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S.M.Clearvue - Eng PDF
S.M.Clearvue - Eng PDF
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ClearVue Service Manual (A79210101_04)
ii
Table of Contents
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ClearVue Service Manual (A79210101_04)
iv
Table of Contents
7 - Spares List..........................................................................................................499
8 - Technical Drawings ...........................................................................................505
8-1 - Electrical Drawings.....................................................................................506
8-2 - Pneumatics Drawings .................................................................................507
Appendices..............................................................................................................516
Appendix A - Service Fault Codes .......................................................................517
Appendix B - Parameters.....................................................................................519
Appendix C - Understanding Log Files ...............................................................520
Index...........................................................................................................................535
v
Legal Information
© 2007 Thermo Fisher Scientific. All rights reserved.
Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company
Thermo Fisher Scientific is the trading name of Thermo Shandon Limited
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries
Thermo Fisher Scientific makes every endeavour to ensure that the information
contained in its support documentation is correct and clearly stated but does not accept
responsibility for any errors or omissions. The development of Thermo Fisher products
and services is continuous. Make sure that any published information that you use for
reference is up to date and relates to the status of the product. If necessary, check with
Thermo Fisher or your local Thermo Fisher representative.
This manual may not, in whole or in part, be copied, photocopied, reproduced,
translated, or converted to any electronic or machine readable form without prior written
consent of Thermo Fisher.
All information contained in this manual is proprietary and confidential, and the
exclusive property of Thermo Fisher Scientific. This manual is protected by copyright
and any reproduction is prohibited. This manual is for use only by the individuals to
whom it has been made available by Thermo Fisher Scientific.
Contact addresses
vii
ClearVue Service Manual (A79210101_04)
93/68/EEC.
This IVD equipment complies with the emissions and immunity requirements of IEC
61326-2-6:2006.
This equipment has been designed and tested to CISPR 11 Class A. In a domestic
environment it may cause radio interference, in which case it may be necessary to take
measures to mitigate the interference.
The electromagnetic environment should be evaluated prior to operation of the device.
Do not use this device in close proximity to sources of strong electromagnetic radiation
(eg: unshielded intentional RF sources) as these may interfere with the proper operation.
The Shandon ClearVue™ is referred to throughout this, and other supporting documents, as
the ClearVue.
The Shandon Varistain® Gemini (Inc. ES Variant) is referred throughout this, and other
supporting documents, as the Varistain Gemini.
The Shandon Varistain® 24-4 is referred throughout this, and other supporting documents, as
the Varistain 24-4.
viii
Symbols
The following symbols and conventions are used throughout this manual and on the
instrument.
This symbol is used on the equipment, or in a document, to warn
that instructions must be followed for safe and correct operation. If
this symbol appears on the instrument, always refer to the Operator
Guide.
Note
Notes give more information about a job or instruction but do not form
part of the instructions.
ix
Safety Information
Introduction
Thermo Fisher products are designed for convenient and reliable service; however,
incorrect actions by a user may damage the equipment, or cause a hazard to health.
The following section contains important information for the safe setup and use of the
instrument.
All users must read and understand the following paragraphs before
using the instrument.
General Safety
The instrument, as supplied, conforms with IEC61010-1 and IEC61010-2-101;
however, the addition of chemicals introduces potential hazards.
As with all scientific equipment, due care and good laboratory practice must be employed
when dealing with these chemicals, and consideration must be given to the potential for
hazard when dealing with particular chemicals.
Be aware that many of the reagents used with the Instrument are flammable. Do not
introduce any source of ignition into, or near, the instrument once it has been loaded
with reagents.
Do not remove any panels or covers. The instrument does not have any user serviceable
parts.
The instrument must be properly connected to a good earth (Ground) via the mains
input supply.
Position the instrument such that it is possible to interrupt the Mains supply at the
source by removing the plug from the socket.
If the equipment is used in a manner not specified by Thermo Fisher Scientific, the
protection offered by the equipment may be impaired.
Make sure that there is at least 100 mm (4 in) clearance around any fan inlets on the
instrument.
In compliance with statutory requirements all our equipment is designed to accepted
standards of safety. Its use does not entail any hazard if operated in accordance with the
instructions given in the documentation. However, you must obey the following safety
precautions:
i. All users must read and understand the Operator Guide and this Safety
Information; and only operate the unit in accordance with the instructions.
ii. Potentially lethal voltages above 110V a.c. or 50V d.c. are present inside the
instrument. Do not remove any access covers unless specifically instructed to do so.
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ClearVue Service Manual (A79210101_04)
iii. It is important that normal standards of safety and good laboratory practices are
employed. Always use common sense when operating the instrument.
iv. Any problems and queries should be referred to your Thermo Fisher supplier.
vi. Use only factory approved accessories or replacement parts with this instrument.
xii
Chemical Safety
The introduction of chemicals creates potential hazards and Thermo Fisher has adopted
the following position with regard to the subject of volatile chemicals used in medical
laboratories:
i. Non-specified chemicals are used in the instrument at the customer’s own risk.
ii. All the chemicals recommended by Thermo Fisher have auto-ignition temperatures
considerably above any surface temperature that can be reached during a single
fault failure on the instrument. Small quantities of paraffin wax present will not
reach a temperature that will produce flammable vapour.
iii. The instrument contains no source of ignition in any areas of the instrument where
chemicals are stored or likely to leak in a single fault condition.
iv. The operator is fully aware of the contents of the specification documents detailing
the properties of the chemicals they are using.
v. The operator has carried out any legally required assessment of chemicals used and
is using good laboratory practice.
Environment
This product is required to comply with the European Union’s Waste Electrical and
Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following
symbol:
Thermo Fisher Scientific has contracted with one or more recycling/disposal companies
in each EU Member State, and this product should be disposed of or recycled through
them.
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ClearVue Service Manual (A79210101_04)
Further information on Thermo Fisher’s compliance with these Directives, the recyclers
in your country, and information on Thermo Fisher products which may assist the
detection of substances subject to the RoHS Directive are available at:
www.thermo.com/WEEERoHS.
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A79210100_06
1 - Introduction to the ClearVue
1.1 Design Purpose
1.2 Compatibility
1.3 Identification of parts (front)
1.4 Inside the ClearVue
1.5 Identification of parts (rear)
1.6 Example of a Varistain Gemini basket
1.7 System interfacing
1.8 System specification
1.9 Coverslipping method
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Varistain 24-4
Leica Autostainer
The ClearVue is capable of having multiple baskets loaded at once in any order from any
of the above stainers.
High quality, 75 x 25mm microscope slides must be used with the baskets.
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1.2 Compatibility
The ClearVue is compatible with the following sizes of coverslips:
No. 1.5 x 24 x 40mm
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Stopping and restarting the ClearVue is controlled by the software to ensure safety, whilst
at the same time making sure that the samples are not compromised.
Opening any of the doors will not necessarily cause the ClearVue to
stop running; therefore care should be taken when adding or removing
baskets.
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This method of coverslipping has been proven, through rigorous testing, to minimize
bubbles and ensure complete adhesion to the slide.
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2 - Basic Operation
2.1 Daily tasks
2.2 Weekly tasks
2.3 Level the ClearVue before use
2.4 Change the carbon filter
2.5 Fit the extraction kit
2.6 Maintaining the Xylene tray level
2.7 Fill the mountant bottle
2.8 Change the purge tray and debris tray
2.9 Start up the ClearVue
2.10 Fill the dispense head cleaning station
2.11 De-gas the mountant bottle
2.12 Flushing the system
2.13 Purging the system
2.14 Remove and replace the coverslip hopper
2.15 Shutting down the ClearVue
2.16 Load a basket
2.17 Unload a basket
2.18 Abort a basket
2.19 Manually abort a basket
2.20 Remove baskets from the Load & Unload rail
2.21 Remove slides during coverslipping
2.22 Remove baskets from the vertical lift
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Top-up the Dispense head cleaning station . Wipe the top with a xylene
damp cloth
Wipe the Suction cup with a xylene damp cloth to ensure it is clean and free
from debris. Replace if necessary . Ensure the Suction cup is dry before use
Purge the system 3 times . Lift the coverslip hopper before purging
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Remove any discarded coverslips from the Slip Dispense area and clean the
Slip dispense carriage
Remove the Coverslip transfer head to check that the Pads and the Suction
cup are clean and free of Mountant. Wipe the Pads with a xylene damp
cloth to clean
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Levelling is carried out on the jackable feet of the ClearVue to raise or lower any of the
four corners of the instrument.
The following procedure should be carried out on all required corners of the ClearVue:
1. Use a 13mm wrench (spanner) to loosen the Lock Nut of the Jackable foot.
2. Adjust the Jacking Nut with the wrench to raise or lower the ClearVue as required.
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2. Lift the Carbon filter out of the chamber and dispose of according to local
regulations.
3. Remove the cellophane wrapping from a new Carbon filter and place into the
chamber.
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5. Slide the Filter cover back into position making sure it is firmly clipped into
place.
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Jubilee Clip
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2. Carefully pour xylene into the Xylene Tray or remove using a pipette as required.
3. The depth of the xylene should be approximately half-way up the Anti-Surge Baffle.
4. Slowly close the Xylene Tray completely to ensure that the seal is properly engaged.
Alternatively, it is possible to open the Load door and pour xylene into the Xylene Tray
using a small beaker. If this method is used and there are no Baskets waiting on the Load
Rail, the ClearVue will perform a routine check after the Load Door has been closed.
If Baskets are loaded the ClearVue will begin coverslipping when the Load Door is
closed.
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The Mountant Bottle supplies Mountant to the Dispense head. It is strongly advised not
to allow the Mountant bottle to run dry.
To fill the Mountant bottle:
1. Remove the Mountant bottle cap.
2. Carefully pour Mountant into the open end of the Mountant Bottle until the liquid
reaches the bottom of the collar. Ensure no Mountant is spilt down the outside of the
bottle.
3. Replace the Mountant bottle cap and twist clockwise by hand to tighten it.
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Notes:
After filling, de-gas the mountant bottle.
If the Mountant Bottle has been allowed to run dry, or the level has fallen below the bottom of
the internal pipe, it will be necessary to:
1 - Fill the Mountant Bottle.
2 - De-gas the Mountant Bottle
3 - Flush the system to remove any air from the pipes
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2. Remove the Debris Tray and dispose of the contents according to local regulations.
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3. Reach into the instrument as shown and, using the tab, carefully lift the Purge Tray
down off its shelf.
5. Either:
Unpack a new Coverslip Hopper; the end cap doubles as a new Purge Tray, or
Use one of the spare Purge Trays supplied with the instrument.
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4. When the Language select screen appears, use the arrow keys on the Touch screen to
highlight the required language, and then press Select.
5. The Main menu now displays. The system will carry out a series of automated checks
and the Status will show Initialising. When complete the Status will show OK. The
instrument is now ready to use.
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The Dispense head cleaning station ensures the Dispense head does not get blocked with
Mountant. There should be a visible pool of Xylene in the central well.
To fill the Dispense head cleaning station:
1. From the Main menu, press the Mountant key on the screen.
4. Wait for activity within the ClearVue to stop, and Access Ready to flash on the
screen.
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6. The Dispense head cleaning station is now be accessible from above. It can either
be removed, by pulling slightly forwards and then lifting it gently from its fitting, or
left in the ClearVue.
7. Drip Xylene into the Cleaning station until it is at a level where it can be seen in
the central well (approx. 18ml).
8. Use the end of the pipette to push the central well down, to ensure that the
Xylene levels are consistent.
Note:
Ensure that the central well re-seats itself properly.
9. If the Dispense head cleaning station has been removed from the instrument, it
can be filled easier by removing the Dispense head cleaning station lid and pouring
xylene in up to the required level.
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10. When finished, close the Access door and press the Complete key.
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5. Ensure the Purge tray is empty and that there is sufficient Mountant (or Xylene)
in the Mountant bottle (at least 80ml).
6. Press the Start key.
7. Wait for the activity within the ClearVue to stop, and then empty or discard the
Purge tray.
8. Press the Exit key.
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5. Ensure there is sufficient room in the Purge tray before starting the Purge cycle.
6. Press the Start key.
7. Wait for this screen to appear and the activity within the ClearVue to stop and
then empty the Purge tray.
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2. Remove the empty Coverslip hopper by pulling slightly forwards and then lifting it
gently upwards.
Note:
If the Coverslip Hopper is not empty, use the end of the paintbrush provided to push the foam
inside the Hopper down until it secures the remaining coverslips.
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3. Unpack a new Coverslip hopper and identify the Dispense directional arrow on the
top.
Note:
Ensure coverslips are the correct size. Ensure Coverslip Hopper has not been dropped by checking
for broken coverslips.
4. Remove the end cap from the bottom of the Coverslip Hopper and retain for use as a
Purge Tray.
5. Load the new Coverslip Hopper ensuring that the Dispense Directional Arrow is
pointing towards the back of the instrument.
6. Ensure the Coverslip retention spring is located inside the slot in the Hopper
mounting block.
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7. The Coverslip hopper should sit level and fully down over the location pins.
Note:
Clear Count must always be selected after changing the Coverslip size.
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2. Insert Slides, starting from the bottom shelf, with the sample on the top.
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4. When all the required Slides are in the Basket, return the Slide Retainer to it’s original
position to secure the Slides in the Basket.
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5. The Basket is now ready to load onto a stainer or directly into the ClearVue.
2. Gently shake the Basket to remove excess xylene and blot on absorbant paper.
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Notes:
Ensure Slide Retainer is closed before loading basket.
Ensure Directional Arrow on basket is pointing upwards.
If operating outside of the recommended temperature range, ensure samples do not dry out too
quickly by loading no more than 2 Baskets at any one time.
4. Close the Load Door.
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2. Wait about 15 mins before removing a completed Basket, to allow the Mountant to
dry enough to allow the Slides to be handled safely.
Keep the Basket upright after unloading - Basket Rack is supplied for this purpose.
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Any Baskets which are unloaded manually may contain slides which
have not been coverslipped, and steps should be taken to preserve the
specimens.
If any Baskets are removed manually due to a power failure or
blockage, the ClearVue must be switched Off and On again to allow
the mechanism to re-calibrate.
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2. Remove any Baskets which are located directly underneath the Load Door.
Note:
If there are Baskets further along the Load Rail they must be brought back underneath the Load
Door before they can be removed.
3. To move Baskets back underneath the Load Door insert the Screwdriver (supplied)
into the hole at the side of the instrument, under the Load Door.
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4. Turn the Screwdriver clockwise to bring the Basket Pusher as far over to the left-
hand side of the instrument as possible.
5. It should then be possible to reach through the Load Door and pull any remaining
Baskets over to where they can be removed.
Note:
There is a ball-spring plunger half-way along the Load Rail which the basket must be pulled
over.
2. Remove any Baskets which are located directly underneath the Unload Door as
normal.
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3. If the Dispense Head is over the Slide, reach through the Access Door and manually
pull it as far towards the front of the instrument as possible.
4. Rotate the Coverslip Transfer Head until it is in its top position to provide access to
the Slide.
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Notes:
The right-hand Gripper jaw is spring loaded and can be moved to the right to release the slide.
It is advisable to remove the Debris Tray and then reach into the instrument through the Debris
Tray Door in order to support the Slide when removing it from the Gripper jaws.
6. Reach in through the Load Door and pull the long Gripper jaw as far towards the left
of the instrument as possible.
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2. If there are any Baskets halfway onto the Lift Block, it may be necessary to lower the
Lift Block to remove them.
3. Reach in through the Unload Door and push the Basket Unload Bracket as far
towards the right of the instrument as possible.
4. Reach in through the Load Door and pull the long Gripper jaw as far towards the left
as possible.
5. Insert the Screwdriver into the small hole in the Main cover.
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7. Turn the Screwdriver clockwise to raise the Basket until it is level with the Unload
Rail.
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Note:
Do not turn the Screwdriver anti-clockwise once the Slides are above the Slide Guide as this
could result in broken Slides.
8. Close the Slide Retainer as soon as possible to ensure that no Slides fall out of the
Basket.
9. Once the Basket is aligned with the Unload Rail, pull the Basket onto the Unload
Rail and remove as normal.
If the Gripper jaws and Basket Unload Bracket are not correctly
located as described above, any attempt to raise the Basket may lead
to damage to the instrument, Basket or specimens.
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3 - Settings
3.1 Adjust the coverslip position
3.2 Select the coverslip position
3.3 Change the coverslip transfer head
3.4 Adjust the screen settings
3.5 Change the time and date setting
3.6 Change the system language
3.7 Create the engineers' log
3.8 Update the system software
3.9 Adjust the mountant dispense volume
3.10 Slide retainer types
3.11 Install the offset coverslip transfer head
3.12 Alter the slide sensing parameter
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3. Use the Arrow Keys on the Slip Adjustment screen to move the Coverslip to the
required position. The default position has a setting of 0.
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Examples
The positive number values indicate a movement The negative number values indicate a movement
in the direction of the labelled arrows; hence the in the opposite direction to the labelled arrows;
Coverslip is positioned above and to the right hence the Coverslip is positioned below and to
hand side of centre. the left hand side of centre.
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The 40mm Coverslips can be positioned centrally, and also offset by 5mm when using
the Offset coverslip transfer head.
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4. Move the Coverslip transfer head into the position specified (see Section 3.3).
5. Press OK on the Position Setup screen.
Note:
When using the Offset coverslip transfer head with 40mm Coverslips, the ClearVue
automatically places the Coverslip on the slide in the correct position, regardless of whether the
End or Centre position has been selected.
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4. Use the arrow keys to adjust the View Angle and Brightness.
5. When the Touch screen is configured, press OK to return to the Options menu.
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4. Adjust the Time and the Date using the relevant arrow keys.
5. Adjust the Time Format: use the 24 hour key to set the time to the 24-hour format.
The 12 hour key then displays to let you change the format to 12-hour.
6. Adjust the Date Format: use the mm/dd key to set the date to that format. The
dd/mm key then displays to let you change the format to dd/mm.
7. When the settings are configured as required, press Set to apply the settings.
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3. The Language Select screen displays. Use the arrow keys to highlight the required
language and press Select.
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5. The ClearVue displays the Wait screen and writes to the floppy disc.
6. When the Wait screen has gone and the light on the Disc drive has gone out, it is safe
to remove the floppy disc from the Disc drive.
Note:
An Engineers’ log can only be written when the ClearVue is idle (not processing baskets). An
audible warning sounds if you attempt to write an Engineers’ log during processing.
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The Disc Options screen allows the ClearVue software to be updated. A Software
Update would normally be performed by a Service Engineer; however, it is possible for
the user to perform an update if supplied with a new version on a floppy disc.
To perform a Software update:
1. Insert the floppy disc, containing the updated version of the software, into the Disc
drive. From the Main menu press the Options key on the Touch screen.
4. The ClearVue will then automatically install the updated software and reboot itself.
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3. Use the Arrow keys on the Mountant Volume screen to adjust the dispensed volume
for each Basket type. The recommended setting for general use is 13.
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The Baskets supplied with the ClearVue have a Black slide retainer. Baskets with White
slide retainers are available as an Accessory (see Appendix A1). The Black slide retainer
typically designates thicker samples; whilst the White slide retainer designates thinner
samples.
The ClearVue can detect the colour of the Slide retainer and automatically adjust the
amount of Mountant dispensed, based on the user defined settings, to ensure that the
Coverslip adheres properly to the slide.
Note:
If there is very little variation in the thicknesses of samples, the volume of Mountant dispensed
for Baskets with either a black or a white Slide Retainer can be set to the same value.
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2. Detach the Pneumatic pipe connecting the current coverslip transfer head to the
Shandon ClearVue: hold down the blue portion of the Pneumatic connector on the
Rotatable Fitting and pull out the Pneumatic pipe.
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A. Fit the Offset head B. Align the Reference mark C. Hold the Offset head and
onto the Location pins. with the Default position. push one end of the pneumatic
Tighten the Thumbscrew by pipe firmly into the pneumatic
turning it clockwise. connector. Do not push down
on the connector.
D. Insert the other end of the
pipe into the connector of the
Rotatable fitting on the
ClearVue.
5. Pull firmly on the Pneumatic pipe to make sure it is correctly fitted into the
Pneumatic collars.
Note:
When the Offset coverslip transfer head is fitted, the ClearVue automatically places the 40mm
coverslip on the slide in the correct position.
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4. Press the shaded area of the slide for approximately 3 seconds to display the Slide
sensing adjustment screen.
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5. Initially, the default value (95) is displayed on the left side of the screen. Use the
bottom arrow to reduce the value in steps of 5. If ClearVue is missing slides when
processing, initially reduce the value by 10.
6. Press OK to exit the screen and return to the Options menu.
If the problem persists, further reduce the Slide sensing value by 5 at a time. The
lowest possible value is 40.
Note:
Adjusting the slide sensing will not affect the quality of coverslipping but it might reduce the
speed.
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4 - Maintenance
The following sections give details on how to carry out basic maintenance procedures.
If a problem occurs which is not covered in these sections contact the Thermo Fisher
Scientific Service Department.
4.1 Cleaning and maintenance cautions
4.2 Replacing seals
4.3 Removing the mountant bottle
4.4 Preparing the ClearVue for storage
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3. Identify the black O-Ring seal at the base of the Mountant Bottle spout (shown here
in yellow).
4. Use a pair of scissors or wire-cutters to carefully cut the O-Ring seal, ensuring none of
the surfaces of the Mountant Bottle are damaged in the process.
5. Replace with a new seal:
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Note:
The diagrams show the Mountant bottle spout seal replacement, but the technique is common to
the Xylene tray seal also.
A. Carefully stretch the O-Ring B. Once it is over the C. Allow the O-Ring seal to snap
seal to fit over the Retaining Retaining surface, roll into place, then ensure it is
surface. it towards the Recess. properly seated in the Recess.
1. Ensure that there are no Baskets currently being processed within the ClearVue.
2. Taking care not to spill any remaining xylene, remove the Xylene Tray and empty out
any xylene.
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4. Use a pair of scissors or wire-cutters to carefully cut the O-Ring seal, ensuring none of
the surfaces of the Xylene Tray are damaged in the process.
5. Replace with a new seal.
Refer to Sections 5.2 and 5.3 for information about cleaning the Mountant bottle spout
seal and the Xylene tray seal respectively.
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After Flushing the system with Xylene during the initial set-up of the ClearVue, the
Mountant Bottle needs to be emptied of any remaining Xylene.
To remove the mountant bottle:
1. Remove the mountant bottle cap by twisting it anti-clockwise to loosen it.
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3. Insert the Allen key (as provided) into the Mountant Bottle fitting connector. Turn
the Allen key anti-clockwise to loosen the bottle and lift the bottle out of the
ClearVue.
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5 - Cleaning
The following sections give details on how to carry out basic cleaning procedures.
If a problem occurs which is not covered in these sections contact the Thermo Fisher
Scientific Service Department.
5.1 Mountant bottle cap
5.2 Mountant bottle seal
5.3 Xylene tray seal
5.4 Dispense head cleaning station
5.5 Suction cup
5.6 Slip dispense carriage
5.7 Mountant dispense needle
5.8 Touch screen
5.9 Slip dispense skirt
5.10 Gripper return plate
5.11 Slide grippers
5.12 Coverslip transfer head
5.13 Camera
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If the Mountant bottle cap becomes encrusted with Mountant and difficult to open, the
following procedures should be followed to remove and clean the bottle cap:
To remove the mountant bottle cap:
Once the Mountant bottle cap has been removed, the following cleaning procedure
should be followed:
1. Use a xylene soaked cloth to remove any dried Mountant from the bottle spout. Pay
particular attention to the threaded portion and its 3 cut-outs.
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Hand tighten the Mountant bottle cap only - do not use the cap
removal tool!
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3. Identify the black O-Ring seal at the base of the Mountant bottle spout (shown here
in yellow).
4. Carefully prise the O-Ring seal from its seat, ensuring none of the surfaces of the
Mountant bottle are damaged in the process.
5. Manoeuvre the O-Ring seal over the large diameter threaded portion of the Mountant
bottle spout.
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A. Carefully stretch the O-Ring B. Once it is over the C. Allow the O-Ring seal to snap
seal to fit over the Retaining Retaining surface, roll into place, then ensure it is
surface. it towards the Recess. properly seated in the Recess.
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2. Taking care not to spill any remaining xylene, remove the Xylene tray and empty out
any xylene.
4. Carefully prise the O-Ring seal from its seat, ensuring none of the surfaces of the
Xylene tray are damaged in the process.
5. Manoeuvre the O-Ring seal onto the larger adjacent portion of the Xylene tray.
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6. Roll the O-Ring seal along the surface of this portion until it comes off.
7. Carefully guide the O-Ring seal over the rest of the Xylene tray until it is clear.
2. Check the seal for signs of tearing or splitting, and replace if necessary.
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4. Wait for activity within the ClearVue to stop, and Access Ready to flash.
6. The cleaning station is now accessible: carefully pull it upwards to remove from its
fixing.
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7. Remove the lid and dispose of the contents according to local regulations.
8. Use a xylene damp cloth to clean all the surfaces of the cleaning station including the
top of the internal parts.
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10. Reassemble the cleaning station and refit into the ClearVue.
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A. Drip Xylene into the dispense head cleaning station until it is at a level where it
can be seen in the central well (approx. 18ml).
B. Use the end of the pipette to push the central well down, to ensure that the xylene
levels are consistent.
12. When finished, close the Access door and press the Complete key on the screen.
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3. Remove the Suction cup by pushing down from the top of the rubber.
3. Remove the suction cup from the xylene and allow to air-dry. Make sure the suction
cup is fully dry before refitting.
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2. Ensure the suction cup protrudes from the base of the coverslip transfer head.
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B. Replace the Coverslip transfer head onto the Location pins in either the Default
or Central position. Tighten the Thumbscrew by turning it clockwise.
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C. The Reference mark on the top of the Coverslip transfer head indicates which
position it is in: either the Default or End position.
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2. Remove the Coverslip hopper by pulling it towards the front of the ClearVue and
then lifting it gently upwards.
3. Without turning the Coverslip hopper upside down, check the bottom of the Hopper
for any broken Coverslips.
Broken glass may be present!
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4. Using the Brush provided, clear the top of the Slip dispense carriage of any glass
debris.
5. Make sure the Groove and vacuum ports are clear of glass dust. If the Vacuum ports
are blocked use an implement (less than 1mm (0.039”) dia.) to push the blockage
through.
6. Remove the Slip dispense skirt and clean in the recess (see Section 5.9).
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7. Move the Slip Dispense Carriage forwards if necessary by pressing the Access button
on the Mountant Screen and then following the steps given in Section 2-10 for access
to the Dispense Head Cleaning Station.
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2. Unscrew the Mountant dispense needle assembly and lift it out of the Support arm.
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After cleaning:
Flush the system
Ensure the Dispense head cleaning station is topped up
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3. The Cleaning screen displays, in which it is possible to touch the screen without any
effect. A countdown timer shows how long the cleaning screen will continue to be
displayed for.
4. To clean the touch screen, wipe with a soft, water-damp cloth. Thoroughly dry using
a lint-free cloth.
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Pay particular attention to the areas where the plate contacts any
other surfaces.
Ensure that the Gripper return plate can move freely from side to
side.
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If the Gripper return plate is heavily contaminated with Mountant, remove it for a more
thorough clean:
1. Locate the Gripper return plate.
2. Using the protruding handle, pull the Gripper return plate to the right and then up.
It should now lift off its Location pins:
3. Soak the Gripper return plate in xylene to remove any Mountant. Use xylene to clean
the Location pins and other contact areas.
4. Replace the Gripper return plate:
Ensure that the Gripper return plate can move freely from side to
side.
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3. Identify the Slide grippers. To improve access to the Slide grippers, push the Basket
unload bracket as far over to the left as it will go.
6. If the debris is stuck with dried Mountant, use a pipette to apply xylene to soften the
Mountant to allow removal.
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7. Use a xylene-damp cloth to wipe any dried Mountant from the Slide grippers. Pay
particular attention to both of the surfaces of the ’V’ on both of the Gripper jaws.
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If the internal airway in the Coverslip transfer head becomes blocked it can be cleaned as
follows:
1. Remove the Coverslip transfer head:
2. Detach the Pneumatic pipe from the top of the Coverslip transfer head: hold down
the blue portion of the Pneumatic connector and pull out the Pneumatic pipe.
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3. Ensure that the internal airway is free from blockages such as glass debris or dried
Mountant. The Coverslip transfer head can be soaked in xylene to aid removal of
dried Mountant.
4. Once clean, remove the Coverslip transfer head from the xylene and allow to air-dry.
5. Refit the Coverslip transfer head making sure that the Pneumatic pipe is fully inserted
into the Pneumatic connector:
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B. Replace the Coverslip transfer head onto the Location pins in either the Default
or Central position. Tighten the Thumbscrew by turning it clockwise.
C. The Reference mark on the top of the Coverslip transfer head indicates which
position it is in: either the Default or End position.
Note:
Pull firmly on the Pneumatic pipe to check that it is correctly fitted into the Pneumatic
connectors.
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6 - Troubleshooting
6.1 Error screens
6.2 Perform a bead test
6.3 Identifying problems with slides
6.4 Troubleshooting tables
6.4.1 Machine dead - no power
6.4.2 Dispense needle dirty/blocked
6.4.3 Slide jams
6.4.4 Coverslip misdispense
6.4.5 Coverslip misdispense (cont'd)
6.4.6 Breaking coverslips
6.4.7 Basket jams
6.4.8 Breaking slides
6.4.9 Mountant overspill
6.4.10 Skewed coverslips
6.4.11 Slides not coverslipped
6.4.12 Part broken coverslips
6.4.13 Slides too wet
6.5 Diagnosis flowchart
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Pressing the flashing Error message will display an Error Screen which provides one or
more of the following options to rectify the error.
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4. Place a Basket of dry slides on the Load rail. Do not close the Load door.
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6. The image of the coverslip will disappear and the ClearVue will emit an ascending
tone.
8. When processing is complete, remove the Basket and lay the slides out on a flat
surface.
Note:
The Mountant will run when it is still a liquid, so ensure the slides are kept flat at all times.
Check the quality of the prepared slide (see Section 6.3)
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Larger drop at end, close to, but not encroaching on the frosted area
The Diagnosis flowchart is intended to aid in the diagnosis of problems relating to the
quality of the Mountant dispense (see Section 6.5).
Correct appearance of coverslipped slide:
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Note:
There may be a slight variation in the position of the Coverslip depending on the quality of the
Coverslips used.
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Multiple bubbles visible at non- Suction cup Remove Suction cup and
frosted end of slide contaminated or clean using a xylene-damp
broken cloth.
Note: Dry suction cup fully
before refitting.
Replace Suction cup
Ensure pipe to Coverslip
transfer head is properly
inserted
Straight line of bubbles down the Suction cup Remove Suction cup and
length of slide contaminated with clean using a xylene-damp
Mountant cloth
Note: Dry suction cup fully
before refitting
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replace if necessary.
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Single bubble at Non-frosted end Dispense Head too Check Dispense Head is
of slide high fully screwed down
Contact Thermo Fisher
Scientific Service
department
Often located underneath the Suction cup Remove and clean Suction
Suction cup contaminated with cup using a xylene-damp
Mountant cloth
Note: Dry suction cup fully
before refitting
Single bubble at Frosted end of Dispense Head too Check Dispense Head is
slide high fully screwed down
Contact Thermo Fisher
Scientific Service
department
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Department
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rail
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Distinct area with no mountant Debris under the Ensure coverslips are
coverslip
free from debris and
dust
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Slide jams
Coverslip misdispense
Breaking coverslips
Basket jams
Breaking slides
Slides are too wet when removed from the Unload rail
Skewed coverslips
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The ClearVue does not The ClearVue is still Wait approximately 30 seconds for
respond when the mains starting-up. the start-up sequence to finish.
power is switched on.
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Mountant dispense Low xylene level in Dispense Refill the Dispense head
needle dirty/blocked. head cleaning station. cleaning station fully
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Slide Basket Slide Retainer is not Ensure the Slide Retainer is fully closed
jams. fully closed. prior to loading the basket.
Skewed slide loaded into Ensure slides are loaded correctly into the
basket. basket.
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Coverslip Suction Cup not fitted Ensure Suction Cup is pushed fully up
misdispense correctly. against the metal collar and does not
(cont'd) protrude more than 1mm.
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Basket Slide Retainer not Ensure Slide Retainer is fully closed prior
fully closed. to loading basket.
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Basket Slide Retainer not fully Ensure Slide Retainer is fully closed
closed. prior to loading basket.
Slide loaded into basket Ensure slides are correctly loaded into
incorrectly. baskets.
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for support.
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Too much Too much xylene carry- Shake Basket to remove excess
Mountant overspill over from staining. xylene before loading.
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Skewed Baskets not stored level when Ensure Drying Rack is on a level
coverslips Slides are wet. surface.
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Slides not Gripper return plate is stuck with Clean Gripper Return Plate.
coverslipped mountant and prevents the gripper
jaws closing fully on the slide (User
Error 28).
ClearVue is not seeing the slides (No Adjust the Slide Sensing
User Error). Parameter.
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Slides are too wet Insufficient drying time Leave Baskets on the Unload Rail
when removed allowed. for a minimum of 15 minutes.
from the Unload
rail.
Too much xylene carry- Shake Basket to remove excess
over from staining. xylene before loading.
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Appendix A1 - Accessories
Item Part Number
Coverslip Hopper, Pk. of 2 (24 x 40mm, #1.5, 500 off) A79210050
Coverslip Hopper, Pk. of 2 (24 x 50mm, #1.5, 500 off) A79210051
Coverslip Hopper, Pk. of 2 (24 x 55mm, #1.5, 500 off) A79210052
Coverslip Hopper, Pk. of 20 (24 x 40mm, #1.5, 500 off) A79210074
Coverslip Hopper, Pk. of 20 (24 x 50mm, #1.5, 500 off) A79210075
Coverslip Hopper, Pk. of 20 (24 x 55mm, #1.5, 500 off) A79210076
Gemini Basket with Black Slide Retainer (Pk of 5) A79210064
Gemini Basket with White Slide Retainer (Pk of 5) A79210065
V24 Basket with Black Slide Retainer (Pk of 5) A79210069
V24 Basket with White Slide Retainer (Pk of 5) A79210070
Sakura Basket with Black Slide Retainer (Pk of 5) A79210066
Sakura Basket with White Slide Retainer (Pk of 5) A79210067
Leica Basket with Black Slide Retainer (Pk of 5) A79210072
Leica Basket with White Slide Retainer (Pk of 5) A79210073
Vent Extraction Kit A79210080
V24 Kit A79210071
Sakura Hanger Kit (Pk of 5) A79210068
Cytology Loading Tray A79210092
Offset Coverslip Transfer Head Kit A79210099
Shandon ClearVue Mount XYL 4212
Shandon ClearVue Mount 4211
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Toluene
Ethanol
Isopropanol (IPA)
Water
Mountants
Shandon ClearVue Mount XYL
Note:
All Mountants are available from Thermo Fisher Scientific.
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3. Dry
3. Dry
2. Dry
Remove the Dispense head and place in a suitable container to allow it to drain
Remove the Mountant Bottle and rinse with xylene, or replace with a fresh bottle
Pour 25ml of xylene into the clean Mountant Bottle and Flush the system
When the Flush cycle is complete, check that the Mountant Bottle Cap is clear of
Mountant
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Place the Dispense Head Cap over the Dispense Head Needle to prevent the
xylene which remains in the system from leaking out during transit
Ensure the Battery Switch has been set to the off ’O’ position
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1 - Technical Description
1-1 Introduction and Safety
1-2 General Description
1-3 Basic Structure
1-4 Electronics Assembly
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Safety
The Safety and Warranty Booklet (A79910001) and the ClearVue Operator Guide
(A79210100) should both have been read and understood before using this instrument.
In addition, the following statements must be read and understood before commencing
any work on the ClearVue.
i. Potentially lethal voltages above 110Vac are present inside this instrument.
Do not remove any access covers unless trained to do so. Disconnect the
instrument from the mains power and switch the Battery Isolator Switch to
the off ’O’ position before carrying out any work on an area enclosed within
an access panel.
ii. The ClearVue must be properly connected to a good earth (ground) via the
mains input supply. All earth (ground) straps and connections must be
replaced correctly after service and before switching the instrument on.
iii. Wear earthing (grounding) wrist straps and earth (ground) yourself before
handling connectors or electronic components, as static electricity can
damage sensitive electronic parts. Damage from static electricity can take
weeks or months to develop.
iv. The ClearVue is heavy and requires at least 2 people to lift it.
v. As supplied, the ClearVue conforms to IEC61010-1 and IEC61010-2-101;
however the addition of chemicals introduces potential hazards. Follow good
laboratory practice when dealing with these chemicals, and be aware that
chemicals used with the ClearVue may be flammable. Do not introduce
sources of ignition into, or near, the instrument once it has been loaded with
chemicals.
vi. Refer to laboratory procedures and manufacturer’s data sheets when using
reagents. Only use reagents specified in Appendix B of the ClearVue
Operator Guide.
vii. Ensure you employ normal standards of safety and good laboratory practice.
Think about your actions - always use common sense and the best known
practice when operating the instrument.
viii. If the ClearVue has been used with materials which are toxic or
contaminated with pathogenic micro-organisms; follow the cleaning
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Special slide baskets compatible with a variety of stainers from Thermo Scientific
and from other manufacturers.
Compatibility with a range of coverslip sizes and positions on the slide.
Up to 5 baskets of slides can be loaded at any one time, with the capability of
adding further baskets without interrupting coverslipping.
A downdraft system removes unpleasant fumes away from the operator’s face
when the unload door is opened.
Windows in the doors and internal illumination allow the operator to see the
coverslipping in progress.
The instrument uses a touch screen with clear instructions and information.
There is battery back-up for enhanced specimen security in the event of a power
failure.
Baskets are available with black and white sliders which can be used to select
different mountant volumes for different specimen types.
Pre-loaded coverslip hoppers for reduced glass handling.
Internal camera takes images of the baskets and slides for accurate determination
of basket type, slider colour and slide positions.
Full error recovery instructions.
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The input rail acts as a buffer for the 5 baskets that can be loaded on to the ClearVue at
once. The input rail will then push each of the baskets in turn onto the lift block. The
ClearVue counts the numbers of baskets on the load rail by measuring the position of the
first latch in relation to the pop out pin as it stalls against the baskets. A xylene trough sits
below the input rail to prevent drying of slides while the baskets are waiting to be
processed.
The basket is pushed onto the lift block and retained against 2 pins by a ball spring
pushing on the basket flag; this is essential as it holds the basket in a controlled position
for slide processing. The vertical lift carries the basket up through the ClearVue so that
the slides can be processed. At the end of processing the vertical lift then carries the
basket past the unload rail so that a cam follower can push the slider close block forwards
to partially close the basket slider; this retains the slides during unloading. After this the
basket is moved level with the unload rail ready for the basket Eject to push it onto the
unload rail.
The basket open assembly opens the basket slider (basket slide retainer) after the basket is
pushed onto the lift block. After the basket is opened the slide retainer and slider pusher
are used to retain the slide in the basket as it is moved upwards. The slider pusher is
spring loaded both to ensure the basket is opened correctly and to help fully open baskets
that are loaded when partially open.
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The slide guides main function is to guide each slide into a position where it can be both
removed from and replaced into the basket without touching the basket. To do this the
spring loaded slide guide assembly locates on the end of the basket and guides each slide
using 2 small guide surfaces and a leaf spring. The 2nd function of the slide guide is to
retain the slides in the basket after they pass above the basket open assembly and until
they are removed by the grippers.
The slide grippers remove the slides from the basket and move them into the
coverslipping area. The V in each gripper jaw lifts the slide to a fixed and consistent
position for a good coverslipped slide. The gripper return plate fitted to the short gripper
jaw is used to push the slide away from the jaw even if the slide is tacky with drying
mounting media. A sensor is used to detect the position of the short jaw which gives the
ClearVue the ability to both measure and automatically adjust for different slide widths.
This same sensor also enables the ClearVue to detect if a slide has jammed when being
placed back into the basket.
The vacuum assisted coverslip dispense takes a coverslip from the bottom of the hopper
and presents it ready to be picked up by the coverslip transfer assembly. The coverslip
dispense includes two reflective optical sensors that are used to detect the size of coverslip
as well as checking the quality of the dispensed coverslip. The coverslip dispense can
detect double dispenses, missing coverslips, broken coverslips, skewed coverslips, chipped
coverslips, and coverslips that are too long or short. A skirt is used on the coverslip
dispense to sweep rejected coverslips and debris into the debris tray.
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The mountant dispense head dispenses a bead of mountant onto the slide with a precisely
controlled shape. The shape of this bead is designed to flow in a certain way and allows a
coverslip to be placed on the slide without trapping any air bubbles.
The coverslip transfer head picks a coverslip up off the coverslip dispense carriage and
places it onto the slide.
When the coverslip is placed correctly onto the mountant bead on a properly cleared
slide the mountant flow should be even on either side of the coverslip. The flow should
move quickly down 2/3 of the slide and then should continue slowly to the end with a
small pause before the transfer head moves away.
The shape of the transfer head, the position of the sprung pads and the suction cup are all
designed to hold the coverslip in a specific shape and position. This coverslip shape,
positional control of the coverslip transfer motor, bead shape and vacuum decay time are
all critical to ensuring that the mountant flow is correct and that coverslip is placed
without trapping any air.
The transfer head can be positioned to suit coverslips located at the end of the slide or 40
coverslips placed centrally on the slide. An optional Cytyc specific head is also available as
an accessory.
The upper slide retention is mounted on the basket eject bracket and has 2 stages. The
first stage is fixed and just ensures that the slide has not moved too far out of the basket
during index from the coverslipping position. The second stage is spring loaded and
pushes the slides against the basket so that the basket slider can be closed without
breaking any slides. After the basket has been closed by the vertical lift and returned to
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the unload rail the eject bracket pushes it off the lift block and far enough along the
unload rail so that it can be unloaded by the operator.
The output rail acts as a buffer for baskets after they have been processed on the
ClearVue, leaving the basket for 15 minutes on the output rail after processing has
finished will give much more stable coverslips that are less prone to moving.
The mountant pump dispenses a precise amount of mountant onto the slide to form the
bead. The movement of the pump is synchronised with the dispense head to ensure the
correct bead shape and mountant volume. The syringe holds 2.5ml of mountant in its
fully charged position.
The cleaning station dips the dispense head needle into solvent to clean it between
baskets and to prevent dried mountant blocking the needle when the ClearVue is not in
use.
The mountant bottle is simply a reservoir for storing the mounting media. The ClearVue
has the facility to pressurize the bottle during the syringe load cycle. There is also a de-gas
option which can be used to remove air trapped in the mountant.
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The pneumatic circuit provides both positive pressure and a vacuum. There are two
pumps in the circuit, both of which are capable of producing either vacuum or pressure.
The first pump connects via the upper solenoid bank to the bottle and provides either
pressure for loading the syringe or vacuum for the de-gas function. The second pump is
used for vacuum only and connects via the lower solenoid bank to either the coverslip
dispense carriage or the coverslip transfer head. An extremely sensitive exhaust is
connected to the transfer head solenoid; controlling the exhaust (decay) time is critical to
getting the correct mountant flow between the coverslip and the slide (see Section 1-2-8).
The covers on the ClearVue are ergonomically designed from a tough polymer material
to act both as safety covers and as a flameproof enclosure. The windows are made from
an aerospace grade material which is the clearest available that is both flameproof and
resistant to ClearVue solvents. The filter used is a standard carbon filter.
1-3-16 - Camera
The ClearVue uses a digital camera to sense the position of the gripper jaws and basket;
this enables the slides to be positioned accurately relative to the gripper jaws. When the
ClearVue is powered on and goes through its initialization routine it takes a picture of
the long jaw and uses it to assess the vertical & horizontal position of the jaw. During
processing the ClearVue also moves each basket into two different positions where
pictures can be taken of the basket. Algorithms in the software then compare the
calculated positions for basket & gripper jaw to calculate how far the basket has to be
moved to line each slide up with the gripper jaws.
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Most of the ClearVue electronics are contained within the electronics box.
This consists of the mains input power supply with 24VDC output; the main
board connected to the processor board via the interconnect board; the battery
charger board and the batteries (for battery backup in the event of a mains
failure).
Some of the motors, solenoids and sensors connect directly to the main board in
the electronics box.
In addition there are four other boards connected to the main board via ribbon
cables. The front interface board and back interface board are used to connect to
some motors, sensors, etc.; the display interface board connects to the touch
screen and some sensors; the camera interface board had the camera and
illumination LED’s.
The mains inlet in the side of the electronics box has a double pole switch and two
fuses (for the live and neutral lines). It also contains a mains filter.
The earth from the mains inlet connects directly to the primary earth stud in the
electronics box.
The power supply has a wide AC input range. The output is 24VDC. There is a
power fail signal. The power supply contains a cooling fan.
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The main board carries out several functions. The power supply section generates
the required voltages from the 24V PSU input (or from the battery input). The
processor board, which plugs into the main board via the interconnect board,
writes to and reads from the FPGA (field programmable gate array) which
interfaces to the sensors and drives.
Conversion from the 24V input (from the power supply or battery backup) to:
15V - used by the battery charger and by the fan drive circuits.
12V - used for the illumination LED’s on the camera board (switched
on the main board). Used by the display board for the screen backlight.
It also goes to the disk drive for drives requiring a 12V supply.
5V for some of the logic and the sensors.
3.3V for some of the logic.
1.2V for the FPGA core power.
Note:
The 24V input from the power supply is fused (F1)
The 24V supply to the motors is switched on only when the processor is
toggling the power watchdog timer (via the I2C bus) and when the
FPGA recognizes that the processor is active. This is used for the motor
drive circuits, the solenoid valves and the pumps.
I2C devices are used to monitor the power supplies. These are read by
the processor.
On the edge of the board are a series of test points and LED’s - these can
be used to check the voltages.
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Note:
Take care if clipping probes to the test points. Avoid applying stress. These are delicate
surface mount test points and can easily be damaged (and may damage the board) if
too much stress is applied. More robust GND connections can be found on the motor
drive IC heatsinks or on the ’D’ connector screwlocks on the main board.
1-4-4-5 - Drives
The solenoid valves, air pumps, fan drives and camera illumination LED
drives are all from the main board. These are all switched by the FPGA
under the control of the processor.
The slotted opto switches and the magnetically operated door switches
are read directly by the FPGA.
The reflective opto sensors used for the coverslip detection feed into an
ADC to be read by the FPGA. They also feed the reference signals
coming from a DAC, controlled by the FPGA, into a comparators. The
outputs from the comparators are used by the FPGA to latch coverslip
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The FPGA controlled the signals to the motor drive circuits under the
control of the parameters sent determined by the processor.
The FPGA controls the DAC’s which provide the reference signals to
determine the current drives and, where appropriate, micro stepping to
the motors.
The motor encoder signals are read by the FPGA and used to derive the
quad positions. These are used by the processor to monitor and control
the operation of the motors.
Note:
The cleaning station motor drive does not have variable current capability or encoder
feedback.
The FPGA controls the data flow from the camera. It saves frames, parts
of which are read by the processor to determine gripper positions, slides
in baskets, etc.
The interface between the processor and the screen is on the main
board.
1-4-4-11 - Links
Directly below the LED’s on the edge of the PCB are the links. The 16-
pin header takes 8 removable links.
Links 1 to 6 are configuration links which are read directly by the
FPGA.
Links 7 and 8 are used for configuring multiple FPGA programming.
On ClearVue all 8 links must be fitted.
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In addition to the removable links there are 2 ’hard links’. These are
fixed links on the PCB. These are used to indicate specific board build
standards.
The status of the removable links 1 to 6, and the two hard links, can be
checked in the Production Services screens.
The 10-pin JTAG, 10-pin PROG and 16-pin TEST headers positioned
below the LINK header are for factory use.
1-4-4-13 - Fuse
There is a fuse for the 24V power input from the PSU.
The interconnect board is a passive board which fits between the processor board
and the main board. The USB connector at the top is not used in ClearVue.
1-4-6-1 - Batteries
There are two 12V 1.2Ah sealed lead acid batteries situated within the
electronics box. These are used to provide power in the event of a mains
power failure. The backup power is only intended to complete baskets of
slides being coverslipped and to ensure that the system stops in a state
where recovery of baskets of slides is simple with the minimum risk of
damage to specimens.
There are two independent multi-stage charging circuits, one for each
battery.
The charge sequence is:
State 1: Trickle charge followed by bulk charge. The trickle charge
ensures that cells within the batteries are not shorted. The bulk charge is
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When mains is present the batteries are in the charging state. When a
mains power fail is detected the relay RL3 is switched to configure the
batteries in series to provide a nominal 24V for battery backup
operation.
When the unit is off, relays RL1 and RL2 disconnect the batteries from
the rest of the circuitry.
1-4-6-4 - Monitoring
As well as the charge state monitoring (see above) and the links (see
below), there is voltage monitoring and temperature monitoring on the
board. The battery isolator switch state is also monitored as is the WGO
signal to monitor power fail / battery backup state.
These monitors are all read by the processor via the serial I2C bus.
1-4-6-5 - Links
LK1 must be fitted in position ’B’. This selects the correct power fail to
battery backup switching state for ClearVue.
Links LK2, 3 and 4 should also be fitted.
In addition there are two ’hard links’ which can not be changed. These
indicate the build level of the board.
1-4-6-6 - Fuses
Each battery has its own fuse on the battery board, F1 and F2.
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There is a speaker connected to the main board. This is driven from the processor
board.
There is a fan within the electronics box. This is driven from the main board. This
has a feedback signal to check fan speed. This fan is always on.
The disk drive connects to the main board but is driven from the processor board.
This is used for loading software updates and for saving engineer logs.
1-4-8-1 - Camera
1-4-8-2 - Interface
The camera interface board, under the control of the FPGA on the main
board, passes the data to the FPGA.
1-4-8-4 - Links
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There are connectors for programming the camera and for analogue
video signals. These are for factory use.
This is a passive board which provides the interconnect between some of the
motors, sensors, etc. and the main board.
This provides the interconnect between some of the motors, pumps, sensors, etc.
and the main board. It also has the three vacuum/pressure transducers used to
monitor the coverslip dispense carriage suction, the coverslip pick-up suction and
the mountant bottle pressure.
1-4-11 - Motors
1-4-12 - Sensors
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The DC solenoid valves control the air flow from the pumps to/from the transfer
head for coverslip pick-up, the coverslip dispense carriage and the mountant
bottle.
In addition a solenoid valve is used to switch the mountant pump syringe between
the dispense head and the mountant bottle.
There is a fume extract fan housed in the top cover. This is driven from the main
board. This has a feedback signal to check fan speed. This fan can be off (when no
filter is fitted) or may run at low speed or high speed depending on the fume
extract requirements.
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2 - Production Services
2-1 - Introduction
2-2 - PARAM Tab
2-3 - I/O Tab
2-4 - MOTOR Tab
2-5 - ENG Tab
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2-1 - Introduction
To access the Production Services screens use the following method:
From the Main Menu press Options.
When in the Disc Options menu press the top-right, bottom-left and top-right of
the touch screen.
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Home
The Production Services welcome screen also displays the Software and FPGA Version
Numbers.
Note:
Pressing the Home Tab from any screen where it is visible will exit the Production Services area.
PARAM
This tab allows the Service Engineer to view and / or change the Parameters.
I/O
This tab shows Sensor Inputs, Switch System Outputs (eg: pumps / solenoids) and
information on Voltages and Battery Backup Status.
MOTOR
This tab allows the Service Engineer to take control of each of the individual motors.
ENG
This tab allows the Service Engineer to perform various tests and checks.
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The top line shows the parameter being edited. In this example its ”Camera Skew
Correct”
The line ”Parameter #0000” tells us which parameter is being edited. In this case
it is parameter 0.
Note:
A full list of Parameters and Settings can be found in Appendix B.
”Value” indicates the current value the parameter is set to.
Pressing the ’I’ key gives a brief explanation of the current parameter.
There are also 8 buttons in the screen. 4 for Adjust and 4 for Parameter.
Under adjust, pressing the S+ or S- keys will increment the parameter value by a
large step. Pressing the ++ or -- key will increment the parameter value by 1. in
both cases + means to increase the value and - means to decrease the value.
The 4 parameter keys work in a similar manner. Press the ++/-- for moving
quickly through the parameter list and press +/- to move in steps of 1.
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This screen shows the status of some of the slotted opto sensor inputs.
’0’ = opto open. ’1’ = opto closed (flag in opto).
This screen shows the door sensors and the filter switch.
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For the doors, a ’0’ indicates door open, a ’1’ indicates door closed.
For the filter switch, a ’0’ indicates filter present, a ’1’ indicates filter not present.
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In this screen its possible to change the condition of various output functions on the
ClearVue. For example, press the ’-’ key to move the cursor down to the ’Bottle pump’
line and press ”1” - this will turn ON the air pump that is connected to the mountant
bottle. Pressing the ’0’ key will switch off the pump.
Press EXIT to return to the main I/O page.
Note:
If the state of any pumps or solenoids are changed, a full initialise must be carried out prior to
using the machine.
See Section 8-2 for the pneumatics diagram.
Mountant pump sol is the solenoid between the syringe and the mountant bottle
or dispense needle.
0 = syringe to dispense needle. 1 = syringe to mountant bottle
Prep solenoid 1 is the coverslip dispense carriage solenoid.
1 = Vacuum to coverslip dispense carriage (Prep sol 2 must be 0)
Prep Solenoid 2 is the support arm suction cup.
1 = Vacuum to the suction cup (Prep sol 2 must be 0)
Bottle Solenoid 1 is the mountant bottle vacuum solenoid. See table below for
settings.
Bottle Solenoid 2 is the mountant bottle pressure solenoid. See table below for
settings.
Bottle Pump is the vacuum pump for the mountant bottle.
Prep Pump is the vacuum pump for the coverslip dispense carriage and suction
cup.
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Prep Solenoid
Status
1 2
No vacuum on carriage or suction
0 0 cup (pump has nowhere to pull air
from; puts strain on the system).
1 0 Vacuum to coverslip dispense
carriage.
0 1 Vacuum to suction cup.
1 1 Partial vacuum on both.
Bottle
Solenoid Status
1 2
0 0 Vacuum to mountant bottle.
1 0 Mountant bottle sealed at solenoids.
0 1 Pump circulates air around closed
loop.
1 1 Pressure on mountant bottle.
On the pneumatics diagram all solenoids are shown connected in their unpowered
state i.e. 0
Note:
Setting both of the prep solenoids to 0 put strains excessive on the pump and the vacuum
regulator and should therefore be avoided.
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There are no features to edit or change in this page, this just shows the status of some of
the I2C bus signals (mainly from the battery board).
PFD: Power fail detection. 0 = OK, 1 = power fail signal detected.
PWR_WDT: Power watchdog. This should normally be ’0’.
pw state: The software power state. ’0100’ is the normal state. ’0110’ is the state
when first running on batteries after a power fail.
OC2, OC1, STL2, STL1: Battery charger states - see below.
LK2, LK3, LK4: Links on the battery board. ’0’ = link present. ’1’ = link missing.
HL2, HL3, HL4: Fixed resistor ’hard links’ on the battery board.
B-SW: Battery isolator switch. ’0’ = switch off, ’1’ = switch on.
WGO: Signal on the battery board indicating the automatic switching of the battery
relays on power fail. ’0’ = normal state, ’1’ = power fail state.
Hard Links:
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In this screen its possible to view all the important voltages within the coverslipper.
The right most column indicates the current voltage seen, with one exception.
The last value is the current temperature within the electronics housing.
Note:
When in power fail mode, Batt2 voltage will be both Batt1 + Batt2 values added together and
shows the voltage available to the coverslipper when no mains is present.
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Shown here are the hardware links and what their status is.
For the normal links ’0’=link present, ’1’=link not present.
The ’hard links’ are fixed resistors on the boards.
These are arranged in four columns as follows:
Column 1
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Link 8
Column 2
Column 3
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NA Not Used
NA Not Used
Column 4
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The STATUS line indicates the current coverslipper status and can be one of the
following.
Unknown
If the coverslipper is yet to initialise or the TAKE CONTROL key has been used
previously, the status will show UNKNOWN.
Idle
Processing
Initialising
Mountant
This will be shown when the user is in the mountant menu pages.
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Malfunction
Note:
Taking control of a coverslipper will force any baskets loaded or processing to stop any further
action. The coverslipper will need to be initialized (when leaving production services via the
HOME tab, if the status is UNKNOWN then a full initialise will start automatically).
In this screen it is possible to take control of the current motor (displayed at the
top of the page).
For the example shown it’s the LOAD motor.
To change to a different motor, press the left and right arrow keys at the top of
the page until the desired motor is selected.
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The box below this indicates the motors' current Quadrature reading and
expected position. In most cases the position should match the quad. A variation
between these two values will indicate a stalling motor or the axis has reached the
end of its current travel. The quadrature value is physically linked to the motor
movement and if the motor is not able to turn, the quad value will remain mostly
constant.
The SET key will set both the current quad and position to 10,000. Press the
STOP key to stop the current motor move.
The four arrow keys below are to perform the motor movements. As a rule, the
arrow points in the direction the motor assembly will travel. So for example the
load rail will move towards the lift if the Right arrow key is pressed. Notice the
two arrow keys at the top are BOLD compared to the lower arrow keys. The bold
arrow keys perform small movements of 50 steps and the lower keys produce
movements of 800 steps.
Also shown on this page are any sensors on the motor axis. So the load page will
show the HOME opto. A '1’ indicates the load opto is being activated.
Press the Release control button to return to the previous screen.
Note:
Clashes between mechanisms can occur when moving motors from these screens. Take care when
moving any motors in this way as damage can occur and can lead to errors when initialising.
Load rail The load mechanism can clash with the lift block.
motor
Lift motor Clashes can occur between the vertical lift and the load rail, the eject
bracket, the grippers (if a basket is loaded) and the basket open
mechanism (if a basket is loaded).
Coverslip The transfer head can clash with the coverslip dispense, the support
transfer head arm, the grippers and the eject bracket. When rotated clockwise the
head position pin (flag) can be damaged by hitting the lower crank
arm.
Basket slider This could collide with a basket on the lift block.
open
Basket eject The eject bracket can clash with the support arm, the transfer head
motor and the vertical lift block.
Coverslip The coverslip dispense, support arm and dispense head needle can
dispense clash with the transfer head, the grippers, the eject bracket and the
motor cleaning station pot (always move the cleaning station pot to the
bottom position before moving the coverslip dispense motor).
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Slide The grippers can clash with a basket on the lift (if loaded), the transfer
gripping head and the dispense head.
motor
Mountant Excess pressure can be generated in the syringe if the dispense needle is
pump motor blocked. Take care, excessive quantities of mountant may be dispensed
into the cleaning station pot or elsewhere in the machine.
Cleaning Clashes can occur between the cleaning station pot and the dispense
station motor head.
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Note:
Some of the keys on these pages may change depending on the latest version of the software.
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ProdTst1
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
If this menu is entered the load rail will need initializing as soon as the menu is
exited. Baskets will not load if it is not initialized.
ProdTst2
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
ProdTst3
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
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ProdTst4
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
ProdTst5
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
To Run ProdTst5:
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To increase the number of pixels between the slide and gripper, increase the
number on the screen.
Note: This will not alter the picture on screen, but will change the relationship for the next
slide selected.
If the number of pixels between the slide and grippers is correct use the next key
to confirm a minimum of 5 slides meet the requirement
Press accept and wait for the basket to complete.
Press exit to return to production services.
Put "Prep" back to "1"
Note: If using Leica or V24 baskets, load the baskets fully but ignore the first 5 and the last 5
slides.
Note: Additional baskets of slides may be added if necessary.
Note: Screen corruption may occur if keys are pressed as the camera image is being drawn.
Note: Switching off and restarting the instrument will clear this error.
ProdTst6
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
To Run ProdTst6:
Prepare a basket of 20 dry slides, ensure a good hopper with adequate quantity
and quality of coverslip is in place and then select / do the following:
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ProdTst7
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
Do not enter this menu unless you have been specifically instructed
to do so. If you do enter by mistake with the wrong software version
the machine will need to be switched off by the battery which will
loose any changes made to setting and may corrupt the machines
files.
Results
First asks for the serial number of the ClearVue then copies the results to a floppy
disk.
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Pnumat 1
MAX PRESS - Pressurizes the mountant bottle, up to 10psi, and shows the
maximum pressure reached. This will be a PASS if 10psi is achieved within 2
minutes, otherwise it will show a FAIL.
MIN PRESS - Pulls a vacuum on the mountant bottle, down to -6psi, and records
the lowest vacuum reached. This will be a PASS if -6psi is achieved within 2
minutes, otherwise it will show a FAIL.
LEAK TEST - Pressurizes the mountant bottle, up to 10psi, and holds the pressure
to detect air leaks in bottles / fittings etc. This will be a PASS if the pressure is still
greater than 7psi after 2 minutes, otherwise it will show a FAIL.
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Pnumat 2
SET VAC - Pulls a vacuum on the transfer head and shows the current reading in
PSI. Adjust the vacuum regulator to the required value.
DECAY - Releases the vacuum on the transfer head via the restrictor and times the
decay.
Pnumat 3
Accessing this page will give information about the vacuum that is available on the
coverslip carriage during a coverslip dispense.
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Press the RUN key to dispense a coverslip and show the graph.
Press CLR to clear the current graph or EXIT to return to the previous menu.
Remove the coverslip and ensure the vac level drops to ambient level in less than 1
second.
PREP
Prep key.
Toggles between '0' and '1'. The value shown on the key is its current state.
This switches the mountant dispense on and off.
When set to '0', there is no coverslip dispense or mountant dispensed. The slide
will be taken out of the basket and returned.
When set to '1' it operates normally.
This can only toggle when the machine is idle.
This is reset to '1' during a full initialise.
This function can be used for testing gripper alignment, basket handling, slide
handling etc, without using mountant and coverslips.
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testslip
QUAD A/B/C/D - Latched quadrature positions of slip dispense when the optos
detected the slip on the carriage during a dispense. Note that on dispense of a good
slip all values may appear the same in testslip. On a bad dispense, actual latched
values may be visible.
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OPTIC 1 and OPTIC 2 - Current value being read from the slip dispense optos
(in realtime). Lower readings indicate a slip (typically 200-600). Each optic floats
around the value 1300 to 1400 with no slip.
Beads
Beads key.
Toggles between '0' and '1'. The value shown on the key is its current state.
When set to '0', it operates normally.
When set to '1' a bead of mountant is dispensed onto the slide but no coverslip is
used.
This can only toggle when the machine is idle.
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Dunks
This is a factory test. Do not run this test unless specifically requested to do so by the
factory or unless directed to do so from within this manual.
Toggles between '0' and '1'. The value shown on the key is its current state.
When set to '0', it operates normally.
When set to '1' the cleaning station is used to clean the mountant dispense needle
after every slide.
This is reset to '0' during a full initialise.
EMC RUN
This activates a special continuous running programme used for factory test
purposes only. This routine should not be used. Clashes may occur between the
dispense needle tip and the gripper arms. This could cause damage to the parts
involved.
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dump log
clearlog
params ->
->params
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profile
Shows the current profile version, profile creation date and checksums.
The checksums should match.
The import key can be used to load a new profile from a floppy disk.
Note:
After importing a new profile a full initialise should be carried out before using the machine.
ProdTst8
This is a factory test. Do not run this test unless specifically requested to do so by
the factory or unless directed to do so from within this manual.
reset 7
exit2dos
Switches off the ClearVue and drops to a DOS prompt (regardless of whether
processing or not).
This function can be used to save changes to parameter files when the ClearVue
will not power down correctly. Everything is saved as is for a normal shut down.
This function is only useful to access the files on the flash drive with a monitor
and keyboard attached.
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Note:
Do not carry out any initializations while ClearVue is processing baskets.
fullinit
i-input
i-open
i-lift
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i-npot
i-slip
i-prep
i-eject
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Note:
Do not carry out any initializations while ClearVue is processing baskets.
i-grip
Note:
Grippers can clash with a basket on the lift block, the dispense head and coverslip transfer head.
Ensure all assemblies are clear before pressing 'init'.
i_pump
i_camera
Note:
This may give an incorrect gripper position if the grippers have been moved since last gripper
initialization.
TFT BLIT
This causes every image from the camera to be displayed on the screen.
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snapshot
The crosshairs indicate the detected corner of the gripper at the last initialise.
Note:
This is a negative image.
cam info
This screen shows information regarding the camera image analysis, including the
last basket top positions, the x and y positions of the gripper jaw, etc.
Notes:
The 'cam focus clean’ value is an indication of any blurring of the image. A value of 5 or more
may indicate that the lens needs cleaning.
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'Cam status bits' are: Link 5, Camera type, Camera Ready, Digital mode. These are only valid
after the camera has been initialised and are for Engineering use only.
qds/pos
This shows the quadrature feedback and drive positions as well as the current speeds
of the various motors.
states
The information on this screen may be requested by the factory during problem
diagnosis.
The first column indicates the software state (5-digit number). When ClearVue is
initialised, all of these should be '00100' except for 'Cam' which should be
'00220'.
The second column indicates the status (2-digit number).
For the mountant pump, 'dr' is the number of dispenses remaining in the syringe
based on the current settings.
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'Baskets' is the number of baskets detected on the load rail including any on the
lift.
'lift loaded' indicates whether a basket is currently on the lift.
'ST' is the overall machine status.
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ms menu
Note:
The machine will initialise when this menu is exited to the home screen.
This menu has keys for:
Flush
Purge
Volume
De-gas
Access
This allows use of all of the mountant systems functions from within production
services. A flush can be carried out with the covers off the machine to check for air
in the system.
AutoSlip
The AutoSlip routine sets the optical thresholds for the coverslip detection
(including double coverslip detections). The thresholds are stored in the parameter
file.
Before running this test ensure that a good coverslip hopper is installed and that
coverslips can be dispensed.
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Note:
High ambient lighting will invalidate the test. Where possible run with the covers on or ensure
no direct light is in the area.
The new values are only used when the next initialise of the coverslip dispense occurs.
The parameter file is saved when the machine is powered off. If the machine can not power
down correctly these values may be lost.
Beadopt
When 'beads' is set to '1' (ENG Tab, page 2) this can be used to test the bead size
and position.
jawalign
This can be used to check the alignment of the gripper jaws to the basket. This is for
factory use only.
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5minmode
This function temporarily disables all the safety interlocks on ClearVue and should only
be used by trained personnel. ClearVue should never be left unattended whilst in
'5Minmode'.
slip QC
INIT initialises the coverslip dispense system. This also loads the coverslip opto
parameters if they have been changed since the last initialise (such as when using
AutoSlip).
RETRY, when OFF the coverslip dispense will halt as soon as an error is detected.
When RETRY is ON the coverslip dispense will attempt a number of retrys (as set
in the parameters).
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The box at the bottom of the screen is the description of the last error detected.
2. No coverslip dispensed
5. Length error
7. Skew detected
There is a risk of static shock when performing this test - Ensure the
debris tray is suitably earthed before commencing test.
dump scr
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Activation of this feature may cause changes to settings in the ClearVue. If this is
accidentally run, check all settings.
ClearPST
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LifeTest
This is for factory use and should only be used if directed to by the factory or this
manual.
Prep Rotate
This test can be used to check the coverslip transfer head hard stop setting.
Check the machine has initialised correctly and fully.
Remove the covers from the machine, including the right hand side cover.
Put a magnet on the debris door only, hold in place with tape as required (You
can use the I/O tab - INPUTS Section 2-3-1 to check the magnet is in the
correct place).
Push the grippers into the prep area and place a slide in the jaws.
Remove the suction cup and use a bulldog clip or crocodile clips to hold the
sprung pads up out of the way.
Press 'Start'.
The prep head will move over to the slide and stop in the final position of prep
(if it continues on, the magnet on the prep door is not in the correct place).
The prep head should just touch the slide without bending it. If it stops above
it should be less than 0.1mm above the slide.
Repeat the test (press start again) to confirm that it is not bending the slide.
If the head bends the slide or stops more than 0.1mm above the slide, then
reset the Coverslip transfer head hard stop, see Section 3-4-10.
Note:
Always re-initialise the machine after changing the hard stop before repeating this test.
X-spot
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UIFReset
This option returns all user settings to their default values (for example: coverslip
position, screen angle, mountant dispense volume).
Bmp Dump
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3 - Functional Tests
3-1 Earth Continuity and Insulation Tests
3-2 Individual Motor Tests
3-3 Build Subassembly Tests
3-4 Instrument Verification Tests
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These tests should be carried out without any baskets loaded onto the instrument.
Take particular care that different mechanisms do not clash and cause damage (see
Section 2-4).
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From the TAKE CONTROL screen scroll to the LOAD motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'right arrow' keys - check that the load rail mechanism moves to
the right and that the QUAD and POSITION values increase.
Press one of the 'left arrow' keys - check that the load rail mechanism moves to
the left and that the QUAD and POSITION values decrease.
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From the TAKE CONTROL screen scroll to the LIFT motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'down arrow' keys - check that the lift mechanism moves
downwards and that the QUAD and POSITION values increase.
Press one of the 'up arrow' keys - check that the lift mechanism moves upwards
and that the QUAD and POSITION values decrease.
Towards the top of the travel the basket slider closing mechanism is engaged. This
can collide with the basket eject mechanism. This should be avoided as damage
can occur.
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From the TAKE CONTROL screen scroll to the PREP-R motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'left arrow' keys - check that the transfer head moves to the left
(anti-clockwise) and that the QUAD and POSITION values increase.
Press one of the 'right arrow' keys - check that the transfer head moves to the right
(clockwise) and that the QUAD and POSITION values decrease.
Take care not to stall the motor by hitting the head position pin on the lower
crank arm when rotating to the right (clockwise). The pin can be damaged.
When rotating left (anti-clockwise) ensure that the mechanism does not hit the
mountant dispense arm or the grippers.
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From the TAKE CONTROL screen scroll to the BASK OPEN motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'down arrow' keys - check that the basket open mechanism moves
towards the front of the machine and that the QUAD and POSITION values
increase.
Press one of the 'up arrow' keys - check that the lift mechanism moves towards the
rear of the machine and that the QUAD and POSITION values decrease.
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From the TAKE CONTROL screen scroll to the EJECT motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'down arrow' keys - check that the basket eject mechanism moves
to the left and that the QUAD and POSITION values increase.
Press one of the 'right arrow' keys - check that the basket eject mechanism moves
to the right and that the QUAD and POSITION values decrease.
Care should be taken when moving the basket eject motor. Damage may occur if
the basket eject mechanism collides with the slider closing mechanism (when the
lift block is towards the top of its travel), the prep head or the dispense arm
(particularly the mountant pipe).
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From the TAKE CONTROL screen scroll to the N_POT motor screen.
Press one of the 'down arrow' keys to move the cleaning station mechanism until
it is clear of the mountant dispense needle. Failure to do so may result in damage
to the needle.
Scroll back to the SLIP DISP motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'up arrow' keys - check that the coverslip dispense mechanism
moves towards the rear of the machine and that the QUAD and POSITION
values increase.
Press one of the 'down arrow' keys - check that the coverslip dispense mechanism
moves towards the front of the machine and that the QUAD and POSITION
values decrease.
The coverslip dispense mechanism, support arm and dispense needle can clash
with the prep head, the basket eject mechanism, the gripper arms and the cleaning
station pot and cause damage.
Ensure that these are in appropriate positions before moving this motor.
After the test has been carried out, the cleaning station (filled with xylene) should
be moved back into position to prevent the mountant in the dispense needle from
drying out. This is most easily carried out by going to the production services
ENG tab, page 4 (Section 2-5-4) and pressing the 'i-slip' key to initialise the
coverslip dispense system (including the cleaning station).
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From the TAKE CONTROL screen scroll to the GRIPPERS motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'right arrow' keys - check that the grippers move to the right and
that the QUAD and POSITION values increase.
Press one of the 'left arrow' keys - check that the grippers move to the left and that
the QUAD and POSITION values decrease.
Take care that the gripper arms do not collide with the prep head or the
mountant dispense needle. Damage may occur.
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From the TAKE CONTROL screen scroll to the M_PUMP motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
Set the power to 64 (maximum).
Press one of the 'down arrow' keys - check that mountant pump mechanism
moves downwards and that the QUAD and POSITION values increase.
Press one of the 'up arrow' keys - check that the mountant pump mechanism
moves upwards and that the QUAD and POSITION values decrease.
The movement can only be seen if the covers are removed.
If the covers are fitted, the same test can be carried out to check that the QUAD
and POSITION counts change correctly, however to verify that mountant is
being dispensed, using the I/O tab, OUTPUTS (Section 2-3-2) set the 'Mountant
pump sol' to '1', next lower the cleaning station then move the coverslip dispense
mechanism to place the mountant dispense needle above the purge tray. Now, at
the M_PUMP motor screen use one of the 'down arrow' keys and observe the
mountant being dispensed out of the needle.
To refill the syringe set the 'Mountant pump sol' to '0' and drive the dispense
mechanism upwards.
Once finished, initialise the coverslip dispense mechanism using 'i-slip' (Section 2-
5-4) to ensure that the needle is left in the cleaning station to prevent the
mountant from drying out.
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From the TAKE CONTROL screen scroll to the N_POT motor screen.
Press 'Set' and check that the QUAD and POSITION values are set to 10000 and
are not changing.
This motor runs at fixed power - changing the power setting has no effect.
Press one of the 'up arrow' keys - check that cleaning station mechanism moves
upwards and that the QUAD and POSITION values increase.
Press one of the 'down arrow' keys - check that the cleaning station mechanism
moves downwards and that the QUAD and POSITION values decrease.
When moving the cleaning station in an upwards direction, ensure that it will not
hit the mountant dispense needle - damage may occur.
After the test has been carried out, the cleaning station (filled with xylene) should
be moved back into position to prevent the mountant in the dispense needle from
drying out. This is most easily carried out by going to the production services
ENG tab, page 4 (Section 2-5-4) and pressing the 'i-slip' key to initialise the
coverslip dispense system (including the cleaning station).
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Stage 1 - Initialise
During this stage the speaker connection is checked, all of the motors and opto
switches are used and the camera illumination is also checked.
During this stage the doors and filter switch are checked, the fans are checked, the
battery backup system is checked, the voltage regulators are checked, the links on
the PCB's are checked, the slip dispense opto's are checked and the camera
operation is checked.
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With the filter in place and the unload door closed, the filter fan should show
greater than 50Hz.
When the unload door is opened the speed should increase to greater than
80Hz.
Go to the I/O Tab, I2C screen (Section 2-3-3).
Check that PFD shows '0', PWR_WDT shows '0', pw state shows '0100' and
WGO shows '0'.
Check that B-SW shows '1' when the battery switch is on and that this is '0'
when off.
Make sure that the battery switch is on.
Switch the mains off.
Check that PFD shows '1', PWR_WDT shows '0', pw state shows '0110' and
WGO shows '1'.
Note:
These signals must be checked immediately after switching the mains supply off.
The PFD and WGO signals are only valid for approximately 5 seconds after which their
states are indeterminate and either of them may show '1' or '0'.
Switch the mains back on, check that the signals return to the correct state. It
may take a few seconds for 'pw state' to change from '0110' back to '0100'.
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Note:
The battery voltages are dependant on the charge state.
Batt2 voltage will show Batt1+Batt2 (nominal 24V) during mains power fail.
With the battery switch in the on position, switch the mains off. Check that
Batt2 changes to at least 20V.
Switch the mains back on and check that Batt2 returns to a normal reading.
Go to the I/O Tab, LINKS screen (Section 2-3-5).
Check that the links are as shown:
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Note:
For issue 1 battery boards, the battery board hard link 2 will be '1'.
Go to the ENG Tab, Page 2/7 (Section 2-5-2).
Select 'testslip'.
Press INIT.
Insert a hopper of coverslips.
With no coverslip under the sensors, check that the 'Optic 1' and 'Optic 2'
readings are between 01200 and 01500 and that 'Logic 1' and 'Logic 2' are
both '1'.
Press HALF OUT. Check that a coverslip is under the sensors. Check that the
'Optic 1' and 'Optic 2' readings are between 200 and 1000 and that 'Logic 1'
and 'Logic 2' are both '0'.
Press RETURN. Check that the slip is cleared.
Press SLIP OUT. Check that a 'Good slip' is indicated.
Go to the ENG Tab, Page 5/7 (Section 2-5-5).
Select 'snapshot'.
Check that the image is square and that the gripper jaw is positioned in the
right hand side of the screen.
During this stage the connections to the disk drive are checked.
During this stage the operation of all of the solenoids (including the mountant
dispense) are checked and the pressure sensors are checked.
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Remove the coverslip dispense skirt and clean the rubber section. Check for
damage and replace if required.
Check the coverslip dispense skirt is free moving in its slot.
Check the height of the end stop. Refer to Section 3-4-11.
Inspect end stop for signs of wear and damage. Bottom edge should be sharp.
Check carriage for mountant contamination or build up of dirt, clean if necessary.
Ensure that all three of the vacuum ports in the coverslip dispense carriage are
clear from debris.
Clean glass dust out of the coverslip dispense carriage and banjo fitting.
Check the coverslip dispense carriage hard stop for signs of wear or damage. All
corners and edges should be sharp.
Inspect the coverslip dispense system and surrounding area for debris clean as
necessary.
Check the vacuum pipes and glass filter for blockages and leaks.
Check the pulley on the coverslip dispense motor is secured to the motor shaft.
Inspect the coverslip dispense timing belt for damage and signs of wear.
Check the optical sensor wires have not been snagged or damaged by either the
coverslip dispense cover or ribbon cable cover.
Check the belt tension - see Section 5-4-12.
Check the optical sensors have been set correctly - see Section 3-4-17.
Check the optical sensors are not contaminated with glass dust, clean the sensing
faces of each with a long bristle brush reset them - see Section 3-4-18 - and run
Autoslip.
Check Parameter 59 and 60 are set correctly by running Autoslip - see Section 3-
4-17.
Carry out motor check - see Section 3-2-6.
Check coverslip dispense optical switch by initialising and checking the dispense
head needle is central in the cleaning station.
Carry out Performance Test 3.
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Remove the mountant dispense needle - see the Operator Guide Section 5-7.
Check for any mountant build up on the end of the needle and clean if required.
Check for any mountant build up on mating surface between the dispense needle
and arm and clean if required.
Check that the dispense needle end is still circular and has not been damaged.
Check the mountant line is not damaged and that the nipple connector is tight
and secure.
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Remove the coverslip transfer head - see Operator Guide Section 5-12.
Check for any mountant build up, splitting or fraying of the suction cup.
Check for any mountant build up and for full free travel of the sprung pads, by
gently pushing at either end and the centre of the pads - both pads should move
freely and smoothly.
Check that the pneumatic line is not damaged and has a clean cut end.
Check the height of the suction cup and sprung pads as shown.
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If the suction cup height is wrong, ensure it is pushed home fully or replace as
necessary.
If the sprung pad heights are wrong, adjust the locking nuts on top.
If the sprung pads are catching or sticking clean off any mountant and lubricate
the pins with silicon lubricant or microtome oil.
Note:
Keep all surfaces of the pads clear of lubricant.
The coverslip transfer head will be one of the following colours:
Silver - Standard Head - Early builds with lower tolerances
Black - Standard Head - Current build with improved tolerances
Blue - Offset coverslip transfer head - see the Operator Guide Section 3-11
Note:
If repositioning a silver coverslip transfer head, or removing to swap with another colour, see the
Operator Guide Section 3-3.
After changing a coverslip transfer head, it is important to check the position of the hard
stop (see Section 3-4-10).
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When looking at the back of the machine the LEFT solenoid controls the
Vacuum side of the circuit.
The RIGHT solenoid controls the pressure side of the circuit.
The LEFT solenoid connector is on the top.
The RIGHT solenoid connector is on the bottom.
On this manifold both the central fittings are connected directly to the pump.
Both the pressure and vacuum side of the pump are connected.
This is the pump towards the rear of the machine.
Slip Pneumatics:
When looking at the back of the machine the LEFT solenoid controls the Slip
Placement.
The RIGHT solenoid controls the vacuum on the slip dispense.
Both solenoid connectors are on the top.
On this manifold both the central fittings go to the slip dispense/ placement,
via a filter and sensor.
Only the vacuum side of this pump is connected.
The pressure side has a silencer to reduce noise.
The pump goes to the slip dispense solenoid directly and via the regulator to
the slip placement solenoid.
This pump is towards the front of the machine.
Connected to the left hand side of the solenoid is a pipe that leads to the decay
reservoir and then the variable restrictor, which controls the speed at which the
vacuum on the slip placement is released.
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If issues arise, check for leaks and blockages in the system before altering this
setting.
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3-4-10 - Checking and Adjusting the Coverslip Transfer Head Hard Stop
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Check that the time and date are correct on the instrument - change them if
necessary (see Operator Guide Section 3-5).
Load a basket with slides.
Choose volume 13 from the mountant menu (see Operator Guide Section 3-9).
Run the basket through as normal, but remove it as soon as it is finished.
Note:
Ensure the slides remain level as they may slip if they are tilted.
Lay the slides out flat as quickly as possible, taking care not to disturb the
coverslips.
Check the coverslip alignment against the following criteria:
The coverslip should not overhang the slide at any point.
The coverslip should be central from left to right as shown.
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There will be some overspill at the top and bottom ends of the coverslip.
There should be little or no overspill on either side of the coverslip.
There should be no witness marks on the coverslip caused by the suction cup or
transfer head sprung pads.
There should not be a line of mountant on the frosted portion of the slide.
There may be a small bubble towards the non-frosted end of a few of the slides.
Ensure there are no bubbles over 1mm in diameter.
Ensure no single slide has multiple bubbles.
Extract an Engineers Log and open it using either the default Notepad Windows
application, or as an Excel spreadsheet, using the ClearVue Macro to manipulate
the data.
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Locate the time and date that the basket was processed.
The file format will be similar to the extract below:
qc:10469 qd:10469 5
er_no:00
Note:
The shaded section at the end of each line has been added as a point of reference for
this manual.
It will NOT be shown on an actual engineers log.
The date is shown in the format MM/DD/YYYY and the time is in hh:mm:ss.
When the correct time and date have been located, look for 'Slide No:01' (see
end of Line 1) which is the first slide in a basket.
Then locate the corresponding 'Prep Decay Time' (see Line 9) for the slide.
The time should be between 2 and 4 seconds.
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Repeat this for all 20 slides (Slide No:01 to Slide No:20) and ensure they are
all within the correct range.
Anything with a Prep Decay Time of < 1.5s will result in a large number
of bubbles and unacceptable Prep Quality!
Locate the date and time that the basket was processed.
The date is shown in the format DD/MM/YYYY and the time is in hh:mm:ss.
There will be 20 prep decay times listed for the basket.
All the prep decay times should be between 2 and 4 seconds.
Anything with a Prep Decay Time of < 1.5s will result in a large number
of bubbles and unacceptable Prep Quality!
Further details on analyzing the Engineers Log produced by the ClearVue
Macro can be found in Appendix C.
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Performance Test 2 is designed to ensure that the ClearVue can handle slides and baskets
without any issues.
Method
Interpreting Results
If the ClearVue is operating normally it should process all 5 baskets with no errors
and no broken slides.
Note:
A small number of errors putting the slides back into the basket are inevitable, therefore 1 user
error 6 (engineering error 91) is acceptable during the 10 baskets.
Open the log file taken (see Appendix C) and check for any errors or warnings
during the processing of the baskets.
Check the log file (see Appendix C) for the consistency of the 'auxiliary cam init'
values, the values should not vary by more than 1 pixel over the course of the 10
baskets.
Check the log file (see Appendix C) for the consistency of the 'basket top1' values,
the values should not vary by more than 2 pixels over the course of the 10 baskets.
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There is a risk of static shock when performing this test. Ensure the
debris tray is suitably earthed before commencing the test.
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The 'Pnumat 1' menu tests the pneumatics that pressurise the bottle to prevent
cavitation during loading of the syringe and pulls a vacuum to de-gas the
mountant, pulling excess air out of the mountant.
Use 'Pnumat 1' test - see Section 2-5-2 - to check mountant bottle pneumatic
system.
Complete each test, Max Press, Min Press and Leak Test in order.
Details of Pass / Fail criteria are given in Section 2-5-2.
Repeat the Max Press test, but when the pressure reaches 5PSI, open the
mountant bottle lid to quickly release the pressure.
Check that the pressure on screen rapidly falls to 0PSI (±1PSI).
To check the bottle seals, remove the pneumatic line from the bottle and use a T
piece to blank off the end.
Repeat the Leak Test and compare the results to the Leak Test with the bottle
attached.
Each solenoid can be checked by using the I/O Tab - OUTPUTS to switch the
solenoids on and off.
See Section 2-3-2 for details for how to set each solenoid.
I/O Tab - INPUTS, page 2 can be used to monitor the vacuum.
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The 'Pnumat2' menu tests the vacuum level and decay time for the coverslip
transfer head.
Use the 'Pnumat 2' test in Section 2-5-2.
Complete each test, Set Vac and Decay in order.
First, remove the pneumatic line from the coverslip transfer head and place it in
the blank fitting (bottom right of the vertical plate).
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Press the 'Set Vac' button and leave the vacuum to settle for 30 seconds. Check
the maximum value and ensure that the recorded value is -4.5psi, ±0.5psi. See
Section 5-4-8 for details on replacing the vacuum regulator.
To adjust the regulator, open the cap by pulling away from the mounting bracket.
The cap can now be turned to adjust the vacuum level.
Push the cap closed after adjustment to prevent the settings from creeping.
Press 'exit' to stop the test.
Press the 'decay test' button to check the vacuum decay time.
The decay should be 3 seconds, but the screen will record a pass if the decay time
is between 2 and 4 seconds.
To alter the vacuum decay time use a 2.5mm allen key to adjust the exhaust
restrictor, turn clockwise to increase the decay time. See Section 5-4-7 for details
on the exhaust restrictor.
Return the pneumatic pipe to the coverslip transfer head and press exit to leave
the test menu.
Each solenoid can be checked by using the I/O Tab - OUTPUTS to switch the
solenoids on and off.
See Section 2-3-2 for details for how to set each solenoid.
I/O Tab - INPUTS, page 2 can be used to monitor the vacuum.
This can be completed either with the pneumatic line connected to the blank
fitting, or a coverslip can be used on the suction cup.
Always check the decay time that is set here, corresponds to the actual decay time
when processing baskets - This can be done by analysing the Log file.
See Section 3-4-1 for details of how to analyse the Log file.
If the decay set here is greater than the actual decay time in the log file, there may
be a fault with the suction cup or the connection between the pneumatic line and
the coverslip transfer head.
See Section 3-3-9 for details on checking the coverslip transfer head.
Initialise the coverslip transfer head by choosing I-Prep, see Section 2-5-4.
Watch as it initialises. You should see both sprung pads touch the coverslip
dispense carriage.
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If only one touches, the system is stalling too high and hence the coverslip transfer
head will not be able to come low enough to pick up the coverslip.
See Section 3-3-9 and Section 3-3-10 for details on checking the assembly.
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Check that both pads are free to move to the limits of their travel.
Use feeler gauges to ensure that the gaps between the main body of the transfer
head and the sprung pads are the same as those shown above.
If it is necessary to adjust the pads, do so using the Nylock nuts on the shoulder
above the pads.
Turning the nut through 1 revolution will raise or lower the sprung pad by 0.5
mm.
Turning the nut through 1 flat of the nut (60°) will raise or lower the sprung
pad by 0.083 mm.
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Ensure the covers have been removed along with the camera cover.
Detach the dispense head from the support arm.
Remove the gripper return plate and the jam indicator finger from the short jaw
arm.
Assemble the DTI and bracket, and then screw it to the support arm as shown.
Note:
Ensure the large hole in the bracket fits over the boss on the support arm.
Fix the gauge plate to the short gripper arm as shown using the cap-head screw
provided
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Remove the DTI and bracket from the support arm and fix it to the lower linear
rail as shown.
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Note:
The fixing screw from the linear rail must be removed to allow the hole to be used for the
bracket.
Bring the jaws together so that they are end-to-end.
Manually raise or lower the end of the long gripper jaw until the tip is within
0.1mm of the height of the tip of the short jaw.
Note:
The tip of the long gripper jaw must be lower than the tip of the short gripper jaw (to
within tolerance).
Position the DTI probe on the upper surface of the long gripper jaw and manually
move the jaw to determine the run-out.
The tip-to-tail variation must be within 0.2mm.
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If the run-out is greater than 0.2mm the tail end of the long gripper jaw can be
raised or lowered using the height jacking screw.
The fixing screws should be slackened off to allow the adjustment and then
tightened to 3Nm when the adjustment is complete.
Note:
When jacking the tail-end of the long gripper jaw, it is advisable to hold the tips of both
jaws together to ensure that the previously set tip does not move too much.
After adjustment, re-check the tip-to-tip variation to ensure it is still within
tolerance.
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Push the long gripper jaw to the right and bring both jaws into prep position.
Place a slide between the grippers in the correct position. Check that the slide is
held securely and does not wobble.
Push the cleaning station down so that it clears the dispense head needle.
Push the dispense head towards the prep area so that the needle is just behind the
grippers (See Figures 1 & 2).
Using feeler gauges check the height of the needle above the slide. The correct
value should be between 0.70 and 0.75mm at the gripped end.
Repeat the above test at the far end of the slide and confirm it is in the range of
0.60 - 0.80mm.
Note:
Be aware that the feeler gauge can bend the slide down especially at the far end of the slide
and this could give you a false reading.
If the run out along the slide is greater than 0.2mm then the gripper alignment
must be reset - See Section 3-4-8.
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With the needle locking screw still tight, use a 10mm spanner (wrench) on the
adjustment height screw to achieve the correct height. When viewed from above,
turning the needle clockwise will reduce the needle height.
Loosen the needle locking screw and allow the whole needle assembly to rotate to
un-twist the mountant pipe.
Re lock the needle in place, check it is secure and recheck the needle height at
either end of the slide.
Ensure that all mating faces are free of mountant or other debris and that the
needle sits correctly in the arm.
Note:
Do not over tighten the needle locking screw as customers need to be able to remove the
needle without tools.
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3-4-10 - Checking and Adjusting the Coverslip Transfer Head Hard Stop
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Lift the transfer head sprung pads and retain in this position with crocodile clips
or adhesive tape.
Bring gripper jaws into prep position.
Place a slide into the gripper jaws and check that it is held in place securely.
Move slide forward into a position where it is under the transfer head but not the
sucker.
Move transfer head over until it is just touching the slide; being careful that head
is just touching and has not bent the slide or changed its position in the jaws.
Using feeler gauges check the gap between the lower crank arm and the transfer
head hard stop.
Correct gap should be 0.5mm.
Note:
The LifeTest button in the ENG Tab, page 7 (see Section 2-5-7) can be used as an
alternative method of checking the hard stop setting.
Slacken the single M6 retaining screw in the hard stop until it is finger tight.
Using a flat blade screwdriver rotate the hard stop until a good fit is felt with
the feeler gauges.
Simultaneously tighten the retaining screw to a torque of 5.5Nm whilst
holding the hard stop in position with the screwdriver.
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Always re-initialise and recheck the gap after tightening the screw.
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The Software and FPGA version numbers are shown at the top of the screen.
The Pump Profile version (Pr) is shown at the bottom-left of the screen.
If the version numbers are incorrect, refer to Section 5-5.
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Note: Any user adjustment of parameter 72 (slide visibility threshold) will be lost after a reset 7.
Make note of the value of parameter 72 before starting, and change after reset 7 if required.
To reset the parameters choose reset 7, see Section 2-5-3 - ENG Tab - Page 3/7
Wait for the system to finish initialising.
Then complete the following tests to reset the parameters.
Section 2-5-1 - ENG Tab - Page 1/7, ProdTst5
Section 2-5-1 - ENG Tab - Page 1/7, ProdTst6
These 2 tests will reset the relationship between the slide to the gripper jaws and
the coverslip position on the slide. These settings are unique to each instrument.
Confirm the user settings are correct for the customer as per the quick set up
guide.
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Using the door sensors screen in Production Services - I/O Tab - INPUTS - see
Section 2-3-1 - open each door in turn.
A '1' on the screen indicates that the door is closed.
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Confirm whether the user has selected the End or Central position for the
coverslip transfer head in the user setting. Note that if using the Offset Coverslip
Transfer Head, either of these positions can be selected to the same effect (see
Operator Guide Section 3-2).
Confirm that it corresponds with the actual position of the coverslip transfer head.
Follow the instructions below to check if the flag on the coverslip transfer head is
breaking the sensor appropriately.
Use the inputs screen, see Section 2-3-1 - I/O Tab - Inputs.
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Remove the main cover and then the 3 screws securing the sensor cover.
With a hopper in place press slip 'Half Out' Slacken the M3 screws securing the
sensors to the hopper mount then move the sensor up and down until the reading
for that sensor is at its lowest. (must be below 950) then tighten the screw, repeat
for the other sensor.
Press 'Return' then 'Init'.
Exit from 'Test Slip'.
With a hopper in place press 'Autoslip' - exit from Autoslip when it is finished.
Go to slip QC - change to continuous running and ensure that a quantity of 20
can be dispensed without errors.
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There are two ways to complete a bead test: the User Bead Test method and the
Advanced Bead Test method.
This method either lays down the default bead (50mm coverslip, end position) or it will
lay down the bead suitable for the last coverslips that were put through. Note, if you have
just switched the machine on or cleared the coverslip count on the front screen it will
return to the default setting.
To access the user bead test, do the following starting from the Main Menu screen:
Press 'Options'.
Press 'Slip pos'.
Open all 4 access doors.
Place a basket of dry slides on the input load rail, but do not close the load door
yet.
Touch the coverslip shown in the diagram on the screen.
The coverslip will disappear and you will hear an ascending musical tone.
Close all of the doors and let the machine process the basket of slides.
When finished, remove the basket and lay the slides out flat.
Note:
The mountant will run while it is still liquid, so ensure the slides are kept flat at all times.
See Section 4-4-6 for details on interpreting Bead Test results.
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This method gives the option to choose the bead size and position.
Open ENG Tab, page 2 (see Section 2-5-2) and press the Beads button. The value
shown on the button should be '1'.
Open ENG Tab, page 6 (see Section 2-5-6) and use 'Beadopt' to select the slip size
and position of the bead.
Load a basket of slides and begin processing.
Mountant will be dispensed onto the slide, but no coverslip will be placed.
When the basket has finished processing the formation of the beads can be checked
to allow problems to be diagnosed.
See Section 4-4-6 for details on interpreting Bead Test results.
Note:
Ensure processed slides are kept flat at all times to avoid distorting the appearance of the beads.
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4 - Troubleshooting
4-1 Telephone Support
4-2 Error Screens
4-3 Identifying problems with Dispense
4-4 Troubleshooting Tables
4-5 Engineers' Log Extraction
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Note:
Please have a description of the fault, system settings and symptoms to hand when calling.
Region Telephone
Main Thermo Fisher Scientific Support 1 800 253 7837
Number, United States
Main Thermo Fisher Scientific Support +44 (0) 1928 562 520
Number, International
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Error Screens
ERROR 1
ERROR 2
ERROR 3
ERROR 4
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ERROR 5
ERROR 6
ERROR 7
ERROR 8
ERROR 9
ERROR 10
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ERROR 11
ERROR 12
ERROR 13
ERROR 14
ERROR 15
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ERROR 16
ERROR 17
ERROR 18
ERROR 19
ERROR 20
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ERROR 21
ERROR 22
ERROR 23
ERROR 24
ERROR 25
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ERROR 28
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When an error occurs, the flashing Error Number shown on the Main Menu
screen is the Operator Code for the error.
Pressing the flashing Error Number will display the Error Screen relevant to the
error encountered.
There will be a number in brackets in the top, right-hand corner of the screen -
this is the Engineers Code for the error.
The attached list cross-references each Operator Code with the Engineers Code.
The meaning of each Engineers Code is also given.
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The Mains fuses have Check the fuse in the Mains lead and
blown.
replace if necessary.
Other power faults. Check that the LEDs on the edge of the
main board are on, indicating that the
following voltages are present (top to
bottom):
15 V
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24 V
12 V
VCC (5 V)
3.3 V
Notes:
The 24 V indicator LED will only be lit
once the system has booted and is
running normally.
If the 15 V, 12 V, VCC or 3.3 V
indicator LEDs are not lit, replace the
main board (see Section 5-4-11-6).
Processor fault. If the 15 V, 12 V, VCC and 3.3 V
indicator LEDs are lit, but the 24 V
indicator LED fails to come on after
approximately 30 seconds, carry out the
following sequence:
Check that the interconnect board is
correctly mated with the processor
board and with the main board.
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Worn gripper jaws. Replace and set the gripper jaws (see
Section 3-4-8).
Excessive movement Check that coverslip transfer head is
of coverslip transfer
screwed in correctly (see Operator
head.
Guide Section 3-3).
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Select the image which most closely resembles the appearance of the coverslipped slide or
mountant bead.
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See Section 4-
4-6-12
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4-4-6-1
Do the following:
Check that the coverslip
transfer head hard-stop is
clean.
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4-4-6-2
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4-4-6-3
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4-4-6-4
Do the following:
Check that the coverslip
transfer head hard-stop is
clean.
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4-4-6-5
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hard-stop.
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4-4-6-6
If unsure about
contamination do the
following:
Reload mountant.
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4-4-6-7
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4-4-6-8
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4-4-6-9
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4-4-6-10
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cleaning station.
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4-4-6-11
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4-4-6-12
Flush the
system with
xylene
Replace
mountant with
fresh from an
unopened
bottle and
flush through
the system
Flush the
system with
xylene
Replace
mountant with
fresh from an
unopened
bottle and
flush through
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the system
Flush system
with Xylene
Replace
mountant with
fresh from an
unopened
bottle of
approved
mountant and
flush through
the system
(refer to the
Approved
Reagent List)
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Replace if
required
Replace motor
assembly if
required.
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Slow decay time. Check the Log file for decay time.
Should be approximately 3
seconds.
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suction cup.
Loss of vacuum on suction Clean suction cup and replace if
cup. necessary (see Operator Guide
Section 5-5).
Hopper not loaded Load hopper correctly (see Operator
correctly. Guide Section 2-14).
Incorrect size of coverslip. Use correct coverslip size for hopper.
Hopper end-stop set Inspect and adjust if necessary.
incorrectly.
Carriage hard-stop worn. Replace carriage.
Carriage vacuum ports Clean the vacuum ports (see
blocked. Operator Guide Section 5-6).
Coverslip dispense skirt not Refit coverslip dispense skirt.
fitted.
Coverslip dispense skirt Clean coverslip dispense skirt (see
needs cleaning. Operator Guide Section 5-9).
Coverslip dispense skirt Replace coverslip dispense skirt.
damaged.
Coverslip dispense skirt Clean coverslip dispense skirt and
unable to move freely in its
support plate.
slot.
Check for screws protruding
through plate or excess thread-
lock on sliding faces.
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8).
Coverslip transfer head Replace coverslip transfer head.
damaged.
Dispense head needle Set dispense head needle height
height set incorrectly. correctly (see Section 3-4-9).
Hard-stop for coverslip Check and adjust hard-stop (see
transfer head set Section 3-4-10).
incorrectly.
Basket eject bracket bent. Inspect basket eject mechanism (see
Section 3-2-5 and Section 3-3-11).
Excessive end float allows Check for end float on vertical lift
basket movement relative lead screw. Reach in and try to move
to motor and cause slides lift block up and down; any
to be in wrong position. movement greater than 0.05mm is
Note that it does not excessive. See section 5-4-25 for
create error message but setting end float.
may coincide with error
5[90], error 6[91] and
excessive variation in
logged lift top opto
position.
Basket open assembly not Inspect basket open assembly (see
pulling slider fully clear of Section 3-2-4 and Section 3-3-4).
slides.
Short gripper jaw breaks Check short gripper jaw stop cam
slide during vertical setting (see Section 3-4-13).
index.
Camera fault leading to Confirm using Log file data and
erratic sensing of basket replace if necessary (see Section 4-5
top and jaw 'y' positions. and Section 5-4-23).
Excessive play in long Replace gripper linear rail.
gripper linear rail leading
to incorrect sensing of jaw
'y' position.
Slide guide assembly Inspect slide guide and replace if
seized. necessary (see Section 3-3-5).
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approximately 25 mins
after a basket has been
processed.
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Note:Over-tightening of fittings
may cause thread to strip.
If fittings are in correctly but are
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Pipe not sealed onto Replace the pipe between the sensor
sensor. and the filter.
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Leak from Check that the end of the pneumatic line is cut
pneumatic line
square and is not damaged.
into fitting.
Ensure line is fully pushed home in fitting.
Fittings leaking. Check fittings are not cross threaded and are
fully tightened.
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Pipe not sealed Replace the pipe between the sensor and the filter.
onto sensor.
Filter leaking. Replace the filter between the sensor and the valve.
Pipe blocked by If pressure increases instantly then pipe is
mountant.
blocked with mountant.
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Flush system.
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Air in syringe
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De-gas.
Flush system.
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Replace as required.
Flush system.
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assembly.
Replace as required.
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No action required.
Flush system.
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The Event Log is a '.txt' file which can be read using various basic packages.
It takes the form of a 6000 event list in the following format.
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5-1 - Safety
The Safety and Warranty information must have been read and understood
before any work is carried out on this equipment.
Ensure that all Earth connections are correctly replaced.
Follow good laboratory practice at all times.
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1. Disconnect the power lead and switch the battery isolator switch off.
2. Remove the filter cover by sliding it to the left and then lifting it off.
3. Remove the charcoal filter.
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Step 2
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Step 3
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Step 4
1. Open the debris tray access door and remove the debris tray.
2. Unscrew the 2 fixing screws shown.
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Step 5
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Step 6
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Step 7
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Step 8
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Use the Production Services Inputs screen (see Section 2-3-1) to check the operation of
all door sensors, the filter switch and the fans.
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WARNING
UNLESS SPECIFICALLY STATED IN THE PROCEDURE, ENSURE
THAT THE INSTRUMENT IS SWITCHED OFF, DISCONNECTED
FROM THE MAINS SUPPLY AND HAS HAD THE BATTERY BACK-UP
ISOLATED!
FAILURE TO CARRY OUT THE ABOVE PROCEDURE CAN RESULT
IN FATAL ELECTRIC SHOCKS AND SERIOUS DAMAGE TO THE
INSTRUMENT!
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1. Turn the leadscrew using a flat-bladed screwdriver in the slot at the top, to raise
the lift block to an accessible height.
Note:
If the lift block is raised too far the front plate will move over and cover the fixing screws.
2. Remove the 3 fixing screws.
3. Remove the lift block assembly.
1. Position the lift block assembly so that it is butted-up to the datum face on the
linear rail adaptor plate.
2. Replace the fixing screws.
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Slide Pusher
1. Undo the retaining screw until it is clear of the slide pusher guide.
2. Remove the slide pusher by pulling it carefully off the slide pusher guide.
Note: Be careful not to lose the 2 springs.
1. Push the slide pusher onto the slide pusher guide ensuring that the springs are
located correctly in both sets of blind holes.
2. Push the slide pusher all the way onto the slide pusher guide so that the springs
are compressed.
3. Tighten the retaining screw so that it acts as a stop for the slide pusher.
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Opto Switches
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1. Screw tool T61627 to the top of the dispense head cleaning station actuator.
2. Move the actuator up until the tool touches the underside of the dispense arm.
3. Take control of the 'N_Pot' motor from Production Services (see Section 2-4).
4. Move the opto assembly upwards until the 'UP' opto is just activated, and then
lock the opto assembly in place.
5. Check the setting by using the minor increment arrows to move upwards - the
opto should not be seen - and downwards - the opto should be seen.
6. Replace the adaptor shaft and the dispense head cleaning station.
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Actuator Assembly
Note:
It is advisable to wind the linear actuator nut off the end of the shaft to gain access and tighten
the 3 flange fixing screws.
Replace the nut and push it into contact with the motor before tightening the bracket fixing
screws.
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1. Remove the dispense head cleaning station and the adaptor shaft from the top of
the actuator rod.
2. Remove the 2 mounting plate fixing screws located around the back of the vertical
plate.
3. Disconnect the electrical connector from the rear I/F PCB.
4. Remove the actuator assembly and mounting plate as a single unit.
5. Remove the 4 actuator fixing screws to release the actuator from the mounting
plate.
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1. Disconnect the actuator electrical connectors from the front I/F PCB.
2. Remove the electrical connector from the bottom of the solenoid body.
3. Remove the Nyloc nut from the top of the actuator and lift the bracket upwards
to clear the thread.
4. Unscrew the syringe from the manifold using the knurled section at the bottom.
5. Remove the 2 manifold fixing screws.
6. Remove the assembly.
7. Unscrew the 4 screws securing the actuator to the mountant solenoid assembly.
8. Remove the elbow bracket.
Note:
Ensure all wires are secured away from the moving parts and the syringe is fully screwed in to a
firm finger-tightness.
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1. Disconnect the mountant actuator electrical connector from the front I/F PCB.
2. Release the cable-ties.
3. Remove the Nyloc nut to separate the actuator from the bracket.
4. Remove the 4 fixing screws to release the actuator from the manifold.
Note:
Ensure all wires are neatly cable-tied away from the bracket.
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1. Disconnect the pneumatic pipes, taking note of their routing to facilitate re-
assembly.
2. Unscrew the 2 fixing screws shown for the assembly required.
3. Remove the required assembly.
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Step 1
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Step 2
1. Loosen the belt tension pulley using the 2 fixing screws on the back of the motor
mounting plate.
2. Remove the vacuum regulator fixings.
3. Remove the 3 Nyloc nuts which secure the motor mounting plate to the studs.
Note:
Do not lose the tensioning spring.
4. Pull the motor assembly and mounting plate off the studs.
5. Replace the motor assembly, pulleys or belts as required.
6. If refitting the motor assembly, ensure the wires are oriented as shown.
7. If refitting a pulley, set the height from the mounting plate as shown, and tighten
the clamp screw to 1.2Nm as per current methods.
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Step 3
1. Fit the timing belt over all the timing pulleys and under the tensioning pulley.
2. Replace the motor assembly and mounting plate onto the studs and secure with
the relevant fixings.
Note:
Do not tighten the pillar screws yet.
3. Orient the bottom pulley as described in the previous step to ensure the flag is
correctly positioned in relation to the opto switch.
4. From the front, fit the upper drive arm - do not tighten yet!
5. Re-insert the pin in the lower pulley shaft, then fit and secure the lower drive arm
(inc. main mounting block).
6. Move the lower drive arm to its TDC position and place a straightedge along the
vertical side face.
7. Align the upper and lower drive arms so that they are parallel, using the
straightedge as a guide.
8. Tighten the upper drive arm.
9. Using a square, as shown, ensure the main mounting block is perpendicular to
the vertical plate, then tighten the pillar screws.
10. Loosen the spring fixing nut to allow the tensioning spring to rotate.
Note:
If necessary, remove tension from the belt by loosening the 2 tensioning system fixing screws.
11. Remove the lower fixing screw and take off the spring washer - then replace the
fixing screw and tighten it.
12. Remove the home opto to prevent damaging it.
13. Back of the lower fixing screw by ¼ of a turn to allow the tensioning system to
slide but still remain well located.
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Step 4
1. Carefully stretch the tensioning spring so that the loop on the free end can be
hooked over the loosened upper fixing screw.
2. Rotate the dispense head mechanism to ensure that any belt slack is evenly
distributed.
3. With the tensioning spring in position, tighten the lower fixing screw.
4. Un-hook the tensioning spring from the upper fixing screw and return it to its
'parked' position - tighten the Nyloc to fix it in place.
5. Tighten the upper fixing screw.
6. Remove the lower fixing screw, replace the spring washer and then replace and
tighten the screw.
7. Re-check the drive arm alignment using a straightedge.
8. Replace the home opto.
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Identification of parts
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Check that the latest software version is installed (see Section 3-4-12).
Perform a 'Reset 7' (see Section 2-5-3).
Carry out ProdTst5, ProdTst6 and ProdTst7 (see Section 2-5-1).
Perform an 'Autoslip' (see Section 2-5-6).
Perform the Setup procedure (see Setup Instructions).
Carry out Performance Test 2.
Carry out Performance Test 1.
Ensure all settings are correct.
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5-4-11-3 - Battery
5-4-11-7 - Speaker
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1. Ensure the mains power and battery isolation switches are in their 'Off' positions.
2. Disconnect the mains power cable.
3. Remove all of the M4 SEMS which secure the electronics box cover to the
electronics box case.
4. Disconnect the earth lead from the cover.
5. Disconnect the PSU and speaker connectors from the PCB.
6. Disconnect the 3 wires (L, N and E) from the PSU.
7. Unscrew the 2 Nyloc nuts and lift the PSU bracket out of the case.
8. Remove the fixing screws which secure the PSU to the bracket.
9. Disconnect the remaining wires, taking note of their routing for reassembly later.
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5-4-11-3 - Battery
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Note:
Ensure the fan assembly is fitted with the cut-out oriented as shown, and with the air-flow
drawing air into the box.
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From the INPUTS screen (see Section 2-3-1) check the operation of the fan.
The frequency displayed should be > 80Hz.
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1. Remove the electronics box cover and PSU bracket (see Section 5-4-11-1).
2. Disconnect the battery lead from the PCB.
3. Pull the PCB off the mounting studs.
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Check that the latest software version is installed (see Section 3-4-12).
Perform a 'Reset 7' (see Section 2-5-3).
Carry out ProdTst5, ProdTst6 and ProdTst7 (see Section 2-5-1).
Perform an 'Autoslip' (see Section 2-5-6).
Perform the Setup procedure (see Setup Instructions).
Carry out Performance Test 2.
Carry out Performance Test 1.
Ensure all settings are correct.
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5-4-11-7 - Speaker
1. Remove the electronics box cover and PSU bracket (see Section 5-4-11-1).
2. Remove the 4 push-fit rivets to release the speaker assembly.
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1. Ensure the Mains power and battery isolation switches are in the 'Off' position.
2. Remove the electronics box cover (see Section 5-4-11-1).
3. Fuse kit A79210152 contains 3 x F12A, 250V fuses.
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Procedure 1:
Dispense Motor Assembly
1. Remove the touch-screen assembly (see Section 5-4-18 to remove the Display PCB,
then Section 5-4-20 to remove the touchscreen).
2. Loosen the 2 fixing screws which secure the pulley mounting block to release the
belt tension.
3. Remove the belt from the pulley on the dispense motor shaft.
4. Remove the 2 fixing screws which secure the motor assembly to the horizontal
plate.
5. Disconnect the electrical connector from the rear I/F PCB.
1. When fitting the motor pulley tighten the clamp screw to 1.2Nm.
2. Set the top face of the motor pulley to a height of 17mm from the mounting plate
as shown.
3. Tension the belt using a spring balance to apply 3kg of force to the pulley
mounting block whilst tightening the screws.
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Note:
Always apply the tensioning force as close to the mounting block centreline as possible.
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1. Remove the timing belt from the pulleys as described in Dispense Motor
Assembly.
2. Remove the 2 fixing screws to allow the timing belt clamp to be removed.
3. The timing belt should now be free to be removed.
Note:
Ensure the timing belt is tensioned as described in Dispense Motor Assembly.
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Procedure 2:
Dispense Motor Assembly
To remove the dispense motor assembly:
1. Remove the slip dispense opto cover and the display cable cover (see Section 5-4-
18).
2. Loosen the belt tensioner locking screw to release the belt tension.
3. Remove the motor cover.
4. Remove the belt from the pulley on the slip dispense motor shaft.
5. Remove the 2 fixing screws which secure the motor assembly to the horizontal
plate.
6. Disconnect the electrical connector from the rear I/F PCB.
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1. When fitting the motor pulley tighten the clamp screw to 1.2Nm.
2. Set the top face of the motor pulley to a height of 17mm from the mounting plate
as shown.
3. Tension the belt using a spring balance to apply 1.7-1.8kg of force to the belt
tensioning bracket whilst tightening the locking screw.
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Note:
Ensure all washers are in place at the ends of the linkage bars.
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1. Remove the 2 mounting plate fixing screws from above the horizontal plate.
2. Withdraw the leadscrew from the flanged lead-nut by sliding the actuator
assembly out in the direction shown.
3. Remove the 4 actuator fixing screws to release the mounting plate.
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Note:
Ensure all electrical connectors and pipework are correctly routed and that all the pipework is
fully pushed home.
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1. Remove the 3 casting fixing screws to allow the mountant bottle support casting
to be removed.
2. Disconnect the electrical connectors from the front I/F PCB and cut any cable ties
to free the wires.
3. Loosen the 2 fixing screws securing the motor bracket to the vertical plate to allow
the belt tension to be released.
4. Remove the belt from the pulley and lift the motor bracket off the fixing screws.
5. Remove the 4 fixing screws which attach the motor to the bracket.
Note:
Ensure all wires are neatly cable-tied in their original bundles.
Note:
When refitting the timing belt, tension it to 2kg using a spring balance.
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1. Remove the 4 pairs of fixing screws to allow the assembly to slip apart.
2. Pull the bearing blocks off the leadscrew at either end.
3. Undo the pulley clamp to allow the pulley to be removed.
4. The carriage assembly can be removed from the leadscrew by screwing it off the
end.
5. Release the push-fit rivets to remove the opto switch.
6. Remove the 2 proxy fixing screws to release the proximity switch.
Note:
When refitting the opto switch ensure the wires are routed as shown to leave clear access to the
leadscrew end slot.
Note:
When screwing the 2 halves of the rail back together, ensure the faces are properly aligned.
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1. Remove the countersunk screw, and subsequently the bearing and spacer.
2. Withdraw the pop-out pin and spring from the rail.
To refit the bearing and pop-out pin follow steps 1 and 2 in reverse.
Note:
Ensure the flat on the pop-out pin is on the correct side, and that the pin moves freely without
snagging.
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Carriage Assembly
1. Ensure all parts are free from burrs and move freely without snagging.
2. Ensure the chamfers on the latch pins are facing the correct way.
3. Use Loctite 222 on the countersunk fixing screws.
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Note:
If only the retractable latch requires removal, this can be done without disassembling the load
rail - wind the carriage as far towards the pop-out pin as possible and the latch will protrude
from the load rail.
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To refit the display PCB and the proximity switch follow the above steps
in reverse.
Note:
Ensure the fold of the ribbon cable stays within the recess on the touchscreen.
Note:
If changing the proximity switch, use a piece of Armaflex tape to hold the new switch in place.
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Use the INPUTS screen to check the operation of the doors (see Section 2-3-1).
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Note:
The leaf spring can be replaced with the slide guide in-situ; remove the lower main guide fixing
screw and pull the leaf spring out.
1. Remove the various sets of fixings shown to enable the assembly to be separated.
1. Ensure the mating edges / faces of all components are fully butted-up to one
another.
2. When fitting the slide guide assembly to the horizontal plate, push the assembly
so that it butts-up to the linear guide and then check that it is square to the plate
using an engineers square.
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Note: Ensure that the alignment face on the arm is butted-up to the linear guide carriages.
Note: Ensure that no debris gets stuck under the support arm during fitting as this could make
it impossible to set the end stop correctly.
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1. Remove the 4 fixing screws shown and the 2 push-fit rivet (not shown) to allow
the 2 camera covers to be removed.
2. Disconnect the ribbon cable from the camera board.
3. Release the 2 fixings which secure the camera mounting block to the horizontal
plate.
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1. Ensure the top edge of the motor pulley is 17mm from the mounting plate face as
shown. The correct torque for the pulley clamp is 1.2Nm.
2. Tension the belt using a spring balance to apply 2kg of force to the assembly
whilst tightening the screws.
Note:
Ensure the tensioning force is applied as close to the centreline of the fixing screws as possible.
And Then...
Carry out Performance Test 2
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1. Remove the cap-head screw which fixes the drive transfer plate to the lift block.
2. Remove the timing belt (see Section 5-4-24).
3. Remove the clamp and pulley from the bottom of the leadscrew.
4. Remove the bearing block from the top of the leadscrew.
5. The leadscrew and drive transfer plate can now be removed.
1. Position the new assembly so that the drive transfer plate can be loosely fixed to
the lift block.
2. Move the lift block to the bottom of its travel then tighten the screw to secure the
drive transfer plate to the lift block.
Note:
Use Loctite 222 on the screw thread.
3. Raise the lift block to the top of its travel and fit the bearing block.
4. Refit the pulley and clamp to the bottom of the leadscrew. The correct torque for
the pulley clamp is 1.2Nm.
Note:
Ensure the thrust washer is in place as shown.
5. Tension the timing belt (see Section 5-4-24).
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Check the alignment of the leadscrew by moving the lift block from top to
bottom using a 0.1Nm torque.
Note:
Timing belt must be removed or lift motor must be disconnected from front I/F PCB to carry
out torque test.
Initialise the instrument.
Carry out Performance Test 2.
Carry out Performance Test 1.
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Note:
The gripper jaws can be a tight fit on the dowel pins and also tend to get stuck with mountant.
If the jaws are difficult to remove, then use the slots shown below to pry a gripper jaw off the
support arm.
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1. When replacing the long gripper inspect the paint on the new gripper arm to
ensure that it is undamaged.
2. Replacing the gripper arm is the reverse of removing the gripper arm.
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Insert the floppy disk containing the software update into the disk drive.
Press 'Software Update'.
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If the software update is successful, the ClearVue will power-down and then
reboot.
After power-up and initialisation, navigate back to the Disc screen and check that
the software and FPGA version numbers have been updated.
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6 - Service Schedule
ENSURE ALL CLEARVUE SERVICE BULLETINS HAVE BEEN
RECEIVED.
NOTE MODIFICATION STRIKE LABEL - INCLUDE ANY
OUTSTANDING UPDATES IN THE SERVICE
TEST INSTRUMENT FOR EARTH CONTINUITY AND INSULATION
RESISTANCE!
IF THE INSTRUMENT FAILS, DO NOT PROCEED WITH THE
SERVICE UNTIL THE CAUSE HAS BEEN ESTABLISHED AND
RECTIFIED!
E - Torques.
F - Belt tensions.
G - Replace.
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Note:
When lubricating linear rails use a LIGHT MICROTOME OIL - NOT GREASE!
1. Load rail, unload rail and lift block (clean basket wear debris).
2. Lubricate vertical lift rail.
3. Lubricate basket unload rail (2 carriages).
4. Lubricate load rail spring plungers (4 off).
5. Load rail spring plungers (ensure free movement).
6. Lift block spring plunger (ensure baskets retained positively).
7. Lift block (ensure no loose play in block).
8. Basket open (ensure sprung loaded arm moves freely).
9. Lubricate basket open rail.
10. Lubricate basket open spring loaded arm.
11. Slide guide block (moving freely, not damaged).
12. Lubricate slide guide block rail.
13. Slide alignment spring (replace if damaged).
14. Basket close arm (moving freely).
15. Lubricate basket close arm rail.
16. Coverslip transfer head (no excessive play).
17. Check for wear and reset the position of the coverslip dispense end stop.
18. Pipework (replace any chaffed / kinked pipes).
19. Backlash on syringe drive (tighten knurled screws if required).
20. Fans (ensure turning and quiet).
21. Baskets (where possible check for wear/breakages).
22. Basket sliders (ensure clean runners and smooth movement).
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23. Basket sliders (ensure white sliders are clean and not heavily stained).
24. Xylene tray.
25. Waste tray.
26. Output rail drip tray / camera cover.
27. Coverslip dispense carriage and its vacuum chamber.
28. Lubricate coverslip dispense rail.
29. Dispense head cleaning station.
30. Dispense head.
31. Camera lens.
32. Gripper eccentric.
33. Lubricate gripper rail (2 carriages).
34. Gripper stripper mechanism.
35. Coverslip transfer head end stop.
36. Coverslip transfer head: check for smooth movement of the sprung blocks.
37. Slip dispense opto’s
38. Carry out Daily and Weekly cleaning tasks as per Section 2-1 and Section 2-2 of
the Operator Guide.
I - Test.
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K - Take event log and store results on floppy disc with note of serial
number and date - Return to your local service representative.
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7 - Spares List
General
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Couplings
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Covers
Belts
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Linear Rails
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8 - Technical Drawings
8-1 Electrical Drawings
8-2 Pneumatic Drawings
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Always ensure you are working from the current issue of the Pneumatics Schematic.
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Appendices
Appendix A - Service Fault Codes
Appendix B - Parameters
Appendix C - Understanding Log Files
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A2 - Cause Codes
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Appendix B - Parameters
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Summary.
This is a quick reference sheet to highlight areas in the log file that need to be scrutinised,
this could be a sheets that a service engineer could print and file for reference.
Errors.
List of every error in the log file (shows all slips errors & not just the ones the user see’s)
Prep decay data in easy to read chart format - see below for further explanation on prep
decay charts.
Warnings.
Prep decay.
Data for every decay time logged. Limits as per the decay chart see below for further
information.
Opto chart.
The absolute values are not critical (typically between 200 and 800, acceptable limits are
100 to 900), however an increase in the optical sensor reading indicates a reduction in
the signal strength typically caused by glass dust on the sensors. The sensors are housed
just in front of the coverslip dispense skirt and can be cleaned from below with a soft
brush. Note, when no coverslip is present the value is typically 1300-1400 however it is
not expected that these values will be see on the logs.
Slip optos.
Slip dispense.
Shows logged values for slips dispense Quad A, B, C & D values plus error code.
Includes a quick reference table with error quantities shown; see below for further
information on slip dispense values.
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Voltages.
Values are logged immediately after switch on and every hour thereafter. Values are also
logged when a power fail is detected.
The voltages readings are in units of approximately 10mV. 2400 ≈ 24V.
V PSU is the voltage detected from the PSU. This will typically be around 24V. Values
of 18-20V are typical at power fail (when the unit is switched off).
V BATT1 is one of the backup batteries. This will typically be in the range 12-15V
depending on state of charge. Lower values can be expected during periods when the unit
is running on batteries.
V BATT2 is the second of the backup batteries. When the unit is running on batteries
this will show the total voltage of the two batteries, typically 26-28V immediately after a
mains power fail.
The OC and STL signals indicate the charger status.
Hz ELEC is the speed of the electronics box fan. Typically 95-115Hz. This fan should
run continuously.
Hz EXTR is the speed of the extract fan. This is 0 when the unit is first switched on or
when the filter is not fitted. The fan has 2 running speeds, 70-90Hz is the low speed,
115-125Hz is the high speed.
°C Elec is the temperature sensor on the battery charger board indicating the
approximate internal temperature of the electronics box.
mp_load.
lift opto.
Logs when lift top opto seen during eject sequence, we are interested in how much the
value varies. Based on the random check of 12 of the last 30 units the value varies
between 0 & 11 quads.
slide width.
Actual width of slide = [logged width of jaws] + 5.6 - (2 x [slide thickness]). When you
take into account the tolerances on slides and the jaws the logged values should be in the
range 18.1mm - 23.4mm. Software rejects widths below 16mm and above 23.7mm.
Tab is not all that useful as we are not really interested in the variation but could be used
to see if slides are close to maximum width. May be more useful if plotted on a chart
since it would show when values are tending to trend upwards due to the gripper return
plate getting tacky.
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basket load.
Shows logged values for stall quad, max & pos, load quad & pos & basket open quads.
These are again values where we are concerned with variation rather than absolute limits.
There are 3 logged values, the first value is the final stall
quad, the second is the further the load rail pusher has jumped back when stalling on the
basket. This value would normally be just less than the first logged quad value. A large
difference in both may indicate problems with load rail belt tensions.
jaw_y
We are interested in the variation of jaw x & y. Jaw y should not vary by more than ± 1
pixel so the difference in variation values should be <=2.
Note:
Software version 1.78 issue 8 centres the jaw_x and jaw_y to fixed positions.
Jaw_x should be 225 and jaw_y should be 65.
Note:
There will be log events of the original jaw_x and y positions before adjustment to compare with
previous values taken.
basket top.
As with jaw y we are looking for consistency across top 1 & 2 for each of the basket
types.
On most instruments you will see:
Both values are consistent.
One value is consistent and the other value varies by 1 pixel.
Both values vary by 1 pixel.
In any of these cases the unit will run fine.
There have been some units where one of the values varies by 2 pixels and the other is
either consistent or varies by 1 pixel. The majority of the time the unit will still handle
slides without issues, however if the jaw y value also varies by the maximum amount or if
the jaws are at the maximum allowable runout you will start to see an increase in error
6(91)’s. A unit may also give errors if the basket runout is too high, this is not checked
and can not be adjusted.
If either of the basket top values varies by more than 2 pixels or both vary by 2 pixels the
unit will most likely start to give errors. However this is not always the case since if
everything else nominal the unit can carry on functioning OK. So far the only units that
have exhibited this much variation have also showed variation in jaw y values and it has
been indicative of Camera problems.
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basket times.
Shows all the logged basket processing times and can be useful for a quick check it the
customer has not been clearing errors in a timely fashion, especially if there are
complaints about drying. - see below for further information about basket times.
start of basket.
Shows the profile version, the set and used mountant levels as well as slide threshold
(P72). See below for further information about start of basket.
software version.
sorted data.
Just used to create the other tabs but can be useful if additional data extraction is
required.
original data.
Original data untouched apart from separating date, time & log entry into separate
columns.
Note:
If you sort this tab you may not be able to put it back in the correct order as many log entries
have identical time stamps.
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Further Information
prep_decay_chart
This is likely to be caused by suction cup contamination. Clean the suction cup and
check for broken slips or excessive overspill. Use the Prep Decay tab to find the
actual value.
Check for leaks; contaminated suction cup (broken glass or mountant), damaged
suction cup. Check pipe is pushed into the fittings firmly.
This is the limit for good coverslip quality. Below this decay time there is a high risk
of the slip slapping down onto the slide causing multiple bubbles on each slide. Any
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machine running below 1.2 seconds should be checked by a service engineer and
fixed.
Decay time with a general trend upwards (more than 2 seconds over
400 slides).
This could be related to temperature and how the restrictor that controls the release
of vacuum has expanded and restricted the flow. Monitor the situation. Check that
the machine has a decay reservoir fitted and fit where required. Reset the decay time
to 3 seconds.
This will slow the machine down, but will not affect the quality of the prep. The
priority of the customer should be considered: Prep quality V’s speed of the
machine. Errors are reported if the slip has not been released after 13 seconds.
prep_decay
mp_load
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load
cycle at
quad:
Date Time Quad reached Position
This has been used as a development tool along with 'dispense end' values to determine
how much mountant has been dispensed.
The Quad value is from the encoder and is the position actually reached. The position
value is the position demanded of the motor and where it is aiming for.
A difference of more than 100 between quad and position shows there has been some
stall on the motor.
The syringe should refill to ±0.5mm each time (approx 165 quads)
333 quads = 1mm of syringe movement
basket_times
start_of_basket
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slip_dispense
This page shows you how many slips have been dispensed, the engineering error code
with how many errors, plus the % of errors in that category.
If double slips are reported find out if there are actually 2 slips dispensed on the carriage
together. If so check the end stop height, if not, use the Autoslip to reset the thresholds
for no slip and double slips.
If length errors are reported check there is sufficient vacuum on the carriage.
The following text is the cycle of one basket through ClearVue. This is the pattern you
should see when looking at raw log files. Each line has a description of what is
happening.
Basket detection has started.
09/11/2008 11:27:40 GOOD INPUT STALL DETECTED : quad:13554 Min
Quad seen:13554 Pos:13623
Basket has been detected by load rail, 1 basket found.
09/11/2008 11:27:40 Input Rail basket count = 01
Basket has been loaded to lift
09/11/2008 11:27:42 Basket loaded to lift success @ quad = 14169 and position =
14213
Camera jaw_x and jaw_y are re-tested to ensure no change in image has been seen.
Ignore NEW POS 500 value.
09/11/2008 11:27:43 camera JAWY_Y re-tested from below, old pos=065 new
pos=500
This line shows the prep head has checked the pin width on the prep transfer head
and has detected a normal (around 40 ) prep head in the END position.
09/11/2008 11:27:43 Prep head pin width:0040
This line is showing the calculated position of the jaw using a different method,
values should closely resemble that of the above jaw_x and jaw_y by +/1 1 pixel.
09/11/2008 11:27:43 AUX CAM INIT NEW: jaw_x, 226, jaw_y, 064 OLD:
jaw_x, 225, jaw_y, 065 USED:0
This line showes a check the camera does on the image, it takes one image and
examines the image for HALO effects which are often caused by dirty or out of
focus lenses. Clean values greater than 6/7 should be investigated. Typical values are
2/3
09/11/2008 11:27:43 Camera Lens QC Check : High thresh : 037, Low Thresh
:039, CLEAN VALUE :002
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This shows the quad of the basket open slider when fully opened.
09/11/2008 11:27:45 Basket slider opened @ 11043
Basket processing is about to begin. OFFSET target is parameter 92 value. Jaw at is
current jaw_y value.
09/11/2008 11:27:47 Start of basket. OFFSET TARGET=004 JAW_AT:065
Detection of basket top has started, first picture has been taken and analysed as
shown. It can be seen that both basket top values are the same value and equal to
white strip bot. this indicates the basket is not in view of the camera and therefore is
a smaller 20 slide basket. White strip bot value is the lowest part of the white strip
the camera can see. Notice BIG,SMALL value is 1 which indicates the lift is at BIG
basket position.
09/11/2008 11:27:47 (BIG BASKET CODE) Basket top 1,00085, Basket top
2,00085, White Strip bot,00085, BIG(1)SMALL(0),1 At lift quad, 09610
Second basket top position, now at SMALL basket position. You can see the basket
top values are 71 and 69. two pictures are taken for basket top, one is 11 quads
higher than the other. This gives the values of 71 and 69 and are different to white
strip bottom - this means a valid basket top has been found. Notice BIG,SMALL
VALUE is 0 indicating lift at SMALL position.
09/11/2008 11:27:49 (BIG BASKET CODE) Basket top 1,00071, Basket top
2,00069, White Strip bot,00085, BIG(1)SMALL(0),0 At lift quad, 09059
The lift and camera have worked out from all the info above, the correct position to
move to for processing the basket. This is known as the final 1st slide position.
09/11/2008 11:27:49 (BIG BASKET CODE) Final 1st Slide Position,08723, Total
Slides to Process,00020, Basket top1,071, top2,069
This values shows the colour of the basket slider, low values indicate BLACK sliders,
high values for white.
09/11/2008 11:27:49 Basket slider white percent : 001
First slide position has been reached. Quad and target values should be within 1
quad of each other.
09/11/2008 11:27:49 Lift @ 1st slide position, quad:08723, pos:08723,
Target:08723
A quick reminder of the profile version and mountant settings used on this basket.
09/11/2008 11:27:49 start of basket processing - Profile V:87 @ setting :05
Black:05 White:12 Slide Thresh:095
This chunk of events shows a complete cycle of removing the slide and performing
the prep, these events should be repeated 20 times for small baskets.
Pos values indicate what is visible to the camera, pos0 is current slide slot, 1 is next
and 2 is after next slot. Values of 15 will detect a slide as present. Decreasing the
camera slide threshold parameter will increase these numbers but will more likely
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cause ClearVue to grab slides not present. Decay time is another important number
here, which gives indication to machine performance within the pnumatic system.
09/11/2008 11:27:50 pos_0:050 pos_1:043 pos_2:090 @ quad:08723 Slide No:01
09/11/2008 11:27:50 Gripper detected slide ok, final pos adjust = :-14 Quad
1:56582 Quad 2:57221 Width of Jaws: 21.94 mm
09/11/2008 11:27:52 DISPENSED SLIP PROPERTIES -> qa:10194 qb:10194
qc:10468 qd:10468 er_no:00
09/11/2008 11:27:52 Good slip, optics at, 00292, 00230
09/11/2008 11:27:54 dispense end @ Qd:12085 pos:12087 Suckback end @
Qd:12032 pos:12029 with timer:00042
09/11/2008 11:28:00 Prep Decay Time - 03.42
..
..
..
..
..
Finally we reach basket end, which shows the total time to process the basket.
09/11/2008 11:31:23 Basket processing finished Total time, 03:30
This quad value is that of the lift when the flag passed the upper lift opto. Variation
in this value should be minimal.
09/11/2008 11:31:30 Lift TOP opto seen->not_seen at:03468
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Warranty Statement
We at Thermo Fisher Scientific are proud of our quality, reliability and of our after-sales
service. We continuously strive to improve our service to our customers.
Please ask your distributor or Thermo Fisher representative about Service Contracts
which can keep your purchase in peak condition for many years to come.
Warranty provisions necessarily vary to comply with differences in national and regional
legislation. Specific details can be found in the delivery documentation or from your
dealer or representative.
Please note that your warranty may be invalidated if:
The instrument is modified in any way.
Accessories and reagents which have not been approved by Thermo Fisher
Scientific are used
531
Product Return Safety Declaration
533
Index
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remove from vertical lift .................50 output pusher and upper slide
retention ..................................250
slide retainer colour ........................69
output rail....................................251
troubleshoot - jamming................349
pneumatic circuit .........................256
unload............................................42
slide grippers ................................244
Basket open assembly
slide guide....................................243
description ...................................163
vertical lift and lift block ..............241
inspecting.....................................242
C
replace..........................................396
Camera
Basket recognition..............................69
camera board replacement............483
Battery
clean ............................................117
description ...................................168
description ...................................163
remove .........................................434
536
Index
537
ClearVue Service Manual (A79210101_04)
538
Index
539
ClearVue Service Manual (A79210101_04)
inspecting.....................................246 P
Mountant dispense needle Parameters .......................................519
540
Index
PSU Sensors.............................................168
541
ClearVue Service Manual (A79210101_04)
542
Index
W X
Weekly tasks ......................................13 Xylene tray.........................................19
Xylene tray seal ..................................89
543
544