree of forward lean dur-
muscle activation of the hamstrings.28,29 Bourne, et ing these variations of Nordic Hamstring exercise, as
al.28 showed that Nordic hamstring exercise train- the joint angles are similar, and thus these modified
ing promoted the elongation of the fascicles of the Nordic hamstring exercises may be used as variations
biceps femoris long head while it did not promote of the traditional Nordic hamstring during training.
hypertrophy of the biceps femoris long head. They The lower activity in the cranes exercises were
found that it preferentially developed hypertrophy probably caused by the different hip angles (more
of the semitendinosus. This is in accordance with extended) at which maximal hamstring activity was
the present study that shows activation levels in the measured as compared to the sprints (Table 1, Figure
semitendinosus that reached almost 70% compared 3). During the crane exercises subjects first have to
with the biceps femoris of only 40% when compared flex their hips. This increases the load on the ham-
to values during sprinting. Delahunt, et al.29 found string due to the weight of the trunk that is moving
similar average percentage EMG levels for the semi- toward horizontal. From there subjects move for-
tendinosus (65% of eccentric MVC) in the Nordic wards, while the trunk is maintained horizontally.
hamstring exercise, as in the present study. How- This probably causes that the hip angle at which the
ever, after six weeks of Nordic hamstring exercise maximal hamstring EMG activity was measured to
training the subjects increased this percentage to occur at another hip angle, while the knee angles
80% of eccentric MVC. In the present study some were comparable with the Nordic exercises (Table 1,
subjects had much training experience with the cho- Figure 3). The alterations in hip angle in the presence
sen hamstring exercises, while others did not have of the same knee angle likely resulted in a different
as much experience. This may have influenced the effective hamstring muscle length and thereby low-
variation in EMG activity percentage between sub- ered muscle activity that was measured (Figure 2).
jects during these exercises as shown by the large
standard deviations (Figure 2). The standing kick did cause similar, low-level mus-
cle activation as the cranes (Figure 2), which was
It was expected that the hamstring activity would be probably caused by the absence of an added weight
higher in the modifications of the Nordic hamstring that could load the hamstrings. In all other exer-
exercise (Nordic with return and Nordic with bump) cises the trunk causes extra load to the hamstrings,
than the traditional Nordic hamstring exercise, espe- as compared with the sprint, during which the load
cially the Nordic with bump. By leaning forwards on the hamstrings is caused by the lower and upper
as far as possible the subject could hold their body limb movements. The standing kick was included to
with the hamstring muscles and then add the move- investigate whether kicking in a position of higher
ment of pushing and pulling a 5 kg weight (Figure knee extension and flexion than in sprinting would
1), which was projected to increase the activity of the cause an extra load to the hamstrings. The present
hamstring muscles even more. However, this was not data suggests that this was not the case. However,
the case. An explanation for these findings may be adding extra weights around the ankle joint may
that the subjects did not lean as far forward during potentially have increased the hamstrings activity
the modified Nordic hamstrings exercises as shown to a level comparable to the other exercises, but this
in the lower knee angles achieved during the exer- was not investigated.
cise (Table 1 and Figure 3). This causes lower initial
work for hamstrings, but the extra weight that has to The maximal hamstring activity during sprint was
be pushed, or the return movement compensates for measured for all three muscles at around the same
this decrease in leaning. The same was found between time in the late swing phase, which was comparable
the cranes and the cranes with return i.e. less shifting with earlier studies 25-27,30. The angles of Nordic ham-
forward in the cranes with return (lower knee angle) strings and modified hamstring exercises at which
so that the subjects could be sure that they had the maximal hamstring activity occurred was similar to

The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 725
the maximal activity seen during sprints indicating at which peak hamstring activation occurred dur-
that these exercises target the hamstring muscles at ing sprinting, which may in part explain the great
the right part of the running cycle when following potential for hamstring injury prevention shown in
the principle of specificity. The laying kick exercise other studies. Furthermore, the results show that
produced similar EMG activity to the Nordic ham- the explosive laying kick exercise yielded similarly
string exercises, and for the semitendinosus reached high hamstring activation levels but the knee joint
almost the same activity level as in the sprints angle was not specific to the angle at which highest
indicating that this also could be a good hamstring activation occurred during the sprint. This exercise
strength exercise. However, in the present study the must be studied more before the potential as a pro-
knee flexion angle was much more flexed for this phylactic exercise can be determined.
exercise compared to the sprints, which could target
the hamstring muscle at the wrong angle. Yet, sub-
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The International Journal of Sports Physical Therapy | Volume 12, Number 5 | October 2017 | Page 727

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