Assessment for Zika Virus Infection

in Nonpregnant Individuals

■ Nonpregnant individuals with possible Zika virus exposure

■ Evaluate for signs and symptoms of Zika virus disease
(eg. fever, conjunctivitis, rash, arthralgia)
■ http://wwwnc.cdc.gov/travel/page/zika-travel-information

symptomAtic AsymptomAtic

<2 weeks after symptom onset 2-12 weeks after symptom onset >12 weeks after symptom onset Testing not recommended

Zika virus rRT-PCR (serum and urine) MZIKV / Zika Virus IgM Antibody
■ RZIKS / Zika Virus, PCR, Molecular Detection, Serum Capture MAC-ELISA, Serum
■ RZIKU / Zika Virus, PCR, Molecular Detection, Random, Urine

Positive Zika virus rRT-PCR Negative Zika virus rRTPCR Result: Presumptive Result: Other Flavivirus Zika virus IgM Negative
(serum or urine): Recent (serum and urine): no recent Zika Positive or Positive indicates antibodies
Zika virus infection infection with Zika virus Possible Zika Positive were detected to a Flavivirus,
but not to Zika virus

Consider testing Collect follow-up Confirmation testing by a plaque Consider follow-up testing for dengue virus and/or West ■ No recent Zika virus infection
for co-circulating serum specimen 2-12 reduction neutralization test (PRNT) Nile virus ■ No further testing necessary
viruses (eg, Dengue weeks postexposure is required (available through CDC ■ DENGM / Dengue Virus Antibody, IgG and IgM, Serum

or chikungunya) or return from travel and select public health laboratories) ■ DENVP / Dengue Virus Antibody/Antigen Panel, Serum

■ WNS / West Nile Virus Antibody, IgG and IgM, Serum

Testing of asymptomatic Zika virus-exposed partners

of pregnant women is not recommended
• Barrier protection or abstaining from sex during pregnancy is recommended
• For individuals considering conception postexposure, visit www.cdc.gov/zika/
prevention/protect-yourself-during-sex.html for up-to-date
CDC recommendations

Serologic and molecular testing is recommended for infants suspected to have

contracted Zika virus in utero.

Adapted from Oduyebo T, Igbinosa I, Petersen EE, et al: Update: Interim Guidance for Health Care Providers Caring for Pregnant
Women with Possible Zika Virus Exposure – United States, July 2016.MMWR Morb Mortal Wkly Rep 2016;65:739-744

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