The document summarizes the facilities and equipment available at the Biotechnology Unit of the University of Southern Mindanao Agricultural Research Center. It describes several existing laboratories for plant tissue culture, isozyme analysis, animal science, virus indexing, and molecular biology. It also proposes additional laboratory facilities that could be added, such as bioreactors, CO2 incubators, environmental chambers, and plant growth chambers. Finally, it lists some common equipment currently available, including a thermal cycler, microcentrifuge, distilling apparatus, and gel electrophoresis apparatus.
The document summarizes the facilities and equipment available at the Biotechnology Unit of the University of Southern Mindanao Agricultural Research Center. It describes several existing laboratories for plant tissue culture, isozyme analysis, animal science, virus indexing, and molecular biology. It also proposes additional laboratory facilities that could be added, such as bioreactors, CO2 incubators, environmental chambers, and plant growth chambers. Finally, it lists some common equipment currently available, including a thermal cycler, microcentrifuge, distilling apparatus, and gel electrophoresis apparatus.
The document summarizes the facilities and equipment available at the Biotechnology Unit of the University of Southern Mindanao Agricultural Research Center. It describes several existing laboratories for plant tissue culture, isozyme analysis, animal science, virus indexing, and molecular biology. It also proposes additional laboratory facilities that could be added, such as bioreactors, CO2 incubators, environmental chambers, and plant growth chambers. Finally, it lists some common equipment currently available, including a thermal cycler, microcentrifuge, distilling apparatus, and gel electrophoresis apparatus.
Familiarization of Laboratory Facilities and Equipment in a Biotech Laboratory
1. Laboratories housed at the Biotechnology Unit of Southern Mindanao Agricultural Research
Center (USMARC).
Laboratory Uses and Importance
1. Plant tissue culture -used to produce clones of a plant in a method known as laboratory micro propagation. -To clean particular plants of viral and other infections and to quickly multiply these plants as 'cleaned stock' for horticulture and agriculture. 2. Isozyme laboratory - performs electrophoretic analyses of genetically conditioned enzyme variation by separating multiple forms of enzymes in a solid matrix - detection of genetic variation within and among populations, the identification of plant clones, hybrids and their distribution in an area, and studies of evolutionary dynamics in natural plant populations as well as plant mating systems and genetic diversity in rare or invasive species. 3. Animal science laboratory - housing, caretaking, genetic and microbial quality control, anesthesia and analgesia, feeding, experimental techniques, etc - contribute to the reliability and reproducibility of the results of animal experiments and to the life quality of laboratory animals that are used for research. 4. Virus-indexing laboratory -Production of virus antibody by molecular cloning and expression technology -Identification and characterization of plant viruses occurring in economically important crops in, preparation and preservation of virus antibodies, and development of indexing techniques. 5. Molecular and biotechnology - it is already used widely in manufacturing insulin for laboratory diabetics, and is central to a range of other biomedical products. -provides you with the right mixture of sound scientific knowledge and commercial expertise to succeed in the emerging world of biotechnology 2. Laboratory facilities that might be added to the laboratory at Biotech unit University of Southern Mindanao Agricultural Research Center. (USMARC)
Laboratory Facilities Uses
1. Bioreactor -used for precise phototrophic cultivation of algae and cyanobacteria. They feature a unique combination of the cultivator and monitoring device. Light power and spectral composition as well as the temperature and aeration gas composition can be set with a high accuracy. The illumination unit can be removed easily prior to sterilization and then clicked back into position following the procedure. 2. CO2 Incubator -plate & sample drying problems are greatly reduced with high humidity levels using this device. An anticipative logic system controlling both temperature and CO2 levels. 3. Environmental -with programmable controller provides precise Chamber temperature and humidity control. 4. FTIR The application of programmable gain amplifier, high Spectrometer accuracy A/D converter and embedded computer improves the accuracy and reliability of the whole system. It is user friendly, rich function software enables easy, convenient and flexible operation. Spectrum collect, spectrum conversion, spectrum collect, spectrum conversion, spectrum processing, spectrum analyzing, and spectrum output function etc. can be performed. 5. Particle Counter -traditional design with measuring unit, computer and oscillograph. Advance pulse measurement technology, more than 8000 channels. It is replaced with a pressure sensor, provides more accurate measurement and control of degree of vacuum, and higher automation. 6. Particle Size -it is used to measure fog drops: Its structure can be Analyzer changed according to the requirements. A Fourrier Lens with different focal length can be used to change the measuring range. 7. Plant Growth -are ideal for agriculture or biotechnology testing growth of Chamber plants in an artificial climatic condition. It controls temperature, relative humidity and illumination. The wide variety of temperatures and light intensity patterns that are essential in plant research can now be accurately reproduced and controlled with the updated range by Plant Growth Chambers. 8. X-Ray -it can simultaneously analyze elements ranging from Na to Spectrometer 92U. It is a very rapid method with determination time from 10 seconds to 1000 seconds. 9. Safety Cabinets -are designed for balance applications in the safety storage industry. Handling of harmful and toxic compounds are done safely thus reducing the risk of explosion. Safety Cabinets are high performance unit that are easy to use, clean and maintain. Particular attention is also paid to reliability and smooth running. 10. Water -it is a compact system that purifies water for general Purification System laboratory use or as a feed source for ultrapure system. Utilizing tap water for feed. 3. List of available equipment in Biotech unit University of Southern Mindanao Agricultural Research Center (USMARC).
Laboratory Facilities Uses
1 Thermal cycler - is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR) 2 Microcentrifuge - is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity. 3 Distilling Apparatus -a set of apparatuses that uses distillation, a common operation in many laboratories for the purpose of separating and/or purifying components of a liquid mixture the apparatus used consists of three major parts: distillation flask (or ‘pot’) to heat the mixture and volatilize the components, a condenser to cool the vapors back to liquid state, and a collection vessel. 4 Fumehood - is a type of local ventilation device that is designed to limit exposure to hazardous or toxic fumes,vapors or dusts. 5 Pipetor/micropipettor -is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser 6 Vortex - is a simple device used commonly in laboratories to mix small vials of liquid. 7 Water bath -is a device or a vessel for regulating the temperature of anything subjected to heat, by surrounding the vessel containing it with another vessel containing water which can be kept at a desired temperature. 8 Horizontal gel -also called Agarose gel electrophoresis because it uses electrophoresis Agarose gel. It maintains a uniform electric field across apparatus the gel, provide cooling to prevent thermal artifacts, and allow access to the gel for sample loading and monitoring the run. 9 Gel documentation - is equipment widely used in molecular biology system laboratories for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels. These gels are typically stained with ethidium bromide or other fluorophores such as SYBR Green 10 Polyacrylamide gel - a gel electrophoresis device that uses acrylamide gel that electrophoresis is use in a separation method frequently used to analyze DNA fragments generated by restriction enzymes, and it is a convenient analytical method for determining the size of DNA molecules in the range of 500 to 30,000 base pairs. It can also be used to separate other charged biomolecules such as dyes, RNA and proteins. 11 Microwave oven -is a device that heats food by bombarding it with electromagnetic radiation in the spectrum causing polarized molecules in the food to rotate and build up thermal energy in a process known as dielectric heating. 12 Stirrer/hot plate -is a portable self-contained tabletop small appliance that features one, two or more gas burners or electric heating elements. A hot plate can be used as a stand-alone appliance, but is often used as a substitute for one of the burners from an oven range or the cook top of a stove. 13 Electronic digital -is a weighing scale that is used for measuring substances. balance/Analytical It can be found in place where large quantities of food are balance prepared like hotel kitchens and institutional kitchens. People also use them at home. They are easier to use and they provide more accurate readings. 14 Biofreezer -is a kind of freezer that user a topical over-the- counter gel for pain relief consisting of menthol with and other ingredients. 15 Ice maker - may refer to either a consumer device for making ice, found inside a home freezer; a stand-alone appliance for making ice, or an industrial machine for making ice on a large scale. 16 Autoclave/sterilizer - is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the load and the contents. 17 Binder -It is suitable for all sensitive incubation applications and ensures optimal cell growth. 18 Dual action shaker -is a device that have Orbital or reciprocating shaking action over a wide speed range (10 to 300 rpm) offering an ideal solution for your mixing needs 19 Beakers -is a simple container for stirring, mixing and heating liquids commonly used in many laboratories. 20 Cylinders/Graduated -is a piece of laboratory equipment used to measure the cylinders volume of a liquid. Graduated cylinders are generally more accurate and precise than laboratory flasks and beakers. 21 Erlenmeyer flask -The tapered sides and narrow neck of this flask allow the contents of the flask to be mixed by swirling, without risk of spillage, making them suitable for titrations. Such features similarly make the flask suitable for boiling liquids. Hot vapors condense on the upper section of the Erlenmeyer flask, reducing solvent loss. Erlenmeyer flasks' narrow necks can also support filter funnels. 22 Syringe -is a simple pump consisting of a plunger that fits tightly in a tube. The plunger can be pulled and pushed along inside a cylindrical tube (called a barrel), allowing the syringe to take in and expel a liquid or gas through an orifice at the open end of the tube. 23 Thermometers -is a device that measures temperature or temperature gradient using a variety of different principles.