Book of Abstracts 2019

THE INTERNATIONAL SYMPOSIUM ON ANIMAL SCIENCE
ISAS 2019
Book of Abstracts
June, 03rd – 08th, 2019. Herceg Novi, Montenegro

Organizers
UN IV E RS I T Y O F NO V I SA D UNIVERSITY OF BELGRADE
FAC U L TY O F FAC U L TY O F
A GR IC U L TU R E A GR IC U L TU R E
DE PA R TM EN T O F A N IM AL INSTITUTE O F AN IM A L
SC I EN C E SC I EN C E
21000 Novi Sad, Trg D. Obradovića 8

Tel.:++(021) 6350-711; 4853-308; 11080 Zemun-Belgrade, Nemanjina 6
Fax:++(021) 6350-019 Tel.:++(011) 2615-315; 2197-425;
web:http://www.polj.uns.ac.rs Fax:++(011) 3161-490
e-mail: stocarstvo@polj.uns.ac.rs web: www.agrif.bg.ac.rs
e-mail: insstoc@agrif.bg.ac.rs
THE INTERNATIONAL SYMPOSIUM ON

ANIMAL SCIENCE (ISAS) 2019
03-08.06.2019. Herceg Novi, Montenegro
BOOK OF ABSTRACTS
Co- Organizers
University of Montenegro, Biotechical Faculty - Montenegro
Slovak University of Agriculture in Nitra

Faculty of Biotechnology and Food Sciences – Slovakia
National Agricultural and Food Centre

Research Institute for Animal Production in Nitra – Slovakia
Banat University of Agricultural Sciences and Veterinary Medicine,

Bioengineering faculty of animal resources - Timisoara, Romania
Co-Sponsorship
European Society of Agricultural Engineers

ISBN: 978-86-7520-467-1
THE INTERNATIONAL SYMPOSIUM ON ANIMAL SCIENCE (ISAS) 2019

Book of Abstracts
Publisher
University of Novi Sad, Faculty of Agriculture
21000 Novi Sad, Trg D. Obradovića 8
Tel.:++(021) 6350-711; 4853-308;
polj.uns.ac.rs
On behalf of Publisher
Prof. dr Nedeljko Tica
Editor in Chief
Prof. dr Lidija Perić
Paper review
All papers reviewed by The International Board of Reviewers
Recorded by
Feljton, Stražilovska 17, Novi Sad
Cover
Elsa Chang,
www.elsasketch.com
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CIP - Каталогизација у публикацији

Библиотеке Матице српске, Нови Сад
636(048.3)
INTERNATIONAL Symposium on Animal Science (2019 ; Herceg Novi)

Book of abstracts [Elektronski izvor] / The International Symposium on Animal
Science (ISAS) 2019, 3-8. 6. 2019, Herceg Novi, Montenegro ; [editor in chief Lidija
Perić]. - Novi Sad : Faculty of Agriculture, 2019. - 1 elektronski optički disk (CD-ROM)
: tekst ; 12 cm
Nasl. sa naslovnog ekrana. - Napomene i bibliografske reference uz tekst.
ISBN 978-86-7520-467-1
а) Сточарство -- Апстракти
COBISS.SR-ID 329515527
2
ISAS 2019
Scientific Committee
Prof. dr Lidija Perić, Faculty of Agriculture Novi Sad, Serbia, chairman

Prof. dr Zoran Popović, Faculty of Agriculture Beograd, Serbia
Prof. dr Dragan Radojković, Faculty of Agriculture Beograd, Serbia
Prof.dr Predrag Perišić,Faculty of Agriculture Beograd, Serbia
Prof. dr Adriana Kolesárová, Faculty of Biotechnology and Food Science, Nitra,
Slovakia
Prof. dr Peter Chrenek, Research Institute for Animal Production Nitra, Slovakia
Prof. dr Lavinia Stef, Faculty of Animal Science and Biotechnology, Timisioara,
Romania
Prof.dr Istvan Komlosi, Faculty of Agricultural and Food Sciences and Environmental
Management, University of Debrecen, Hungary
Prof.dr Karoly Dublecz, Department of Animal Science and Animal Husbandry,
University of Pannonia, Veszprem, Hungary
Prof. dr Pero Mijić, Faculty of Agriculture Osijek, Croatia
Prof. dr Mirjana Baban, Faculty of Agriculture Osijek, Croatia
Prof. dr Ante Ivanković, Faculty of Agriculture Zagreb, Croatia
Prof. dr Muhamed Brka, Faculty of Agricultural and Food Sciences Sarajevo, BIH
Prof.dr Refik Šahinović, Biotechnical faculty, Bihać, BiH
Prof. dr Janez Salobir, Biotechnical faculty Ljubljana, Slovenia
Prof. dr Peter Dovč,Biotechnical faculty Ljubljana, Slovenia
Prof. dr Đoko Bunevski,Faculty of Agricultural Sciences and Food Skopje, North
Macedonia
Prof.dr Božidarka Marković, Biotechnical faculty Podgorica, Montenegro
Prof.dr Stoja Jotanović,Faculty of Agriculture Banja Luka, Bosnia and Herzegovina
Prof.dr Mirjana Đukić Stojčić, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Ivan Radović, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Igor Jajić, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Ivan Pihler, Faculty of Agriculture Novi Sad, Serbia
Doc dr Dejan Beuković, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Denis Kučević, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Saša Dragin, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Miloš Beuković, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Dragan Glamočić, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Aleksandar Božić, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Marko Cincović, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Vesna Rodić, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Rodne Nastova, Institute of Animal Science, Skopje, North Macedonia
Prof.dr Gordana Rokvić, Faculty of Agriculture Banja Luka, Bosnia and Herzegovina
3
Organizing Committee
Prof.dr Nedeljko Tica, Faculty of Agriculture Novi Sad, Serbia

Prof. dr Snežana Trivunović, Faculty of Agriculture Novi Sad, Serbia - president
Dr Vuk Radojević, Faculty of Agriculture Novi Sad, Serbia
Prof. dr Branislava Belić, Faculty of Agriculture Novi Sad, Serbia
Prof. dr Miomir Jovanović, Biotechnical faculty Podgorica, Montenegro
Prof.dr Milan Marković, Biotechnical faculty Podgorica, Montenegro
Prof. dr Nicolae Pacala, Faculty of Animal Science and Biotechnology, Timisioara,
Romania
Prof.dr Ioan Pet, Faculty of Animal Science and Biotechnology, Timisioara, Romania
Prof. dr Jan Tomas, Faculty of biotechnology and Food Science, Nitra, Slovakia
Prof. dr Dana Peškovičova, National Agricultural and Food Centre, Nitra, Slovakia
Doc. dr Dragan Stanojević, Faculty of Agriculture Belgrade, Serbia
Doc. dr Radomir Savić , Faculty of Agriculture Belgrade, Serbia
Doc. dr Vesna Davidović, Faculty of Agriculture Belgrade, Serbia
Prof.dr Branko Ćupina, Faculty of Agriculture Novi Sad, Serbia
Prof. dr Dragan Žikić, Faculty of Agriculture Novi Sad, Serbia
Prof.dr Miroslav Plavšić, Faculty of Agriculture Novi Sad, Serbia
Dr Siniša Bjedov, Faculty of Agriculture Novi Sad, Serbia
Mr Srđan Ljubičić, Faculty of Agriculture Novi Sad, Serbia
Secretariat
Mr Željka Jurakić, Faculty of Agriculture Novi Sad, Serbia

MSc Ljiljana Macura, Faculty of Agriculture Novi Sad, Serbia
Helena Šifler Zekić, Faculty of Agriculture Novi Sad, Serbia
4
Symposium is supported by:
Provincial Secretariat for Science and Technological Development
Symposium is sponsored by:
General sponsor
Delta Agrar Beograd
Golden sponsor
Nidalav DOO Novi Sad
Partner support
Edufarm Crvenka
Konzul D.O.O. Novi Sad
Agroprodukt Šinković Bečej
Patent Co. - Mišićevo
5
CONTENT
1. Djedović R., Stanojević D., Bogdanović V., Petrović M., Trivunović S., Štrbac Lj.,
Janković D., Rogić B.: GENETIC TREND OF MILK YIELD IN STANDARD
LACTATION AND LIFETIME MILK PRODUCTION IN BLACK AND
WHITE COWS ..................................................................................................... 9
2. Korabi N., Mijić, P., Baban, M.: DEVELOPMENT TREND IN THE HORSE
BREEDING SECTOR OF THE REPUBLIC OF CROATIA .......................... 10
3. Mirkov M., Radović I., Vasiljević V., Konjević S.: EXAMINATION OF THE
EFFECT OF THE THICKNESS BACON IN GILTS PERFORMANCE TEST
ON THE SIZE OF THE LITTER ...................................................................... 11
4. Plavšić M., Kučević D., Čobanović K., Božić A., Stankovski S.:
RELATIONSHIP BETWEEN MILK PRODUCTION AND WHITE CHEESE
QUALITY OF HOLSTEIN COWS DURING HEAT STRESS ...................... 12
5. Stanojević D., Djedović R., Bogdanović V., Stepić S., Raguž N., Lazarević M.,
Kučević D., Štrbac Lj., Janković D.: EFFECT OF GENES SHARE HOLSTEIN
FRISIAN BREED ON LONGEVITY TRAITS DAIRY COWS ..................... 13
6. Trivunović S., Štrbac Lj., Šaran M. , Obad M., Janković D., Bunevski Gj.,
Radinović M.: ESTIMATION OF GENETIC PARAMETERS FOR MILK
TRAITS IN DIFFERENT LACTATIONS OF HOLSTEIN-FRIESIAN
CATTLE USING RANDOM REGRESSION MODEL .................................. 14
7. Vašíček J., Baláži A., Tomková M., Miššík J., Tomka M., Chrenek P.: EFFECT
OF THE CRYOPRESERVATION ON THE ELEMENTAL COMPOSITION
OF RABBIT ENDOTHELIAL PROGENITOR CELLS ................................. 15
8. Vašíček J., Baláži A., Bauer M., Chrenek P.: CHANGES IN THE RELATIVE
EXPRESSION OF SELECTED MARKERS DURING THE CULTURE OF
RABBIT ENDOTHELIAL PROGENITOR CELLS ........................................16
9. Pihler I., Zarubica B., Kučević D., Dragin S., Žarković I., Mekić C., Lazić M.: THE
INFLUENCE OF MOTHER'S AGE ON BIRTH WEIGHT OF LAMBS ..... 17
10. Baldovská S., Michalcová K., Kolesárová A.: THE IMPACT OF
POMEGRANATE ON PRESENCE OF STEROID HORMONE
RECEPTORS IN OVARIAN CELLS .................................................................18
11. Becskei ZS.,
Savić M., Ilić T., Sotiraki S., Charlier J.: EXTENSITY OF
HELMINTOSES OF SHEEP IN BAČKA REGION OF SERBIA .................19
12. Wafaa A. Abd El-Ghany, Hanan A. Ahmed and Ali Z. Qandoos:
ANTIMICROBIAL SENSITIVITY PATTERN OF EGYPTIAN AVIAN
PASTEURELLA MULTOCIDA STRAINS ......................................................... 20
13. Kolesárová A., Baldovská S., Michalcová K., Gálik B.: FRUIT BYPRODUCTS:
A POSSIBLE MODULATORS OF OVARIAN STEROIDOGENESIS IN
VITRO .....................................................................................................................21
6
14. Rodić V., Perić L., Đukić Stojčić M., Rezar V., Relić R., Despotović J.:
STAKEHOLDERS’ PERCEPTIONS OF KEEL BONE DAMAGE IN
LAYING HENS: EVIDENCE FROM SERBIA ...............................................22
15. Božić A., Gaško J., Beuković M., Beuković D. Plavšić M.: THE EFFECT OF
NUTRITION IN THE FINISHING STAGE ON BISON MEAT YIELD AND
QUALITY SCORE ...............................................................................................23
16. Levart A. Rezar V., Leskovec J., Pirman T., Butinar B., Bučar Miklavčič M.,
Podgornik M., Salobir J.: UTILIZATION OF OLIVE BY-PRODUCTS IN
NON-RUMINANT NUTRITION .......................................................................24
17. Malenčić Đ., Miladinović J., Kiprovski B., Mikulić M., Hogervorst J., Prvulović
D.: COLORED SOYBEAN SEEDS: A NEW SOURCE OF FUNCTIONAL
FEED IN ANIMAL NUTRITION .......................................................................25
18. Perić L., Dublecz K., Žikić D., Đukić Stojčić M., Pál L.: EFFECT OF LOW
PROTEIN DIETS ON OCCURRENCE OF FOOTPAD DERMATITIS IN
BROILERS ...........................................................................................................26
19. Pirman T., Levart A., Rezar V., Vrecl Fazarinc M., Salobir J.: INTESTINAL
MORPHOLOGY AND VOLATIL FATTY ACIDS CONTENT IN BROILER
CHICKEN IN DEPENDANCE OF ADDITION OF OLIVE LEAF OR
MARIGOLD EXTRACTS IN N-3 PUFA ENRICHED DIET ......................... 27
20. Radonjić D., Marković B., Marković M., Stešević D., Đokić M., Jokanović O.:
EFFECT OF PHENOLOGICAL PHASE ON FLORISTIC COMPOSITION
AND BIOMASS QUALITY OF LOWLAND PASTURE .................................28
21. Rezar V., Levart A, Pirman T. Nemec Svete A., Kompan R., Salobir J.:
THE INFLUENCE OF OLIVE LEAVES (OLEA EUROPEA) OR
MARIGOLD (CALENDULA OFFICINALIS) EXTRACTS ON OXIDATIVE
STATUS OF BROILERS FED N-3 PUFA ENRICHED DIETS ...................... 29
22. Bujanić, M., Martinković, F., Štimac, I., Škvorc, N., Konjević, D.:
GASTROINTESTINAL PARASITES OF WILD BADGER
(MELES MELES) AT MEDVEDNICA NATURE PARK – PRELIMINARY
RESULTS ..............................................................................................................30
23. Šoštarić-Zuckermann, I.-C., Škvorc, N., Bujanić, M., Konjević, D.:
FASCIOLOIDOSIS AND FASCIOLOSIS IN WILD BOAR (SUS SCROFA
L.) ON HISTOLOGICAL LEVEL ......................................................................31
24. Nedić N., Vojt D.: UNTREATED BEE COLONIES AND THEIR
DEVELOPMENT IN THE COURSE OF A YEAR .......................................... 32
25. Đukić Stojčić M., Perić L.: THE EFFECT OF HEN AGE OF ON THE
QUALITY CHARACTERISTICS OF TABLE EGGS ....................................33
26. Jokanović O., Marković B., Mirecki S.: SENSORY CHARACTERISTICS OF
KUČI CHEESE ....................................................................................................34
27. Marković G., Mladenović J., Pantović J.: BASIC BIOLOGICAL VALUE OF
SOME TYPES OF CANNED FISH ...................................................................35
28. Mirković M., Mirković N., Ilić V., Puđa P., Miočinović J., Paunović D., Radulović
A.: HYGIENIC QUALITY OF WHITE CHEESE PRODUCTION IN
KRALJEVO REGION .........................................................................................36
7
29. Radulović, Z., Mirković, M., Mirković, N., Paunović, D.: HYGIENIC AND
HEALTH SAFETY OF FRESH CHICKEN MEAT ........................................37
30. Bošnjak D., Rodić V., Vukelić N.: BASIC INDICATORS OF LIVESTOCK
PRODUCTION IN SERBIA ...............................................................................38
31. Chrenekova M., Kubovicova E.: ANIMAL PRODUCTION EMPLOYMENT IN
THE SLOVAK REPUBLIC .................................................................................39
32. Sabev S.: ENDOSCOPIC STUDY OF UPPER RESPIRATORY TRACT
DISORDERS IN HORSES .................................................................................. 40
8
INTERNATIONAL SYMPOSIUM ON ANIMAL SCIENCE (ISAS) 2019
3rd to 8th June 2019. Herceg Novi, Montenegro
_______________________________________________________________________________
GENETIC TREND OF MILK YIELD IN STANDARD LACTATION AND

LIFETIME MILK PRODUCTION IN BLACK AND WHITE COWS
Djedović R.1, Stanojević D. 1, Bogdanović V.1, Petrović M. 2, Trivunović S. 3, Štrbac Lj.3,
Janković D.3, Rogić B. 4
Abstract: Research objective was to determine genetic trend of milk yield in standard
lactation of 305 days and lifetime milk production as one of very important indicators of
cow’s longevity and cost benefit. Research included 16539 animals raised on 7 farms of
the Agricultural Corporation Belgrade in the period from 1987 to 2013. The animals
belong to the group of Black and White cattle and are in the phase of crossbreeding with
Holstein Friesian breed. An average share of Holstein Friesian breed genes in trial period
was 78%. Genetic trend was calculated on the basis of breeding values of 272 bull-sires
used for artificial insemination of cows in previously mentioned population. Breeding
values were calculated by REML method by means of an animal model. A method of
linear regression was used for establishing genetic trend with bull breeding value as a
dependent variable and the year of birth as an independent variable. A slightly positive
genetic trend for milk yield in standard lactation (bxy =5.12 kg) was determined i.e. a
genetic improvement of 5.12 kg milk was achieved at an annual level. The value of
coefficients of linear regression for lifetime milk production was bxy =-7.98 and indicates
existence of a negative genetic trend, i.e. lifetime milk production averagely decreased by
7.98 annually. Determined values of regression coefficients for both studied traits were
highly statistically significant (P<0.01).
Keywords: genetic trend, milk yield in standard lactation, lifetime milk production, cows
1
Djedović Radica, PhD, full professor; Stanojević Dragan, PhD, assistant professor; Bogdanović
Vladan, PhD, full professor, University of Belgrade, Faculty of Agriculture, Zemun, Serbia;
2
Petrović Milun, PhD, associated professor, University of Kragujevac, Faculty of Agriculture,
Čačak, Serbia;
3
Trivunović Snežana, PhD, full professor; Štrbac Ljuba, PhD, assistant; Janković Dobrila, PhD,
University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia;
4
Rogić Biljana, PhD, assistant professor, University of Banja Luka, Faculty of Agriculture Banja
Luka, Bosnia and Herzegovina;
Corresponding author: Djedović Radica, email: genrad@agrif.bg.ac.rs
9
_______________________________________________________________________________
DEVELOPMENT TREND IN THE HORSE BREEDING SECTOR OF

THE REPUBLIC OF CROATIA
Korabi N., 1, Mijić, P. 2, Baban, M.2
Abstract: Analysis of the statistical data on equidae breeding and trends in the horse
breeding sector of the Republic of Croatia (Ministry of Agriculture - GI, 2018) determined
that the number of registered equidae has been grown at an annual level from 1.5% to 5.5%
due to the establishment of the Central Register of equidae, systematic animal marking and
registration into the Uniform Register of Domestic Animals. The Republic of Croatia has
27,360 equidae, being an increase of 3,3% compared to 2017. Increase of, 1.9% refers to
23,649 horses of the aforesaid number. The above mentioned number of equidae is owned
by 6608 owners, being also an increase of 3.2%. The most populated breed group consists
of cold-bloods (58%), while the last decade has been known for a slight increase in the
warm-bloods number. The intensive popularization of horse breeding in the Republic of
Croatia, the implementation of breeding programs of breeders' associations, breeding
associations as well as the competent body for different breeds, the realization of the
National Program of the Subsidy of the Breeding Programs implementation for warm-
blooded breeds and Breeding horse types led to the trend of horse breeding in the period
2015 – 2020. The regulations on the implementation of direct subsidy to agriculture and
the IAKS rural development measures in 2019 have significantly increased the former
amounts of EUR 200 per conditional head of the original and protected breeds (EUR
396.32 for the Croatian cold-blood, EUR 404.98 for the Croatian Posavina horse, EUR 502
,04 for Medjimurje horse, 387,11 EUR for Lipizzans). Furthermore, the horse breeding
sector is included in the "blue diesel" quota allocation system. The costs arising from the
Order on animal protection measures from contagious and parasitic diseases are also co-
financed. Not only does horse breeding show a positive trend in breeding sense but also in
all segments of horses use by numerous organized activities with horses, equestrian sports
competitions, recreational tourist activities including cultural folk events.
Keywords: equidae, horse breeding, development trend, coldbreeds, warmbreeds
1
Korabi Nidal, PhD, Ministry of Agriculture, Department for Equidae Breeding and Animal Gene
Bank, Zagreb, Croatia;
2
Mijić Pero, PhD, full professor; Baban Mirjana, PhD, full professor, University of Josip Juraj
Strossmayer, Faculty of Agrobiotechnical Sciences, Osijek, Croatia.
Corresponding author: Baban Mirjana, email: mirjana.baban@fazos.hr
10
_______________________________________________________________________________
EXAMINATION OF THE EFFECT OF THE THICKNESS BACON IN THE

GILTS PERFORMANCE TEST ON THE SIZE OF THE LITTER
Mirkov M. 1, Radović I.1, Vasiljević V.1, Konjević S.1
Abstract: Defining, collecting and analyzing data from the farm enables to better
understand the technological processes on the farm and to see the selection effect, which
aims at improving the technological processes on the farm, and that additional information
makes it easier to identify animals that are worse than average population in production.
One of the parameters taken into account when selecting the animals for breeding through
breeding values of animals is the thickness of the back and side bacon. The thickness of the
back and side bacon used to examine the impact on the size of the litter is obtained by
ultrasound measurement of 954 gilts at the end of the performance test for genotype
Yorkshire. After measuring the values of the thickness of the back and side bacon, were
collected and the average value is taken, which in this paper represents the thickness of the
bacon. The average thickness of bacon in gilts was 14.15 ± 3.24 mm, while the coefficient
of variation was 22.92%.
The size of the litter by sows is a parameter used to compare the productivity of the
breeding animals between themselves and represents an economically important trait that
is closely related later with the number of weaning piglets, which is essential for achieving
viable production. The average number of live-born piglets in gilts that completed the
performance test and which later farrowed is 9.87 ± 2.73, while the coefficient of variation
is 27.68%.An increase in reproductive performance, with no harmful effects on the
survival of piglets in gilts, has recently been the target for pig production. Therefore, this
paper has the task of examining whether there is an effect of bacon thickness on the size of
litter in the first-class. The obtained results indicate that there is no statistically significant
effect on the litter size (> 0.05). For a more detailed examination of the effect of bacon
thickness on the size of the litter, as well as for checking whether certain bacon
thicknesses, regardless of other bacon thickness values, affect the size of the litter, a
Duncan test was made. The obtained results indicate that there is no statistically significant
effect on the litter size (> 0.05).
Keywords: litter size, thickness of bacon, sows, performance test
1
Mirkov Mile, MSc, assistant; Radović Ivan, PhD,professor; Vasiljević Velibor, associate;
Konjević Slobodan, associate University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia;
Corresponding author: Mirkov Mile, email: mile.mirkov@stocarstvo.edu.rs
11
_______________________________________________________________________________
RELATIONSHIP BETWEEN MILK PRODUCTION AND WHITE CHEESE

QUALITY OF HOLSTEIN COWS DURING HEAT STRESS
Plavšić M.1, Kučević D. 1, Čobanović K.1, Božić A.1, Stankovski S.2
Abstract: Aim of this research was to investigate ambient conditions (temperature and
humidity – THI index) in barn of milking 164 Holstein-Friesian cows and its utilization on
milk production and quality of cheese made from that milk. Cows were kept in free – stall
system. Experiment starts at July, 2018 and ended at December, 2018. Milk yield was
measured daily, milk and cheese quality monthly. Milk samples were analyzed using
Combifoss TMFT+ (fat and protein content and somatic cells count) in Laboratory for milk
quality control at the Faculty of Agriculture, Animal Science Department, Novi Sad. White
cheese samples were analyzed in terms of chemical composition and textural properties by
reference methods at the Faculty of Agriculture, Belgrade. THI (temperature-humidity
index) ranged from 41.11 till 78.35 during six months measuring (average 60.49). Milk
yield was lower (16.31 and 19.97 kg) in July and August when the THI values were
highest 71.41 and 70.56 compared with other months of research. Differences in the milk
yield between individual months were, in most cases were significant. Values of THI in
July and August were not significant, but compared them with other months, differences
were exists (p <0.001). Milk fat content was lower in first two months of experiment (3.54
and 3.51) compared with other months of investigation (3.95; 4.19; 4.12 and 4.18
respectively). Milk protein content was lowest in July (3.22%) when THI was highest
(76.79). THI did not significantly affect the chemical composition of white cheese. Content
of milk fat in cheese was lowest in July (16.05 %) and was statistically significant (p
<0.01) compared with other months in research period. Differences in August, September,
October, November and December of milk fat content in cheese were not statistically
significant.
Keywords: cows, heat stress, milk yield, white cheese
1
Plavšić Miroslav, PhD, full professor; Kučević Denis, PhD, associate professor; Čobanović
Ksenija, PhD, assistant professor, Božić Aleksandar, PhD, full professor, University of Novi Sad,
Faculty of Agriculture, Animal Science Department, Novi Sad, Serbia;
2
Stankovski Stevan, full professor, University of Novi Sad, Faculty of Technical Sciences, Novi
Sad, Serbia.
Corresponding author: Plavšić Miroslav; email: miroslav.plavsic@stocarstvo.edu.rs
12
_______________________________________________________________________________
EFFECT OF GENES SHARE HOLSTEIN FRISIAN BREED ON

LONGEVITY TRAITS DAIRY COWS
Stanojević D. 1, Djedović R.1, Bogdanović V.1, Stepić S.1, Raguž N. 2,
Lazarević M. 3, Kučević D.4, Štrbac Lj. 4, Janković D.4
Abstract: The research was conducted with an aim of assessing the effect of share of
Holstein Frisian genes on longevity of Black and White cows. The research included
16539 Black and White breed cows raised in the period from 1981 to 2013 on 7 farms of
the Agricultural Corporation of Belgrade. Data regarding longevity, productivity and origin
of these animals were known. The animals belong to the group of Black and White cattle
and are in the phase of crossbreeding with Holstein Friesian breed. An average share of
Holstein Friesian breed genes in trial period was 78%. On the basis of share of Holstein
Frisian genes all cows are divided into 5 groups ((I group- animals with 0 to 50% share of
genes of Holstein Friesian breed; II group- animals with 51 to 75% share of genes of
Holstein Friesian breed; III group- animals with 76 to 87.5% share of genes of Holstein
Friesian breed; IV group- animals with 88 to 93.75 share of genes of Holstein Friesian
breed; V group- animals with more than 93.5% share of genes of Holstein Friesian breed).
The analysis includes following longevity traits: length of productive life (LPL), lifetime
milk yield (LMY) and number of lactation (NL). The analysis determined statistically
significantly impact for all analyzed longevity traits. A decrease in the duration of length
of productive life, lifetime milk yield and the number of lactations have been identified
with an increase in the share of Holstein Friesian genes in dairy cows.
Keywords: longevity, Holstein Frisian breed, dairy cows
1
Stanojević Dragan, PhD, assistant professor; Djedović Radica, PhD, full professor; Bogdanović
Vladan, PhD, full professor; Stepić Stefan, MSc, assistant, University of Belgrade, Faculty of
Agriculture, Zemun, Serbia;
2
Raguž Nikola, PhD, associated professor, University Josip Juraj Strossmayer Osijek, Faculty of
Agriculture, Osijek, Croatia;
3
Lazarević Marina, BSc, research assistant, Institute for Animal Husbandry, Belgrade, Serbia;
4
Kučević Denis, PhD, associated professor; Štrbac Ljuba, PhD, assistant; Janković Dobrila, PhD,
University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia.
Corresponding author: Stanojević Dragan, email: stanojevic@agrif.bg.ac.rs
13
_______________________________________________________________________________
ESTIMATION OF GENETIC PARAMETERS FOR MILK TRAITS IN

DIFFERENT LACTATIONS OF HOLSTEIN-FRIESIAN CATTLE USING
RANDOM REGRESSION MODEL
Trivunović S. 1, Štrbac Lj. 1, Šaran M. 1, Obad M.1,
Janković D.1, Bunevski Gj. 2, Radinović M. 1
Abstract: The aim of this study was the estimation of genetic parameters for milk traits
(milk yield, fat content and protein content) using random regression model. This model
allows evaluation of variability and correlation between different lactations. Each of
lactations is treated as separate trait and in relation to multiple trait model, a smaller
number of parameter are needed for evaluation. Data including 61.871 measurements of
Holstein-Friesian cows in Vojvodina calved between 2011 and 2016 year. The effect of
fixed factors (breed, year of calving, calving season, lactation in order and age) were tested
using the GLM in Statistica 13.0. Genetic analysis was performed in the WOMBAT
software by the REML. All factors tested showed a statistically significant effect on the
observed milk traits (P<0.01). The heritability of the milk yield ranged from 0.041 to
0.419, for the fat content from 0.389 to 0.490, for the protein content from 0.323 to 0.393
and increased with increasing age in all of the tested traits. Negative genetic and
phenotypic correlations for milk yield were calculated between the second with all the rest
age (except with third). High and positive genetic correlations were calculated from the age
of four to eight while all other correlations were medium and positive. For fat and protein
content genetic correlations were positive and high, while phenotypic correlations were
medium to high and also positive. Heritability estimates indicate that it is possible to
achieve genetic improvement. This can be achieved by selection, without the introduction
of genes from other populations.
Keywords: random regression model, milk traits, genetic parameters
1
Trivunović Snežana, PhD, full professor; Štrbac Ljuba, PhD, teaching assistant; Šaran Momčilo,
MSc, teaching assistant; Obad Milan, BSc, student; Janković Dobrila, PhD, associate; Radinović
Miroslav, MSc, associate; University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia;
2
Bunevski Gjoko, PhD, full professor, Ss. Cyril and Methodius University, Faculty of Agriculture
Sciences and Food, Skopje, R. of North Macedonia.
Corresponding author: Trivunović Snežana, email: snezana.trivunovic@stocarstvo.edu.rs
14
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EFFECT OF THE CRYOPRESERVATION ON THE ELEMENTAL

COMPOSITION OF RABBIT ENDOTHELIAL PROGENITOR CELLS
Vašíček J. 1,2, Baláži A.1, Tomková M.2, Miššík J.2, Tomka M.2, Chrenek P.1,2
Abstract: The aim of this preliminary study was to assess the possible changes in the
composition of selected elements (Na, K and Ca) within the rabbit endothelial progenitor
cells (rEPCs) triggered by their cryopreservation. Briefly, rEPCs were isolated and
cultured until the confluency about 90% for at least 2-3 weeks till passage 3 as described in
our previous studies. Then cells were harvested and counted using an EVETM automated
cell counter (NanoEntek, USA). An aliquot of cells (at least 4 x 107 cells) were washed
twice in Tris-HCl and the pellets were frozen at -20°C until the next processing. The rest of
cells (at least 4 x 107 cells) were frozen and stored in liquid nitrogen for at least 3 months.
Briefly, cells were resuspended in a pre-cooled (4°C) culture media containing 10%
DMSO (EMPLURA, Merck). Once aliquoted, cryovials were transferred into a “Mr.
Frosty” freezing container (Thermo Scientific Nalgene, Rochester, NY, USA), and stored
at -80°C for 24h. Cryovials were then transferred into liquid nitrogen for long-term storage
at -192°C. Frozen samples were then thawed as described previously. Thawed cells were
washed twice in Tris-HCl and the pellets were frozen at -20°C until the next processing.
Fresh and cryopreserved samples were then processed at once by their thawing and
mineralization in HNO3 solution. Mineralized samples were subsequently analyzed using
inductively coupled plasma optical emission spectrophotometer (ICP-OES - Thermo ICAP
7000 Dual) for the content of selected elements (Na, K and Ca) in fresh and cryopreserved
samples. Results were statistically evaluated using One-way ANOVA. Observed results
revealed increase in the content of Na and Ca and decrease in the content of K after
cryopreservation, although those changes were not significant. In conclusion,
cryopreservation of rabbit endothelial progenitor cells did not significantly alter their
elemental composition and hence the quality of these cells remains preserved.
Keywords: rabbit, endothelial progenitor cells, cryopreservation, macroelements, ICP-OES
1
Vašíček Jaromír, PhD., researcher; Baláži Andrej, PhD., researcher; Chrenek Peter, DrSc.,
professor; NPPC - Research Institute for Animal Production Nitra, Lužianky, Slovak Republic;
2
Vašíček Jaromír, PhD., researcher; Tomková Mária, PhD. student; Miššík Juraj, PhD., researcher;
Tomka Marián, PhD., researcher; Chrenek Peter, DrSc., professor; Slovak University of
Agriculture in Nitra, Faculty of Biotechnology and Food Science, Nitra, Slovak Republic.
* Research is supported by grants: APVV-14-0348 and VEGA 1/0160/18.
Corresponding author: Vašíček Jaromír, email: jaromir.vasicek@gmail.com
15
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CHANGES IN THE RELATIVE EXPRESSION OF SELECTED MARKERS

DURING THE CULTURE OF RABBIT ENDOTHELIAL PROGENITOR CELLS
Vašíček J. 1,2, Baláži A.1, Bauer M.1,3, Chrenek P.1,2
Abstract: The aim of this preliminary study was to assess the differences in phenotypic
expression among different passages of rabbit endothelial progenitor cells (rEPCs) using
qPCR analysis. Briefly, rEPCs were isolated and cultured as described in our previous
studies. The adherent cells in primary culture (P0) and each other 3 passages (P1-P3) were
cultured until the confluency about 90% for at least 2-3 weeks. Then they were harvested
and an aliquot of cells were reseed again as a next passage. The rest of cells were used for
qPCR analysis of selected rEPCs markers (CD133, CD146 and vWF) that were not
possible to be previously identified by flow cytometry. Total RNA isolation from rEPCs
and cDNA synthesis was performed as described previously. A qPCR was performed as
described previously with specific primers for each marker: CD133 (5'-3',
tcattccgatggaacagtca; atgaagttctgggcgtcatc, accession No. XM_017350447.1), CD146 (5'-
3', gacaatggcatcctggtctt; agtagctcgtggcgttcact, accession No. XM_008250510.2), vWF (5'-
3', cgagagcaagtggacattca; tggtacacgagggaaccatt, accession No. NM_001329088.1) and
beta-2-microglobulin (B2M) as a reference gene (5'-3', attcacgcccaatgataagg;
atcctcagacctccatgctg, accession No. XM_008269078.2). The amplification protocol was as
follows: initial denaturation at 95°C for 7 min followed by 30 cycles (B2M) or 40 cycles
(CD133, CD146 and vWF) of 95°C for 10 s, 60°C for 10s and 72°C for 10s. A relative
quantification of selected marker expression to reference B2M gene was calculated as
described previously. Results were statistically evaluated using One-way ANOVA. qPCR
analysis revealed stable relative expression of CD133 and CD146 markers during the
whole culture without any significant changes among the passages (P0-P3). However, the
relative expression of vWF had decreasing tendency over the culture, although the changes
among the passages were not statistically significant. These preliminary results indicated
the possible changes (vWF expression) in rEPCs phenotype during the long-term culture
and moreover the necessity of introducing another technique for further phenotypic cell
analysis in case of the unavailability of specific anti-rabbit antibodies for flow cytometry.
Keywords: rabbit, endothelial progenitor cells, culture, qPCR
1
Vašíček Jaromír, PhD., researcher; Baláži Andrej, PhD., researcher; Bauer Miroslav, PhD.,
associated professor; Chrenek Peter, DrSc., professor; NPPC - Research Institute for Animal
Production Nitra, Lužianky, Slovak Republic;
2
Vašíček Jaromír, PhD., researcher; Chrenek Peter, DrSc., professor; Slovak University of
Agriculture in Nitra, Faculty of Biotechnology and Food Science, Nitra, Slovak Republic;
3
Bauer Miroslav, PhD., associated professor; Constantine the Philosopher University in Nitra,
Faculty of Natural Sciences, Nitra, Slovak Republic.
* Research is supported by grants: APVV-14-0348 and VEGA 1/0160/18.
Corresponding author: Vašíček Jaromír, email: jaromir.vasicek@gmail.com
16
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THE INFLUENCE OF MOTHER'S AGE ON BIRTH WEIGHT OF LAMBS
Pihler I. 1, Zarubica B.1, Kučević D.1, Dragin S.1, Žarković I.1, Mekić C. 2, Lazić M.1
Abstract: It is considered that the primary potential for increasing the production of meat,
milk and wool is to increase reproductive activity of the sheep. This claim is based on the
fact that most of sheep breeds express seasonal sexual activity. This means that the interval
between two consecutive lambs is quite long. The vitality of lambs, and its survival, as
well as its further development depends on the weight at birth. The weight of the lambs at
birth plays a major role in achieving profitable production: the mortality decreases and
therefore the number of lambs in the herd increases, the craft of the primary herd is
accelerating, the costs of treating the diseased lambs are reduced, the conversion is better,
the growth is better, the better finishing weights of the lambs are achieved ,meat quality is
better, better utilization of farm capacity, healthy lambs faster and easier enter the puberty,
as the fact that lambs are born healthy, there are bigger chances for lambs to give healthy
offspring.
For the purposes of the research, there were analyzed 136 litter weights, obtained after the
estrus synchronization and the lambing of 116 sheep breeds of Ile de France. Five groups
of sheep were formed, which in the moment of mating were aged from 1 to 5 years old.
The weight of the lamb was measured at birth, at 1 month of age and at cessation of milk
sucking (at the age of 70 days). The minimum weight of lambs at birth is 3.1 kg, and the
highest weight on the birth of the lamb is 7.4 kg. The highest average birth weight is 4.877
kg (sheep at the age of three years) and the smallest 4.474 kg (sheep at one year old). The
lowest mean value of one month old is 11,164 kg (sheep at the age of three), while the
highest mean value is 12,180 kg (sheep at one year old). The lowest mean weight of lambs
at the age of 70 days is 20,508 kg from 4 years old sheep, while the highest mean weight is
24,044 kg from 5 years old sheep.
Keywords: birth, breed, lamb, sheep, weight.
1
Pihler Ivan, PhD, associate professor, Zarubica Baćo, MSc, teaching associate, Kučević Denis,
PhD, associate professor, Dragin Saša, PhD, associate professor, Žarković Ivan, MSc, associate,
Lazić Milenko, livestock engineer, University of Novi Sad, Faculty of Agriculture, Novi Sad,
Serbia;
2
Mekić Cvijan, PhD, professor; University of Belgrade, Faculty of Agriculture, Zemun, Serbia.
Corresponding author: Zarubica Baćo, email: baco.zarubica@stocarstvo.edu.rs
17
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THE IMPACT OF POMEGRANATE ON PRESENCE OF STEROID HORMONE

RECEPTORS IN OVARIAN CELLS
Baldovská S.1, Michalcová K.1, Kolesárová A. 1
Abstract: Cancer prevention via dietary agents is a quite promising target in biomedicine
and pharmacology. Current studies have suggested the extracts of all parts of the
pomegranate fruit, as promising chemopreventive agents, as they exhibit anti-
inflammatory, antiproliferative, and anticancer effects by modulating multiple signaling
pathways. Estrogens play a critical role in hormone-receptor-positive cancer development,
by binding to and activating the estrogen receptors (ER). Although the mechanism has not
been conclusively defined, progesterone is generally thought to mediate its protective
action through well-characterized nuclear progesterone receptors (PGR). In this study, the
ethanol extract of pomegranate peel was analyzed for its possible property as selective
modulator of ER and PGR, using the human ovarian granulosa cell line (HGL-5) and the
human ovarian carcinoma cell line (OVCAR-3) at the concentrations 0; 12,5; 25; 50; 100
and 200 µg/mL; for 24 h. The level of human estradiol receptor α (ERα) and human
progesterone receptor (PGR) was determined by the ELISA method. The ERα present in
HGL-5 cells was not significantly influenced (P≥0.05) after addition of pomegranate
extract compared to control cells. On the other hand, the ERα present in OVCAR-3 cells
was significantly decreased (P≤0.05; P≤0.001) after addition of the concentrations 100 and
200 µg/mL of the pomegranate extract. The levels of PGR present in HGL-5 cells were
significantly decreased (P≤0.01) after addition of the concentrations 100 and 200 µg/mL of
pomegranate extract in comparison to control cells but without significant influence
(P≥0.05) in OVCAR-3. In conclusion, estrogen receptor α (ERα) and progesterone receptor
(PGR) are two key proteins in the development of endocrine-responsive cancers. Our
findings demonstrate that pomegranate peel extract binds to and affects ERα and PGR in
human ovarian cells in vitro. However, the role of pomegranate in ERα- and PGR-
regulation in the cells remains unclear.
Keywords: pomegranate, ERα, PGR, ovarian cells, cancer
1
Kolesárová Adriana, PhD, professor; Baldovská Simona, MSc, PhD student; Michalcová
Katarína, MSc, PhD student; Slovak University of Agriculture in Nitra, Faculty of Biotechnology
and Food Sciences, Nitra, Slovak Republic.
*Research was supported by projects VEGA 1/0039/16, VEGA 1/0411/17, APVV-16-0170,
The Excellent scientific team “Animal Reproduction Center (CeRA)”, and Tatra bank
Foundation.
Corresponding author: Kolesárová Adriana, email: adriana.kolesarova@uniag.sk
18
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EXTENSITY OF HELMINTOSES OF SHEEP

IN BAČKA REGION OF SERBIA
Becskei ZS. 1*, Savić M.1, Ilić T.1, Sotiraki S. 2, Charlier J.3
Abstract: Worldwide, sheep production is affected by nematode parasites, a mjor cause of

disease and economic losses. Anthelmintic resistance is a growing concern for effective
parasite control in Europe and globally. In order to support the development of sustainable
parasite management strategies, combatting anthelmintic resistance and obtaining a
rationalized anthelmintic drug usage in Serbia, we initiated a survey on the prevalence and
variety of endoparasites in sheep. The current study included 61 small sheep flocks (up to
100 animals) located in 28 different villages in the Bačka region of Serbia. On the tested
households, the farmers applied at least one antiparasitic treatment in the last year. None of
the farms applied a coprological analysis or veterinary consultation before performing the
antiparasitic treatment. Prevalence and extensity of endoparasitic infections of sheep were
tested using 5 animals per farm (a total of 305 sheep), based on parasitological
examination of the gastrointestinal tract and liver at the slaughterhouse, and on qualitative
coprological methods of collected fecal samples from the rectum. The presence of
helminth eggs in all fecal samples was analyzed using the saturated sodium chloride
floatation technique, sedimentation techniques (Soulsby, 1986; Hansen and Perry, 1994;
Urquhart et al., 1996) and method by Euzeby (Euzeby, 1982). Identification and
determination of the parasite was done by morphometric analysis based on eggs and adult
parasites by the keys given by Soulsby (1977) and Euzeby (1982). Results showed that
68.8% of the tested sheep flocks were affected. Of all the tested sheep, the survey revealed
endoparasitic infections with roundworms (Trichostrongylus spp. - 52.1%,
Oesophagostomum spp. - 22.2%, Chabertia ovina - 12.8%, Nematodirus spp. - 11.5%,
Haemonchus spp. - 10.5%, Ostertagia spp. - 4.9%, Bunostomum spp. - 4.9%, Skrjabinema
spp. - 1.6% and Dictyocaulus filaria – 10.5%), tapeworms (Moniezia spp.- 41,0%) and
flukes (Dicrocoelium dendriticum - 18.0%). These results confirm the presence and variety
of helminth in sheep in Serbia. Furthermore, because the anthelmintic treatments were
done without preliminary diagnosis or using veterinary assistance, the emergence and
spread of anthelmintic resistance should be evaluated. Introducing the topic in farmer
discussion groups and implementing regulation for sustainable anthelmintic usage may be
prerequisites to improve parasite management approaches in Serbia.
1
* Becskei Zsolt, corresponding author, PhD, assistant professor,; Savić Mila, PhD, professor, Ilić
Tamara, professor, University of Belgrade, Faculty of Veterinary Medicine, Belgrade, Serbia;
2
Sotiraki Smaragda, PhD, Diplomate EVPC, Veterinary Research Institute Hellenic Agricultural
Organization DEMETER; Thermi, Greece;
3
Charlier Johannes, PhD, Diplomate EVPC, Kreavet, Kruibeke, Belgium.
*Research is a part of Cost Action 16230 COMBAR, supported by COST (European
Coperation in Science and Technology), and TR31085 Project of the Ministry of
Education, Science and Technological Development of Serbia.
19
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ANTIMICROBIAL SENSITIVITY PATTERN OF EGYPTIAN AVIAN

PASTEURELLA MULTOCIDA STRAINS
Wafaa A. Abd El-Ghany1*, Hanan A. Ahmed 2 and Ali Z. Qandoos1
Abstract: This work was designed to characterize Pasteurella multocida (P. multocida)
isolates from layers and breeders chickens flocks in Egypt with emphasis on in-vitro
antibiotic sensitivity and resistant pattern. Liver, heart, spleen and lungs were collected
aseptically from diseased birds suffered from respiratory manifestations, septicaemia, drop
in egg production and mortalities during the period from 2016-2017. Samples were
cultured on modified Das media for isolation of bacteria. Pure colonies of P. multocida
isolates were identified according to microscopic morphology and biochemical characters.
The isolated P. multocida were subjected to in-vitro antibiotic sensitivity test. Cultural
study revealed small glistering, grayish, mucoid and dew drop P. multocida colonies.
Microscopically, P. multocida isolates were Gram negative coccobacill. All the isolates
were positive for catalase, oxidase, indol production, nitrate reduction and H2S production
tests, while negative for methyl red, Voge’s proskaur, urease activity and gelatin
liquefaction tests. Moreover, they fermented glucose, fructose, mannose, mannitol,
sucrose, sorbitol and xylose without gas production but not ferment arabinose, inositol,
lactose, maltose, salicin, dulcitol and raffinose. Isolated P. multocida isolates was sensitive
to Ofloxacin, Tetracycline, trimethoprim/sulphamethoxazole, Penicillin, Chloramphenicol,
Norfloxacin, Azithromycin, and Erythromycin while resistant to Ampicillin and
Clindamycin. Intermediate sensitivity was observed for Cefoperazone, Gentamycin and
Streptomycin.
Key words: antibiotics, chicken, Egypt, P. multocida, susceptibility
1
Wafaa A. Abd El-Ghany, Professor of Poultry Diseases, Department Poultry Diseases, Faculty of
Veterinary Medicine, Cairo University, Egypt. Ali Z. Qandoos, Ph.D student, Department Poultry
Diseases, Faculty of Veterinary Medicine, Cairo University, Egypt.
2
Hanan A. Ahmed, Professor, Central Laboratory for Evaluation of Veterinary Biologics, Cairo,
Egypt.
*
Corresponding author: Wafaa A. Abd El-Ghany, email: wafaa.ghany@yahoo.com
20
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FRUIT BYPRODUCTS: A POSSIBLE MODULATORS OF OVARIAN

STEROIDOGENESIS IN VITRO
Kolesárová A. 1, Baldovská S.1, Michalcová K.1, Gálik B. 2
Abstract: Current progress in the field of pharmacology and biomedicine have enhanced
the significance of polyphenol-rich fruits, including grapes and pomegranates as a
nutritional food against various diseases. Grape pomace as a byproduct of winemaking and
pomegranate husk as a byproduct of pomegranate juice contains health-promoting
compounds with strong antioxidant, antibacterial and anticancer properties. In this study,
we determined the effect of the extract of grape pomace and the extract of pomegranate
husk at the concentrations 0; 12,5; 25; 50; 100 µg/mL (for 24 h) on the human granulosa
cells (HGL-5) in vitro. The aim was to evaluate the influence of these fruit extracts on the
cell viability and secretion of steroid hormones by the cells. The metabolic activity was
evaluated by AlamarBlueTM cell viability assay, the release of progesterone and 17beta-
estradiol was assayed by ELISA methods. The number of viable HGL-5 cells was not
significantly influenced (P≥0.05) at all used concentrations of grape pomace extract
compared to control cells. On the other hand, the level of viable HGL-5 cells was
significantly increased (P≤0.01) at the highest concentration of pomegranate husk extract.
The secretion of progesterone and 17beta-estradiol was significantly (P≤0.05) decreased at
the highest concentration of grape pomace extract. In addition, the all used concentrations
of pomegranate husk extract led to significant (P≤0.05; P≤0.01) decrease of the
progesterone secretion. Moreover, the level of 17beta-estradiol was significantly (P≤0.001)
increased at the concentration 50 and 100 µg/mL in comparison to control cells after
addition of pomegranate husk extract.
In conclusion, the study shows possible modulation of steroidogenesis by both, grape
pomace extract and pomegranate husk extract in human ovarian granulosa cells (HGL-5) in
vitro. The mechanisms of action are not yet fully discovered, however, the potential role of
fruit byproducts in the regulation of ovarian functions was demonstrated.
Keywords: grape, pomegranate, steroid hormones, granulosa cells
1
Kolesárová Adriana, PhD, professor; Baldovská Simona, MSc, PhD student; Michalcová
Katarína, MSc, PhD student; Slovak University of Agriculture in Nitra, Faculty of Biotechnology
and Food Sciences, Nitra, Slovak Republic;
2
Gálik Branislav, PhD, professor; Slovak University of Agriculture in Nitra, Faculty of
Agrobiology and Food Resources, Nitra, Slovak Republic.
*Research is supported by projects VEGA 1/0039/16, VEGA 1/0411/17, APVV-16-0170,
The Excellent scientific team “Animal Reproduction Center (CeRA)”, and Tatra bank
Foundation.
Corresponding author: Kolesárová Adriana, email: adriana.kolesarova@uniag.sk
21
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STAKEHOLDERS’ PERCEPTIONS OF KEEL BONE DAMAGE

IN LAYING HENS: EVIDENCE FROM SERBIA*
Rodić V. 1, Perić L.1, Đukić Stojčić M.1, Rezar V. 2, Relić R.3, Despotović J.1
Abstract: To meet citizens’ expectations and market demands the EU banned battery cages
in 2012. However, soon after transition to enriched cages and non-cage systems it was
clear that these systems are not rid of welfare problems (Widowskiet al., 2017; Đukić-
Stojčić et al., 2017). Consequently, discussion has begun about the need of a new
regulatory change, including reconsidering caging laying hens again (Guyonnet, 2018).
Keel bone damage (KBD) in laying hens is a welfare issue which attracts a growing
attention in the EU because of its high prevalence in permitted housing systems. Consensus
among researchers on the causes and influencing factors of KBD has not been reached yet
(Wilkins et al., 2011; Tarlton et al., 2013; Harlander-Matauschek at al., 2015). Likewise, it
is still the subject of scientific debate whether and to what extent KBD is painful (Nasr et
al., 2012; Harlander et al., 2015) and how it effects production and quality of eggs
(Heerkens et al., 2013). Nonetheless, there is a growing number of studies that point out
the severity of the problem and need for urgent solutions (Kappeli et al., 2011). This issue
is of particular importance for Serbia (and other non-EU countries) where battery cages are
not abandoned yet but will have to be if/when country joins the EU. A disadvantage of
being late in the adoption of welfare standards could be turned into an advantage, but only
if relevant regulation improves based on the EU experience, instead of blindly following
the same path. However, the question is who could/should drive the changes, since it is
hypothesized in this paper that current knowledge about KBD is very limited. The main
aim of the paper was to find out perceptions of key egg value chain stakeholders’
perception of KBD, in order to identify the driving force behind legislation change. For
this purpose, 547 respondents (farmers, poultry experts and citizens) have been surveyed.
The obtained results confirmed the initial hypothesis, that knowledge about the KBD is
generally very low. Particularly low is knowledge of general public (only 14% of
respondents know causes of KBD). The only driving force behind legislation change could
be poultry experts, who are somewhat better informed (34% know causes of KBD) than
citizens and farmers. If concern for hens’ welfare is genuine they should act in this
direction as soon as possible.
Keywords: stakeholders, perceptions, welfare, keel bone damage
1
Rodić Vesna, PhD, professor; Perić Lidija, PhD, professor; Mirjana Đukić Stojčić, PhD, professor;
Despotović Jelena, MSc, assistant, University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia
2
Vida Rezar, PhD, assistant professor, University of Ljubljana, Biotechnical Faculty, Ljubljana, Slovenia
3
Renata Relić, PhD, associate professor, University of Belgrade, Faculty of Agriculture, Zemun, Serbia
* The research is a part of the project #TR31033 funded by the Ministry of Education, Science and
Technological Development of the Republic of Serbia.
Corresponding author: Vesna Rodić; email: rodicv@polj.uns.ac.rs
22
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THE EFFECT OF NUTRITION IN THE FINISHING STAGE ON

BISON MEAT YIELD AND QUALITY SCORE
Božić A. 1, Gaško J. 2, Beuković M.,1 Beuković D.1 Plavšić M.1
Abstract: Quality of bison meat depends on numerous factors, which in terms of

significance, first place takes the nutrition. In a bison diet, aside from roughage and grain it
includes water, minerals, vitamins, concentrates and other supplements. When raising
bison, the most important is the method of nutrition in the final period for achieving better
performances and economic production. Manufacturers commonly use one of the
following two methods of nutrition in the final period: the first one is based on a rich diet
of numerous plants, hay and pellets, and the second method is based on the nutrition with
whole or crushed grain of cereal and concentrates. The goal of research was testing the
effectiveness of those two methods and their influence on the yield and quality score of
bison meat.
The experiment was conducted in the period of 2013 to 2014 in Grande Prairie Free
Range Bison in DeBolt, located in western Alberta, Canada. Feed manufactured by
Champion Feed Services, Ltd, Grande Prairie, Alberta, Canada, which we fed the bison,
have been tested at Central Testing Laboratory Ltd. Winnipeg, Manitoba, Canada, while
the evaluation of meat after the slaughter of bison has been conducted at Lacombe
Research Centre, Lacombe, Alberta, Canada. The obtained data were tested on two groups
of prairie bison whose diet differed in the finishing stage. The nutrition of the first group
was made of pelleted complete feed and hay, while the second group was fed crushed
grain, pelleted supplement and hay. The results indicate significant influence of a specified
type of feeding on meat yield and quality, regarding the achieved and expected evaluation
of the meat.
Keywords: bison, feeding, meat quality, oats, pellets - complete feed
1
Božić Aleksandar, PhD, professor, Beuković Miloš, PhD, professor, Beuković Dejan, PhD
assistant professor, Plavšić Miroslav PhD, professor, University of Novi Sad, Faculty of
Agriculture, Serbia;
2
Jaroslav Gaško,MS,Quality and Food Safety Specialist, Masterfeeds Inc. 1020 Hargrieve Rd.
London, Ontario,N6E 1P5, Canada.
Corresponding author: Aleksandar Božić, e-mail: aleksandar.bozic@stocarstvo.edu.rs
23
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UTILIZATION OF OLIVE BY-PRODUCTS IN NON-RUMINANT NUTRITION

Levart A.1, Rezar V. 1, Leskovec J. 1, Pirman T.1, Butinar B.2,
Bučar Miklavčič M.2, Podgornik M.2, Salobir J.1
Abstract: Olive (Olea europaea) growing and production of olive oil generate large
amounts of by-products, e.g. olive leaves and cake. Uncontrolled disposal of those by-
products (plant waste) on small areas can have negative impact on the environment. Olive
leaves and cake contain anti-nutritive components, such as lignin, tannins, and
polyphenols, which limit their use as a feedstuff. Those by-products could be useful source
of nutrients, such as fat and antioxidants for the animals. There is no research concerning
practical use of olive by-products in animal nutrition in Slovenia.
In the research project entitled “Utilization of olive growing by-products”, sampling (39)
and analysis of chemical composition (proximate analysis, detergent fibre, selected
minerals) of olive cake from Slovenian olive oil mills was conducted. Feeding trial on
broiler chickens (120 twenty-one day old Ross 308 broilers), not supplemented (CONT) or
supplemented with 5% or 10% of olive leaves (5OL, 10OL) or olive cake (5OC, 10OC)
was also performed.
Obtained results demonstrated high variability in chemical composition and nutrients
content of olive cake, which could be attributed mainly to processing technologies. On
average, olive cake contained 60 ± 14 g of crude protein, 407 ± 101 g of crude fibre, and
159 ± 42 g of crude fat per kg of dry mass. There were no differences in body mass, weight
gain, carcass weight, proportion of internal organs (hearth, liver, gizzard, gastrointestinal
tract), and carcass cuts (wings, thighs, breasts). The lack of pronounced negative effects of
olive leaves or cake on productive parameters suggest that both by-products could be
safely used as a feed ingredient in broiler nutrition, preferably in less intensive production
systems.
Keywords: olive cake, olive leaves, composition, broilers, feeding trial
1
Levart Alenka, PhD, teaching assistant; Rezar Vida, PhD, assistant professor; Leskovec Jakob,
MSc, PhD student; Pirman Tatjana, PhD, associate professor, Salobir Janez, PhD, full professor;
University of Ljubljana, Biotechnical Faculty, Department of Animal Science, Domžale, Slovenia;
2
Butinar Bojan, PhD, research associate; Bučar Miklavčič Milena, MSc, teaching assistant;
Podgornik Maja, PhD, research fellow; Science and Research Centre of Koper, Institute for
oliveculture, Koper, Slovenia.
Corresponding author: Alenka Levart, email: alenka.levart@bf.uni-lj.si
24
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COLORED SOYBEAN SEEDS: A NEW SOURCE OF FUNCTIONAL FEED IN

ANIMAL NUTRITION
Malenčić Đ.1, Miladinović J.2, Kiprovski B.2, Mikulić M.3, Hogervorst J.3, Prvulović D.1
Abstract: Food and feed of plant origin containing phytochemicals of great antioxidant
potential are called nowadays “functional”, which means that they beside nutritive traits,
feature also some medicinal properties. Plant phenolics show useful characteristics in
human and animal metabolism, such as antiinflamatory and even anticarcinogenic. They
posses antibacterial, antimutagenic and vasodilatory activities, as well. Acting as natural
antioxidants they lower the risk of atherosclerosis and cardiac illness. Thus, soybean based
feed enriched with polyphenols may be classified as functional feed in animal diet.
Content of total polyphenols, tannins, flavonoids and anthocyanins were evaluated in the
seeds of seven soybean varieties of different seed color. One yellow seed cultivar
represented a reference genotype. Isoflavone (phytoestrogen) content and composition was
determined using HPLC analysis on an Agilent model 1100, equipped with diode array
detector (DAD). Aglycons were quantified from three five-point regression curves (R ≥
0.9998) obtained using the corresponding standards (daidzein, glycitein and genistein). In
addition, antioxidant activity of seed extracts was evaluated by the 2,2-diphenyl-1-
picrylhydrazyl (DPPH) free radical scavenging activity assay.
A positive linear correlation between antioxidant activity and contents of total polyphenols
and anthocyanins was established. The highest antioxidant activity was observed in the
extracts of black and brown varieties, which also showed high levels of all polyphenol
classes examined. Yellow seed had the highest total isoflavone content (3.62 mg/g of dry
material). The highest concentration of total daidzein was determined in black seeds (>2.0
mg/g d.m.), and the highest total glycitein and genistein contents occurred in the yellow
cultivar (0.53 and 1.49 mg/g d.m., respectively). Varieties of black and brown seeds could
be of special interest due to large content of polyphenols (4.94-6.22 mg/g d.m.), especially
from the class of anthocyanins. Obtained data should enable the selection of genotypes rich
in natural antioxidants that could further be processed into functional feed in animal diet.
Keywords: colored soybean seeds, functional food, polyphenols, antioxidant activity
____________________
1
Malenčić Đorđe, PhD, full professor; Prvulović Dejan, PhD, assoc. professor; University of
Novi Sad, Faculty of Agriculture, Novi Sad, Serbia;
2
Miladinović Jegor, PhD, principal research fellow; Kiprovski Biljana, PhD, research associate;
Institute for Field and Vegetable Crops, Novi Sad, Serbia;
3
Mikulić Mira, PhD, assist. professor; Hogervorst Jelena, PhD, full professor; University of Novi
Sad, Faculty of Medicine, Novi Sad, Serbia.
Corresponding author: Malenčić Đorđe, email: malencic@polj.uns.ac.rs
25
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EFFECT OF LOW PROTEIN DIETS ON OCCURRENCE OF

FOOTPAD DERMATITIS IN BROILERS
Perić L. 1, Dublecz K. 2, Žikić D.1, Đukić Stojčić M.1, Pál L.2
Abstract: The study was conducted to evaluate the impact of low protein diets on the main
parameters of the litter quality and consequently on the occurrence of footpad dermatitis
(FPD) in broilers. The experiment was conducted at the Experimental farm of the
University of Novi Sad, Faculty of Agriculture. A total of 296 Ross 308 broilers were
allocated to two treatments with four replicates. The bedding material was pelleted straw
and was added in an amount of 4 kg/m2.
Birds were fed either a standard feed (C) or a 2% protein reduced feed (LP), containing the
same ileal digestible amounts of the first limiting amino acids (MET, M+C, LYS, THR,
VAL). The level of crude protein in starter feed (1-12 days) was 23% (C) or 21% (LP), in
grower feed (13-33 days) 21% (C) or 19% (LP) and in finisher feed (34-42 days) 19% (C)
or 17% (LP). The nitrogen content in feces, moisture content in the litter, pH of the litter
and the litter score were evaluated at the age of 4 and 6-weeks. At the same time, the
incidence of footpad dermatitis was evaluated by the method described by Eichner (2007).
Reduction of the crude protein content in diets significantly decreased (P<0.01) the
nitrogen content in feces (%) at 4 (0.95 vs. 0.79) as well as at 6 weeks of age (1.20 vs.
0.93). There was no significant effect of the low protein diets on the moisture content in
litter (52.17 (C) vs. 47.15 (LP) at 4 weeks and 50.77 (C) vs. 45.37 (LP) at 6 weeks of age).
The pH of the litter was not significantly influenced by dietary treatments. The FPD score
was significantly lowered in groups with LP diets both at 4 weeks of age (0.59 (C) vs. 0.32
(LP)) and 6 weeks of age (1.81 (C) vs. 0.91 (LP)). In conclusion, the results showed that
reduction of protein content in feed lowered the nitrogen content in feces and significantly
lowered the incidence of FPD in broilers.
Keywords: broilers, dermatitis, footpad, protein
1
Perić Lidija, PhD,professor; Žikić Dragan, PhD,professor; Đukić Stojčić Mirjana
PhD,professor; University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia;
2
Dublecz Károly, PhD,professor; Pál Laszlo, University of Pannonia, Georgikon Faculty,
Keszthely, Hungary.
Corresponding author: Perić Lidija, email: lidija.peric@stocarstvo.edu.rs
26
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INTESTINAL MORPHOLOGY AND VOLATIL FATTY ACIDS CONTENT IN

BROILER CHICKEN IN DEPENDANCE OF ADDITION OF OLIVE LEAF OR
MARIGOLD EXTRACTS IN N-3 PUFA ENRICHED DIET
Pirman T. 1, Levart A.1, Rezar V.1, Vrecl Fazarinc M. 2, Salobir J.1
Abstract: Experiment was conducted to investigate if there is any beneficial effect of

dietary polyunsaturated fatty acids (linseed oil) and two potential natural antioxidants
supplementation, olive leaf extract (Olea europea) and marigold petal extract (Calendula
officinalis) on the small intestine morphology and levels of volatile fatty acid (VFA) in
intestinal content of broiler chicken.
1-day-old male broiler chickens, Ross 308, were housed in a deep litter system. First 21
days animals received started diets, after that, until slaughter, on 39th day, finisher diet,
enriched with 7 % of linseed oil. They were randomly divided into 3 groups of 15 animals
in each. Control finisher diet (Cont) was not supplemented, while both experimental diets
were supplemented with olive leaf (Olive) or with marigold petal (Marigold) extract.
Histological structure (villus height and crypt depth) of duodenum, jejunum and ileum was
analysed by histomorphometric analysis and the analysis of VFA in the small intestine and
colon content was performed by the gas chromatography.
The addition of extracts had no effect on performance of broilers. The variability inside the
groups were rather high. Olive leaf extract increased the level of ethanoic acid in intestinal
content, but only in colon content the difference was significant. On the other hand,
marigold petal extract decreased the crypt depth in duodenum of broiler chicken. It could
be concluded that the addition of extracts had no major effect on the fermentability or
morphology of the intestinal tissues.
Keywords: Olea europea, Calendula officinalis, broiler chicken, intestine morphology,

VFA
1
Pirman Tatjana, PhD, associate professor; Levart Alenka, PhD, assistant; Rezar Vida, PhD,
assistant professor, Salobir Janez, PhD, full professor; University of Ljubljana, Biotechnical
Faculty, Department of Animal Science, Domžale, Slovenia;
2
Vrecl Fazarinc Milka, PhD, full professor; University of Ljubljana, Veterinary Faculty, Institute
of preclinical sciences, Ljubljana, Slovenia.
Corresponding author: Pirman Tatjana, email: tatjana.pirman@bf.uni-lj.si
27
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EFFECT OF PHENOLOGICAL PHASE ON FLORISTIC COMPOSITION

AND BIOMASS QUALITY OF LOWLAND PASTURE
Radonjić D. 1, Marković B.1, Marković M.1, Stešević D. 2, Đokić M.1, Jokanović O.1
Abstract: Natural pastures in Montenegro cover poorer land, inadequate for intensive
exploitation. However, they are, together with natural meadows, the most important forage
source. The aim of this study was to estimate biomass chemical and floristic composition
on Agrostis stolonifera semi-natural grassland in the vicinity of the Podgorica, in early
vegetative, late vegetative and reproductive phase. Data on floristic composition and
structure of plant communities were obtained by using the standard Braun-Blanquet
methodology in plant vegetative phase. The samples of forage were collected according to
procedure AS-1064 (2012), and chemical analyzes were done according to AOAC (2000).
The pasture community consists of 52 species, which belong to 19 families. In the
taxonomical spectrum of families dominant was Poaceae (23 %), then Asteraceae (11.5%),
Cyperaceae (13.4%), Fabaceae (9.6%) Juncaceae (5.7%) and Lamiaceae (5.7). The
presence of the other families was much lower. Group of dominant species consists of:
Agrostis stolonifera, Alopecurus rendlei, Anthoxanthum odoratum, Genista tinctoria,
Gratiola officinalis, Lotus tenuis, Lythrum salicaria, Ononis spinosa. The floristic
composition varied considerably. The first aspect of flora (April) is characterized by 40
species. In the second floristic aspect (May) 46 species were reported and in the third
(June) aspect 42 species.
Chemical composition of the grassland biomass has been changed along the maturity phase
of biomass. A significant increase through phonological phases in the content of dry matter
(19.59 to 40.19 g/kg DM) and crude fibers (20.9 to 29.7 g/kg DM) and a decrease in crude
protein content (17.4 to 10.3 g/kg DM) were determined. According to the floristic
composition and biomass quality, this lowland pasture can be qualified as a moderate to
good quality.
Key words: floristic composition, pastures, phenological phase, biomass quality.
1
Radonjić Dušica, MSc, assistant; Marković Božidarka, PhD, professor; Marković Milan, PhD,
professor, Jokanović Olga, PhD student, Milena Đokić, MSc, assistant, Biotechnical Faculty,
University of Montenegro, Podgorica, Montenegro;
2
Stešević Danijela, PhD, professor; Faculty of Science and Mathematics, University of
Montenegro, Podgorica, Montenegro.
Corresponding author: Dušica Radonjić; email: dradonjic5@gmail.com
28
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THE INFLUENCE OF OLIVE LEAVES (OLEA EUROPEA) OR MARIGOLD

(CALENDULA OFFICINALIS) EXTRACTS ON OXIDATIVE STATUS OF
BROILERS FED N-3 PUFA ENRICHED DIETS
Rezar V. 1, Levart A.1, Pirman T.1, Nemec Svete A. 2, Kompan R.1, Salobir J.1
Abstract: High intake of PUFA in chicken diets increases the requirement for antioxidants.
Recent attempts to find novel bio-efficient antioxidants have focused particularly on
antioxidants rich plants like olive (Olea europea) and marigold (Calendula officinalis).
Olive leaves can be regarded as a particularly rich source of phenolic compounds known to
have antioxidant activity acknowledged also by EFSA. Calendula has also been reported to
have many pharmacological properties, including antioxidative.
A total of 45 1-day-old male broiler chicks Ross 308, were housed in a deep litter system
for 39 days. Broilers were divided into 3 groups and fed diets supplement with 7% of
linseed oil, and not supplemented (Cont) or supplemented with olive leave (Olive) or
marigold petal extracts (Marigold). Concentration of malondialdehyde (MDA) and vitamin
E in plasma, antioxidative capacity of water- and lipid- soluble antioxidants, SOD and
GPx, and liver enzymes in serum were measured. Also the rate of lymphocyte DNA
damage was assessed.
The results showed no effects on body weight gain and feed conversion. Olive leaves
extract significantly decreased MDA concentration in plasma for 25%, the 17% reduction
in marigold supplemented group was only numerical. The supplementation of marigold
extract decreased DNA damage for 5%. Both extracts had no effect on other measured
markers of oxidative status in blood. The results showed that extracts supplementation
used in the study had limited beneficial effect on oxidative status of broilers.
Keywords: oxidative status, linseed oil, Olea europea, Calendula officinalis, broilers
1
Rezar Vida, PhD, assistant professor; Levart Alenka, PhD, assistant; Pirman Tatjana, PhD,
associate professor, Rok Kompan, PhD student, Salobir Janez, PhD, full professor; University of
Ljubljana, Biotechnical Faculty, Department of Animal Science, Domžale, Slovenia;
2
Nemec Svete Alenka, PhD, associate professor; University of Ljubljana, Veterinary Faculty,
Clinic for Surgery and Small Animals, Ljubljana, Slovenia.
Corresponding author: Rezar Vida, email: vida.rezar@bf.uni-lj.si
29
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GASTROINTESTINAL PARASITES OF WILD BADGER (MELES MELES) AT

MEDVEDNICA NATURE PARK – PRELIMINARY RESULTS
Bujanić, M.1, Martinković, F.1, Štimac, I.1, Škvorc, N.1, Konjević, D. 1
Abstract: Badger (Meles meles) is autochthonous game species in Croatia from the family
of Mustelidae. Despite being carnivore, badgers is in fact omnivorous and opportunistic
species, whose diet is mainly composed of plants, fruits, arthropods and invertebrates.
Whole carcasses of badgers were collected as part of wildlife diseases monitoring at the
area of Medvednica Nature Park. In total 13 animals were analysed, of them 8 males and 5
females. Following the necropsy, whole digestive system was extracted from each animal,
incised with special scissors and content was flushed with water through sieves with
different mesh size. Observed parasites were stored in Falcon tubes with alcohol, while
remaining sediment was collected separately and analysed under microscope. Parasites
were identified using morphological keys. Prevalence of positive animals was 92.3%, of
them 7 males were positive (P=87.5%) and all females (P=100%). One animal was parasite
free. We have determined 5 parasite species, including 4 cestode species (Atriotaenia
incisa, Taenia sp., Diphyllobothrium sp. and Mesocestoides sp.) and 1 nematode species
(Uncinaria criniformis). Cestodes A. incisa was determined in 10 animals (P=76.9%),
Taenia sp., Mesocestoides sp. and Diphyllobothrium sp. were found in 2 animals each
(P=15.4%). U. criniformis was determined in 11 animals (P=84.6%). Obtained preliminary
results offer insight into parasitic fauna of the gastrointestinal system of badgers at
Medvednica Nature Park. Some of determined parasites are potentially zoonotic pathogens.
Keywords: badger, gastrointestinal system, cestodes, nematode, Madvednica Nature Park
1
Bujanić, Miljenko, PhD candidate; Martinković, Franjo, PhD, assistant professor; Štimac, Iva,
PhD candidate; Škvorc, Nikolina, student; Konjević, Dean, PhD, DECZM, associate professor,
University of Zagreb Veterinary Faculty, Zagreb, Croatia.
* Supported by project Wildlife Health and Zoonotic Potential at Medvednica Nature Park.
Corresponding author: Martinković Franjo; email: franjom@vef.hr
30
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FASCIOLOIDOSIS AND FASCIOLOSIS IN WILD BOAR (SUS SCROFA L.) ON

HISTOLOGICAL LEVEL
Šoštarić-Zuckermann, I.-C.1, Škvorc, N.1, Bujanić, M.1, Konjević, D. 1
Abstract: Fascioloidosis and fasciolosis are parasitic diseases of different species caused
by non-native trematode Fascioloides magna and autochthonous liver fluke Fasciola
hepatica. F. magna is parasitizing in the liver parenchyma within so called pseudocysts,
while F. hepatica invades bile ducts. Wild boar liver samples were collected during regular
hunting operations on the area of Sisačko-moslavačka County. Livers were analysed
macroscopically, sectioned at 2 cm thick layers and submitted for histological analysis
(H&E, Van Gieson). Samples of faeces were analysed using standard coprological
flotation and sedimentation techniques. Gross lesions included dark stained pigmented
areas and pseudocysts (fascioloidosis), and dilated bile ducts filled with yellowish fluid
and trematodes (fasciolosis). Flukes were identified using morphological keys.
Coprological analysis confirmed presence of only F. hepatica eggs. Microscopic findings
in the case of F. magna were characterized by migratory pathways filled with a mixture of
necrotic debris, blood and iron-porphyrin pigment, while the main findings in the case of
F. hepatica were proliferative and eosinophilic cholangitis. In one section an adult F.
hepatica fluke was visible. Wild boar represents definitive host for F. magna characterized
as dead-end host. The comparative histological analysis of livers infected with non-native
and native parasite offers insight into host-parasite interactions and could make a
contribution to understanding of wild boar reaction to infection with Fascioloides magna.
Keywords: wild boar, Fascioloides magna, Fasciola hepatica, histological analysis
1
Šoštarić-Zuckermann, Ivan-Conrado, PhD, assistant professor; Škvorc, Nikolina, student;
Bujanić, Miljenko, PhD candidate; Konjević, Dean, PhD, DECZM, associate professor, University
of Zagreb Veterinary Faculty, Zagreb, Croatia.
*Supported by Croatian Science Foundation Grant No. 8963 – Host-parasite interaction:
relation of three different host types on infection with Fascioloides magna.
Corresponding author: Konjević, Dean, email: dean.konjevic@vef.hr
31
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UNTREATED BEE COLONIES AND THEIR DEVELOPMENT IN

THE COURSE OF A YEAR
Nedić N. 1, Vojt D.1
Abstract: Over a last decade bee-keeping has experienced significant losses as a

consequence of a complex syndrome of colony collapse disorder. In the effort to preserve
natural biodiversity of bees and improve both their traits and selection the European Union
(EU) has undertaken some steps to improving in several directions within the project
entitled ”Sustainable Management of Resilient Bee Populations“. Within the working
package 6 of this project a performance testing protocol of bee colonies has been
developed which was supported by the project and applied in purposes on apiares
throughout a whole Europe.
On the apiary of the Agricultural Faculty in Belgrade, during 2017 and 2018, a trial was
carried out in the three groups of sister queen bees originating from different geographical
localities and breeders. The objective of the trial was to determine whether there are any
differences in monitored traits of different sister queen bees for the purpose of selecting
best queen bees for further propagation.
In the beginning of investigation a standard bee swarms were formed into which queen
bees were added later and all through to the end of the test these colonies were not treated
against varroa mite. Quantity of bees varied from 3.33 to 18.06 frames, and broods from
2.83 to 8.17 frames. Hygienic behavior was examined by use of pin-test in six repetitions.
The best hygienic behavior of 72.22% was exhibited by queen bees from the local apiary
while in the other two groups it accounted for 61.61 and 66.44 %, respectively. Presence of
varroa mite was controlled in four repetitions by use of powder sugar test and a
significantly higher presence of this parasite was determined in the second group.
Tests applied according to performance testing protocol were simple and efficient to use
and indicated the development of untreated bee colonies and existence of differences
between them on the basis of which a selection of best individuals could be performed.
Keywords: honey bee, breeding, production characteristics
1
Nedić Nebojša, PhD, Associate Professor; Vojt Denis, BSc; University of Belgrade, Faculty of
Agriculture, Belgrade, Serbia.
* We are grateful for the support by the Sustainable Management of Resilient Bee Populations –
SMARTBEES FP7‐KBBE program.2013.1.3‐02 / WP6 and to colleagues Prof. Dr. Kaspar
Bienefeld, Dr. Ralph Buchler, dr Aleksandar Uzunov; and the support by programme III 46008.
Corresponding author: Nebojša Nedić; email: nedicn@agrif.bg.ac.rs
32
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THE EFFECT OF HEN AGE OF ON THE QUALITY CHARACTERISTICS OF

TABLE EGGS
Đukić Stojčić M. 1, Perić L.1
Abstract: Very important issue for the consumers and for the processing industry is the
internal and external quality of table eggs. It is well known that the internal quality of eggs
starts to decline as soon as they are laid by hens. Different factors have a significant impact
on the internal and external quality characteristics of eggs, and one of them is age of laying
hens. The aim of this study was to evaluate the effects of laying hens age on the quality of
table eggs. Eggs from 49 and 72- wk-old Lohmann Brown- hens were sampled. In a sample
of 30 eggs internal and external egg quality parameters included were: egg weight,
cleanness of egg, shell color, shape index, shell thickness and shell weight, albumen
height, yolk height, yolk index, Haugh Unit (HU), yolk color and USDA.
The shape index, albumen height, and Haugh unit were greatly influenced by the age of
laying hens. Statistically significant effect of hens age was found on shape index (P<0.05),
albumen height (P<0.01), and HU (P<0.01). Eggs from young hens had higher shape
index compared to the eggs of older hens. Significant negative effect of hens age was on
albumen height and HU. Both parameters were higher in eggs from the younger lying hens
compared to the older ones (9.8 mm vs. 8.22 mm for albumen height; 114.85 vs. 88.81 for
HU).
Generally it can be concluded that it is crucial for the production of eggs of high quality to
understand the range of factors that affect internal and external egg quality characteristics.
In this study the results indicated that important internal egg quality characteristics
decrease by hen age.
Keywords: laying hens, egg quality, age
1
Đukić Stojčić Mirjana, PhD, full professor; Perić Lidija, PhD, full professor, University of
Novi Sad, Faculty of Agriculture, Department of Animal Science Novi Sad, Serbia.
Corresponding author: Mirjana Đukić Stojčić; email: mirjana.djukicstojcic@stocarstvo.edu.rs
33
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SENSORY CHARACTERISTICS OF KUČI CHEESE

Jokanović O. 1, Marković B.1, Mirecki S.1
Abstract: The physical, chemical, microbiological and sensory analysis of the food
products is used for the quality evaluation. But, lately, the sensory properties of food
products have become increasingly important. Sensory analysis is usually used as a tool to
improve the sensory quality of products and to achieve their standard quality. Cheese is
milk product characterized by a wide range of sensory properties that directly influence its
consumption. During ripening of cheese numerous chemical, biochemical, physical and
microbiological changes occur, that directly contribute to its sensory properties. Thus,
sensory analysis of cheese as a final product is used for its quality evaluation. The subject
of this paper is the sensory evaluation of Kuči cheese, produced in the Kuči area, north
from the Montenegrin capital of Podgorica with surrounding mountains that have natural
conditions for sheep and cattle production. The most of milk is used for production of
traditional Kuči cheese, that is full fat, white brine cheese. The ripening in the brine gives
to cheese specific sensory properties and possibility to be stored for a longer period,
different from other white brined cheeses produced in Montenegro.
The paper presents the results of sensory analysis of the three types of cheese: a) cheese
made of cow’s milk; b) ewe’s milk; and c) mixed milk (cow’s + ewe’s). The following
characteristics: taste, smell, consistency and color, outer appearance and cross section were
evaluated. The cheese is graded from 1 to 5 points. There are five categories of quality and
quality ranges from "unacceptable" to "excellent". The sensory analysis was performed
using scoring system in the sensory evaluation of cheese quality according to Ritz (1991).
The results of sensory analysis showed that all three types of cheese had good quality. The
best-rated type of cheeses is sheep's milk cheese, got 18.5 points of maximum 20, what
classified it as cheese with extraordinary sensory properties, especially when it comes to
tastes and cross-sectional appearance. The cheese made from mixed milk obtained 17.63
points, that classified it to the group of high-quality cheese while the cow's cheese had
17.08 points, classified also as high-quality cheeses.
According to the obtained results, it could be concluded that Kuči cheese has specific
stronger, a little bitter taste, but typical for this traditional –autochthonous cheese type. As
a conclusion, Kuči cheese is a part of cultural heritage of this area of Montenegro that
should be protected, standarised and better valorised.
Keywords: sensory characteristics, cheese, ewe’s and cow’s milk
1
Jokanović Olga, MSc; Marković Božidarka, PhD, professor; Mirecki Slavko, PhD, professor,
Biotechnical Faculty, University of Montenegro, Podgorica, Montenegro.
Corresponding author: Olga Jokanović; email: jokanovicolga@gmail.com
34
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BASIC BIOLOGICAL VALUE OF SOME TYPES OF CANNED FISH

Marković G. 1, Mladenović J.1, Pantović J.1
Abstract: Fish meat is an important source of valuable biological substances, primarily proteins
and fats. Four random samples of canned marine fish available on the Serbian market were used for
analysis, i.e. Atlantic mackerel in sunflower oil (manufactured by “Ocean Fish”, Bulgaria), herring
in sunflower oil (“Karavela” Ltd, Latvia), sardines in sunflower oil (“Eva” Podravka, Croatia) and
tuna in olive oil (“Thai Union Manufacturing”, Thailand).
Drained fish meat was analysed for total protein content by Kjeldahl method and lipid content by
Soxhlet method. The average protein content in fish samples was 21.34%, with levels ranging
between 18.59 (sardines) to 24.17% (Atlantic mackerel). Lipid content was 20.47% on average,
with a wide range of 9.42% (sardines) to 35.20% (herring). The lower protein and fat levels in
sardines suggest their lower nutritional importance compared to the other fish meat samples. The
high contents of protein and, especially, fats in pelagic fish from cold seas confirm their highly
beneficial role in human diet and health. The observed differences between samples are dependent
on fish species and fish canning technology used.
The analysis of some types of canned marine fish present on the Serbian market shows satisfactory
levels of total protein and fats. Complex evaluation of canned fish quality requires further analysis
for the presence of heavy metals, pesticides, detergents, mineral oils and other potential pollutants.
Keywords: protein, lipid, content, canned marine fish
1
Marković Goran PhD, Associate Professor; Mladenović Jelena, PhD, Assistant Professor;
Pantović Jelena, Assistant, University of Kragujevac, Faculty of Agronomy, Čačak, Serbia.
*The research was financially supported by Project TR31011 of the Ministry of Education, Science
and Technological Development of Serbia.
Corresponding author: Marković Goran, email: goranmsv@kg.ac.rs
35
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HYGIENIC QUALITY OF WHITE CHEESE PRODUCTION IN KRALJEVO

REGION
Mirković M. 1, Mirković N. 2, Ilić V. 3, Puđa P.1, Miočinović J.1,

Paunović D.1, Radulović A.1
Abstract: According to a joint report by the World Health Organization (WHO) and the
Food and Agriculture Organization (FAO) due to the use of unsafe food and water, over 2
billion people fall ill in the world annually. Diseases caused by different contaminated food
(Foodborne diseases) often have a fatal outcome and they occur in all countries over the
world. Today modern food safety system is based on the hygiene of the production process
and the implementation of the Good Manufacturing Practice (GMP), Good Hygiene
Practices (GHP) and HACCP. Cheeses have an important role in the nutrition of people
around the world, but it is also a suitable environment for the growth of microorganisms.
The most common problem in the production of white cheeses is the presence of
Escherichia coli and coagulase positive staphylococci, which indicate the level of the
hygiene of the production process. In this study, their presence was investigated in 90
samples of cheese from 3 craft dairy plants from Kraljevo region. For the enumeration of
Escherichia coli method SRPS ISO 16649- 2: 2008, and coagulase-positive staphylococci
method SRPS EN ISO 6888- 1: 2009 were used. In the same period, analyses of hygiene of
work surfaces (from 3 surfaces every 7 days) were carried out in total of 36 swabs, by the
reference method ISO 18593: 2004. The results of the analyses have led to data which
showed that from a total of 90 samples of cheese 3 samples were estimated as
unsatisfactory, 4 as acceptable and 83 as satisfactory when it comes to E.coli, while
coagulase-positive staphylococci were not recorded in any sample, suggesting a high level
of hygiene of the production process. From a total of 36 swabs taken from 3 work surfaces,
in only one swab E.coli was recorded, the coagulase-negative staphylococci were recorded
in 6 swabs, while 30 swabs were sterile.
In conclusion, these results showed that GMP, GHP and the frequent monitoring and
control of relevant parameters are preconditions in achieving hygienic safety in accordance
with the HACCP concept.
Keywords: cheeses, hygiene, swab, E. coli, coagulase-positive staphylococci
1
Mirković Milica, PhD, docent; Puđa Predrag, PhD, professor; Miočinović Jelena, PhD,professor;
Paunović Dušanka, MSc, researcher; Radulović Ana, BSc, master student, University of Belgrade,
Faculty of Agriculture, Zemun, Serbia;
2
Mirković Nemanja, PhD, scientific associate; Institute for Molecular Genetics and Genetic
Engineering, University of Belgrade, Serbia;
3
Ilić Vladimir, MSc, lecturer; High Medical School, Belgrade, Serbia.
Corresponding author: Mirković Milica, email: petrusicm@agrif.bg.ac.rs
36
_______________________________________________________________________________
HYGIENIC AND HEALTH SAFETY OF FRESH CHICKEN MEAT

Radulović, Z. 1, Mirković, M.1, Mirković, N. 2, Paunović, D.1
Abstract: Contaminated food and water often could have a fatal outcome, affecting all
countries in the world. The biological safety of chicken meat is gaining great attention in
both public health and food chain. Application of effective measurements for the reduction
of biological hazards, as well as Good Manufacturing Practice (GMP), Good Hygiene
Practices (GHP) and HACCP could lead to the safe and quality chicken meat.
Fresh chicken meat is suitable for the growth of many harmful microorganisms. However,
Salmonella sp. and Campylobacter sp. represent microorganisms that most affect on safety
of chicken meat. These microorganisms are included in the regulative for food safety and
hygiene of Republic of Serbia. The aim of this study was to determine the
presence/absence of Salmonella sp. and Campylobacter sp. from chicken carcasses in meat
industry during period of one year. During one year, 723 samples of chicken carcasses
were analyzed for the detection of Salmonella sp. using referent method ISO 6579-1:2017,
as well as ISO 10272-2:2017 for the detection of Campylobacter sp. Furthermore, hygiene
of work surfaces was conducted by swab sampling according to the method ISO
18593:2010, Enterobacteriaceae by ISO 21528-2:2009, Salmonellae sp. by ISO 6579:2008
and total aerobic microorganisms account by ISO 4833:2008 method.
Obtained results showed that Salmonella sp. was isolated from 78 samples of chicken
carcasses (10.79%), the highest presence of Salmonella sp. was determined in October
(38.64%). Campylobacter sp. was isolated from 284 samples (39.28%) mainly during
warmer months. From 59 swabs taken from different work surfaces and accessories, the
high total aerobic microorganism account was determined in 24 samples,
Enterobacteriaceae was detected in 5 samples and the presence of Salmonella sp. was
detected in two samples.
Obtained results could be used for risk assessment, with the idea that more adequate
conception of critical checkpoint and corrective measures should be taken to reduce or
eliminate the risk.
Keywords: chicken meat, Salmonella sp., Campylobacter sp., food safety, hygiene
1
Radulović Zorica, full professor; Mirković Milica, assistant professor; Dušanka Paunović, PhD
student, University of Belgrade, Faculty of Agriculture, Belgrade, Serbia;
2
Mirković Nemanja, Research Associate, University of Belgrade, Institute of Molecular Genetics
and Genetic Engineering, Belgrade, Serbia.
Corresponding author: Zorica Radulović, email: zradulovic@agrif.bg.ac.rs
37
_______________________________________________________________________________
BASIC INDICATORS OF LIVESTOCK PRODUCTION IN SERBIA

Bošnjak D. 1, Rodić V.1, Vukelić N. 1
Abstract: Developed agriculture implies significant share of livestock in the total

agricultural production. However, in Serbia, livestock share in the value of annual
agricultural output is rather low. In the period between 2008-2017 it was on average 31%.
Considering that in the 1970s this share in Serbia was about 50%, while in the developed
countries it goes up to 70% (Lučić et al., 2001) it is clear, and numerous studies confirm
(Rodić et al., 2007; Bošnjak and Rodić, 2008; Popović et al., 2010; Bošnjak et al., 2018),
that currently available natural conditions in Serbia are underutilized, at least when
livestock production is in question. In this paper, authors analyse the basic indicators of
livestock production in Serbia in the period 2008-2017 with the main aim to determine the
level and dynamics of the achieved production results in this sector. For the realization of
the set goal, the public data of the Statistical Office of the Republic of Serbia were used.
Estimation of livestock production capacity has been done based on the number of animals
and livestock units (LSU), while the productivity has been analyzed based on the
production of meat, milk, wool and eggs, as well as their quantities per animal, hectare and
per capita. The obtained results indicate that during the observed period the number of
animals decreased in all observed species, except sheep (average annual rate: cattle -
1.78%, pigs -2.31%, goats -4.77%, sheep 0.67% and poultry -0.56%). Also, the number of
LSU decreased (-1.38%). Consequently, the number of animals per hectare and per capita
in all types of livestock other than sheep was lower in 2017 than it was in 2008. The total
production of beef decreased, while the production of pork, poultry and lamb had a
growing tendency. The production of cow and goat milk decreased while the production of
sheep's milk had a positive trend. Milk productivity increased to 3,123 liters per cow, and
75.8 liters per sheep. The total production of eggs increased slightly while the number of
eggs per hen increased significantly (about 63%). The positive trends also characterized
wool production. Based on the obtained results one can conclude that in the observed
period all livestock enterprises, except for the ones for cattle breeding, had increasing
tendencies. However, the existing values of the observed indicators still lag behind those
achieved in developed countries with similar natural conditions.
Keywords: livestock production, indicators, Serbia
1
Bošnjak Danica, PhD, professor; Rodić Vesna, PhD, professor, Vukelić Nataša, PhD, assistant
professor, University of Novi Sad, Faculty of Agriculture, Novi Sad, Serbia;
* This research is a part of the projects #OI179028 and #TR31033 funded by the Ministry of
Education, Science and Technological Development of the Republic of Serbia.
Corresponding author: Vesna Rodić; email: rodicv@polj.uns.ac.rs
38
_______________________________________________________________________________
ANIMAL PRODUCTION EMPLOYMENT IN THE SLOVAK REPUBLIC

Chrenekova M. 1, Kubovicova E. 2
Abstract: Livestock production is a leading sector of agricultural production of countries

with a developed market economy. The aim of livestock production is, in addition to the
production of nutritional commodities, conservation of genetic resources, and production
and use of renewable energy sources. The importance of animal husbandry lies in
stabilizing soil fertility, landscaping and maintaining rural employment. There have been
significant changes in the Slovak agriculture over the last 30 years. This fact is reflected in
development of rural areas.
The aim of the paper is to analyze selected indicators of livestock production in the Slovak
Republic in the period 1993-2017. The subject of research is livestock status, productivity
and employment in livestock production. Methods of descriptive statistic were used.
Datasets of the Statistical Office, and Reports on Agriculture and Food Sector in the
Slovak Republic in the period 1993-2017 were used as basic sources of information for the
research.
In Slovakia, the share of agriculture in gross domestic product and employment in this
sector decreased significantly. The number of cattle dropped almost to one quarter, pigs to
one fifth, and sheep to almost half in the reviewed period. As a result, total livestock
production has fallen by almost 25%. The number of employees in agriculture has dropped
to almost one-seventh and in animal production even more. Employment in agriculture (%
of total employment) in Slovakia was reported at 2.9 % in 2017. However, thanks to the
introduction of new technologies in animal and plant production, production efficiency has
been increased.
One of the problems of Slovak rural areas is a lack of jobs. We suggest, that, among other
factors, development of livestock production would stabilize the Slovak rural economy.
Keywords: agriculture, animal production, employment, rural development, the Slovak

Republic
1
Chrenekova Marcela, PhD; Slovak University of Agriculture, Faculty of European Studies and
Regional Development, Nitra, Slovakia;
2
Kubovicova Elena, PhD; National Agricultural and Food Centre, Research Institute for Animal
Production, Nitra, Slovakia.
* The study was financially supported by the Slovak Research and Development Agency
(No. APVV-17-0124).
Corresponding author: Chrenekova Marcela, email: marcela.chrenekova@uniag.sk
39
_______________________________________________________________________________
ENDOSCOPIC STUDY OF UPPER RESPIRATORY

TRACT DISORDERS IN HORSES
Sabev S. 1
Abstract: Anomalies of the upper respiratory tract are common problem in sport horses and
often lead to processes of obstruction resulting in poor athletic performance and respiratory
noise during exercise.
The aim of the present study was to explore by endoscopic examination the upper
respiratory tract in problematic horses showing different degree of respiratory distress or
abnormal respiratory noise during exercise and to clarify the cause(s) for these
abnormalities.
After careful visualization of anatomical structures of the upper airway in all affected
animals were documented various anomalies. The most commonly diagnosed pathology
was associated with a movement of the larynx (recurrent laryngeal neuropathy) and dorsal
dislocation of soft palate (DDSP).The other affected areas were pharyngeal zone,
palatopharyngeal arch and the trachea. In addition during the survey three horses were
diagnosed with occult pulmonary hemorrhage (EIPH).
Regardless of the different upper respiratory tract pathologies in horses, clinical signs are
expressed in a similar way through the presence of unnatural stridor and difficulties in
breathing. In such cases, endoscopy of the respiratory tract provides invaluable help to
accurately locate the injured area and determine the nature of the disorder.
Keywords: horses, upper respiratory disorders, endoscopy
1
Sabev Sasho, PhD, professor, Trakia University of Stara Zagora, Faculty of Veterinary
Medicine, Stara Zagora, Bulgaria.
Corresponding author: Sabev Sasho, email: s_sab@gbg.bg
40

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THE INTERNATIONAL SYMPOSIUM ON ANIMAL SCIENCE

June, 03rd – 08th, 2019. Herceg Novi, Montenegro

21000 Novi Sad, Trg D. Obradovića 8

THE INTERNATIONAL SYMPOSIUM ON

University of Montenegro, Biotechical Faculty - Montenegro

Slovak University of Agriculture in Nitra

National Agricultural and Food Centre

Banat University of Agricultural Sciences and Veterinary Medicine,

European Society of Agricultural Engineers

THE INTERNATIONAL SYMPOSIUM ON ANIMAL SCIENCE (ISAS) 2019

CIP - Каталогизација у публикацији

INTERNATIONAL Symposium on Animal Science (2019 ; Herceg Novi)

Nasl. sa naslovnog ekrana. - Napomene i bibliografske reference uz tekst.

Prof. dr Lidija Perić, Faculty of Agriculture Novi Sad, Serbia, chairman

Prof.dr Nedeljko Tica, Faculty of Agriculture Novi Sad, Serbia

Mr Željka Jurakić, Faculty of Agriculture Novi Sad, Serbia

Provincial Secretariat for Science and Technological Development

Symposium is sponsored by:

3rd to 8th June 2019. Herceg Novi, Montenegro

GENETIC TREND OF MILK YIELD IN STANDARD LACTATION AND

3rd to 8th June 2019. Herceg Novi, Montenegro

DEVELOPMENT TREND IN THE HORSE BREEDING SECTOR OF

Keywords: equidae, horse breeding, development trend, coldbreeds, warmbreeds

3rd to 8th June 2019. Herceg Novi, Montenegro

EXAMINATION OF THE EFFECT OF THE THICKNESS BACON IN THE

Keywords: litter size, thickness of bacon, sows, performance test

3rd to 8th June 2019. Herceg Novi, Montenegro

RELATIONSHIP BETWEEN MILK PRODUCTION AND WHITE CHEESE

Keywords: cows, heat stress, milk yield, white cheese

3rd to 8th June 2019. Herceg Novi, Montenegro

EFFECT OF GENES SHARE HOLSTEIN FRISIAN BREED ON

Keywords: longevity, Holstein Frisian breed, dairy cows

3rd to 8th June 2019. Herceg Novi, Montenegro

ESTIMATION OF GENETIC PARAMETERS FOR MILK TRAITS IN

Keywords: random regression model, milk traits, genetic parameters

3rd to 8th June 2019. Herceg Novi, Montenegro

EFFECT OF THE CRYOPRESERVATION ON THE ELEMENTAL

Keywords: rabbit, endothelial progenitor cells, cryopreservation, macroelements, ICP-OES

3rd to 8th June 2019. Herceg Novi, Montenegro

CHANGES IN THE RELATIVE EXPRESSION OF SELECTED MARKERS

3rd to 8th June 2019. Herceg Novi, Montenegro

THE INFLUENCE OF MOTHER'S AGE ON BIRTH WEIGHT OF LAMBS

Keywords: birth, breed, lamb, sheep, weight.

3rd to 8th June 2019. Herceg Novi, Montenegro

THE IMPACT OF POMEGRANATE ON PRESENCE OF STEROID HORMONE

Keywords: pomegranate, ERα, PGR, ovarian cells, cancer

3rd to 8th June 2019. Herceg Novi, Montenegro

EXTENSITY OF HELMINTOSES OF SHEEP

Abstract: Worldwide, sheep production is affected by nematode parasites, a mjor cause of

3rd to 8th June 2019. Herceg Novi, Montenegro

ANTIMICROBIAL SENSITIVITY PATTERN OF EGYPTIAN AVIAN

Key words: antibiotics, chicken, Egypt, P. multocida, susceptibility

3rd to 8th June 2019. Herceg Novi, Montenegro

FRUIT BYPRODUCTS: A POSSIBLE MODULATORS OF OVARIAN

Keywords: grape, pomegranate, steroid hormones, granulosa cells

3rd to 8th June 2019. Herceg Novi, Montenegro

STAKEHOLDERS’ PERCEPTIONS OF KEEL BONE DAMAGE

3rd to 8th June 2019. Herceg Novi, Montenegro

THE EFFECT OF NUTRITION IN THE FINISHING STAGE ON

Abstract: Quality of bison meat depends on numerous factors, which in terms of

Keywords: bison, feeding, meat quality, oats, pellets - complete feed

3rd to 8th June 2019. Herceg Novi, Montenegro

UTILIZATION OF OLIVE BY-PRODUCTS IN NON-RUMINANT NUTRITION