Manual Servicio cm200

Wiener lab.
CM 200
Automated Clinical Chemistry Analyzer
SERVICE MANUAL
WARNINGS
This manual applies to those instruments delivered

from factory with software version 3.11 and later.
1) Only connect instrument to a line complying with local or national rules and
specifications.
2) Never use instrument for a purpose other than specified by manufacturer.
(For purpose description, See Chapter 1).
3) Never turn instrument on without waiting at least 20 seconds after turning it off.
4) Do not connect monitors, printers or unauthorized cables in RS232 outputs of
instrument.
5) Do not open rear or left cover of instrument before reading specific
servicing situations described along the present manual.
6) To change lamps and other elements, follow directions included in the present
manual.
7) The use of most screen savers can affect communication between PC and
Analyzer. Use only “Windows Curves and colors” at its minimum speed, if a
screen saver must be utilized.
8) Maintain the cover down during operation to avoid danger from moving parts and
to improve performance of the instrument.
For technical assistance, please contact local representative or directly to

factory
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Safety symbols used in instrument:
Warning: Before using read instructions in

Manual
Hazardous Voltage
Ground connection
Representative for CE:

MT Promedt Consulting
GmbH
Altenhofstr. 80
D-66386 St. Ingbert / Germany
Tel.: +49 6894 - 58 10 20
Fax: +49 6894 - 58 10 21
www.mt-procons.com
Warranty is subject to the following conditions:
Trained personnel must perform installation.
Installation Checklist and Test Report must be sent to

manufacturer.
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WARNINGS on Instrument and Laboratory practices
1) Perform daily, weekly and quarterly maintenance procedures, as specified in user

manual. Keep records on actions and dates.
2) Perform instrument tests as indicated in user manual. Any departure from
specifications should be consulted with the Service Department. Keep records on
tests and calibrations of instrument. Compare data with previous information.
3) Perform all maintenance repairs and replacements as required by manufacturer.
Elements such as drying block and tubing must be inspected daily.
4) The use of standards in every run. Factor values can be used instead of standards if:
a) Reagent belongs to the same lot for which factor was determined.
b) Standard absorbance did not vary more than ¼ of the allowed method
variation in the last few readings.
c) Instrument did not suffer a major repair (change of filters, lamp or
photometer).and photometer) since the last calibration.
5) To ensure adequate quality control, normal and abnormal control with assayed values
should be run as unknown samples.
a) At least every eight hours
b) When a new container of reagent is used
c) After preventative maintenance is performed, or a critical component is
replaced.
6) Control results are considered valid if:
a) Control values fall within the specified range.
b) Results for controls run at the beginning and end differ by an acceptable level
of variation. An acceptable level of variation is criteria determined by user, or
control manufacturer.
7) Read all warning messages at the end of the run. Results can be totally or partially
accepted or rejected if:
a) Reagent initial absorbance values fall within specified range.
b) Energy is within range.
c) There are non-stopping instrument errors.
8) Open the error file and check for repetitive mechanical errors. If errors in
Sample/Reagent tray or Reaction tray repeatedly occur, results must be under
suspect and eventually discarded.
9) Immediately after the run check if cuvettes are dry. If not, results of previous run are
under suspicion and should be carefully controlled and/or repeated.
10) Whenever a new reagent is introduced in the system cross-contamination must be
studied. The study should be performed by using the same reaction cuvettes for both
suspected reagents, in the interfering order: first the interfering, next the interfered
reagent. Study should consist of running precision tests on new reagent alone and in
contamination condition with other reagents. Acceptance criteria must meet normal
laboratory practice.
WARNING: Instrument provided with cuvette washer

must be used at all times with cuvette retainer cover,
otherwise cuvettes might be pulled out by washer unit.
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INDEX
1 DESCRIPTION.....................................................................................................................12
1.1 OVERVIEW...................................................................................................................12
1.2 OPERATING FEATURES........................................................................................................14
1.3 TECHNICAL SPECIFICATIONS................................................................................................16
1.4 INSTALLATION.....................................................................................................................20
2 ELECTRONICS...................................................................................................................21
2.1 AUTOANALYZER BOARDS:....................................................................................................21
2.2 GENERAL......................................................................................................................22
2.3 FUNCTIONAL DIAGRAM..........................................................................................22
2.4 COMMUNICATION PROTOCOLS...........................................................................23
2.4.1 Communication protocol between COMU and DILUTER.......................................23
2.4.2 Communication protocol between COMU and the PC............................................24
2.5 COMMANDS FROM PC TO THE COMU................................................................25
2.5.1 Probe arm robot.......................................................................................................25
2.5.2 Diluter ......................................................................................................................26
2.5.3 Photometer 990........................................................................................................26
2.5.4 Reaction Tray...........................................................................................................26
2.5.5 Sample / Reagents Tray............................................................................................27
2.5.6 Peristaltic pump........................................................................................................27
2.6 DESCRIPTION OF THE MESSAGES.......................................................................27
2.7 COMMUNICATION PROTOCOL BETWEEN COMU AND CARRU....................29
2.8 DESCRIPTION OF SERIAL PORT COMMANDS..................................................30
2.9 MULTIPLE COMMANDS, GENERAL.....................................................................34
2.9.1 Description of multiple commands...........................................................................36
2.10 INTEGRATION METHOD.......................................................................................44
2.10.1 General...................................................................................................................44
2.10.2 Carru......................................................................................................................44
2.10.3 Photometer (standard command)...........................................................................45
2.10.4 Photometer (multiple command):...........................................................................46
2.10.5 Diluter.....................................................................................................................46
2.10.6 Multiple commands:...............................................................................................46
2.11 DESCRIPTION OF STATUS BYTES.......................................................................46
2.12 DESCRIPTION OF THE STATUS BITS OF ISE MODULE AND BAR CODE
READER...............................................................................................................................48
2.13 NOTES ON THE DETECTORS................................................................................48
2.13.1 Collision detector...................................................................................................48
2.13.2 Capacitive level detection.......................................................................................48
2.13.3 Uninterrupted Voltage supply (Ups)......................................................................49
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2.14 STATUS ORDERS.................................................................................................................49
2.14.1 Photometer.............................................................................................................49
2.14.2 Carru......................................................................................................................50
2.14.3 Diluter.....................................................................................................................50
2.15 DIGITAL TEMPERATURE CONTROLLERS................................................................................50
2.16 INTERFERENCE FILTERS......................................................................................................51
2.17 CALIBRATION AND TEST PROGRAMS.....................................................................................52
2.17.1 Auxiliary board M1401-P151.................................................................................52
2.17.2 Comu test program ...............................................................................................53
2.17.2.1 Comu commands menu....................................................................................53
2.17.2.2 Comu normal mode..........................................................................................56
2.17.3 Test programs for carru.........................................................................................57
2.17.3.1 Parameter set mode..........................................................................................57
2.17.3.2 Sensor control mode........................................................................................59
2.17.3.3 Command simulation mode ............................................................................60
2.17.3.4 Automated sequence test mode........................................................................64
2.17.3.5 Carrusel in normal mode..................................................................................65
2.17.3.6 Carru test menu tree.........................................................................................65
2.18 CALIBRATION AND ELECTRONIC CONTROL................................................70
2.19 CPU BOARD (M230X-P213). ....................................................................................70
2.20 PREAMPLIFIERS (M85X-P160)..............................................................................70
2.20.1 Zero adjust..............................................................................................................70
2.21 PROBE ARM PCB (M230X-P222). .................................................................................70
2.22 REACTION TRAY HEATER (M230X-P216)........................................................................71
2.23 PUMP CONTROLLER (M230X-P216)....................................................................71
2.24 VERTICAL CONTROLLER (M230X-P216)...........................................................71
2.25 HORIZONTAL CONTROLLER (M230X-P216)...................................................72
2.26 SAMPEL/REAGENT TRAY CONTROLLER (M230X-P216)..............................72
2.27 REACTION TRAY CONTROLLER (M230X-P216)..............................................73
2.28 AUXILIARY BOARD (M1401-P151) TO COMU CPU..........................................74
2.29 PHOTOMETER CONTROLLER (M230X-P219)..................................................75
2.30 DILUTER CONFIGURATION....................................................................................................75
2.31 +5VDC POWER SUPPLY....................................................................................................75
2.32 INGRESO DE LAS POSICIONES HORIZONTALES........................................................................75
2.33 PROBE VERTICAL MOVEMENT TEST...........................................................................75
2.34 FILTER WHEEL OPERATION TEST.........................................................................................76
3 MECHANICAL, OPTICAL AND HYDRAULICAL SETUP.........................................78
4 FILES AND DATABASES INCLUDED IN THE SYSTEM............................................81

4.1 FILES.................................................................................................................................81
4.2 DATABASES.........................................................................................................................81
5 MAINTENANCE..................................................................................................................83
5.1 SELECTING WORKING TEMPERATURE.....................................................................................83
5.2 REPLACEMENT AND CONTROL OF WASH SOLUTION..................................................................83
5.3 PUMP TUBING AND SYRINGE REPLACEMENT. ..........................................................................83
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5.4 LAMP REPLACEMENT...........................................................................................................84
5.5 SAMPLE PROBE CARE...........................................................................................................85
5.5.1 Calibration procedure for probe arm (Only if required).........................................85
5.6 HYDRAULIC CIRCUIT CONTROL AND MAINTENANCE..................................................................85
5.7 CUVETTE WASHER MAINTENANCE..........................................................................................86
Diagnostic.........................................................................................................................86
5.7.1 Volume adjust...........................................................................................................87
5.7.2 Drying block replacement........................................................................................87
5.8 PHOTOMETER AND FILTER CLEANING.....................................................................................87
5.9 DETECTOR LENS CLEANING...................................................................................................87
5.10 PREVENTIVE MAINTENANCE PROGRAM...............................................................................88
5.10.1 Recommended daily care........................................................................................88
5.10.2 Inspection and cleaning of probe...........................................................................89
5.10.3 Weekly Care Recommendations.............................................................................89
5.10.4 Quarterly Maintenance Recommendations............................................................90
5.10.5 Maintenance on Demand........................................................................................90
6 TROUBLESHOOTING ......................................................................................................92
6.1 OPERATION MALFUNCTION WITH WARNING.............................................................................92
6.2 VISIBLE FAULTS .................................................................................................................93
6.2.1 Drop formation on probe tip after dispensing..........................................................93
6.2.2 Drop formation after wash cycle..............................................................................94
6.2.3 Abnormal noises.......................................................................................................94
6.2.4 Inaccurate Temperature readings............................................................................94
6.2.5 Automatic cuvette washer malfunctioning................................................................94
6.3 INCONSISTENT RESULTS........................................................................................................94
6.3.1 All Methods...............................................................................................................95
6.3.2 Colorimetric methods (one or more)........................................................................95
6.3.3 Symptom: Low linear range.....................................................................................96
6.3.4 Fast kinetics..............................................................................................................96
6.3.5 2-point kinetics.........................................................................................................98
6.3.6 Inconsistent values in automatic repetition or dilution............................................99
6.3.7 Coagulation..............................................................................................................99
6.4 MESSAGES AND WARNINGS................................................................................................101
6.4.1 Messages while not operating instrument..............................................................101
6.4.2 Run-time errors and messages...............................................................................103
7 VALIDATION PROGRAM FOR PARAMETER TESTING.......................................109
7.1 REQUIRED ELEMENTS.........................................................................................................110
7.2 DESCRIPTION OF TESTS......................................................................................................110
7.3 AUTOMATED VALIDATION TESTING....................................................................................110
8 COMMON SERVICING SITUATIONS..........................................................................111
8.1 CALIBRATION INTERPRETATION...........................................................................................111
8.2 MESSAGES RELATED TO ENERGY.........................................................................................111
8.3 VERTICAL AND HORIZONTAL ERRORS TOGETHER.................................................................112
8.4 PROBE STOPS BEFORE REACHING OR DOES NOT STOP AT ALL..................................................112
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8.5 PROBLEMS IN HYDRAULIC SYSTEM.......................................................................................112
8.6 CTS TIMEOUT MESSAGE...................................................................................................112
8.7 MESSAGES RELATED TO MECHANICAL PROBLEMS..................................................................112
8.8 UNDEFINED MESSAGES RELATED TO DATA BASES AND/OR MEMORY........................................113
8.9 CORRUPT INDEXES.............................................................................................................113
8.10 ALL READINGS OF SAMPLE AND OR REFERENCE CHANNEL ARE ZERO.....................................113
8.11 POOR DRYING ACTION; BLOCK DIRTY; BLOCK BREAKS EASILY.......115
9 ILLUSTRATIONS AND SCHEMATICS.......................................................................116
2.12 ISE Module is not available in this model
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1 DESCRIPTION
Wiener lab. CM 200 is a multitask system capable of performing 48 different tests to
48 samples in a walk-away manner.
Its purpose is to perform Laboratory Chemistry Analysis in automated and selective
form, either in routine or Stat mode. In Clinical analysis, it purpose is the In Vitro
Diagnostics.
It incorporates the latest in robotics, computer and communication technology to
render simple and reliable long-term operation.
The instrument consists of a system of modules performing specific functions,
computer controlled, and with bi-directional communication.
1.1 OVERVIEW
The system supports the following modules:

• PC/IBM compatible computer, Pentium 233 or higher, 256 Mbytes minimum
RAM memory, 40 Megs free space on hard drive, CD Rom
• 80-column dot matrix or bubble jet printer
• Cooled Sample/Reagent multiple tray
• Reaction tray
• Robot probe arm
• Diluter
• Photometer
• Level sensing systems
Multiple Sample/Reagent Tray. Loads 48 samples. Each sample can be positioned

consecutively or in any position in the tray. The system processes the samples in
increasing order, from 1 to 48.
The same tray accommodates 48 reagents; therefore, 48 single tests or 24 double
reagent tests, can be programmed for every sample.
Reagents are cooled by Pettier effect about 5 to 7oC below room temperature when
cooling unit is available on the model.
Reaction tray. 80 reaction well capacity. If the number of programmed reactions

exceeds 80 and automatic washer is not enabled or installed, the instrument will halt
and demand reaction cuvette replacement for specific positions, then will resume
operation. This routine repeats as necessary.
The reaction cuvettes are disposable and come in strips of five units and they are
available in 0.6 or 1 cm. of path length.
Robot probe arm. The robot probe aspirates the reagent and sample, introducing a
small air gap between them, then dispenses both in an identified reaction cuvette.
The probe arm thermostats both sample and reagent before dispensing, at 0.5 oC
above the selected reaction chamber temperature.
It has 4 work stages: (from right to left)
1. Dispensing position.
2. Wash position.
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3. Sample aspiration position.
4. Reagent aspiration position, reagents 1 to 24.
5. Reagent aspiration position, reagents 25 to 48 (only split reagent vials)
In the case of accidental probe arm collision, the system halts and signals an alarm
until the problem is cleared.
Diluter. A diluter with a 500-microliter syringe, aspirates reagent and sample
consecutively. Air gaps separate liquids to prevent early mixing and contamination.
Level sensors. When the probe aspirates samples or reagents, capacitive radio
frequency system senses liquid level and stops probe tip at the surface. The tip
penetrates the surface just enough to aspirate the required volume and minimizes the
possibility of carryover, contamination and volumetric error.
By the same means, the probe senses the level in all reagent vessels at the
beginning of the working cycle to establish if enough reagent is present for the
programmed assays.
Samples in primary tubes are used by the system, eliminating the need and risk of
sample transferring.
Impact detector. Whenever the probe tip or arm impacts a mechanical obstruction in
its path, it automatically halts, and visual and sound alarms are emitted. As soon as
the problem is cleared, the system will resume the job.
Photometer. The photometer is provided with 9-interference filters mounted in a filter

wheel, and has a double beam reference system. Wavelengths are: 340,380,
405,450, 505, 550, 600, 650, and 700 nanometers.
Light from a tungsten halogen source passes through the selected filter and a beam
splitter. One beam traverses the reaction cuvette and the other is directed upon the
reference detector. The reading is obtained as the ratio of both signals, and the
system is therefore immune to source fluctuations, exact filter positioning or dirt
accumulation on optical surfaces. This double beam design allows the detection of
reaction cuvettes in the reaction tray, and sets alarms if cuvettes are missing or
defective.
Bichromatic mode is enabled by the system. This consists of reading the sample at
two different wavelengths. The second wavelength is selected in a region where the
chromogen of the sample does not absorb. This accounts for turbidity, hemolysis and
intrinsic sample color, etc.
Cleaning system. In between sample aspiration, a programmable peristaltic pump

flushes the probe with distilled or de-ionized water (DI water) with tensioactive
addition. An alarm will flag when the DI water reservoir is almost empty, or when the
waste reservoir is almost full.
The consumption rate of the cleaning solution is very low.
The cleaning of the probe is enhanced by an additional automatic cleaning routine
accessed through the program with the aid of specific wash and soak solutions.
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Automatic cuvette washer. A four-step washer system allows reuse of cuvettes
until cuvette test fails. First station aspirates test liquids, second and third deliver
washing solution and empties cuvettes; fourth dry the cuvette.
1.2 OPERATING FEATURES

Output Data File: The results remain in the Sample Table unless they are erased or
sent to the History Table. Factors for each assay and results from control sera can
also be saved in separate tables in the History Table. The History Table is
automatically erased after a specified number of days, preset through Functional
Parameters Menu.
Printout of results: Analyzer uses all Windows printing capabilities. If data are saved
to disk, and no printout is required, set print batch parameter to zero.
Analytical Methods: Analyzer can store on hard disk an unlimited number of
different analytical methods. Methods can be: chemistry, coagulation, external or
calculated. Each chemistry method contains the following information:
Name: Up to 15 characters.
Test ID: 6 identification characters.
Nomenclature: A code number for identification by external software.
Brand: 15 characters for brand identification.
Type of assay:
Kinetics: Incubation and rate readings at intervals automatically pre-set.
End Point: Performs a blank reading in the reaction cuvette before
incubating the sample.
Color: Uses reagent blank. (One for each method).
Two point Kinetics: incubation and two readings at selected interval.
Reference:
Single point or multipoint (curve).
For single point factor and/or standard can be introduced.
Wavelength
Principal: Peak wavelength in clinical assays, wavelengths available in optic
parameters.
Bichromatic reference: For assays where serum color and turbidity interfere.
Improves precision of readings.
Sample volume: 2 to 100 microliters. (A parameter defines minimum allowed
volume)
First reagent volume: 0 to 1200 µl (1 cm cuvettes) or to 700 µl (0.6 cm cuvettes).
Second reagent volume: 0 to 450 µl. Use 0 for single reagent assays.
The sum of sample and reagents volumes should not exceed the reaction cuvette
capacity: 1200 ul for cuvette of 1 cm and 700 for cuvette of 0.6 cm.
Total volume must not exceed 1200 ml and 700 ul, respectively..
First incubation time.
Second incubation time: Used in double reagent methods. If this time is zero, both
reagents are loaded simultaneously.
Concentration of standards
Depending on the calculation type, system can operate with one or more
standards.
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One standard is used with normal colorimetric methods. When a high
measuring range is required in systems that do not obey Beer's Law; more standards
can be used either in Curve, in non-linear mode a quadratic or multilinear adjust.
Factor: If working with one standard, it will indicate the calculated factor in
accordance to concentration and absorbance of the standard. Not used in multipoint
calibration.
Initial absorbance Limits: Indicates reagent deterioration if limit is exceeded or is
below or above a specified maximum and minimum. Used with the reagent intensity
check option.
Threshold: For time readings (coagulation), it indicates absorbance change.
Limits
Low Concentration: Indicates concentration value that determines analysis
repetition when not reached.
High Concentration: Indicates concentration value that determines dilution and
repetition of analysis.
Consumption: indicates allowed maximum initial absorbance rate. Values above
it cause changes in the measuring interval and eventual repetition. Operates only in
kinetic modes.
Factor Calculation: Determines how the factor is handled. As options, a previously

calculated factor may be used, or an average between previous and actual factor can
be used, or simply use the actual factor.
Multipoint requires the use of multiple standards. They always operate in replacement
mode.
Reagent Tray: Designates a group of 1 to 48 reagent sets in the tray. The order is an
operator’s option. The position of each reagent in the tray, and the number present
are saved to memory as a “Reagent Tray.” An unlimited number of tray configurations
can be stored. It is not necessary to use all reagents in a tray. During a work cycle,
select the tray containing the required reagents. Just select the tray number or name
and its composition will appear on the screen.
Table of methods in use: is a sub-set of all methods. With a double click, methods
from this table go to selected entry in Sample Table.
Panels: they consist of sets of methods stored on a separate table. The use of
profiles saves time when data are introduced. When exported, all methods included
in that profile are sent to the Sample Table. It is normal practice to prepare a “Hepatic
profile”, a “Cardiac profile”, etc. In the Standard Table Profiles, Controls and
Standards are pre-defined.
STAT procedures: At any time, new samples can be input without interruption of
incubation times. Data can be entered via the Sample Table as “Samples” or as
“Stats.” When introduced as Samples, they are processed in the order that they
occupy in the tray. When introduced as Stats, they acquire priority over all Samples
already in the tray.
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Patient input: Patient data can be entered with their corresponding assay data of
each sample. The protocol number is mandatory. The name, age, sex and terminal
are optional.
Data are:
Protocol number
Surname and name
Age
Sex
Terminal (used for selective output/input)
Other optional demographic data)
Tests to be performed
These data will be displayed in the final report together with the assay results.
Statistics: Statistical analysis may be performed on samples, standards and

controls. They are performed through the History Table only. Levy-Jennings diagrams
are obtained and Westgard rules are applied.
Output data: Various printout formats are available. They are printed as samples are
completed. They include the Laboratory’s name, patient data, numerical data, units
and diagnostics. The export file is in Paradox or Dbase format.
Serial port communication: Data can be introduced and results returned from/to a
host computer with LIMS capabilities, through a RS232C serial port.
Transmission follows standards established in ASTM 1390 protocol.
1.3 TECHNICAL SPECIFICATIONS
NOTE: For the complete list of specifications, please refer to the user manual.
Samples
48 positions in rotary tray
Use of primary tubes or pediatric vials
Sample volume programmable 2 – 100 µl
Reagents
48 positions
Reagent volume programmable:
First reagent: 0 –700 ul
Second reagent: 0 – 450 µl
Typical volume: 300 µl for 1 cm cuvettes and 200 µl for 0,6 cuvettes.
Total volume (Sample + Reagent 1 + Reagent 2) must not exceed 1200
µl (Cuvette of size 1cm) and 700 µl (Cuvette of size 0,6 cm).
Reagents can be accommodated either in 50 ml. vials or double 30 ml
and 30 ml vials for 2-reagent methods.
Sampling system
Pre-heater in probe to deliver reagent at preset temperature
Capacitive sensor level
Inner and outer probe washing system
Diluter with valve assembly
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Reaction tray
Admits 80 cuvettes of 1cm or 0,6 cm of light pass
Double beam, interferential filters
Wavelengths: 340, 405, 450, 505, 550, 590, 650, 700 y 750 ó(380) nm.
Bandwidth: 10 nm
Photometric range: -0.1 to 3.6 A (-0.1 to 5.5 A with 0.6 cm cuvettes)
Lamp: halogen, 6 volts, 20 watts.
Analysis modes
End point with sample blank or reagent blank
Factor or standard
Priority programmable per sample (profile) or reagent (batch)
Calibration curves with two up to 10 standards
Automatic curve adjust
Turbidimetry
Coagulation time by turbidimetry
Fast and two-point kinetics (0 and 1st order)
Profiles, batches, STAT procedures.
Automatic time adjust and dilution with high substrate consumption,
Dilution for values above high limit.
Automatic repetition on abnormal low values.
Quality control: Levy Jennings plots, Westgard rules
Data export and import to other programs and/or remote terminals.
Automatic backup protection.
Data handling
Computer required: PentiumTM or equivalent
Minimum 256 Mb RAM (See Section )
Two serial ports RS232C or 1 serial port RS232C and 1 mouse PS2
Port. One additional serial port for communication with external LIMS
system.
Color monitor SVGA
CD Rom unit and 31/2” floppy disk
Ink jet or 80-column printer.
Communication
Serial port standard communication according ASTM 1394 protocol.
Supply
85 to 240 VAC +/-10% - 43/65 Hz – 400 VA
Automatic set
Fuses: 2.5 A – FF for 220 VAC
5.0 A - FF for 110 VAC µl
Insulation: Class 1
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Water consumption
1.4 ml/analysis, approximately
Usage mode
Continuous
WARNING: Instrument is Installation Category II. Instrument

requires protective ground connection. Verify ground connection
before installing the instrument
Main menu
The main menu bar contains menu drop-downs for all system functions and also icons to
directly access the most important functions.
Data
Methods: Analytical methods stored in memory
Samples: Table where samples, standards and controls are loaded.
Historic: All measured data can be sent to this table. Statistical calculations
can be performed on them.
Methods in Use: Table with a selected set of methods of daily use.
Panels: Table where standards, controls and profiles are pre-defined
Interferences: Sets of pairs of interfering reagents are defined.
Trays in Memory: Sets of reagents are stored in tray for easy load.
Trays
Samples and reagents: Graphic representation of Samples and Reagents
Tray. Allows operator to visualize programmed samples, reagents, volumes,
etc.
Movements
Manual
Automatic
Calibrate
Cleaning: Automatic probe cleaning procedure, purge and filling
Inspect
Communications: Contains all the communications between PC and
instrument for the last run.
Coordinates: Instantaneous values for last position of the system (Trays,
probe, read frequencies, etc)
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Status: Instantaneous status of error functions.
Messages: Warnings and error condition for the system. Some of them are
also shown in “ Operating conditions” and some are in the Error log file.
Errors: Error log file. Opens in WordPad format.
Filters: Gains, zeros, frequencies and status of the 9 optical filters.
Calibrations: Gains, zeros and frequencies for all gains, not only those
selected by the system.
Volumes: Once the samples are programmed, details of needed volumes for
all reagents. Error conditions also shown.
Priorities: Order of analysis is established by instrument. It takes into account,
highest priorities for blanks, next for standards, etc.
Times: Table showing all measurements in the reaction cuvettes. Also collects
information on actual measuring times, volumes, etc. It has an historic page
where data are stored when cuvettes are blanked.
Operating Conditions: Used cuvettes, samples to dilute, time to the next,
reading, status messages.
Parameters: (See Section ).
Miscellaneous
Repeat Analysis: Data of last reading are erased and Sample Tray re-loaded.
Clean Samples: Sample Table is erased. Sample Tray must be empty.
Clean Historic: Cleans Historic Table. Requires password.
Clean Messages: Cleans the Table of Messages.
Backup: Allows creation of backup files for Historic, Methods and Parameters.
Save Desktop: Saves settings on sizes, positions and columns for every
active window.
Print Screen: Direct printout of the active window.
Translator: Multi-language dictionary for all messages and screens.
Service: Access to service demo videos and descriptions stored in the
installation CD.
Help
Help Topics: Complete On-line help file.
What is new: version highlights.
About: Software version and manufacturer information.
Icons. Most of them correspond to already defined menus.
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Table of Methods
Table of Samples
Samples and Reagents Tray
Reaction Tray
Full initialization of system
Trays are disabled to facilitate load of samples and new cuvettes.
Manual movements
Ends all procedures in progress.
Start of automatic procedure.
Stops and resumes dispensing for Stat procedures
Times, volumes and priorities.
Prints active window
1.4 INSTALLATION
Please refer to user manual.
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2 ELECTRONICS
2.1 ANALYZER BOARDS:
1. M1401-P151: Keyboard and auxiliar display.
2. M850-P157: Sensor support for reaction tray and sample/reagent tray.
3. M852-P160: Double gain pre-amplifier for sample and reference
channel.
4. M2300-P202: Vertical movement sensor support.
5. M2300-P205: Temperature sensor support for reaction heater.
6. M2300-P206: Horizontal movement sensor support.
7. M2301-P213: Analyzer control CPU.
8. M2301-P216: Carrousel interfase: Calefactor controller for reaction tray;
temperature controller for robot calefactor.
9. M2301-P218: Power supply, switching supply of 5 VDC (step down), +/-
15 VDC supply; lamp supply.
10. M2301-P219: Interfase COMU: Variable gain amplifier/multiplixer and t/f
conversor for sample and reference channel; stepper motor controller for
photometer and washer.
11. * Level detector and serum/reagent calefactor.
12. M2301-P223: Washer/photometer sensor support.
13. M2301-P224: Interfase for barcode reader.
*: Might change depending the model of the instrument.

To obtain the code check price list.
ITEM BOARD QUANTITY COMMENTS

1 M1401-P151 - For testing purposes
2 M850-P157 2
3 M852-P160 2
4 M2300-P202 1
5 M2300-P205 1
6 M2300-P206 1
7 M2301-P213 1
8 M2301-P216 1
9 M2301-P218 1
10 M2301-P219 1
11 M2301-P222 1
12 M2300-P223 2
13 M2301-P224 1
21
2.2 GENERAL
The Wiener lab. CM 200 consists of a PC type computer and a robot system
commanded by two similar controllers with different programming and peripherals
personalized as COMU and CARRU.
COMU works as a master unit, receiving the computer commands through an
asynchronous RS232 serial port, and distributing the commands to all peripherals at
its charge: photometer 990, tray 850 (CARRU) and the diluter.
A serial port connects COMU, tray 850 and diluter. In this configuration, the
photometer 990 acts as an internal peripheral of COMU. To select a specific slave
peripheral, the message from the PC must include its address, defined by an ASCII
character.
Slaves can never start a message, they only reply when interrogated by the master.
COMU receives from the PC orders and status requests. These are distributed to the
tray 850 and the diluter. These are slaves of COMU, and COMU is a slave of the PC.
Communication protocol establishes messages of fixed length. Messages have a
heading, that include synchronism characters and the peripheral’s address, the
message and a 16 bits CRC. This CRC is used to test that information received by
the slave is correct. The slave returns a message of similar structure.
Photometer 990 is controlled by COMU directly through parallel lines to adjust gain,
timers for integration times, and a stepper motor controller for the filter wheel. The
halogen lamp is fed by a linear variable voltage power supply, adjusted through a
preset.
Tray 850 is also a peripheral controlled by COMU through an RS232 serial port, that
controls the reaction tray and sample/reagent tray stepper motors, the probe arm’s
stepper motors, (vertical and horizontal movements), and the peristaltic pump. It is
also in charge of setting the temperature controllers for the probe arm pre-heater and
the reaction chamber heater.
Acceleration ramps and velocities for movements are established by means of
parameters that can be modified connecting an auxiliary keyboard and display to
CARRU, and setting it to test mode. Temperature controllers are of P+I+D type, and
their parameters can also be modified with the auxiliary keyboard. All commands
executed by CARRU can be simulated.
The probe’s vertical and horizontal movements have initializing sensors and stop
sensors, additionally, the vertical movement has a capacitance level sensor and a
collision detector.
Both trays have an initializing sensor and a verification sensor that senses every 90
degrees of rotation. The peristaltic pump has no sensors.
Commands originated by the PC and destined to COMU or CARRU, can be of simple
or multiple type. Once a controller receives an order, it is occupied until the operation
is executed. Therefore multiple commands are diagrammed so movements can be
performed as fast as possible, and, in some instances, simultaneously with other
operations.
The internal structure of the messages is the same in both cases.
22
2.3 FUNCTIONAL DIAGRAM
SAMPLE VERTICAL HORIZONTAL

REACTION PERISTALTIC
REAGENTS PROBE PROBE
TRAY TRAY MOVEMENT MOVEMENT
PUMP
CARRU INTERFACE
M230X-P216
DILUTER
ISE CARRU CAVRO
XL-3000
CPU
SAMPLE
M230X-P213
PREAMPLIFIER
BAR
PHOTOMETER CODE
COMU
READER
REFERENCE
PREAMPLIFIER (BCR)
COMU INTERFACE
M230X-P219
2.4 COMMUNICATION PROTOCOLS

Three different protocols co-exist within the system. The one employed between
COMU and carrousel, the one between COMU and the PC, and the third between
COMU and diluter. This last one is defined by the OEM manufacturer.
2.4.1 COMMUNICATION PROTOCOL BETWEEN COMU AND DILUTER
All transmitted characters are ASCII.

Specifications are:
23
BAUD RATE 19200
DATA BITS 8
PARITY none
STOP BIT 1
MODE half duplex
The command block from COMU to diluter has the following structure:
1 START COMMAND BLOCK '/' 2Fh

2 PUMP ADDRESS 31h - 5Fh
N DATA BLOCK Length n
N+1 END COMMAND BLOCK <CR> Odh
The answer block is:
1 START ANSWER BLOCK '/' 2Fh

2 MASTER ADDRESS 30h
3 STATUS CHARACTER
N RESPONSE If applicable
N+1 <ETX> 03h
N+2 CARRIAGE RETURN 0Dh
N+3 LINE FEED 0Ah
N+4 LINE TURN AROUND CHARACTER
2.4.2 COMMUNICATION PROTOCOL BETWEEN COMU AND THE PC

A conventional serial port is used. PC’s COM1 or COM2 can be used. The PC
delivers commands and status requests. It performs as the master and the COMU
cannot communicate unless it has received instructions concerning one of the
peripherals that it controls.
The PC has a time-out that triggers after a command has been sent. If by any
chance the time-out is terminated, the system enters in prevention mode, and starts
sending status requests to the peripheral that did not answer in time. If no reply is
obtained, the system publishes an error message on the monitor screen.
COMU uses its RTS line to inform the PC of termination of the command. The PC
receives this information through its CTS line.
The communication from the PC towards CARRU has the following structure:
• Synchronism characters
• The address of peripheral receiving the command
• Command character
• Parameters associated to command
24
• CRC 16 BITS
COMU returns immediately the following message:
• synchronism characters
• address of peripheral who returns message
• command reception characters
• parameters associated to the command
• CRC 16 BITS
When the PC requests the status of any peripheral, the return message is as follows:
• synchronism characters
• address of the peripheral
• status characters
• parameters associated to the status request
• CRC 16 BITS
Three ASCII STX (002H) synchronism characters are used.

Peripherals addresses that the PC controls are:
PHOTOMETER '0' - 030H

TRAYS '1' - 031H
DILUTER '2' - 032H
ISE OPTION ‘3’- 033H
BARCODE OPTION ‘4’- 034H
2.5 COMMANDS FROM PC TO THE COMU
2.5.1 PROBE ARM ROBOT

Soft:
• Recalibration or initialization of the mechanism, (the PC must be aware of the time
this operation takes, and after that time it must perform a status request to know
operation results).
• Probe up (upper sensor).
• Probe down (lower sensor).
• Probe down until level is detected (it returns distance in steps to the lower sensor)
• Probe down N steps (if N is out of range, it performs nothing and informs error), it
returns the absolute position from the upper sensor.
• Probe up N steps (if N is out of range, it does nothing and informs error), it returns
the absolute position from the upper sensor.
• Horizontal position 1(reaction tray); it detects the right sensor.
• Horizontal position 2 (wash station); fixed quantity of steps starting from the right
sensor.
25
• Horizontal position 3 (sample vials); fixed quantity of steps starting from the right
sensor.
• Horizontal position 4 (reagents I); fixed quantity of steps starting from the right
sensor.
• Horizontal position 5 (reagents II); fixed quantity of steps starting from the right
sensor.
• Probe right N steps (if N is out of range, does nothing and informs error), it returns
the absolute position from the right sensor.
• Probe left N steps (if N is out of range, it does nothing and it informs error), it
returns the absolute position from the right sensor.
Hard:
• Number of steps between right and left sensor of horizontal movement.
• Number of steps between sensors of the vertical movement.
• Stored in EEPROM as positions 2, 3, 4, 5, 6 or 7, the present quantity of the
horizontal movement.
2.5.2 DILUTER
• Initialization of the diluter.
• Sample uptake speed.
• Reagent uptake speed.
• Delivery speed.
• Aspirate N steps with the syringe (the PC must know the relationship between
steps and microliters; the maximum quantity of steps that the CAVRO manages is
1000).
• Dispense N steps (same as previous).
2.5.3 PHOTOMETER 990

Soft:
• Photometer initialization, (filter wheel stepper motor, frequency/voltage converter)
• Position Filters (from 0 to 9)
• Read frequency
• Read frequency with filter N and gain M (with filter from 0 to 9 and gain from 0 to
31)
• Change the integration time
Hard:
• Delay between the filter wheel steps
2.5.4 REACTION TRAY

Soft:
• initialization of the reaction tray
• positioning in cup N (with N from 0 at 79)
26
• advance N cups (it returns the new position, from 0 to 79; it is independent of the
quantity of requested cups and of the position where it is)
• go back N cups (same as previous).
Hard:
• Stored in EEPROM by the use of the auxiliary board are the steps between two
consecutive sample positions and the differential position of samples and reagents.
2.5.5 SAMPLE / REAGENTS TRAY

Soft:
• Initialization of the samples/reagents tray
• Positioning in sample N (with N from 0 to 47)
• Positioning in reagent N- sector I ( N from 0 to 23)
• Positioning in reagent N – sector II (N from 24 to 48)
Hard:
• Stored in EEPROM by the use of the auxiliary board are the steps between two
consecutive sample positions and the differential position of samples and reagents.
2.5.6 PERISTALTIC PUMP

Soft:
• advance N steps (with N from 1 to 4000)
• back N steps (with N from 1 to 4000)
• complete cycle of pump, comprised by two similar movements separated by an
interval of time in which the pump stops
Hard:
• Storage drop-back and period values
2.6 DESCRIPTION OF THE MESSAGES
The message that PC sends to COMU, is:
0 1 2 3 4 5 L H
STX STX STX COM COM DIR MESSAGE (12 CAR) CRC CRC
(02) (02) (02) (xx) (xx) (xx) (xx) (xx)
6 7 8 9 10 11 12 13 14 15 16 17
where:
STX: character ASCII STX (02 - 002H).
COM: command character in ASCII (see Table I).
DIR: address character of the peripheral to which the command is destinated.
CRC: CRC of 16 bits.
27
MEN: command parameters(characters in ASCII).
With exception of the synchronism characters, all sent characters are in ASCII format
(from 020H up to 07FH).
The routine that is used for the CRC calculation written in C Language is :
/ * ROUTINE THE CRC CALCULATES * /

int crc (char cant_b, char *punt)
{
unsigned int crc_p, odd;
unsigned char and, j;
crc_p = 0xffff;
for (and = 0; and <cant_b; y++)
{
crc_p ^ = *punt;
for (j = 1; j <= 8; j++)
{
odd = crc_p & 0x01;
crc_p >> = 1;
if (odd)
crc_p ^ = 0xa001;
}
punt++;
}
return (crc_p);
}
The controllers in Analyzer are only receivers.

They do not return any message unless they are interrogated by the PC. In case
the PC demands the controller’s status, the corresponding command is issued.
From the PC’s point of view, the COMU works in such a way that every time the PC
commands, it answers immediately. This answer is affirmative or negative. In the
negative case, the message includes the reason. The reasons for which a command
is not accepted are:
• error of CRC 16 bits: the PC resends the command.

• busy peripheral: the PC waits until it is ready.
• several errors: - parameters wrong.
- peripheral does not exist.
- command does not exist.
If the message is accepted, the PC waits for a period equivalent to the length of the
expected action and then request status of the peripheral. This status has three
alternatives:
• busy peripheral: the PC waits.

• peripheral error: the PC publishes an error message.
• peripheral ready: the requested command was correctly completed.
28
If the COMU detects an incomplete message, or a message without the heading, it
will not answer. This will generate a time-out in the PC and the request resent.
2.7 COMMUNICATION PROTOCOL BETWEEN COMU AND CARRU
These commands are used by COMU to control CARU CPU.

The communication protocol used between the CARRU and the COMU is similar to
the used by the PC and the COMU. The CARRU CPU must respond in time to the
COMU. If an error is detected, for example a motor does not respond, once checked,
it informs the COMU.
All messages have a time-out that starts when the first character of the message is
detected. This time-out is 0.2 sec. longer than the total transmission time at a definite
baud rate. If the time-out terminates, the COMU triggers a sequence of error
detections . CARRU uses no interruptions for the control of the serial ports. It just
enables multiprocessor communication mode.
The commands employed by COMU to control functions are:
• Sample/reagent tray initialization

• Reaction tray initialization
• Horizontal movement initialization
• Vertical movement initialization
• Peristaltic pump initialization
• Reagent to position XX (0 to 23)
• Reagent to position XX (24 to 47)
• Sample to position XX (0 to 47)
• Reaction tray to position XX (0 to 79)
• Probe, upper rest position

• Probe, lower rest position
• Probe, up XXX steps
• Probe, down XXX steps
• Probe, lower until it senses level
• Probe, to reagent position

• Probe, to sample position
• Probe, to wash station
• Probe, to reaction tray position
• Probe to horizontal position of Reagents I

• Probe to horizontal position of Reagents II
• Probe to horizontal position of Samples
• Probe to washing horizontal position
• Probe to reaction horizontal position
• Probe to horizontal position of ISE
29
• CARRU status request
• COMU status request
• Diluter status request
• ISE status request
• Code bar status request
2.8 DESCRIPTION OF SERIAL PORT COMMANDS
A detailed list of all commands the Analyzer can receive are:
TABLE I - COMMANDS OF PC TO COMU
COD. DIR. COMMAND PARAMETERS Ans.

' IS' '1' Sample/reagent tray initialization None (2)
' IR' '1' Reaction tray initialization None (2)
' IV' '1' Probe, vertical movement initialization None (2)
' IH' '1' Probe, horizontal move. initialization None (2)
' IB' '1' Peristaltic pump initialization None (2)
' IC' '2' Diluter initialization None (2)
' IF' '0' Photometer initialization None (2)
' VU' '1' Probe up N steps at slow speed (150 2 (hexa) digits (2)
hz), when command is initiated. (optional)
' VD' '1' Probe to lower rest position None (2)
' VN' '1' Probe senses level. If already there, an 2 (hexa) digits (2)
optional parameter is the number of (optional)
steps from upper rest position.
' Vu' '1' Probe up N steps 3 digits (HEXA) (2)
' Vd' '1' Probe down N steps 3 digits (HEXA) (2)
' H1 ' '1' Probe to position 1 (reaction tray) None (2)
' H2 ' '1' Probe to position 2 (wash station) None (2)
' H3 ' '1' Probe to position 3 (sample position) None (2)
' H4 ' '1' Probe to position 4 (reagent position) None (2)
' H5 ' '1' Probe to position 5 (reagent 2 of double None (2)
reagent)
‘ H6 ‘ ‘1‘ Probe to position 6 (ISE) None
‘ H7 ‘ ‘1‘ Probe to position 7 (reagent 1 of double None
reagent)
' Hr' '1' Probe to the right N steps 3 digits (HEXA) (2)
' Hl' '1' Probe to the left N steps 3 digits (HEXA) (2)
' Ss' '1' Sample tray to position N 2 digits (2)
' Sr' '1' Reagent tray to position N 2 digits (2)
' Sv' '1' Sample/reagent tray vibrate (E= 3 digits (HEXA)+3+2 (2)
30
amplitude, N= number of cycles,
T=period)
' Rn' '1' Reaction tray to position N 2 digits (2)
' Ra' '1' Reaction tray advance N cups 2 digits (2)
' Rr' '1' Reaction tray reverse N cups 2 digits (2)
' Rv' '1' Reaction tray vibration (E=amplitude in 3 (HEXA) + 3 + 2 digits (2)
steps; N=number of cycles, T= period)
' RA' '1' Reaction tray advance half cup None (2)
' RR' '1' Reaction tray reverse half cup None (2)
‘AU ' ‘1 ' UPS shutdown (turns off the Analyzer 3 digits (2)
and the PC) within T seconds
' Ff' '0' Photometer, set filter N and gain M 1 + 1 digit (3)
' FL' '0' Photometer frequency reading, with 1 + 1 + 2 digits (3)
filter N, gain M and integration time O
' FF' '0' Sample frequency reading with 2 digits (3)
integration time O
' FR' '0' Reference frequency reading with 2 digits (3)
integration time O
' Fl' '0' Reference frequency reading with filter 1 + 1 + 2 digits (3)
N, gain M and integration time O
' Du' '2' Syringe plunger move relative N steps 4 + 2 digits (4)
up with M velocity
' Dd' '2' Syringe plunger move N relative steps 4 + 2 digits (4)
down with velocity M
' Da' '2' Move syringe plunger to absolute 4 + 2 digits (4)
position N with velocity M
' E0 ' '0' Photometer status None (3)
' E1 ' '1' Carru status None (2)
' E2 ' '2' Diluter status None (4)
' e1 ' '1' Carru instantaneous status None (2)
'EM' '0' System status after a multiple None (5)
command
'Ba' '1' Peristaltic pump, rotate 4 + 4 digits (HEXA) (2)
counterclockwise N + M steps (N and M
can be zero)
'Br '1' Peristaltic pump, rotate clockwise N + 4 + 4 digits (HEXA) (2)
M steps (N and M can be zero)
'BA' '1' Peristaltic pump, rotate 4 + 4 digits (HEXA) (2)
counterclockwise N + M steps and then
clockwise the number of steps stored in
EEPROM
'Bm' '1' Peristaltic pump rot. clockwise M and 4 + 4 + 2digits (HEXA) (2)
performs O cycles of N oscillation steps
31
(back and forth)
' @d ' '1' Disable CARRU motors 1 optional digit (2)
' @e ' '1' Enables CARRU motors 1 optional digit (2)
' DR' '2' Diluter valve to REAGENT position None (4)
' DS' '2' Diluter valve to SAMPLE position None (4)
' L1' '1' Enable output for bar code reader None (2)
' L0 ' '1' Disable output for bar code reader None (2)
In commands Vu1, Vd1, Hr1 and Hl1 the parameters in ASCII HEXADECIMAL.
The command @d1 is used to put the Analyzer in the energy saving mode, lowering
the lamp voltage and shouting down energy to all motors.
In the commands @d1 and @e1, the optional parameter is: 0-REACTION, 1-
SAMPLE/REAGENT, 2-HORIZONTAL, 3-VERTICAL or 4-PUMP, in ASCII, indicates
which motor is to be ENABLED/DISABLED.
When commands MI1 + IF0 are performed, all Analyzer modules are initialized.
In commands Ba1, Br1, BA1 and Bm1, parameters must be given in ASCII
HEXADECIMAL.
NOTES:
1. You have to take care with half cup advance and reverse commands. The
space between two cups is 27 steps with CZ motor but 30 with UM motor.
There is no problem with UM motor because half space is 15 steps. With CZ
motor the tray advance only 13 steps and this is not balanced if you order
another half cup advance. You have to order a reverse command or go to a
specific cup. If the probe go down when the tray is in an intermediate position
the 2300 with give a collision error.
2. For carrousel immediate status request, you have to follow this sequence: Ask
an immediate status request (e11) and then a normal status (E11). The
immediate status command is like other normal command and not a particular
status. COMU asks CARRU about its states and CARRU gives an answer.
That is the reason why you have to do a double operation. It is used after
dispensing into reaction tray to test if the probe is wet.
3. In case the CARRU returns all status bits (ST1) as high, it means that COMU
has not received an answer for one of the transmitted commands, so COMU is
not answering. Please, try again for three times. COMU waits one minute
maximum for CARRU to answer. This is like that because some carrousel
commands may last long time, specially those commands that use the
peristaltic pump.
32
4. Advance and reverse half cup: When PC orders CARRU to advance half cup,
CARRU returns the same cup number where it was. After this command, you
have to use the half cup reverse command for the plate to stay in the previous
position. When PC orders half cup reverse and it is placed in a whole cup, the
CARRU returns the same cup number where it was minus one.
5. PREAMPLIFIER M851-P160 and AMPLIFIER M2301-P219:

The Analyzer use two M851-P160 boards for the SAMPLE and REFERENCE
channels. These preamplifiers generate two analogical signals, one in high and
the other in low. This is defined with bit 3 of the output port of the 2681-0 (U8).
To select which channel, the bit 2 is used.
The M2301-P219 amplifier uses a 16 channel multiplexer . 16 different gains
are obtained. The used bits of the output ports of the 2681-0 (U8) are 4, 5, 6
and 7.
GAIN CONTROL TABLE
HEXA BIT 7 BIT 6 BIT 5 BIT 4 GAIN LOW HIGH

LEDS OC3 OC2 OC1 OC0 OUTPUT GAIN GAIN
CODE MUX16 ASCII ASCII
F 1 1 1 1 0 '0' 30H '@' 40H
E 1 1 1 0 1 '1' 31H 'A' 41H
D 1 1 0 1 2 '2' 32H 'B' 42H
C 1 1 0 0 3 '3' 33H 'C' 43H
B 1 0 1 1 4 '4' 34H 'D' 44H
A 1 0 1 0 5 '5' 35H 'E' 45H
9 1 0 0 1 6 '6' 36H 'F' 46H
8 1 0 0 0 7 '7' 37H 'G' 47H
7 0 1 1 1 8 '8' 38H 'H' 48H
6 0 1 1 0 9 '9' 39H 'I' 49H
5 0 1 0 1 A ':' 3AH 'J' 4AH
4 0 1 0 0 B ';' 3BH 'K' 4BH
3 0 0 1 1 C '<' 3CH 'L' 4CH
2 0 0 1 0 D '=' 3DH 'M' 4DH
1 0 0 0 1 E '>' 3EH 'N' 4EH
0 0 0 0 0 F '?' 3FH 'O' 4FH
NOTE: GAIN -> ON = LOW

OFF = HIGH
CHANNEL -> ON = SAMPLE
OFF = REFERENCE
CHANNEL CONTROL AND MODE

CODE HEXA BIT 3 BIT 2 MODE AND CHANNEL
HI/LO CHANNEL
3 1 1 LOW (SAMPLE)
2 1 0 HIGH (SAMPLE)
33
1 0 1 LOW (REF.)
0 0 0 HIGH (REF.)
The number of combinations produced by these two charts is 64. There are 32 gains
for each channel. These 32 gains are grouped in 16 HIGH and 16 LOW. Both groups
partially overlap.
The codes range from 0x30 to 0x4f in hexadecimal and correspond, in the ASCII
chart, with codes for the numbers (0 to 9), to symbols (:; <=>? @) and letters (‘A’ to
‘O’).
If the code is smaller than 0x40 (symbol @), the COMU decides that the gain is low,
and if it is equal or larger than, the gain is high.
In the case of multiple commands, two places in the message are used for the gains:
one for the sample channel and other for the reference channel.
2.9 MULTIPLE COMMANDS, GENERAL
The multiple commands perform automatically a sequence of simple commands:
1) To position probe (horizontal movement) in sample and to position sample tray;

the multiple command is formed by:
Ss1, H31 and (2) characters.
2) The same, but positioning in reagents; the sequence is:
Sr1 H41 and (2) characters (reagents 1 to 12)
or Sr1 H51 and (2) characters (reagents 13 to 18)
3) The same, but positioning in reaction cups; the sequence is:
Rn1, H11 and (2) characters.
4) To dispense two different volumes at different speeds; the sequence is:
Du2, Du2 and (4 + 2 + 4 + 2) characters.
5) To read the frequency of the sample channel and the frequency of the reference
channel with a given filter, given gain, integration time and delay, after moving the
filter wheel and another delay between the two measurements; the sequence is:
Fd0 = FF0, FR0 and (1 + 1 + 1 + 2 + 2 + 2) characters.
6) Same as previous, but positioning in the reaction tray; before reading:
Rn1, FF0, FR0 and (1 + 1 + 1 + 2 + 2 + 2 + 2) characters.
7) The STATUS OF A MULTIPLE COMMAND, only acts when a command that
involves two different modules is requested. For the time being, the only command
that carries out an operation that uses two modules is the ' M60 '.
TABLE II - MULTIPLE COMMANDS OF PC TO COMU
COD. DIR. COMMAND PARAMETERS Rta.

' MI ' '1' CARRU initialization None (2)
‘ M0 ‘ ‘1‘ Probe to pisition 6 (ISE) and reaction tray
to position N
' M1 ' '1' Probe to position 3 and sample tray to 2 digits (2)
34
position N
' M2 ' '1' Probe to position 4 and reagents tray to 2 digits (2)
position N
' M3 ' '1' Probe to position 1 and reaction tray to 2 digits (2)
position N
' M4 ' '2' Syringe plunger up N steps at velocity M 4 + 2 + 4 + 2 digits (4)
and up N' steps at velocity M' (Absolute
position)
' M5 ' '0' Photometer frequency reading, sample 1 + 1 + 1 + 2 + 2 + 2 digits (3 ')
and reference channels with F, G, G', IT, t
and t' (t and t': delays)
' M6 ' '0' Photometer frequency reading, sample 1+1+1+2+2+2+2 (3 ')
and reference channels with F, G, G', TI, digits
t, t' (t and t' delays) and reaction tray in X
‘M7 ' ‘1 ' Empty cup with peristaltic pump while 3 digits (HEXA) (6)
descending the probe to the step X
‘M8 ' ‘2 ' Empty cup with the diluter while 3 + 4 + 2 digits (HEXA the (4)
aspirating a volume D at a speed V. first one)
‘M9 ' ‘1 ' Horizontal movement of the probe and 3 + 1 + 2 digits (HEXA the (2)
rotation of H steps of the S/R tray, first one)
direction: S and sample number: SR.
‘MA ' ‘0 ' Simultaneous sequence of dilution and 4+2+2+1+1+1+1 (3 ')
reading with R diluter steps, at a speed V digits
and rotation of reaction tray to position
PR, with filter F, gains GS and GR and
integration time TIC; then, after
aspiration, probe moves to upper position
and syringe plunger moves 20 steps up.
‘MB ' ‘0 ' Simultaneous sequence of probe wash 4(HEXA) + 2 + 2 + 1 + 1 (3 ')
and photometer reading with B steps of + 1 + 1 digits
peristaltic pump and reading parameters
equal to previous described command,
(‘MA ').
‘MC ' ‘0 ' Multiple movements employed in ELISA 3(HEXA)+ 3(HEXA) digits (2 ')
assays to access wells in micro-well
plates. Parameters are probe horizontal
steps and S/R tray position in steps.
‘MD ' ‘0 ' Similar to the ‘MA0’ command, but for the 4+2+2+1+1+1+1 (3')
S/R tray, after diluter terminates digits
operation, probe is moved to horizontal
position 1
'ME' '0' Simultaneous sequence of photometer 2+2+2+1+1+1+1 (3')
reading and probe positioning to reagent digits
N, and lower sensing level, then lowering
35
another M steps. (Used for single
reagents or position 2 of double reagent).
‘ Me ‘ ‘0‘ Simultaneous sequence of photometer 2+2+2+1+1+1+1 (3’)
reading and probe positioning to reagent digits
N, and lower sensing level, then lowering
another M steps. (Used for going to
position 1 of double reagent).
'MF' '0' Same as before, but probe sent to 2+2+2+1+1+1+1 (3')
Sample S digits
'WM' '0' Complete cuvette wash cycle, including 1 + 2 digits (3')
the number of washes in the cycle and
flushing time, ( this is expressed as
XX*100). Drying pump operates
continuously.
‘WD’ ‘0’ Down washing arm (3’)
‘Wd’ ‘0’ Cycle of washing filling pump 2 digits (3’)
‘WU’ ‘0’ Up washing arm (3’)
'MG' '0' Complete dispensing cycle, air gap 3 + 2 + 2 + 2 + 1 + 2 digits (3')
performed by peristaltic pump and
simultaneous cuvette wash cycle.
‘MH’ ‘0’ Simultaneous probe wash and 2+3+2+1+1+1+1 digits (3’)
photometer reading
‘MJ’ ‘0’ Same as ‘MF0’ but with command Vd1 in 2+3+2+1+1+1+1 (3’)
place of VN1 digits
‘MK’ ‘0’ Simultaneous dispensing and peristaltic 3 (HEXA) + 2 + 2 digits
pump induced air gap.
‘ML’ ‘0 Washing sequence, with reaction tray 2+2 (3’)
position and filling time.
‘MM’ ‘0 Simultaneous sequence of empty syringe 3 (HEXA) + 2 + 2 + 1 + 1 + (3')
and reading 1 + 1 digits
2.9.1 DESCRIPTION OF MULTIPLE COMMANDS
1. INITIALIZE ALL MODULES IN SIMULTANEOUSLY.

byte
MI1
2. PROBE TO POSITION 6 (ISE) AND THE REACTION TRAY TO POSITION N:

byte 6 7
M01 N N'
3. PROBE TO POSITION 3 AND THE SAMPLE TRAY TO POSITION N:

36
byte 6 7
M11 N N'
4. PROBE TO POSITIONS 4 AND REAGENT TRAY TO POSICIÓN N:

Byte 6 7
M21 N N'
5. PROBE TO POSITION 1 AND THE REACTION TRAY TO POSICIÓN N:

Byte 6 7
M31 N N'
6. MOVE SYRINGE TO POSITION N WITH VELOCITY M AND GO TO

POSITION N' WITH VELOCITY M':
Byte 6 7 8 9 10 11 12 13 14 15 16 17
M42 N3 N2 N1 N0 M1 M0 N3 ' N2 ' N1 ' N0 ' M1 ' M0 '
7. SAMPLE AND REFERENCE FREQUENCIES READINGS, WITH FILTER F,

SAMPLE GAIN G, REFERENCE GAIN G', INTEGRATION TIME TI, DELAY t
AND DELAY t':
byte 6 7 8 9 10 11 12 13 14
M50 F G G' TI1 TI0 t1 t0 t1 ' t0 '
8. SAMPLE AND REFERENCE FREQUENCIES READINGS, WITH FILTER F,

SAMPLE GAIN G, REFERENCE GAIN G', INTEGRATION TIME OF BOTH
CHANNELS TI , DELAY t, DELAY t' AND REACTION TRAY IN POSICIÓN X:
byte 6 7 8 9 10 11 12 13 14 15 16
M60 F G G' TI1 TI0 t1 t0 t1 ' t0 ' X1 X0
where:
F: filter.
G: gain of the sample channel.
G': gain of the reference channel.
TI: integration time.
t: delay after positioning of filter wheel, adjustment of the sample
gain and selection of sample channel.
t': delay after sample channel measurement, adjustment of reference gain,
selection of the reference channel and reading of reference channel.
X: position of the reaction tray.
9. EMPTY A CUP WITH THE PERISTALTIC PUMP

Byte 6 7 8
M71 X2 X1 X0
This command waits until the PROBE is positioned above the CUP to be emptied.
Probe descends until level is detected and then the peristaltic pump begins to sip.
37
Simultaneously the probe descends progressively and stops when vertical position
X2, X1, X0 is reached. This value is in ASCII HEXADECIMAL.
The command returns the status, and the quantity of steps the pump backed.
0 1 2 3 4 5 6 7 8 9
STX STX STX ST0 ST1 DIR PB3 PB2 PB1 P01
02H 02H 02H 31H
ERRORS:
• If level is not detected and the probe moves the predetermined steps, no return
error code is generated, but pump returns zero steps.
• If level is sensed and pump actuates 20000 steps and level is still detected,
implying pump malfunction, not error code is returned, and pump returns 20000
steps as its status.
10. EMPTY A CUP WITH THE DILUTER:

This command waits until the probe is positioned above the cup to be emptied. Probe
descends until level is detected and then the syringe aspirate to empty the cup while
the probe lowers progressively until diluter reaches the requested steps in the
command, with parameter D3,D2,D1,D0 and with speed V1, V0, and X2, X1, X0 is
the lowest position the probe reaches.
byte 6 7 8 9 10 11 12 13 14
M80 X2 X1 X0 D3 D2 D1 D0 V1 V0
11. HORIZONTAL MOVEMENT OF PROBE ARM AND ROTATION OF SAMPLE/

REAGENT TRAY:
Parameters received are the steps the probe moves horizontally, left or right. It also
receives the position for the sample tray.
byte 6 7 8 9 10 11
M91 H2 H1 H0 S SR1 SR0
Where H2, H1, H0 are steps to move in the direction S (left = l , right = R and SR1,
SR0 is the position of the sample tray.
12. SIMULTANEOUS SEQUENCE OF REAGENT TAKE-UP AND PHOTOMETER

READING:
This multiple command performs two simultaneous tasks, to aspirate reagent with
diluter and to measure both photometer channels. Once the command is received,
the COMU sends the order to the DILUTER to move syringe plunger up, returning a
termination status. Then gives the order to CARRU to locate REACTION tray in
position PR1, and PR0. While this occurs, it places the filter F the in the
PHOTOMETER . When REACTION tray and PHOTOMETER are in the requested
positions, a measurement of both channels with the integration time TIC, (CODED
INTEGRATION TIME), is performed.
38
Byte 6 7 8 9 10 11 12 13 14 15 16 17
MA0 R3 R2 R1 R0 V1 V0 PR1 PR0 F GM GR TIC
When done, COMU emits an answer of type ‘3'.
13. SIMULTANEOUS SEQUENCE OF PROBE FLUSHING AND PHOTOMETER

READING:
This multiple command carries out two simultaneous tasks: flushing the probe with
the peristaltic pump and carrying out a complete measurement for both channels.
Once the command is received, COMU sends the order to CARRU to position the
probe in the washing station and to the pump to flush, while REACTION tray is
positioned. The COMU also moves filter wheel and adjusts requested gain for each
channel. When REACTION tray and PHOTOMETER are in position, the COMU reads
both channels with the integration time TIC (CODED INTEGRATION TIME).
COMU generates MB1 command sending it to CARRU.
When CARRU moves the REACTION tray, it picks the RTS signal, which COMU
reads through CTS, and lowers it as soon as tray is positioned. While CARRU moves
the REACTION tray, COMU positions the filter wheel. Readings for both channels are
performed after CARRU lowers the RTS line and COMU completes filter setting.
byte 6 7 8 9 10 11 12 13 14 15 16 17
MB0 B3 B2 B1 B0 SR1 SR0 PR1 PR0 F GM GR TIC
14. SIMULTANEOUS MOVEMENTS OF PROBE AND S/R TRAY FOR MICRO-

WELL OPERATION:
This command is used for ELISA assays. Two parameters are received: horizontal
probe steps and S/R tray position relative to initialization position.
byte 6 7 8 9 10 11
MC1 H2 H1 H0 SR2 SR1 SR0
HORIZONTAL STEPS S/R TRAY STEPS
HEXADECIMAL HEXADECIMAL
15. SIMULTANEOUS SEQUENCE OF PHOTOMETER READINGS AND SAMPLE

ASPIRATION.
This command is similar to MA0, but for the SAMPLE position.

Once sample aspiration is performed, probe ascends and moves to position 1.
Byte 6 7 8 9 10 11 12 13 14 15 16 17
MD0 R3 R2 R1 R0 V1 V0 PR1 PR0 F GM GR TIC
REACTION TRAY
DILUTER STEPS DILUTER POSITION WHEN FILTER GAIN GAIN
VELOCITY READING SAM. REF..
39
When status is requested, COMU send a type ‘3’answer.
16. SIMULTANEOUS PHOTOMETER READINGS AND PROBE MOVEMENT TO

POSITION 4.
This command is split into a ‘Rn1’and a ‘ME1’. This last one is a command between
COMU and CARRU. The PC cannot send it. COMU sends the ‘Rn1’ first and waits for
the CARRU to answer. Then COMU reorders the buffer and gives CARRU the
command ‘ME1’. Next it triggers the photometer readings. Once done, it verifies
CARRU has finished.
byte 6 7 8 9 10 11 12 13 14 15 16 17
ME0 PR1 PR0 PV1 PV0 0x20 0x20 PR1 PR0 F GM GR TIC
REACTION TRAY GAIN GAIN
REAGENT STEPS BELOW NOT USED POSITION WHEN FILTER SAM. REF.
POSITION LEVEL READING
The status request is responded with type ‘3 ‘answer.

Command ‘ME1’ is structured:
byte 6 7 8 9
ME1 PR1 PR0 PV1 PV0
REAGENT STEPS BELOW
POSITION LEVEL
17. SIMULTANEOUS SEQUENCE OF PHOTOMETER READINGS AND

LOCATION OF PROBE IN POSITION 3:
Similar to previous but for the sample position.
byte 6 7 8 9 10 11 12 13 14 15 16 17
MF0 PS1 PS0 PV1 PV0 0X20 0X20 PR1 PR0 F GM GR TIC
STEPS BELOW REACTION TRAY GAIN GAIN
SAMPLE LEVEL NOT USED POSITION WHILE FILTER SAM. REF.
POSITION READING
The status request to COMU is responded with type ‘3 ‘answer.

Command 'MF1' is structured:
byte 6 7 8 9
MF1 PS1 PS0 PV1 PV0
SAMPLE STEPS BELOW
POSITION LEVEL
18. COMPLETE CUVETTE WASH CYCLE
40
Parameters sent are number of washes per cycle, the flushing pump time, (2 decimal
characters). This time is multiplied internally by 100. If this time is zero, pump will not
operate.
byte 6 7 8
WM0 CL PB2 PB1
NUMBE PUMPING
R OF TIME
CYCLES
19. CUVETTE WASHING DOWN ORDER
byte
WD0
20. CYCLE OF WASHER FILLING PUMP

It performs a filling cycle in the time given as a parameter (2 decimal characters).
It internally multiplies for 20.
byte 6 7
Wd0 T2 T1
empty time
21. CUVETTE WASHING UP ORDER
byte
WU0
22. SIMULTANEOUS DISPENSING AND CUVETTE WASHING.
The instrument expects probe to be in position 1. Then receives the command.

COMU generates the command “Vd1 V2 V1 V0”, and probe lowers V2,V1,VO steps.
Syringe plunger is moved to position 0, (upper rest position), at speed VD1,VD0.
Then the peristaltic pump generates an air gap in the probe tip. Next probe moves
PB1 PB0 steps towards position 1 and lowers a number of steps stored in EEPROM
of CARRU. Command BA1 of CARRU is used to perform this.
After dispensing cycle, probe is moved to position 2.
This is performed while a cuvette wash cycle takes place. This cycle consists of
flushing two cups with distilled water, then lowering the multiple washer while sample
and water are aspirated by the pumps that start to operate, and a drying block
connected to a vacuum pump dries the last cup. Then pumps stop as washer lifted
out of cuvettes. This operation can be replicated several times within a cycle, defined
by the CL parameter.
41
byte 6 7 8 9 10 11 12 13 14 15 16 17
MG0 V2 V1 V0 DV1 DV0 PR1 PR0 PD1 PD0 CL DL1 DL0
PROBE, STEPS DILUTER REACTION TRAY PERISTALTIC REPETI FLUSHING
DOWN VELOCITY POSITION PUMP STEPS TIONS TIME
MG0 command sequence:
Reaction Command Fill Wait for Command Lower Empty cups Lift washer Wait for
Tray sent to cups Answer to diluter to washer from cups Diluter to
positioning CARRU from move up (1) answer
t(Vd1) CARRU the probe (Da2)
(Vd1)
Filling cycle Empty cycle
Fill cups Send Lower washer Empty cups Lift washer Waits for N times
command answer, cuvette
(H21) to CARRU washing
CARRU, (H21)
Filling cycle Empty cycle Empty cycle
(1) If error is detected when lifting washer, dispensing is also performed.

If the number of washes per cycle is greater than 2, COMU performs the washes
consecutively with no other movement in the meanwhile.
If washer gives an error, this command finishes the simultaneous dilution.
23. SIMULTANEOUS PROBE EXTERNAL WASHING AND PHOTOMETER

READING
byte 6 7 8 9 10 11 12 13 14 15 16 17
MH0 PV2 PV1 PV0 DV1 DV0 0X20 PR1 PR0 F GM GR TIC
INT.
STEPS DOWN VERTICAL PROBE EXT REACTION TRAY FILTER GAIN GAIN TIME
MOVE MENT WASHER VALVE POSITION WHILE SAM. REF.
DELAY READING
COMU sends to CARRU the following message:
byte 6 7 8 9 10 11 12
MH1 PV2 PV1 PV0 DV1 DV0 PA1 PA0
STEPS DOWN VERT PROBE EXT STEPS UP FOR
MOVEMENT WASHER VALVE VU1 AT LOW
DELAY SPEED
Once this command is received, CARRU has to perform the following commands:
42
H21, Vd1(PV2, PV1, PV0), Hv1 (DV1, DV0) y VU1
With those commands it performs the probe external washing. After that, it
communicates to COMU the command is finished.
24. SIMULTANEOUS PHOTOMETER READINGS AND PROBE MEVEMENT TO

POSITION 3
Similar to command MF0 but with level sensing replaced for PROBE DOWN a certain
number of steps.
byte 6 7 8 9 10 11 12 13 14 15 16 17
MJ0 PS1 PS0 PV2 PV1 PV0 0X20 PR1 PR0 F GM GR TIC
INT.
SAMPLE PROBE, STEPS NOT REACTION TRAY FILTER GAIN GAIN TIME
POSITION DOWN USED POSITION WHILE SAM. REF.
READING
COMU responds a type ‘3’answer to a status request.

Command 'MJ1', is structured:
byte 6 7 8 9 10
MJ1 PS1 PS0 PV2 PV1 PV0
SAMPLE PROBE,
POSITION STEPS DOWN
25. DISPENSING AND AIR GAP, MULTIPLE COMMAND
This command expects probe in coded position 1. If not, it automatically generates

the H11 command and sends it to CARRU. COMU generates a command Rn1 with
PR1,PR0, then a command Vd1 with V2,V1,V0 steps down.
Next it orders diluter to move plunger to position 0 at speed VD1,VD0 and set valve in
psition PUMP.
After this, it sends command H21 to stop probe in coded 2 position.
byte 6 7 8 9 10 11 12 13 14
MK0 V2 V1 V0 VD1 VD0 PR1 PR0 PD1 PD0
VERTICAL STEPS DILUTER SPEED REACTION CUP PERISTALTIC
DOWN FOR WHERE TO PUMP STEPS
(HEXA) DISPENSING DISPENSE
26. CUVETTE WASHING CLEANING SEQUENCE
This command rains the first cuvette washing tubing with clean water. Former, it
places the reaction tray in PR1,PR0 cup. After that it fills the cups in the washing
position. It executes the Rr102 command to reverse the plate two positions. For five
43
consecutive times it performs the cups dry sequence. Between sequences, it
executes the command Ra01 to advance one cup.
byte 6 7 8 9 10
ML0 PR1 PR0 DL1 DL0 CL
REACTION TRAY CUVETTE WASH QTY.
POSITION FILLING TIME
When finishes it does not perform the last reaction tray advance.
27. SIMULTANEOUS SYRINGE EMPTY AND READING
former it performs a Vd1 command to carry the probe to the required vertical position.
Then it gives the diluter an order to carry the syringe to 0 position. Immediately and
without diluter answer, it gives CARRU the Rn1 order to place the reaction tray and
automatically the filter wheel. Once the filter wheel is placed, it waits CARRU to finish
the plate positioning and start readings.
byte 6 7 8 9 10 11 12 13 14 15 16 17
MM0 PV2 PV1 PV0 VD1 VD0 0X20 PR1 PR0 F GM GR TIC
INT.
STEPS DOWN VERTICAL DILUTER REACTION TRAY FILTER GAIN GAIN TIME
MOVE MENT SPEED POSITION WHILE SAM. REF.
READING
2.10 INTEGRATION METHOD

The PHOTOMETER uses the following method to integrate the signal:
TRAY FILTER GAIN_M CHANEL_M DEL_t READ_M GAIN_G ' CHANEL_R DEL_t ' READ_R
it repeats as many times as TI/5
The tray is only moved with the “M60” command.

ANSWERS TO COMMANDS FROM THE ANALYZER
2.10.1 GENERAL
The answers have been coded in the following way:
Answer 1
When the COMU receives the command it responds at once accepting it. The PC
waits until CTS is high, then requests the status of the instrument and is informed if
this command has been completed or not.
0 1 2 3 4 5 L H
STX STX STX ST0 ST1 DIR MESSAGE (12 CHAR) CRC CRC
(02) (02) (02) (xx) (xx) (xx) (....) (xx) (xx)
MESSAGE: (12 characters)
The initialization status is returned even though not required by the PC.
44
2.10.2 CARRU
The answers have been coded in the following way:
Answer 2
When the COMU receives the command, it responds immediately accepting it. The
PC waits until CTS is high, and then requests the state of the Analyzer. In the
message returned by the COMU, the coded absolute positions of the peripheral are
sent.
0 1 2 3 4 5 L H
(02) (02) (02) (xx) (xx) (xx) (xx) (xx)
6 7 8 9 10 11 12 13 14 15 16 17
PV2 PV1 PV0 PH2 PH1 PH0 R/S RS1 RS0 R1 R0 phc

In the case of an answer type (2) - CARRU:
6,7,8: probe vertical position
9,10,11: probe horizontal position
12: R/S (coded R or S) tray position
13,14: R/S (cup) tray position
15,16: reaction tray position
17: coded horizontal position (1, 2, 3, 4, or 5; if 0 means not in normal
operation position).
In the case that the multiple command M71 has been sent, the COMU returns:
6 7 8 9 10 11 12 13 14 15 16 17
PB3 PB2 PB1 PB0
where Pbi are the pulses the peristaltic pump rotated as requested in the command.
If a temperature command is given, the COMU answers:
6 7 8 9 10 11 12 13 14 15 16 17
P PS1 PS2 PS3 PS4 PS5
2.10.3 PHOTOMETER (STANDARD COMMAND)

Answer 3
When the COMU receives the command, it responds accepting it. The PC waits until
CTS is high, and then requests the status of the instrument, the reply indicates if the
command was successful or not .
0 1 2 3 4 5 L H
(02) (02) (02) (xx) (xx) (xx) (xx) (xx)
45
6 7 8 9 10 11 12 13 14 15 16 17
F7 F6 F5 F4 F3 F2 F1 F0 F G TI1 TI0
(3) 6,7,8,9,10,11,12,13: value of the read frequency

14: used filter
15: used gain
16, 17: integration time
In the case of a command reading both channels the returned status is:
2.10.4 PHOTOMETER (MULTIPLE COMMAND):

Answer 3 '
6, 7, 8, 9,10, 11: value of the reference frequency
12,13,14,15,16, 17 : value of the sample frequency
NOTE: the COMU returns this answer structure only the first time the status is
requested after receiving a multiple command in which readings are made for the
SAMPLE AND REFERENCE channels.
2.10.5 DILUTER
Answer 4:
When the COMU receives the command it responds accepting it .The PC waits until
CTS is high and then requests the status of the instrument, where it is informed if this
command has been completed successfully or not .
0 1 2 3 4 5 L H
(02) (02) (02) (xx) (xx) (xx) (xx) (xx)
6 7 8 9 10 11 12 13 14 15 16 17
D3 D2 D1 D0 V1 V0 Rta

And in the case of an answer type (4) - DILUTER:
6,7,8,9: syringe plunger position.
10,11: syringe plunger speed.
17: answering character corresponding to the Diluter
2.10.6 MULTIPLE COMMANDS:

When the Analyzer requests the status after the execution of a MULTIPLE
COMMAND, it replies different from the normal mode. The description of the bits of
46
ST0 and ST1 should be consulted in the section DESCRIPTION OF STATUS
BYTES.
2.11 DESCRIPTION OF STATUS BYTES
First byte of STATUS (ST0):
7 6 5 4 3 2 1 0
ST0 (byte 3) CRC PAR COM CAR TPR PER BL BD
where:
bit 7: 1 = wrong CRC
bit 6: 1 = wrong parameters
bit 5: 1 = inexistent command
bit 4: 1 = CRC error in the 16 bits parameter memory of CARRU
bit 3: 1 = REACTION tray cover open
bit 2: 1 = BUSY PERIPHERAL
bit 1: 1 = DISTILLED WATER vessel empty
NOTE: CARRU returns to the COMU in this bit, the state of the SPI bus. When
0, means no problems. If 1, means CARRU has detected a communication
problem with one of the SPI controllers.
bit 0: 1 = DRAINAGE VESSEL full
NOTE: CARRU returns to COMU in this bit, the status of the UPS. When 0,
UPS is not operating. If 1, UPS is running the instrument on batteries.
(UPS {0002} with batteries on).
Second STATUS byte (ST1):

7 6 5 4 3 2 1 0
ST1 (byte 4) OE6 OE5 OE4 OE3 OE2 OE1 OE0 EP
where:
bit 7: 1 =
bit 6: 1 =
bit 5: 1 = source of the error
bit 4: 1 = (SEE CHART below)
bit 3: 1 =
bit 2: 1 =
bit 1: 1 =
bit 0: 1 = PERIPHERAL ERROR
ST1 PHOTOMETER CARRU DILUTER

bit 7 - OE6 ACTIVATED UPS (2) LEVEL SENSOR ACTIVATED UPS (2)
bit 6 - OE5 WASHER PERISTALTIC PUMP ISE BUSY (1)
47
ST1 PHOTOMETER CARRU DILUTER
bit 5 - OE4 PROBE VERTICAL MOV. ISE ERROR_HOST (1)
bit 4 - OE3 PROBE HORIZONTAL MOV VALVE OVERLOAD
bit 3 - OE2 V/F CONVERTER SAMPLE/REAGENT TRAY SYSINGE OVERLOAD
bit 2 - OE1 FILTER WHEEL REACTION TRAY NO ANSWER
bit 1 - OE0 COLLISION DETECTION INTERNAL PARAMETERS
bit 0 - EP ERROR ERROR ERROR
(1): they inform ISE module status.

(2): When the UPS is activated, the COMU returns to the PC the bit 7 of byte ST1 in
high, for both the PHOTOMETER and the DILUTER.
2.12 DESCRIPTION OF THE STATUS BITS OF ISE MODULE AND BAR CODE
READER
ST1 ISE CODE BAR READER

bit 7 - OE6
bit 6 - OE5
bit 5 - OE4
bit 4 - OE3
bit 3 - OE2
bit 2 - OE1
bit 1 - OE0 REQUEST TO HOST
bit 0 - EP ERROR ERROR
2.13 NOTES ON THE DETECTORS
2.13.1 COLLISION DETECTOR

The collision detector is read the moment the PC sends to CARRU the order to
lower the PROBE. This reading is carried out every time a pulse is sent to the stepper
motor in charge of vertical movement. It is also read at the initiation of the horizontal
movement of the probe. This means that there is no collision detection during the
execution of horizontal movement. When a collision has been detected, it is
necessary to re-initiate the horizontal and vertical movements.
If, when an initialization is ordered, the probe ascends to the upper rest position, the
collision sensor is still active, peripheral error and collision detection bits are
reset.
48
Whenever a status request is sent to CARRU, the collision sensor state is the
actual.
2.13.2 CAPACITIVE LEVEL DETECTION

The level sensor is only questioned while lowering the probe, or seeking for level
or for the lower rest position sensor. Commands to lower probe a given quantity
of steps, do not sense level.
Commands for ascending the probe any number of steps, or to the upper rest
position, can be performed even if the probe tip is submersed in liquids.
If the command VD1 is used and level is sensed, the CARRU returns an error, and
bits 0 and 5 of ST1 are activated. If command VN1 is used and no level detected, the
same situation arises.
2.13.3 UNINTERRUPTED VOLTAGE SUPPLY (UPS)

The analyzer can be fed by a UPS.
During power fail, the analyzer receives through its auxiliary serial port RS232C, the
report that analyzer is fed by UPS batteries. In case the UPS is BEST - FORTRESS,
it can be automatically active. The PC, when informed, (bit 7 of the byte ST1),
proceeds to interrupt dilutions and devotes to measure samples that have been
diluted until that moment.
It is convenient at this time, to execute the commands @d11, @d12, @d13, to
disable the motors of the SAMPLE TRAY, HORIZONTAL and VERTICAL ARM. (This
is performed through system software).
Using a UPS IF1020 (1020VA), the complete analyzer and computer, has an
autonomy of 15 to 20 minutes, per battery.
The PC software constantly reads bit 7 of byte ST1 of the photometer status.
If main power is restored, dilution are continued and full operation continues.
Once the analyzer finishes measuring, if mains power has not been restored,
command AU1 is executed, and a complete shut-down is performed to protect UPS
batteries. It is important to have closed all files in the PC to avoid the risk of
information loss.
2.14 STATUS ORDERS

Next, status obtained after analyzer initialization are shown:
2.14.1 PHOTOMETER
In the case of simple command:
0 1 2 3 4 5 L H
STX STX STX ST0 ST1 0 MESSAGE (12 CHAR) CRC CRC
02H 02H 02H XXH XXH 30H XXH XXH
49
6 7 8 9 10 11 12 13 14 15 16 17
0 0 0 0 0 0 0 0 0 0 2 0
30H 30H 30H 30H 30H 30H 30H 30H 30H 30H 32H 30H
FREQUENCY T.INT.
F: filter number
G: gain
TI: Integration time (20 = 1 second)
In the case of multiple command:
0 1 2 3 4 5 L H
6 7 8 9 10 11 12 13 14 15 16 17
0 0 0 0 0 0 0 0 0 0 0 0
30H 30H 30H 30H 30H 30H 30H 30H 30H 30H 30H 30H
REFERENCE FREQUENCY SAMPLE FREQUENCY
2.14.2 CARRU
0 1 2 3 4 5 L H
6 7 8 9 10 11 12 13 14 15 16 17
0 0 0 0 0 0 S 0 0 0 0 1
30H 30H 30H 30H 30H 30H 53H 30H 30H 30H 30H 31H
VERTICAL HORIZONTAL R/S SAMPLE V. REAG. V. PHC
VERTICAL: vertical position in steps from the probe upper rest position.
HORIZONTAL: horizontal position in steps from the right sensor.
PHC: coded horizontal position (0 means other positions than 1, 2, 3, 4
or 5).
2.14.3 DILUTER
0 1 2 3 4 5 L H
50
6 7 8 9 10 11 12 13 14 15 16 17
0 0 0 0 0 0 ‘
30H 30H 30H 30H 30H 30H 20H 20H 20H 20H 20H 60H
STEPS SYRINGE SYR.SPD. Free Ans
STEPS SYRINGE: position of the syringe.

SYRINGE SPEED: speed of the syringe plunger.
Answer: answer of the dilutor
answer of the dilutor (d = incorrect position of the syringe plunger)
2.15 DIGITAL TEMPERATURE CONTROLLERS
The analyzer has two microprocessor temperature controllers: one for the reaction
tray chamber heater, and one for the probe’s S/R pre-heater.
TABLE III - TEMPERATURE CONTROLLER COMMANDS

# COD. ADD DESCRIPTION PRINCIPAL SECONDARY Ans.
R. PARAMETER PARAMETERS
'TR' '1' Select reaction tray temperature 'T' '000' + 2 digits
'TR' '1' Unable reaction tray heating 'J'
'TR' '1' Read reaction tray temperature 't'
'TC' '1' Select probe pre-heater temperature 'T' '000' + 2 digits
'TC' '1' Unable probe pre-heater operation 'J'
'TC' '1' Read probe pre-heater temperature 't'
To select a temperature for the reaction tray, the command is:
byte 6 7 8 9 10 11
'TR1' 'T' '0' '0' '0' T1 T0
TEMPERATURE
IN THE 25 -37 ºC
RANGE
For the probe pre-heater, the command is:
byte 6 7 8 9 10 11
'TC1' 'T' '0' '0' '0' T1 T0
TEMPERATURE
IN THE 32- 47 ºC
range
The answer to a temperature request is:
0 1 2 3 4 5 L H
51
STX STX STX ST0 ST1 1 MENSAJE (12 CHAR) CRC CRC
6 7 8 9 10 11 12 13 14 15 16 17
t 0 X X Y Y
74H 30H XXH XXH YYH YYH 20H 20H 20H 20H 20H 20H
TEMPERATURE TEMPERATURE
ENTEGERS DECIMALS Free
2.16 INTERFERENCE FILTERS

Interference filters used by instruments are:
POSITION FILTER (nm) BW (nm) COMMENTS

0 blocked -- blind
1 340 10
2 405 10
3 450 10
4 505 10
5 546 or550 10
6 590 or 600 20
7 650 10
8 672 or 700 10
9 767 or 380 20
2.17 CALIBRATION AND TEST PROGRAMS
2.17.1 AUXILIARY BOARD M1401-P151
52
L8
J1
M1401-P151
ON
ON
L7 L6 L5 L4 L3 L2 L1 L0
7 8 9 ESC A
4 5 6 DEL B
1 2 3 YES C
. 0 ENT NO D
2.17.2 COMU TEST PROGRAM

To enter the Carru test program, DIP SWITCH 1 of the auxiliary board should be
OFF, and DIP SWITCH 2 should be ON. The rest of the DIP SWITCH should be
OFF .
Next, the screen displays:
CPU 860 to See X.XX

Test Program
2.17.2.1 Comu commands menu
In COMU test mode with the auxiliary board connected, when you press NO key
KEY 0: communication test for the channel A of the 2681.
KEY 1: position 0 of the filter wheel of the photometer.
KEY 2: advances one filter position in the photometer.
KEY 3: backs one filter position in photometer.
KEY 4: reading of the selected channel and gain control :
key 0: gain changes from 0 to 15.
key 1: gain change, HIGH/LOW.
key 8: change of integration time (20 = 1 second).
53
key 9: toggles sample (S1) and reference (S2) channels
key C: advance to the next filter.
key D: select filter zero.
key ESC: return to the command menu.
KEY 5: keyboard tests; returns to command menu with period key.
KEY 6: diluter valve operation, (OPEN/CLOSE).
KEY 7: cuvette washer control menu:
Key 0: washer UP/DOWN
Key 1: actions during a period delay_time the two aspiration
pumps.
Key 2: powers cuvette flushing pump during volumen_bomba
time.
Key 3: cuvette aspiration cycle comprised by: aspiration pump
powering, lowering washer, a time period defined by
delay_valve, and elevating washer and pump shut-down.
Key 4: complete flushing-aspirating cycle. Same as before but
with time volumen_bomba.
Key 5: complete flushing-aspirating cycle. Same as before but
lowering washer before activating aspiration pumps.
Key 6: sends command TR1I to CARRU, (initialization of
reaction tray temperature controller).
Key 7: sends command TR1T00037, (set 37°C), to CARRU.
Key 8: washing cycle with tray movement. The delay variable
expresses the wash cycle time. After 7 cycles, the reaction tray
moves to next position until ESC is keyed.
Key 9: Wash (5) cuvettes since the key-entered position.
Key A: Channel A-1 serial port test
Key B: Channel B-1 serial port test
Key C: ISE test by PC serial port. Escape by pulsing CPU.
Key D: ISE test programs, ISE_control ()
key 0: send command I
key 1: enter date (day/month/year) and send command D!
key 2: Send command C!
key 3: Send command S!
key 4: Send command W!
key 5: Send command M!
key 6: Send command K!
key 7: Send command c?
key 8: Send command d?
key 9: Send command E!
key A: Send command A!
key B: Send command B!
key C: Send command 1000p!
key D: Send command -1000p!
key YES: Send command P!
key PERIOD: Send command L?
key NO: Send command L?, I? and 5 cycles A
54
measurement.!
key ESC: Out of ISE test menu
KEY 8: filter wheel positions in filter number selected by keyboard.

KEY 9: control of the diluter syringe, enter step number to of desired position.
KEY DEL: Move syringe plunger to position 0.
KEY YES: Move syringe plunger to position 1000.
KEY ESC: initialization of the CAVRO XL 3000 diluter.
KEY ENTER: status request to the CAVRO XL 3000 diluter.
KEY A: reads in continuous and simultaneous mode, the two photometer channels
(sample: M and reference: F) of the amplifier (M851-P159 and M851-P160 boards):
key 0: gain change from 0 to 15.
key 1: gain change HIGH/LOW.
key 8: integration time change (20 = 1 second).
key 9: returns to frequency mode.
key A: reading of zeros for both channels, frequencies are
displayed.
key B: reads blank for both channels and displays frequencies.
Every ten readings, the maximum differences are displayed. All
values displayed are converted from frequency to absorbance
values.
key C: advances to the next filter.
key D: positions filter zero.
key NO: selects single or double mode.
key YES: resets readings counter to zero.
key PERIOD: loads the lap times t_esp_1 and t_esp_2 after
positioning the filter wheel and after reading each channel.
key ESC: returns to the command menu.
KEY NO: COMU parameters, refer to table further on.
KEY B: erases initialization parameter list of the EEPROM. When erased, the next
initialization is performed with default parameters.
KEY C: toggles lamp control (LAMP HIGH and LAMP LOW).
KEY D: gets the CARRU control from COMU.
key 0: initializes the CARRU.
key 1: advances one cuvette position in reaction tray.
key 2: advances one vial position in sample/reagent tray.
Key 3: peristaltic pump control. The number of steps to rotate
is input. Diluter valve positions, command M1100
generates, , probe stops at
position 2 and lowers 500 steps, and pump rotates the
inputted steps.
key 4: Purges hydraulic system. Diluter valve is set, probe
stops at position 2 and lowers 100 steps, the peristaltic
pump rotates 30000 steps. Probe moves upwards.
key 5: Probe moves horizontally to the entered position.
key 6: Probe vertical movement toggle.
55
key 7: Order to read with BCR with parameter BCR_mode = 1
(software)
key 8: Sequential reading of N vials with programmed delay
between the order of tray advance and BCR reading.
key 9: BCR order reading with BCR_mode parameter = 0
(hardware).
Key ESC: Go back to command menu
KEY PERIOD (DOT) : Send to CARRU all printable characters in ASCII code.
SWITCH A: Enter lamp value by auxiliary keyboard.
SWITCH B: Lamp parameter value: lamp_high
SWITCH C: Lamp parameter value: lamp_low
SWITCH D: Lamp to ZERO.
NOTE: Default values are mentioned below just for reference, for the instrument
parameters, refer to the parameter sheet located on the instrument cover.
ITEM VARIABLE DEFAULT DESCRIPTION

1 time_com 600 time-out between characters received by PC
2 time_filtro 150 Delay between pulses sent to photometer stepper

motor.
3 time_brazo 40 Delay between pulses sent to washer stepper motor.
4 time_comMB 400 Delay between CARRU’s answer and the initiation
of measurements in the simultaneous command
MB0.
5 vol_bomba 20 Delay during which flushing pump is activated. This
value is internal to COMU. The value employed in
normal use is sent by the PC.
6 delay_valve 10 Delay during which washer aspiration pumps are
activated.
7 vel_com 1 Communication velocity between the analyzer and
the PC. For 0, → 9600 bauds.
For 1, → 19200 bauds.
8 vel_cavro 12 Final velocity of CAVRO diluter. Internal use of the
COMU test program.
9 const_vel 270 Conversion constant between CAVRO diluter
models SB 1200 and XL 3000.
10 pasos_comMA 10 Steps CAVRO XL 3000 diluter moves after reagent
take-up in the MA0 simultaneous command. This
parameter must be twice air gap volume set in PC
internal parameters.
11 lav_en 0 Enables (2) or disables (0) the cuvette washer.
12 pasos_VU1 30 Steps in simultaneous MH0 command, probe exits
liquids at slow speed.
13 codigo_enable 0 Enable (1) or disable (0) the bar code reader
14 ISE_enable 0 Enable (1) or disable (0) the ISE module
56
15 lamp_low 1000 Initialization DAC value (lamp control)
16 lamp_high 4000 DAC written value for tests with auxiliary board
17 day 15 ISE internal use
18 month 3 ISE internal use
19 year 02 ISE internal use
2.17.2.2 Comu normal mode

When COMU is in normal mode, after a command is sent to the PHOTOMETER, the
following information is displayed :
CPU 860 Ver X.XX

User Program
Gain: 0 Filter: 0
Freq: 0 Tint: 20
After a command is sent to the CARRU, the following information is shown on

display:
CPU 860 Ver X.XX

User Program
S: 0 R:10 S C:1
PH: 0 PV: 0
After a command is sent to the DILUTER, the following information is displayed:
CPU 860 Ver X.XX

User Program
Vel: 12 Pos: 0
2.17.3 TEST PROGRAMS FOR CARRU
For CARRU test program, DIP SWITCH 1 of auxiliary board should be ON.
The following screen appears:
CARROUSEL - KEYBOARD
Version 3.05
0:PARAM 1:SENSORS
2:COMMAND 3:SEQ. MOV.
57
With keys 0 to 3, the following modes are accessed :
KEY 0: Set motors parameters.
KEY 1: Sensors control.
KEY 2: Simulate CARRU Commands.
KEY 3: Automatic sequence of CARRU Movements.
To return to previous screen, press ESC, (valid for all test program menus).
2.17.3.1 Parameter set mode
When key 0 is pressed, the following information appears on screen, indicating that
the system is in parameters set mode
Key 0: Reaction tray parameters.
- SET PARAMETERS
- REACTION TRAY
KEY 1: parameters of the sample/reagent tray.

KEY 2: parameters of probe horizontal movement.
KEY 3: parameters of probe vertical movement.
KEY 4: parameters of the peristaltic pump.
When key 0 is pressed, the following information appears on screen, indicating that
the system is in reaction tray mode:
-SET PARAMETERS
-REACTION TRAY
In the case that the keys 1, 2, 3 or 4, are pressed, the display shows :
(Key 1) –SAMPLE/REAGENT TRAY
(Key 2) -HORIZONTAL PROBE
(Key 3) -VERTICAL PROBE
(Key 4) –PUMP
In general, in these command modes, the following keys are used:
KEY 0: tolerance parameter (MODES 0, 1, 2 and 3: values from 0 to 255) or

recovery parameter (MODE 4: value from 0 to 255).
KEY 1: parameter period (MODE 0, 1, 2, and 4: value from 0 to 255), or
frequency parameter (MODE 3: value from 0 to255).
KEY 2: starting period parameter (MODE 0, 1, 2 and 3, values from 0 to
58
255). Mode 3 is read only.
KEY 3: ramp steps parameter (MODE 0, 1, 2 and 3: value from 0 to 255).
Parameter 3 is read only.
KEY 4: distances between sensors parameter (MODE 0, 1, 2 and 3: value
from 0 to 65535).
KEY 5: parameters that indicate stop horizontal position of probe (MODE 2).
When key 0 is pressed the following information appears on the screen:
-SET PARAMETERS
-REACTION TRAY
Tolerance: xx
When pressing the keys 1, 2, 3 or 4, it is shown on the display:
Period: xx
Init. Period :xx
Ramp Steps: xx
Distance: xx
For this command the following keys are used:

KEY 0: steps for the tolerance (value from 0 to 255).
KEY 1: period (value from 0 to 255).
KEY 2: starting period (value from 0 to 255).
KEY 3: ramp steps (value from 0 to 255).
KEY 4: distance between sensors (value from 0 to 65535).
KEY 5: offset S/R tray: distance between initial position and center of sample
1.
In the case of the PROBE HORIZONTAL MOVEMENT, key 5 is added to input the
steps for the corresponding fixed positions . Display shows:
- SET PARAMETERS
- HORIZONTAL PROBE
Position #
With keys 0, 1, 2, 3, 4 or 5, the desired fixed position values are introduced. The
display shows:
Position #0: xx
In the case of the PERISTALTIC PUMP, only keys 0 and 1 are used. With key 0 the
air gap steps can be adjusted, and with the key 1 the period is adjusted .
Each time a value different from the existing one is input, a message is appears:
NVRAM? Y/[N]
59
When answering YES, this value is entered in non volatile memory.
In the case of the VERTICAL PROBE MOVEMENT, frequency parameter entered is
multiplied by 100, ( for example, default value for frequency is 20, this means that
final frequency is 2000 Hz).
2.17.3.2 Sensor control mode

In main menu, strike key 1 to display:
-SENSOR SEEK
0:R 1:S 2:H 3:V
Pressing key 0, screen displays:
Position: xxxx yy
-REACCIÓN TRAY
0:In 1:Z 2:V
where yy is the error value, in hexadecimal, of the corresponding variable.

Pressing key 1, screen displays:
Position: xxxx yy
- REACCIÓN TRAY
0:CCW 1:CW
Every time that keys 0 or 1 are pressed, the requested operation is performed.
To return to the previous menu, press ESC.
While in sensor seek menu, if key 3 is pressed, screen displays:
Position: xxxx yy
- VERTICAL PROBE
0:In 1:Z 2:V 3:L
2.17.3.3 Command simulation mode
When key 2 is pressed in main menu
- COMMAND SIMULATION
0:R 1:S 2:H 3:V 4:P
5:I 6:M 7:S 8:T 9:U
60
If key 0 is pressed, screen shows:
CARRUSEL–KEYBOARD
-REACTION TRAY
0:n 1:a 2:b 3:c 4:A
KEY 0: moves the tray to cuvette N.

KEY 1: advance N cuvettes.
KEY 2: back N cuvettes.
KEY 3: vibration routine. Once selected, parameters Elongation, Quantity, (number
of vibrations), and Period, they can be changed .
KEY 4: advance half distance between cuvettes.
KEY 5: back half distance between cuvettes.
If starting from the command simulation menu, key 1 is pressed, screen reads:
CARRUSEL –KEYBOARD
-SAMPLE/REAGENT TRAY
0:s 1:r 2:v
P:XXXX B:XX E:X p:YY
where p can be S or R, indicating to which sector of the tray yy refers.

KEY 0: to move the tray to Sample N.
KEY 1: to move the tray to Reagent N.
KEY 2: vibration routine. Once selected, parameters can by modified: Elongation,
(vibration amplitude); Quantity, (number of vibrations) and
Period, (time interval between steps).
In simulation command menu, if key 2 is pressed, screen displays:
CARRUSEL–KEYBOARD
-HORIZONTAL PROBE
1-5 6:r 7:l 8:v
P:XXXX B:XX E:XX #Y

KEY 1: to position #1.
61
KEY 6: moves probe N steps to the right.
KEY 7: moves probe N steps to the left.
KEY 8: open washing cuvette valve.
While in command simulation menu, key 3 is pressed, screen displays:

VERTICAL PROBE
0:U 1:D 2:L 3:u 4:d
P: XXX B: XX E: XX

KEY 0: moves probe up till the upper sensor.
KEY 1: moves probe till the lower sensor.
KEY 2: moves the probe till liquid level.
KEY 3: moves the probe up N steps.
KEY 4: moves the probe down N steps.
While in command simulation, key 4 is pressed, screen diplays:
CARROUSEL KEYBOARD
-PUMP
0:a 1:r 2:A 3:m
E: XX

KEY 0: advance N + M steps.
KEY 1: back N + M steps.
KEY 2: advance N+M steps and back a number of steps stored in the air gap
parameter of the pump.
KEY 3: massage routine. Once selected, Retrieve, Elongate and Quantity can be
changed.
If in menu of command simulation the key 5 is pressed, screen displays:
-INITIALIZATION
0:R 1:S 2:H 3:V 4:P
XX XX XX XX XX
Where XX is the error variable for each movement.

62
KEY 0: initializes Reaction tray.
KEY 1: initializes Sample/Reagent tray.
KEY 2: initializes Probe Horizontal Movement.
KEY 3: initializes Probe Vertical Movement.
KEY 4: initializes Peristaltic Pump.
If in menu of command simulation the key 6 is pressed, screen displays:

MULTIPLE COMMANDS
0:I 1-3 , 7-9, B, C
R: XX S: XX H: XX V: XX

KEY 0: multiple initialization.
KEY 1: moves Sample/Reagents tray to vial N and the probe to position #3.
KEY 2: moves Sample/Reagents tray to reagent N and the probe to position #4.
KEY 3: moves Reaction tray to cuvette N and the probe to position #1.
KEY 7: activate pump till step XXX of probe vertical movement.
KEY 8: aspirates with diluter til step XXX of probe vertical movement.
KEY 9: rotates sample/reagent tray to sample position S and probe horizontally
XXX steps to right or left.
KEY B: moves sample/reagent tray to position S, then move reaction tray to cuvette
N,
and rotate peristaltic pump XXXX steps.
KEY C: rotates sample/reagent tray to step XXX and probe horizontally to step
YYY.
KEY Y: rotates sample/reagent tray to reagent cuvette R and probe to position 4,
then
lower probe until it senses level, and down XX additional steps.
KEY N: rotates sample/reagent tray to sample S and probe horizontally to position
3, lower to sense level, then XX additional steps.
KEY DEL: Lowers probe XXX steps.
Striking key 7 in command simulation menu, the screen displays:
-CARROUSEL STATUS
[00] [00] 000000S00001
Press a key
where the shown status corresponds to the last multiple initialization. To return to the
menu of simulating commands, press any key.
If in the command simulation menu, the key 8 is pressed, screen displays:
63
CARRUSEL KEYBOARD
-TEMPERATURE
R-> 0:I 1:T 2:J 3:t
P-> 4:I 5:T 6:J 7:t
Where R stand for reaction tray heater and P for the probe pre-heater. For this
command, the keys used are:
KEYS 0 and 4: Controller initialization.

KEYS 1 and 5: set XX in centigrade degrees.
KEYS 2 and 6: disables temperatures.
KEYS 3 and 7: read temperatures.
Striking key 9 in the command simulation menu, the screen displays:
CARRUSEL –KEYBOARD
-UPS TURN OFF
Seconds: XX
Striking key A:
CARRUSEL – KEYBOARD
-ENABLE MOTORS
Y:e N:D
In this command the following keys are used:
KEY Y: then with keys 0 to 4, the corresponding motors are enabled, and with key A,
all motors are enabled.
KEY N: same as before, but to disable.
2.17.3.4 Automated sequence test mode
When in the main menu, the key 3 is pressed
Automatic Sequence
[1] / [2] / [3] / [4] / [5]
64
Each sequence perform a series of functions:
1: A complete dilution cycle without level sensing in the S/R tray and with reaction
tray random rotations to balance incubation temperature for all cuvettes. Displays
on
screen the number of cycles performed and the number of detected errors.
2: A complete dilution cycle with level sensing in S/R tray, and reaction tray
stationary. Displays on screen the number of cycles performed and number of
errors detected.
3: Probe vertical movement test sensing level. If key 0 is stricken, probe will
ascend 40 steps after level detection, if key 1 is selected, probe ascends to the
upper rest position once it sensed level. Displays step number of both positions
and cycle number.
4: Probe senses level for each of the 4 possible horizontal positions. Display
shows the number of cycles performed and errors encountered.
5: Tests trays in random movements. Striking 0 reaction tray moves, striking 1, the
S/R tray is tested. The number of cycles and error are displayed.
All sequences are aborted striking ESC.
2.17.3.5 Carrusel in normal mode
When in normal mode, on the screen the following information appears:

R: xxxx e:yy l:zz
S: xxxx e:yy l:zz
H: xxxx e:yy l:zz
V: xxxx e:yy l:zz
Where:
R: reaction tray.
S: sample/reagents tray.
H: horizontal movement.
V: vertical movement.
e: masked state of the buffer E3 of the board M850-P149 at error moment.
l: image in RAM of the latch E4 of the board M850-P149.
and
xxxx: steps from the zero position.
yy: state of the buffer E3.
zz: state of the latch E4.
2.17.3.6 Carru test menu tree
NOTE: Default values are mentioned below just for reference, for the instrument
65
parameters, refer to the parameter sheet located on the instrument cover.
0 - SET PARAMETERS
0 - REACTION TRAY
0 - Tolerance 10
1 - Period 4
2 - Init. Period 15
3 - Ramp Steps 44
4 - Distance 600
5 - Home Offset 0
1 - SAMPLE/REAGENT TRAY
0 - Tolerance 10
1 - Period 6
2 - Init. Period 24
3 - Ramp Steps 36
4 - Distance 500
5 - Home Offset 0
6 - Offset S-R 9
7 - Offset R-R 10
2 - HORIZONTAL PROBE
0 - Tolerance 5
1 - Period 4
2 - Init. Period 16
3 - Ramp Steps 48
4 - Distance 600
5 - Position
#1 0
#2 161
#3 257
#4 334
#5 502
#6 140
#7 330
3 - VERTICAL PROBE
0 - Tolerance 10
1 - Final Freq. 2000
2 - Initial Freq. 400
3 - Ramp Steps 20
4 - Distance 1100
5 - Level Thresh. 250
4 - PUMP
0 - Withdraw 100
1 - Period 4
5 - TEMPERATURE
0 - Reaction Calefactor
0 - Prop. Gain 64
1 - Prop. Limit 1024
2 - Int. Gain 1
3 - Int. Limit 512
4 - Der. Gain 0
5 - Der. Limit 512
6 - P+I+D Shift -1
7 - Hardware 1
1 - Probe Calefactor
0 - Prop. Gain 32
1 - Prop. Limit 1024
2 - Int. Gain 1
3 - Int. Limit 512
4 - Der. Gain 8
5 - Der. Limit 512
6 - P+I+D Shift -1
7 - Hardware 1
6 - MISCELANYS
0 - Set Timer 364
66
1 - Show greater interrupt
2 - Delay MH1 2
1 - SENSOR SEEK
0 - REACTION TRAY
0 - Initialization
1 - Zero
0 - CCW
1 - CW
2 - Verification
0 - CCW
1 - CW
0 - Initialization
1 - Zero
0 - CCW
1 - CW
2 - Verification
0 - CCW
1 - CW
0 - Initialization
1 - Zero
2 - Verification
3 - VERTICAL PROBE
0 - Initialization
1 - Zero
2 - Verification
3 - Level
2 - COMMANDS SIMULATION
0 - REACTION TRAY
0 - To Cup XX Rn1XX
1 - Advance XX Cups Ra1XX
2 - Go Back XX Cups Rr1XX
3 - Advance « Cup RA1
4 - Go Back « Cup RR1
5 - Advance XXXX Steps Rf1XXXX
6 - Go Back XXXX Steps Rb1XXXX
7 - Vibration (Elong, Quant, Per) Rv1EEEQQQPP
0 - To Sample XX Ss1XX
1 - To Reagent XX Sr1XX
3 - Advance XXXX Steps Sf1XXXX
4 - Go Back XXXX Steps Sb1XXXX
5 - Vibration (Elong, Quant, Per) Sv1EEEQQQPP
1 - To Position #1 H11
8 - To Right XXX Steps Hr1XXX
9 - To Left XXX Steps Hl1XXX
A - Valve On XX time Hv1XX
3 - VERTICAL PROBE
0 - To Upper Sensor VU1
1 - To Lower Sensor VD1
2 - To Level Sensor + XX steps VN1XX
3 - Up XXX Steps Vu1XXX
4 - Down XXX Steps Vd1XXX
67
4 - PUMP
0 - Advance MMMM + NNNN Steps Ba1MMMMNNNN
1 - Go Back MMMM + NNNN Steps Br1MMMMNNNN
2 - Advance MMMM + NNNN - Withdraw BA1MMMMNNNN
3 - Friction RR / EE / Q Bm1RRRREEEEQQ
5 - INITIALIZATION
0 - REACTION TRAY IR1
1 - SAMPLE/REAGENT TRAY IS1
2 - HORIZONTAL PROBE IH1
3 - VERTICAL PROBE IV1
4 - PUMP IB1
6 - MULTIPLE COMMANDS
0 - Initialization MI1
1 - To Sample XX and Position #3 M11XX
2 - To Reagent XX and Position #4 o #5 M21XX
3 - To Cup XX and Position #1 M31XX
7 - Empty to XXX M71XXX
8 - Aspirate to XXX M81XXX
9 - Horiz HHH w/dir D; to Sample SS M91HHHDSS
B - Cup CC, Sample SS and Pump PPPP MB1PPPPSSCC
C - Hor to pos HHH & S/R to pos SSS MC1HHHSSS
Y - Reag XX, Pos 4; Level + YY ME1XXYY
N - Sample XX, Pos. #3; Level + YY MF1XXYY
DEL- Down XXX step; valve On YY time MH1XXXYY
. - Vert. up; Pos #2 & Pump Withdr. MK1
7 - CARRUSEL STATUS E11
8 - TEMPERATURE MODULES
0 - Initialize Reaction Temperature TR1I
1 - Set Reaction Temp. to XX§C TR1T000XX
2 - Disable Reaction Temperature TR1J
3 - Read Reaction Temp. (XX,YY§C) TR1t
4 - Initialize Probe Temperature TC1I
5 - Set Probe Temp. to XX§C TC1T000XX
6 - Disable Probe Temperature TC1J
7 - Read Probe Temp. (XX,YY§C) TC1t
9 - SET VOLATILE PARAMETERS

0 - REACTION TRAY
0 - Tolerance PR1t00XXX
1 - Period PR1p00XXX
2 - Distance PR1d0XXXX
3 - Offset PR1o00XXX
0 - Tolerance PS1t00XXX
1 - Period PS1p00XXX
2 - Distance PS1d0XXXX
3 - Offset PS1o00XXX
4 - Offset S-R PS1s00XXX
5 - Offset R-R2 PS1r00XXX
6 - Offset R-R1 ???
0 - Tolerance PH1t00XXX
1 - Period PH1p00XXX
2 - Distance PH1d0XXXX
3 - Position
#1 PH110XXXX
#2 PH120XXXX
#3 PH130XXXX
#4 PH140XXXX
#5 PH150XXXX
#6 PH160XXXX
#7 PH170XXXX
3 - VERTICAL PROBE
0 - Tolerance PV1t00XXX
1 - Frequency PV1f0XXXX
2 - Distance PV1d0XXXX
3 - Level Threshold PV1l0XXXX
68
4 - PUMP
1 - Period PB1p00XXX
A - ENABLE MOTORS
Y - Enable
0 - REACTION TRAY @e10
1 - SAMPLE/REAGENT TRAY @e11
2 - HORIZONTAL PROBE @e12
3 - VERTICAL PROBE @e13
4 - PUMP @e14
A - All @e1
N - Disable
0 - REACTION TRAY @d10
1 - SAMPLE/REAGENT TRAY @d11
2 - HORIZONTAL PROBE @d12
3 - VERTICAL PROBE @d13
4 - PUMP @d14
A - All @d1
3 - AUTOMATIC SEQUENCE
1 - WITH LEVEL DETECTION

2 - WITHOUT LEVEL DETECTION
3 - VERTICAL Test
0 - Short
1 - Long
4 - HORIZ and VERT Test
5 - TRAY Test
0 - REACTION TRAY
69
2.18 CALIBRATION AND ELECTRONIC CONTROL
2.19 CPU BOARD (M230X-P213).

• With the auxiliary board (M1401-P151) connected to J7 (COMU), press SWITCH
A (PB1) on M230X-P213, input value 4095 with numeric keyboard and press
Enter to adjust the maximum output of the DAC that controls the halogen lamp.
Connect a DVM to test lamp voltage and adjust 6 VDC with preset P1.
2.20 PREAMPLIFIERS (M85X-P160)

• SAMPLE CHANNEL PREAMPLIFIER
The values of the resistance and capacitors are:R1 = 470 Kohms
R2 = 470 Kohms
C1 = .047 Uf
• REFERENCE CHANNEL PREAMPLIFIER
The values are:
R1 = 3,92 Mohms
R2 = 3,92 Mohms
C1 = 4n7 Plate
2.20.1 ZERO ADJUST

With instrument OFF, disconnect J13 y J14 of COMU Interface M230X-P219.
Place a Jumper between ground and output (TP6-2 and TP6-3) of ADG408 (U6).
Turn instrument ON. With the maximum gain (15) eliminate offset by adjusting P3 to
read 0 mV at the output of operational AD711 (U4-6) - TP6-1.
Adjust P2 to obtain aprox. 5000 counts (Integration time = 10)
With instrument OFF, connect sample preamplifier to J14 and reference

preamplifier to J13 of COMU Interface.
Remove jumper placed on TP6.
Turn P1 and P3 clockwise in both preamplifiers (M85X-P160) to the end (GAIN
MAXIMUM)
Turn instrument ON, cover both preamplifiers with a black cloth. Select filter 0 and
adjust P2 with DVM in such way that the HIGH output (3J1) is 0 +/- 1 mV.
Check that LOW output (1J1) does not exceeds 0 VDC +/- 15 mV.
Verify that there is saturation at room light
2.21 PROBE ARM PCB (M230X-P222).

• Remove the jumper placed W1-3 and W1-4.
• Connect LM35CH simulator at W1-2, W1-4 and W1-6.
• Adjust calibration potentiometer to read 270 mV at TP1-1, adjust P1 (“0”) to read 0
mV at TP1-3.
• Adjust P2 (“256”) to read 4.809 V at PIN 5 of U29 on M230X-P216 - CARRU
Interface (“V ref”).
70
• Disconnect calibration potentiometer and set the jumper between W1-3 and W1-4.
LM35CH SIMULATOR +15

W1-2
10K
W1-4
1N4148 TP1-1
10K
W1-6
2.22 REACTION TRAY HEATER (M230X-P216)

• Remove J19 connector.
• Connect LM35CH simulator to J19.
• Adjust calibration potentiometer to read 240 mV at TP1-1, then adjust P2 (“0”) to
read 0 mV at TP1-3.
• Adjust P1 (“256”) to read 4,735 V at PIN 5 of U4 (“Vref”).
• Disconnect calibration potentiometer and connect the temperature sensor at J19.
2.23 PUMP CONTROLLER (M230X-P216)

The pump controller sets the chopping frequency to all the system.
• Oscillation frequency (TP7-1) ≈ 60KHz

• Voltage at TP6-1 = 300mV
• Initialization:
|¦------ 2 - COMMANDS SIMULATION
|------- 5 - INITIALIZATION
|------- 4 - PUMP
• Pump must rotate clockwise, when type "Ba1" command is executed.
2.24 VERTICAL CONTROLLER (M230X-P216)

• To perform initialization:
|¦----- 1 - SENSOR SEEK
|----- 3 - VERTICAL PROBE
|----- 0 - INITIALIZATION
• Verification sensor is searched for through commands:
|----- 2 - VERIFICATIÓN
• Distance between sensors is inputted:
|¦----- 0 - SET PARÁMETERS
|----- 4 - Distance: 1100
71
• To adjust parameters of vertical probe movement, lower probe arm to lower rest
position with the motors de-energized, then initialize. If fails to init. Increase the
tolerance setting. Repeat as necessary, tolerance must not exceed 15 steps.
|----- 0 - Tolerance: 15
2.25 HORIZONTAL CONTROLLER (M230X-P216)

• Initialization:
|----- 3 - HORIZONTAL PROBE
• Verification sensor is seeked:
When performing this operation, display shows the actual distance, in steps between
stop positions. Repeat initialization and verification 5 times and register steps.
Differences observed should not exceed 4 steps.
• Input steps between sensors, (maximum displacement):

|----- 4 - Distance: 600
2.26 SAMPEL/REAGENT TRAY CONTROLLER (M230X-P216)
• Initialization:
|----- 1 - SAMPLE/REAGENT TRAY
• Distance in steps depend on motor’s steps per revolution.
For 200s/r motors : Distance = 500
|----- 4 - Distance: 500
• Search for verification sensor (Inequality must be satisfied:
Distance – Tolerance - 10 ≤ Position ≤ Distance + Tolerance)
|----- 1 - CW
Tray must rotate clockwise.
72
• To verify tolerance, advance tray 48 sample positions. Repeat for 16, 32 and 0, at
least 5 times.
|¦----- 2 - COMMANDS SIMULATION
|----- 0 - TO SAMPLE XX
If error is found, increase tolerance and repeat:

|¦----- 0 - SET PARAMETERS
|----- 0 - Tolerance 10
2.27 REACTION TRAY CONTROLLER (M230X-P216)

• Initialization:
|----- 0 - REACTION TRAY
|----- 0 – INITIALIZATION
• Rotation steps depend on motor’s steps per revolution:

For 200s/r motors : Distance = 600
|----- 4 - Distance: 600
• Verification sensor seek must satisfy:

Distance – Tolerance - 10 ≤ Position ≤ Distance + Tolerance

|----- 1 – CW
Tray rotates anti-clockwise.
• Correct tolerance verification: advance tray 80 positions. Repeat 5 times.

|¦----- 2 - COMMANDS SIMULATION
|----- 1 - ADVANCE XX CUPS
If an error is encountered, increase the tolerance and repeat:

|----- 0 - Tolerance 10
73
2.28 AUXILIARY BOARD (M1401-P151) TO COMU CPU
• The test program is accessed setting SW1 of Dip-Switch in Off, SW2 to On and
the rest to Off.
• The following variables should by verified in COMU :
a) time_com: is the maximum time COMU waits from character to character
b) within a command packet from PC.
c) time_filtro: the delay COMU sets between two consecutive pulses to move
the filter wheel.
d) time_brazo: the delay COMU sets between two consecutive pulses when
moving the cuvette washer.
e) time_comMB: the delay COMU sets after CARRU sets the CTS signal in high
in the MB0 command once reaction tray is positioned and before performing a
reading.
f) vol_bomba: time the flushing pump operates, (only in the test program).
g) delay_valve: time the aspiration pumps operate.
h) vel_com: COMU serial ports to PC and to CARRU can be set to 9600 or
19200 Bauds.
i) vel_cavro: diluter velocity for use within the test program.
j) const_vel: conversion constant for diluter velocities for different models
supported.
k) pasos_comMA: steps diluter performs after reagent take-up.
l) lav_en: cuvette washer enable.
m) pasos_VU1: number of steps in slow movement in command VU1.
n) codigo_enable: bar code reader enable.
o) ISE_enable: ISE enable.
p) lamp_low: lamp value after power save command.
q) lamp_high: operation lamp value.
r) day: internal use of ISE routines.
s) month: internal use of ISE routines.
t) year: internal use of ISE routines.
To define these variables, key NO must be press, the display scrolls the following
table:
NOTE: Default values are mentioned below just for reference, for the
instrumentparameters, refer to the parameter sheet located on the instrument
cover.
FUNCTION DISPLAY DEFAULT

Time-out PC time_com 600
Delay photometer time_filtro 150
Delay washer time_brazo 70
Delay command MB0 time_comMB 400
Delay pump filling vol_bomba 25
Delay pump aspirating delay_valve 10
Baud rate vel_com 1:19200 bauds
Velocidad diluter vel_cavro 12
74
FUNCTION DISPLAY DEFAULT
Conv. Constant const_vel 270
Steps, command MA0 pasos_comMA 10
Washer enabled lav_en 1
Steps, command VU1 pasos_VU1 30
BCR enable codigo_enable 0
ISE enable ISE_enable 0
Lamp power save mode lamp_low 1000
Operation lamp value lamp_high 4000
Internal use of ISE routines day 15
Internal use of ISE routines month 3
Internal use of ISE routines year 02
• For the Baud Rate parameter to become active, reset COMU.
2.29 PHOTOMETER CONTROLLER (M230X-P219)

Values to be set are:
• Oscillation frequency (TP5-1) ≈ 60KHz
• To initialize, press key "1" in auxiliary board.
• To advance one filter position, strike "2" in auxiliary board.
2.30 DILUTER CONFIGURATION

• To initialize, strike ESC in auxiliary board.
2.31 +5VDC POWER SUPPLY

Use W1 jumper to adjust the output voltage of the M230X-P218.
2.32 INGRESO DE LAS POSICIONES HORIZONTALES

Horizontal positions for probe are inputted as follows:
0 – SET PARAMETERS
2 – HORIZONTAL PROBE
5 - Positions
#1 0
#2 161
#3 257
#4 334
#5 502
#6 140
#7 330
Position #1 must always be 0.
For the rest, a number of steps is inputted so that the probe stops as precisely as
possible, (better than ±1 mm.), at the center of the nominal positions.
2.33 PROBE VERTICAL MOVEMENT TEST

• Perform 10 movement sequences, [3-1] to test level sensing.
• Verify probe’s collision sensor operation by stopping while moving.
75
• Perform a movement sequence [3-2] of 50 cycles for each horizontal position to
verify that no steps are lost at each stop position. Ensure level detection at each
station. Repeatability must be within 5 steps.
2.34 FILTER WHEEL OPERATION TEST

• Recalibrate 10 times employing key 1. Verify wheel stops always in same position,
(Filter 0).
76
77
3 MECHANICAL, OPTICAL and HYDRAULICAL SETUP
4.1 INSTRUMENT TEARDOWN FOR SERVICE

(Only to be performed by qualified personnel).
WARNING: Before attempting any instrument teardown, unplug instrument

from mains supply.
Removing the rear panel may expose hazardous life connections. Only trained
personnel should perform operations with covers removed and instrument
plugged to mains.
To have free access to all components of the instrument in order to service, the
following steps must be followed carefully (see pictures at the end of this manual):
1. Unplug instrument from mains and disconnect computer. Remove drain and wash
solution reservoirs to prevent accidental spillage.
2. Lift protective cover to access the probe arm. Remove probe arm cover and then
the probe to avoid damaging it.
3. Remove the rear panel and unplug fan and serial port connectors from the
instrument.
4. Remove the screws that fix lower base plate to the deck, if the instrument has
lateral side covers, remove them first to access the screws.
5. If the instrument has a plastic front panel, remove it and then remove the metal
shield to access to funnel fitting, next, with great care, lift the deck slightly to free
it.
6. Pull the instrument cabinet up gently.
To re-assemble, proceed in inverse order. Remember to replace and tighten ALL the
screws properly to restore electromagnetic shielding action and adequate protective
grounding.
4.2 OPTO-MECHANICAL ADJUSTMENTS
After a major repair involving the removal of mechanical or optical components, it will
be necessary to perform a calibration to restore instrument to original settings. For
better comprehension, refer to drawings in the last section. The checklist to perform
calibration follows, ( refer to drawings in last section):
1. Verify belt tension on every unit. Do not over-tension belts, just eliminate slack.
78
2. Re-tighten all screws.
3. Verify no interference occur between optical sensors and moving mechanical
parts.
4. Make sure cables and tubing do not interfere with moving parts.
5. Adjust the position of the clamp hub on the robot elevation shaft so that the probe
reaches the bottom of the sample tubes in its lowest operational position. Probe
vertical movement should reach a stop between 0.1 and 0.5 mm before impacting
bottom of sample tubes.
6. Adjust horizontal play of probe arm to less than 0.5mm measured at the probe’s
location. The guide fork on the robot shaft has a set screw and a lock nut to
remove play. Do not eliminate play totally, or friction will impede normal operation.
7. Reset all movements of the instrument. In photometer, set filter #5. Watch the
green light beam in the reaction cuvette. The beam should pass through the
center of the cup ±0.3 mm. Adjust reaction tray offset until beam is centered in cup
number 44. (Refer to electronic section for instructions on how to adjust the
offset). Use a cuvette strip with ground faces to aid the observation of the light
beam. Use a fine emery paper to sand the cuvette faces.
8. Adjust probe arm length in order to center probe in cuvette cup #1. Lower probe a
sufficient number of steps into the cuvette to aid centering within ± .5 mm.
9. With robot horizontal position #1 selected, lock robot arm to the elevation shaft
when probe is centered with reaction cuvette ± .5 mm. Lower the probe until it
enters the cuvette to aid adjustment. Horizontal positions 2 to 7 of the probe arm
and reagent and sample tray’s offset are adjusted by inputting a convenient
number of steps to move the arm or tray to the selected position and then saving
the settings, (refer to electronics section). This last operation should be performed
with the deck plate in place and does not require access to mechanical or
electronic components.
10. Adjust the position of the wash head so the drying block enters and exits the
cuvette without interference. This adjustment is performed unlocking screws fixing
the wash head support arm to its elevation bracket. Lower wash head into cuvette
for self-centering action and then tighten screws gently. Operate the wash module
by hand or with the auxiliary keyboard and verify the drying block enters and exits
the cuvette properly. Re-adjust as necessary. Tighten screws.
11. If wash head vertical position must be re-calibrated, unlock the adjustment screw
on the crank-wheel support frame and lower or elevate the frame plate as
required.
12. The wash volume dispensed during wash sequence is adjusted varying pump time
in parameters menu. Typical value is 20. It should be adjusted so that 0.5±0.2 ml
are dispensed into each cup undergoing washing process.
79
80
4 FILES AND DATABASES INCLUDED IN THE SYSTEM
4.1 FILES
File Description
Main.exe Main module
Desil1.isu, etc. Uninstall information
Errors.log File containing errors (opens with Worpad)
Backup.exe Program of storage and backup of main and databases
About. Txt Text file containing description displayed in Help "About"
Priv (directory) Temporary storage of databases and data.
Bu_X.Zip Compressed file containing bakup of day x ( x=1, Monday, etc.)
Analyzer.wav Sound file used for alarm when sound board/speakers are used
Release.rtf Info shown in "What is new" in Help option
Readme.txt Information not contained in last manual version
Data Sub-directory with databases
Help Help files
4.2 DATABASES
Analyzer utilizes Borland Paradox database configuration, version 5 (compatible with

Paradox Corel version 8)
The following databases are included in the system. Samples and historic include two
databases each, one for sample description and the other for analysis belonging to
the sample. Databases are located in sub-directory Data.
Database FILES
Methods Metodos.db Metodos.px Metodos.val Metodos.mb
Methods in Usdmeth- Usdmeth.px Usdmeth.val
Use s.db
Samples Muestras.db Muestras.px Muestras.valMuestras.xg0 Muestras.yg0
Análisis.db Analisis.px Analisis.valAnalisis.x02 Analisis.y02
Historic Historic.db Historic.px Historic.valHistoric.xg0 Historic.yg0
Historic(sple) Anahist.db Anahist.px Anahist.val Anahist.x02 Anahist.y02
Interferences Interfer.db Interfer.px Interfer.valInterfer.x01 Interfer.y01
Interfer.x02 Interfer.y02
Standards Detperf.db Detperf.px Detperf.val Detperf.x02 Detperf.y02
Perfiles.db Perfiles.px Perfiles.val Perfiles.xg0 Perfiles.yg0
Trays in Bandejas.db Bandejas.px Bandejas.val Detband.x03 Detband.y03
memory Detband.db Detband.px Detband.val
Filters Filtros.db Filtros.px
Calibrations Calibs.db Calibs.px
Volumes Totvols.db Totvols.px
Priorities priors.db priors.px priors.val Priors.xg0 priors.yg0
Times Vasos.db Vasos.px Vasos.val Vasos.xg0 Vaso.yg0
81
Times (Hist.)VasosH.db VasosH.val
Parameters Params.db Params.mb
Translator Traduct.db Traduct.px Traduct.tv Traduct.xg0 Traduct.yg0
Print queue Prana.db Prana.px Prana.val Prana.x02 Prana.y02
Toprint.db
Windows po- Desktop.db Desktop.px Desktop.val
sitions and Columnas.d Columnas.px
sizes b
Messages Logs.db Logs.val
Math.func- Funcion.db Funcion.px
tions
Samples in Sueros.db Sueros.px Sueros.val Sueros.x02 Sueros.y02
tray
Reagents in Reactivs.db Reactivs.px Reactivs.val Reactivs.x02 Reactivs.y02
tray
Calibration Testigos.db Testigos.px Testigos.val
curve
(ELISA)
Anal. type Tipos.db tipos.px
Last statis- Statis.db
tics
Simulation Simul.db
queue
Samplex to Expmues.db Expmues.px
expire Expana.db Expana.px
Any method includes:
Method to Name.db Name.px Name.val Name.db

import
82
5 MAINTENANCE
5.1 SELECTING WORKING TEMPERATURE
Temperature is selected either to room, 30ºC or 37ºC from the Temperature

Parameter located in Technical Parameters.
5.2 REPLACEMENT AND CONTROL OF WASH SOLUTION
Analyzer washes the sampling probe between sample take-up, requiring

approximately 1 ml of solution for each test performed. The distilled water is pumped
up from a reservoir and is disposed to the drainage container, both provided with the
instrument. Both reservoirs have electro-pneumatic level sensors.
Washing bottle EMPTY. Will continue?
This message appears after initialization, but does not impede instrument operation.
As enough distilled water is still present, the run can be completed before refilling the
reservoir. If no water is added, message reappears before next run.
When the drainage reservoir is full, a message is shown:
Drain bottle FULL. Will continue?
The preceding paragraph applies to this case as well.
5.3 PUMP TUBING AND SYRINGE REPLACEMENT.

The pump tubing has a pre-fixed number of cycles for replacement. When that
number is surpassed, in the operating window a message will be shown:
83
This message means that operation will not necessarily be interrupted. At the earliest
opportunity the replacement must be done.
After replacement, select Parameters, then Cycles and press the button 0.
This resets the counter. Otherwise, the warning message will continue being shown.
The same procedure is valid for syringe replacement.
The cuvette washing system includes two pumps: washer and aspirator. These
pumps must be replaced when the corresponding number of cycles is accomplished.
5.4 LAMP REPLACEMENT
The user can easily perform lamp replacement by following the instructions:
1. Turn off and unplug instrument from mains. Remove right lateral cover by removing
the screw that hold it in place. (See Figure 1)
2. The lamp will be visible when cover is removed (see drawing at the end) and
protecting grid is removed by 4 cross screws. Remove the two screws that fix lamp
holder to photometer body.
Unplug lamp cable from connecting plug, (press small lever at side of plug to
disengage).
3. Install a new lamp holder with pre-focused lamp in. Reset screws and tighten. Plug
in lamp connector. Do not touch lamp bulb. If touched accidentally, clean with
lintless cloth or tissue paper and alcohol.
4. If lamp bulb has a protective envelope, remove it. Reinstall protecting grid, the
lateral cover and fixing screws.
5. Start instrument in the order mentioned in Startup. Perform a calibration cycle. To

do so, enter Movements menu and then select Calibrate.
6. Open Inspect and then the Filters window. Compare obtained readings with
previous calibrations. Take note of gain settings for each filter. If values differ from
previous in 3 or more gain steps, adjust must be performed. Observe in the Status
column if any Gain is too high or too low.
5.5 SAMPLE PROBE CARE
The sampling probe is a delicate part of the instrument. Precision of results is in a

good measure dependent on how it is maintained.
Probe tip must be kept clean.
A cleaning cycle must be performed when indicated. If proteic deposits are seen on
the tip, remove gently with a tissue paper.
84
NEVER USE ABRASIVE MATERIAL: THE DELICATE COATING SURFACE
WILL BE DAMAGED.
If the tube tip is defective, remove cover of probe arm, loosen setscrew that retains
the needle and pull it up. Install new probe. Tighten setscrew connector fitting and
cable and repeat procedure outlined in 5.5.1.
5.5.1 CALIBRATION PROCEDURE FOR PROBE ARM (ONLY IF REQUIRED)
1. Insert an empty sample vial in position 1 in the sample tray. With Manual
movements Send probe arm to position Sample 1 and then Down. Move vial
vertically and establish its vertical play.
Loosen the split hub clamp in the elevation column, under probe arm and lower it an
amount equal to vertical play of vial, tighten in that position.
Loosen 2 hexagonal socket screws that link arm to elevation column, lower probe arm
until it rests on hub clamp. Tighten screws while making sure probe arm is centered in
vial.
2. Measure at least one sample with distillate water instead of sample and reagent.
Verify for different sample positions that probe is centered in vial.
5.6 HYDRAULIC CIRCUIT CONTROL AND MAINTENANCE.
Correct hydraulic system operation is essential to obtain consistent and reproducible

results.
Malfunction symptoms are:

1. Erratic readings and low reproducibility.
2. Volume dispersion in reaction cups for a same method.
3. Droplets or drop formation on probe tip after each wash cycle.
4. Leaks in the system produced by defective connections or capillary
obstruction by kinks or solids.
To verify, perform the Dilution test.

Most common problems encountered in hydraulic system are:
1. Pump tubing wear introducing efficiency loss and eventual leaks. Replace
tubing.
2. Hydraulic system obstruction. Particles present in distilled water may clog
filter in pump connector. This usually happens when metal
distillers are used.
3. Kinks in probe arm heater tubing or defective connections.
4. Sampling tube in probe clogged by solids from samples or
85
reagent take-up.
IMPORTANT: Clean peristaltic pump filter and replace pump tubing on a regular
basis, determined by experience.
To clean hydraulic system, proceed as follows:

1. Inspect malfunction by sections, start with pump filter, and then verify
pump operation disconnecting tube from syringe in diluter and verify dispensed
volume. (Refer to drawings at the end of user manual to disassemble
connectors).
2. Next disconnect at heater entrance in probe arm and verify dispensed
volume to detect clogging.
2. Disconnect heater in probe arm from sampling capillary and repeat test.
3. If probe tube is clogged, flush with the aid of a syringe or replace.
4. Replace or clean clogged filter, if necessary.
NORMALLY OBSTRUCTION WILL DISAPPEAR BY PUMP FLUSH ACTION

WHEN DISCONNECTING AND DISPENSING AT THE MENTIONED POINTS.
5.7 CUVETTE WASHER MAINTENANCE
DIAGNOSTIC
1. Perform washer test.
2. Wash volume in stations two and three must be between 400 and 700
microliters. If not, modify volume in the Functional Parameters. If volume
required falls off the range 10 to 90, check for clogging in the system or modify
needle valve setting.
3. If volume difference between stations 2 and 3 is greater than 20%, cleaning of
hoses is required.
4. If liquid remains un-aspired in stations 1, 2 and 3, some of the hoses are
clogged. Stations 2 and 3 correspond to the same pump. If liquid is present
either in 2 but not in 3 or in 3 but not in 2, the corresponding hose is clogged. If
liquid is present in both, either pump is faulty or the common tubing is clogged.
5. Station 1 has its own aspiration pump. If liquid is remnant, check for clogged
tubing or hose or faulty pump.
IMPORTANT: Do not modify needle valve setting unless positively sure that
there is not clogging in the system or faulty pump.
5.7.1 VOLUME ADJUST

6. Select Test tab in Manual Movements. Initialize washer.
7. Disable Reaction Tray by turning it OFF. This operation is performed in the tab
of Globals.
8. Dispense wash solution by pressing the Dispense button in the Test tab.
86
9. Move reaction tray to different positions and repeat dispensing. Remove
cuvette and observe delivered volume.
10. If necessary adjust volume parameter in Factory, Cup washer, Volume.
11. Select Test tab in Manual Movements. Initialization button sends drying
module up and "Descend" button send it down. Check for smooth movement in
different positions in reaction tray.
5.7.2 DRYING BLOCK REPLACEMENT.

Drying block should be replaced only if symptoms of poor drying capacity are
detected.
Colored block (usually yellow from Creatinine reagent) is not necessarily a reason to
change it.
If cross-contamination is observed, first check if drying is good. Poor drying power
can be corrected by drying block replacement. For calibration, see Section 5.7.2
Drying block can be replaced by firmly pulling it down and inserting new one in tubing.
If operation is difficult, take reaction tray out by removing four screws that fix it, insert
block and re-install reaction tray. Position is unique.
5.8 PHOTOMETER AND FILTER CLEANING
1. Verify that the instrument is turned off and unplugged.

2. Remove right lateral cover as explained in 5.4
3. Provide adequate external illumination for the following operations.
4. Localize photometer and filter wheel.
5. Use cotton swabs to remove dirt from filters surface. Rub gently until opalescence
is removed.
NOTE: Filter Nr.0 is an opaque dummy, and requires no cleaning.
6. After cleaning is complete, replace cover, turn on the instrument and allow 15
minutes warm-up.
7. Perform a “Calibration”. Normally, energy increases by 15% after clean up.
5.9 DETECTOR LENS CLEANING
Detector is accessed within the reaction chamber. Reaction tray must be removed for
cleaning process.
1. Unplug instrument from mains.

2. Remove 4 cross-recessed screws from tray. Remove tray with caution, it is fragile
and should be handled with care.
3. With a lintless moisten cloth clean reaction chamber if spillages are visible.
4. Clean discharge funnel with Sodium Chloride solution and soak with distilled water.
5. Clean detector lens in slit with a cotton swab.
6. Replace reaction tray and screws.
87
5.10 PREVENTIVE MAINTENANCE PROGRAM
Scope: To familiarize operator with maintenance program of the analyzer, as

recommended by manufacturer.
Applies to all instruments manufactured after 05/01/94, (see serial number).
Some items are not present in instruments manufactured prior to 09/01/95, and
should be incorporated at a latter time.
Requirements: The Maintenance program includes the following aspects:
*Daily care.
*Weekly care.
*Quarterly care.
*Maintenance as required.
Operator will also find in this document:
*Cleaning and Adjustment procedures;

*Controls and Diagnostics.
*Quarterly Maintenance Form.
5.10.1 RECOMMENDED DAILY CARE.
Recommended operations should be performed at the start of every shift or on

demand.
1. Purge hydraulic system by selecting Movements, Cleaning, Fill with pump.
During process look for:
*Presence of bubbles or air gaps in system.
Air gaps and bubbles should be flushed, if present, during the fill-up operation. It is
normal to find some bubbles in the peristaltic pump tubing. Repeat process if
necessary.
In case new bubbles generate in the process, determine the origin:
Come from reservoir?

Generate in pump connectors?
Generate in syringe connectors?
Are they visible only in probe tip?
To solve problems, see Appendix, 6.5: Control and maintenance of hydraulic system.
*Leakage in peristaltic pump.

88
Replace pump tubing even if cycling time is not reached.
* Constant and uniform flow from probe tip.
This indicates hydraulic system is operating normally.
* No droplets hanging on probe tip.
When system operates normally, no droplets should be present on outer part of tip. If
tip is dirty, droplets will adhere to external surface. If obstructions are present in the
system, flow will be intermittent and drops will continue to fall after pump has stopped,
and eventually, one will remain hanging from the tip.
When system operates normally, flow will stop instantaneously when pump stops.
5.10.2 INSPECTION AND CLEANING OF PROBE.
Important: Accomplish all automatic-cleaning cycles required by the instrument.
*Remove solids from tip and electrodes with a cotton swab embedded in soak
solution. Dry with tissue paper, eliminate lint.
5.10.3 WEEKLY CARE RECOMMENDATIONS.
* Repeat daily maintenance procedures.
* Empty and clean drainage reservoir, including stopper.
* Clean drain funnel in wash station. Use Hypochlorite solution and soak
with water.
* Clean reagent/sample tray by submersing it in mild detergent and water.

Rinse with tap water and let dry. Do not heat to dry. If desired, dry with a
towel or lintless cloth.
* Clean instrument board with a moisten cloth. Do not use organic solvents
or acids to clean.
* Replace distilled water and eliminate leftover.
*Execute a Washing cycle from the Cleaning menu.
89
5.10.4 QUARTERLY MAINTENANCE RECOMMENDATIONS.
*Optical Filters cleaning.

Before cleaning, perform a Calibration. Keep a printout for data comparison.
*Cleaning of distilled water reservoir. Clean reservoir, stopper and electrodes

with Sodium Hypochlorite solution. Rinse with abundant tap water and then with
distilled water. Replace distilled water and perform at least three fill-up cycles.
*Distilled water filter cleaning or replacement. Clean mesh filter or replace.

(See drawings at end of manual).
5.10.5 MAINTENANCE ON DEMAND.
Must be performed when instrument indicates the need of corrective action, or when
operation anomalies are encountered, relative to maintenance:
*Hydraulic malfunction: droplet appearance on probe tip or bubbles in system.

Proceed as in section 5.10.1
*Message indicating pump tubing replacement. Replace tube and confirm with
YES in the corresponding display.
* Message indicating syringe replacement.

Replace syringe and enter YES on display. If syringe is replaced outside
corresponding menu, the syringe counter should be reset to zero. Proceed as
for tube replacement in pump, resetting the second displayed number.
90
91
6 TROUBLESHOOTING
Problems can be classified into 3 major groups:
1. Operation malfunctions with visual, acoustic or printed warnings.
2. Visible faults or problems.
3. Measurement inconsistencies, (for example: GOT method with high dispersion).
Definitions:
DM: Daily maintenance procedures.
WM: Weekly maintenance procedures.
QM: Quarterly maintenance procedures.
VT: Validation test.
6.1 OPERATION MALFUNCTION WITH WARNING

Sometimes, an error is displayed in more than one way. Printed errors correspond to
anomalous conditions found in samples and/or reagents and in no case stop the run.
They are only displayed in the error File. To access it, use Microsoft WordPad:
WordPad error file
The error file keeps the last 6 operations before the error occurred. It also contains all
start-up and instrument shutdown information.
Errors shown in the display can be automatically overridden without halting the run. In
Technical Parameters, the “Command Repeat” indicates the number of times an
order is repeated before system is halted. The default value is 3.
In some occasions, errors may be so serious as to force the operator to abort the run
and take corrective actions.
When the error does not affect the Reaction Tray and the photometric reading, only
dilutions will be stopped. A message “Stop Dispensing.” will be displayed. By
pressing the Resume button, dilutions will continue.
92
When a warning window opens:
Click to continue
Click to stop
sound warning
6.2 VISIBLE FAULTS

1. Drop formation on probe tip after dispensing.
2. Drop formation on probe tip after wash cycle.
3. Abnormal noises.
6.2.1 DROP FORMATION ON PROBE TIP AFTER DISPENSING.

Symptom Corrective Action
Drops on probe tip Verify hydraulic system in accordance to
user’s manual.
Clean probe tip by submerging in
Solution 1 for 5 minutes.
Review “Air Volume” in Technical
Parameters. Increase air volume only if
preceding actions have been taken and
93
problem persists.
6.2.2 DROP FORMATION AFTER WASH CYCLE.

Drops on tip after wash cycle. Verify hydraulic system for leaks or
obstructions.
6.2.3 ABNORMAL NOISES.

Abnormal noises. Defective fans.
Moving parts blocked or frozen
6.2.4 INACCURATE TEMPERATURE READINGS.

In “coordinates” temperature in reaction Room temperature too high, (should
tray is too high. (Do not be concerned always be at least 4°C lower than
about arm probe temperature) selected working temperature).
Example: For 37°C incubation
temperature, Room temperature should
not exceed 33°C.
For 30°C incubation temperature,
ambient must be lower than 26°C.
In “coordinates” temperature in reaction Room temperature excessively low.
tray is too low. (Do not be concerned Verify instrument operating range, and
about arm probe temperature) adequate the room temperature.
6.2.5 AUTOMATIC CUVETTE WASHER MALFUNCTIONING.

At the end of wash cycle, tiny water .Verify that all pumps are working.
droplets are in the cuvette walls Verify that no tubings are clogged
Replace drying block
Calibrate washer unit position.
High cross-contamination Identify cross-contaminants and set
methods in the Table of interferences
Increase the wash volume
Increase the number of wash cycles
6.3 INCONSISTENT RESULTS

1. All methods.
2. Only Colorimetric methods.
3. Fast Kinetics: all or some.
4. Only 2-point Kinetics.
94
5. Inconsistent automatic repetition values.
6. Coagulation.
When these problems occur, proceed in the following sequence to solve:
1. Verify Main power supply and ground connections. Measure voltage of ground
connection referred to neutral connector.
2. Use new reaction cuvettes.
3. Comply with Daily Maintenance Routines.
4. Perform a Validation Test VT to detect a module failure. (See Chapter 7)
6.3.1 ALL METHODS

Symptom: Erratic readings.
Possible Cause Validation Test Corrective Action
Unstable lamp. Energy Replace lamp and perform
Noise CALIBRATION.
Unstable photometer Noise Replace lamp and perform
readings. Photometric stability CALIBRATION.
Low signal to noise ratio. Energy Calibrate.
Noise Replace lamp and calibrate.
Perform quarterly
maintenance.
Hydraulic system obstruction. - Perform daily maintenance.
Inspect hydraulic system
Hydraulic system leaks. - Perform daily maintenance.
Verify hydraulic system.
Sample/reagent tray Photometric stability Perform daily maintenance.
positioning error. Dilution
Filter wheel position error. Photometric stability Check sensor.
Main power fluctuations. -- Verify. Use an Uninterrupted
Power Supply unit if
necessary.
Poor reaction cuvettes. Cuvettes Replace with new reaction
cuvettes.
Cuvettes are dirty or wet after Compare with results with
automatic wash. new cuvettes.
Take actions described in
6.2.5
6.3.2 COLORIMETRIC METHODS (ONE OR MORE)

Symptom: High dispersion of results.
Possible cause Validation Test Corrective Action
Low energy in filter (#). Energy Perform CALIBRATION.
If condition persists, change
95
lamp and calibrate.
Perform QM.
Insufficient or defective Energy Centrifuge sample for a
sample centrifugation. longer period of time and with
a greater number of
revolutions than for manual
methods.
Symptom: Normal values, having low dispersion, are too high or too low.
Defective standard. - Compare calculated factor
with previous factors stored in
the file. Perform calibration for
that method. If problem
persists, replace standard.
Contaminated standard. - Same as above.
Standard cross- - Change programmed order
contaminated. for standards.
Change job mode (batch
mode to profile mode or vice
versa).
Method’s linear range Differences between
exceeded. readings and
automatic
repetitions
Excessive sample volume. -
Stray light. Stray light
6.3.3 SYMPTOM: LOW LINEAR RANGE.
Excessive sample volume. -

Poor reagent condition. - Change reagent. Repeat
readings and compare.
6.3.4 FAST KINETICS

All Fast kinetics.
Symptom: Erratic readings (High dispersion or low linearity).
Possible Cause Validation Tests Corrective Action
Incubation time too short. -

96
Initial absorbance too high in - Verify reagent preparation.
decreasing kinetics. Replace if necessary.
Low energy in filter used. Energy Perform a Calibration. If
problem persists, replace
lamp and calibrate.
Too high electronic noise. Noise
Low precision reaction tray Cuvettes Perform QM.
positioning. Photometric stability
Erroneous positioning of Cuvettes Perform QM.
reaction tray. Photometric stability
Bad reaction cuvettes. Cuvettes Use new reaction cuvettes.
Reagent in bad condition. - Replace reagent. Compare
results, if possible program
simultaneously.
Defective lamp. Noise Replace lamp and calibrate.
Photometric stability
Lamp close to burnout. Noise Replace lamp and calibrate.
Symptom: Normal values too high.

Test Failed
Number
Electronic noise. Noise
Low available energy. Cuvetes Calibrate. If problem persists,
replace lamp and calibrate.
Lamp close to burnout. Noise Replace lamp and calibrate.

Symptom: Normal and pathological values too high.

Test Failed
Number
Incubation temperature too Movements
high.
Incorrect incubation - Verify temperature setting and
temperature setting. application.
Error in factor. - Verify if factor is correct for
selected temperature setting.
Symptom: Normal and pathological values too low.

Test Failed
Number
97
Incubation temperature too Movements
low.
Incorrect incubation - Verify if factor is correct for
temperature setting. selected temperature setting.
Error in Factor. - Verify if factor is correct for
selected temperature setting.
Some Fast Kinetics

Symptom: Erratic values.
Test Failed
Number
Low energy with filter used. Energy Calibrate. If problem persists,
change lamp and calibrate.
Incorrect wavelength setting. -- Review application.

Sample volume too low. -- Use adequate sample
volume.
Defective centrifugation. -- Use longer times and higher
speed than for manual
methods.
Too high initial absorbance -- Replace reagent. Compare
for decreasing kinetics. results.
Too low initial absorbance for -- Replace reagent. Compare
increasing kinetics. results.
Symptom: Normal values too high.

Test Failed
Number
Low signal to noise ratio for -- Calibrate. If problem persists,
the selected filter. replace lamp and calibrate.
Factor error for selected --
temperature and volumes.
Symptom: High or low values for all the range.

Test Failed
Number
Factor error for selected -
temperature and volumes.
6.3.5 2-POINT KINETICS

Symptom: Erratic readings.
98
Test fail Nr.
Absorbance of standard too -- Review application.
low.
Too high initial consumption -- Verify with data provided by
rate. reagent manufacturer.
Replace reagent.
Poor reagent condition. Replace reagent and
compare results.
Low signal to noise ratio for Calibrate. If problem persists,
selected filter. replace lamp and recalibrate.
Low sample volume. Review application.

Elapsed time too short. Review application.
Symptom: All normal and pathological values high or low.

Test Failed
Number
Factor or standard error. - Verify controls and method
used.
6.3.6 INCONSISTENT VALUES IN AUTOMATIC REPETITION OR DILUTION

Symptom: Colorimetric and kinetic reactions are not linear.
Test fail Nr.
Sample volume too high. - Review application.
Verify reagent’s linear limit.
Reagent in bad condition. - Replace reagent and
compare.
Symptom: Non-linear 2-point method.

Test Failed
Number
Cannot be repeated because -
volume/absorbance ratio is
not linear.
6.3.7 COAGULATION
Symptom: Erratic times.
Test Failed
Number
99
Poor reagent condition. - Replace reagent.
Reagent insufficiently mixed. - Mix reagents gently before
use. If many tests are
programmed, mix reagent
every 15 minutes.
Symptom: Coagulation times too short in normal samples.

Test Failed
Number
Incorrect wavelength setting. - Review method
Error in absorbance threshold - Review method
setting.
Symptom: Coagulation time excessive, or lack of coagulation.

Test Failed
Number
Threshold too high. -
Poor reagent condition. - Replace reagent.
100
6.4 MESSAGES AND WARNINGS
6.4.1 MESSAGES WHILE NOT OPERATING INSTRUMENT

Self-explanatory messages are not included in the present listing.
Message Cause Action
Incorrect Serial Number Introduce correct
Serial Number
Analysis to be performed. Attempt to edit a method Do not modify
in use. method still in use.
Change reaction cuvettes. All 80 cuvettes are used. Replace reaction
cuvettes.
Reaction tray not empty. Warning on the existence No action to be
Continue? of used cuvettes. System taken.
will use only clean
cuvettes.
Temperature is out of range. Not enough equilibration Abort startup and
Continue? time wait 5 minutes.
Replace syringe. Continue? Preset limit is surpassed. Replace at the
earliest opportunity.
Replace pump tubing. Preset limit is surpassed. Replace at the
Continue? earliest opportunity.
Replace wash pump. Preset limit is surpassed. Replace at the

Continue? earliest opportunity.
Place solutions in pos. 47 When testing is finished, Perform wash.
and 48. place solutions 1 and 2 in
positions 47 and 48.
Clear reaction cuvettes? When Clear button is Confirms cuvette
pressed. blanking.
Error: Sample already used. Attempt to load a sample Load any sample
already in sample tray. only once.
Error: Cannot put second No room in tray for Remove reagents
reagent. second reagent. not in use.
Error: Reagent already used. Attempt to load a reagent Load any reagent
already in tray. only once.
Error: system in automatic Attempt to blank or wash Blank samples with
mode. cuvettes while sample system stopped.
reading is still in
progress.
Field ‘XXXX’ must have a Some data is missing. Check for missing
value. values.
(*)
End of sample processing. Automatic mode is None.
ended.
101
Message Cause Action
Key violation. Invalid or repeated value. Check data and
correct.
Invalid variant conversion Attempt to convert a null Calibrate

type. character into a number. instrument.
When present dilution ends, Dilution has been When probe is in
load samples in tray and stopped. standby place
press Resume. samples in tray and
press Resume.
Nothing to be transferred. No data ready to be None.
stored in historic file.
There is no wash solution in One or both wash Replace required
position 47 or 48. solutions are missing. wash solution.
Reaction still in progress. Attempt to edit a method None.
still in use.
Repeated test ID. Only one test ID can be
stored with a given
name.
XX transferred samples Data transferred to None.
YY transferred analysis. historic file.
102
6.4.2 RUN-TIME ERRORS AND MESSAGES
Code Error Action Generator bit Origin Possible solution
and frequency
level
1 Probe wet. Dry it. Stop ST1-7 (E11) With resistive sensor, drop Dry electrodes. With capacitive probe,
between electrodes. With warm it to dry. Verify for leaks in upper
capacitive sensor, wet inner connector. If leak persists, replace probe
core
2 Dirty probe. Clean tip Stop ST1-7 (E11)
7 Error in Washer Repeat ST1-6 (E00) Manifold stacked at the bottom. Align drying block. Verify its size and
and Abort Manifold without energy shape. Check motor, connectors and
power supply
8 Error in Washer Repeat ST1-6 (E00) Manifold stacked at the bottom. Align drying block. Verify its size and
and Abort Manifold without energy shape. Check motor, connectors and
power supply
9 Error in diluter. Repeat ST1-0 (E22) Errors 52, 53 ó 54
and Abort
10 Error in level sensor

11 Waste flooded Stop ST1-7 In old models verify that waste funnel is not
flooded
12 Probe impact. Check Tip and Stop ST1-1, ST1-4, Stopcock in reagent or sample. Check and uncap reagents and samples.
ST1-5 (E11) Stacked movement Check for mechanical obstructions. Verify
arm led function
14 Error in Sample Tray Repeat ST1-3 (E11) Dirty sensor. Check sensor. Clean, if necessary. Check
and Abort Sensor touches lid. belt tension. Check for mechanical
Defective sensor. malfunctioning. Check power supply. With
Power supply motors off, check if motion is smooth.
15 Error in Reaction Tray Repeat ST1-2 (E11) Dirty sensor. Check sensor. Clean, if necessary. Check
and Abort Sensor touches lid. belt tension. Check for mechanical
Defective sensor. malfunctioning. Check power supply. With
Power supply motors off, check if motion is smooth.
and frequency
level
16 Error in pump Message ST1-6 (E11)
17 Error in Horizontal Repeat ST1-4 (E11) Sensor position1, dirty or Check sensors. Clean, if necessary. Check
and Abort defective. Mechanical for mechanical malfunctioning. Check
problem in arm power supply. With motors off, check if
motion is smooth.
18 Error in Vertical Repeat ST1-5 (E11) Upper or lower sensor Check sensors. Clean, if necessary. Check
and Abort defective. Mechanical problem. belt and gears. Check for mechanical
When level must be detected: malfunctioning. Check power supply. With
a) Stop at lower sensor: motors off, check if motion is smooth.
Reagent or sample missing.
b) Different number of steps:
vertical movement problem
20 Cover sensor error Message ST0-3 Sensor dirty, blocked or
deteriorated
21 Module busy Repeat ST0-2 Overloaded computer Eliminate unnecessary programs. Leave at
least 50 MB free memory. Remove all
screen savers and power savers
22 Module inoperative Abort Overloaded computer Eliminate unnecessary programs. Leave at
least 50 MB free memory. Remove all
screen savers and power savers
23 Time Out Abort No answer from Instrument off. Instrument is not Check connections, parameters and serial
analyzer connected to PC. ports
Error in communication
parameters. Defective Serial
Port, either in computer or
instrument.
30 CTS Time Out Abort CTS line not Instrument off. Instrument is not Check connections, parameters and serial
enabled by connected to PC. ports
analyzer Error in communication
parameters. Defective Serial
Port, either in computer or
instrument.
31 Error in temperature SPI Message Temperature External noise halted SPI Turn instrument off and re-start operation
controller temperature control
and frequency
level
32 Warning: Low free disk Message Less than free
capacity 50 Mb in disk
34 Sample channel low on filter Message In calibration, Defective Filter Check filter.
gain 31 and less Check lamp voltage.
than 25000 Check filter wheel malfunctioning
counts, sample
channel
35 Sample channel saturated on Message In calibration, Defective Filter Check filter.
filter gain 0 and more Check lamp voltage.
counts, sample
channel
36 Reference channel low on Message In calibration, Defective Filter Check filter.

filter gain 31 and less Check lamp voltage.
counts,
reference
channel
37 Reference channel saturated Message In calibration, Defective Filter Check filter.
on filter gain 0 and more Check lamp voltage.
counts,
reference
channel
38 Error in 0%T. When run ends, Message Read frequency Zero setting or calibration, Calibrate. If persists, check noise stability
recalibrate. 50 counts less changed and power supplies
than zero
39 Error in Photometer Repeat Errors 60 or 61
and Abort
40 Error in filters or photometer Repeat Reference

and Abort channel energy
less than 10%
and frequency
level
41 Excessive energy in filter Reference Lamp intensity changed.
channel, more Usually this is a symptom that
than 110% of lamp is close to burning-out
reference
frequency
42 Burn Out Lamp or defective Abort Reference Burned out lamp. Change lamp. Verify if failure occurs
photometer frequency less Light is not reaching sensors. always at a given filter
than 2 times Defective photometer
zero level for a
given filter
44 Low energy in filter X Message Reference Normal lamp intensity variation None. Calibrate at the end of the run
frequency 50% along its life
to 90% of
calibration value
45 Insufficient energy in filter X Repeat Reference Unusual lamp intensity variationCalibrate. Change lamp. Check
and Abort frequency photometer aligning
between 2 times
noise level and
50% of
calibration value
46 Error in calibration Message
47 Internal error 1 in dilution Abort

50 Internal Error Abort Un consistent parameter No. 5. Parameter Pasos_CommMA
values. (service manual, 3.11) doesn't coincide
with twice Air gap volume in Internal Use
Parameters. Default: 10 and 5,
respectively
51 Error, table
and frequency
level
52 Syringe jammed Repeat ST1-3 (E22) Stops before completing the Check screw, lubricate, if necessary.
and Abort required volume Check motor and belt.
53 No Answer Repeat ST1-2 (E22) No answer from syringe moduleInspect CAVRO module connections.
and Abort Replace, if necessary
54 Wrong parameters Repeat ST0-6 Attempt to go beyond syringe

and Abort limit. (Manual operations)
57 Wrong CRC Repeat ST0-7 Defective communication. Check connectors, serial ports, cables
and Abort Reception not equal to
transmission.
58 Wrong CRC Memory Abort ST0-4 Defective parameters in the With auxiliary board, modify any
instrument boards parameter. This resets all CRC's. Next,
return to the original parameter.
59 Inexistent command Abort ST0-5 Wrong model selection
60 Error Converter Repeat ST1-3
and Abort
61 Error Filter wheel Repeat ST1-2
and Abort
62 Internal Parameters Repeat ST1-1

and Abort
Message: Only in Operating conditions and Messages windows

Stop: Big message in screen waiting for operator's action
Abort: operation permanently stops after a number of retrays
7 VALIDATION PROGRAM FOR PARAMETER TESTING
The TEST program is used to check analyzer DataPro operating parameters.

When performed by authorized personnel, it will be an official validation of
instrument’s specifications.
The access to it is through Miscellaneous > Test.
This validation test should be performed after installation, after servicing, by client
request, or periodically, approximately every 6 months.
109
7.1 REQUIRED ELEMENTS.
Solution a: Potassium chromate, Reagent grade, diluted in water to 5 gr/l, in water

Solution b: Potassium chromate, Reagent grade, diluted in water to 2 gr/l, in water
Solution c: Potassium chromate, Reagent grade, diluted in water to 0,150 gr/l, in
water
Solution d: Sodium Nitrite, Reagent grade, 50 gr/l in water
Solution e: This solution is the wash solution described in 3.3, with Wiener lab.
TW AA in a concentration of 2 drops/liter.
Solutions a, b and d are included in Calibration Set
7.2 DESCRIPTION OF TESTS

For a detailed description of the system tests, please review the instrument
user manual.
7.3 AUTOMATED VALIDATION TESTING

By pressing button of Batch, all tests will be performed in sequence.
To do that, solutions must be located at fixed positions generated when the Default
button is pressed:
Minimum required
Position 1: Solution b 250 µl
Position 2: Solution a 400 µl
Position 3: Solution d 300 µl
Position 23: Distilled water 1 ml
Reagent Position 1: Solution e 15 ml
(See section 7.1)
Operation:
1. Put 80 NEW cuvettes in reaction tray
2. Select the default conditions.
3. Put solutions b, a, d and e in the specified positions
4. Press Batch button
5. At the end, get the generated report.
110
8 COMMON SERVICING SITUATIONS
8.1 CALIBRATION INTERPRETATION
Older calibrations will be recorded in the errors file log.

1. Check minimum and maximum in Ref. Frequency
Differences of less than 2000 counts indicate steady operation
Differences between 2000 and 10000 could indicate lamp instability'.
Perform Test 1. Check contacts. Change lamp
2. Check gains and counts.
Gain of 31 and Reference counts of less than 50000 indicate poor energy/
Change lamp.
Clean optical system
Change preamplifier board.
Check lamp mounting assembly
More than one energy equal to 31 indicate defective lamp
3. Check zeros
Values above 5000 indicate faulty or ill-adjusted preamplifier
Repeat calibration
Readjust zeros
Change preamplifier.
8.2 MESSAGES RELATED TO ENERGY
Energy is compared in every reading with last calibration values for the
corresponding filter and reference channel.
The situation is as follows:
39 Error in Photometer. No signal. Frequency 0

40 Error in filters or photometer Energy less than 10%
41 Excessive energy in filter
44 Low energy in filter X 75% of calibration value
45 Insufficient energy in filter X
42 Burn Out Lamp or defective photometer
0 2N 50% 75% 110%
39 42 40 45 44 NORMAL 41
1. MESSAGE 41 OF EXCESSIVE ENERGY IS MANY TIMES RELATED TO THE

LAMP NEAR BURNOUT.
111
IF AFTER CALIBRATION IN THE NEXT FEW HOURS MESSAGE OF
EXCESSIVE ENERGY REPEATS, CHANGE LAMP.
2. Calibrate instrument. Compare with older calibrations. Check for any increase or
decrease of gain greater than one step.
if minimum and maximum values differ more than 10000, check filter wheel:
a) Mechanical touch or rubbing between filters holders and body.
b) Too much warping of the filter wheel
c) Defective sensor
d) Check if COMU Time_Motor parameter is 150.
8.3 VERTICAL AND HORIZONTAL ERRORS TOGETHER.

Check in error file multiple automatic initialization of both, vertical and horizontal.
Check for continuity in arm probe flat cable. Replace.
8.4 PROBE STOPS BEFORE REACHING OR DOES NOT STOP AT ALL.
1. Clean tip with a cotton tip wetted with hypochlorite solution.

2. Check if needle holder is not wet. Dry with hot air.
3. Check high frequency connector and cable.
4. Replace electronic unit. (2300-P199)
8.5 PROBLEMS IN HYDRAULIC SYSTEM.

For hydraulic system testing and maintenance, see section 5.6
8.6 CTS TIMEOUT MESSAGE.

This message indicates that communication between PC and instrument is
defective: the Clear To Send bit is not received. There are many causes that
produce this error. The important matter is to separate problem in three parts: PC,
cable and instrument.
1. Check for loose connections or defective cable. Measure continuity.

2. Check instrument power supply of +13.8 volts. The led DL5 in P155 board must
be ON. In this board voltage can be checked in pins 8 and 9 of connector J7.
This power supply goes down to 0 if short circuit protection acts whenever a fan is
defective.
3. Check PC serial port RS232C. Utilize loop back connector and test self
response of software. In "Internal Use Parameters" disable answer check and in
"Factory Parameters" disable "Use of diluter and Photometer". This way you
simulate instrument operation. If simulation operates, PC serial port is OK.
8.7 MESSAGES RELATED TO MECHANICAL PROBLEMS

Messages related to mechanical problems in Reaction tray, sample tray or probe
arm are in general related to faulty sensor system:
• Check for actual mechanical problems: sensor obstructed, sensor touching
tray, too close, too far, etc.
112
• Check for electrical connection of sensors.
• Replace sensor, if necessary.
8.8 UNDEFINED MESSAGES RELATED TO DATA BASES AND/OR

MEMORY.
It can happen if RAM or motherboard problems arise, messages related to
Databases, EDB engine or computer memory can be generated by the operating
system. They are external to the instrument software but one or more databases or
indexes can become damaged or corrupt. Same situation occurs if a sudden
voltage drop or power disconnection is produced.
The only solution in this case is the to rescue the software from older backup.
For this reason IT IS VERY IMPORTANT WHEN EXITING THE PROGRAM, IF
NO PROBLEMS ARE AT SIGHT, TO LET INSTRUMENT PERFORM AN AUTO-
MATIC BACKUP.
This way, the file Bu_X.ZIP will contain the whole directory \Auto_A, compacted.
When executing RECOVER, this compacted file will expand and replace the cur-
rent files in \Auto_A directory and \Auto_A\Data sub-directory..
Backup files Bu_1,zip, Bu_2.zip,… are generated Monday, Tuesday, etc.
8.9 CORRUPT INDEXES.

A corrupt database index occurs when electrical power shutoff or pulse happens
while information is stored in disk.
First identify the damaged database. More than one can be damaged. To identify
them, open all data and inspect items included in main menu:
(The names of databases are included as reference:
Methods Interferences
Methods in use Filters
Samples Calibrations
Historic Volumes
Trays in memory Priorities
Standards Times
When the damaged database is to be opened a message of corrupt index is dis-

played. The solution is to replace the database.
To do that, a complete copy of program and databases must be generated in other
directory than C:\Auto_A.
The complete database must be copied, including all files with the same name.
Example: If the database of priorities must be replaced, copy the files: priors.db;
priors.px; priors.val; priors.xg0; priors.yg0. (See section 5.2)
If no individual damaged database is detected, the whole must be recovered by
latest undamaged Bu_x.zip file recovery.
8.10 ALL READINGS OF SAMPLE AND OR REFERENCE CHANNEL ARE

ZERO.
If this happens in both channels, check power supplies.
113
If only one channel is faulty, check continuity in cable and inspect connector fixing
and assembly in both ends.
114
8.11 POOR DRYING ACTION; BLOCK DIRTY; BLOCK BREAKS EASILY.
Drying action depends on several things:

1. Correct aspiration in preceeding cycles
2. Accurate volume delivery in cuvette wash,
3. Correct vacuum system in drying block.
Perform washer test and adjust volume to a value around 550 microliters.
Block breaks when a cuvette is pulled up by the drying block. Be sure that cuvette
retain cover is in position at all times.
New cleaning block automatic procedure should prevent from staining drying block.
Nevertheless, if it stains , put about 500 microliters of water in a cuvette,
disconnect drying pump or stop it and send washer unit down. Leave block
immersed in water for about 15 minutes. Do not use cleaning solution or any other
corrosive agent.
115
9 ILLUSTRATIONS AND SCHEMATICS.
Front view of instrument

Back view of instrument
PROBE A RM
PROBE A RM
PROTECTION
(RO TA RY)
REA CTION CHA M BER

COV ER
PROBE
W A SH STA TIO N
SA M PLE A N D
M A IN S SW ITC H REA GEN T TRA Y
REM OV A B LE COV ER.

A CCESS TO DILUTER A N D
PERISTA LTIC PUM P 23GLV4Fi- r0
Front view of instrument (model without cuvette washer)

C OOL IN G FA N S OU TL ET
( KEEP U N OB STRU C TED )
FU SE HOL D ER
L A M P HOU SIN G M A IN S C ON N EC TION

SERIA L PO RT
C ON N EC T TO PC TO W A SH SOL U TION
LEV EL SEN SOR
TO W A IST B OTTL E (YEL LO W TU BE)
TO W A IST B OTTL E W A SH SO LU TIO N

L EV EL SEN SOR IN TA KE
(YEL L OW TU BE) 2 3 GL V 4 Ti- V 0
Back view of instrument (model without cuvette washer)

Front Panel detail
Capillary probe heater connection
Syringe Replacement
# This tube connects to the cuvette washing system. See schematics M24A30. Nr. Component Part Nr. Order Nr.
1 Intake tube, PVC Ø6xØ3x1400mm MGPV0306 MGPV0306
2 Peristaltic pump tubing M24M64 M24M64
3 Pump-Diluter tube with fittings M24H17A M24H17A
# 2
4 Diluter-Heater tube with fittings M24H17B M24H17B
5 Diluter syringe, CAVRO725030 VOSB1202 VOSB1202
3 4
6 Probe drain funnel M24H01 M24H01
7 Reaction chamber funnels (Qty:2) M24H19 M24H19
8 1/4"Y barbed connector VACOP06Y VACOP06Y
9 Drain hoses Ø11xØ6x3m MGS10611 MGS10611
10 Drain reservoir with stopper M24H08W M24H08W
11 Wash solution reservoir with stopper M24H07W M24H07W
12 Reservoir mesh filter replacement M24H03 M24H03
PROBE ARM
PUMP
5 6
1
DILUTER
8 7
9
11
12 10 NOTE: Avoid loops in drain hoses

to prevent funnel flooding
Layout of sample handling system.
M24A28, rev.3
Hydraulic sampling circuit

Lateral cover removal for lamp replacement
Lamp replacement
REM OVE SCREW S
REM OVE COVER
ACCESS TO LAM P
LODGING
23GLV4Li- v0
Removal of side cover for lamp replacement

(model without cuvette washer)
LAMP
ASSEMBLY
Part Nr. VA000LAM
KNURLED NUT
Part Nr. M24F28
Order Nr.
PHOTOMETER
REMOVE KNURLED NUT AND

1 REMOVE LAMP ASSEMBLY
REMOVED LAMP COVER

2 UNPLUG LAMP CONNECTOR
3 REINSTALL NEW LAMP

ASSEMBLY
PLUG-IN LAMP
4 CONNECTOR
5 SCEW LAMP COVER IN PLACE

AND REPLACE SIDE PANEL
23GLV4LAi-v0
Lamp replacement
(model without cuvette washer)
Pump tubing assembly.
View of reaction cuvettes when set in light path.
Sample detector unit, reaction tray removed.
Peristaltic pump tube replacement
W as he r u ni t m ou nti n g ho l es
R ea c ti on tra y
R ea c ti on c ham ber
W A S H U NIT D IS AS SE MBL Y, (p lu s mo d e l onl y).
(N O T E: Th is v ers i on a ppl i es t o i ns t rum en ts w i th s e ri al n um ber 0 406 24x x and hi ghe r) .
Barcode label positioning in sample vial
Peristaltic pump tube replacement
CPU Board
PC boards support frame
Main PC Boards
Robot shaft
Diluter and dispenser unit
Robot unit
Connector to mains Photometer
Power supplies Reaction tray
Cuvette washer unit

(optional)
Control panel Reaction chamber
Reagent and sample

tray motor
ISE Unit,
To remove cabinet (optional)
unscrew 3 m6 hex. socket
screws at lower part of
lateral frames. Then Cooled reagent tray Reagent cooling
lift cabinet carefully off. (cooler optional) system fan
IMPORTANT!: do not
unscrew from base plate. VIEW WITH CABINET REMOVED
View with cabinet removed

M6 hex. socket
screws
Reaction tray assy.
Photometer
Instrument frame
Photometer removal
Photometer removal
Cuvette
Condensing lens
Sample detector
Side View
Intreference filter
Beamsplitter
Diaphragm
Lamp
Upper View
Collimating lens
Condensing lens
Reference detector
PHOTOMETER, OPTICAL LAYOUT
M24A20, rev.0
Photometer optical layout

Condensing lens
Collar #M24F12
Condensing lens holder
Beam-splitter and holder
#M24F16W
Reference detector and

preamplifier housing
Filter wheel position sensor

and PCB Lamp cover
Sample detector and

preamplifier housing
Filter wheel motor

#OT005017
Condenser frame
Frame
Collar
Collimating lens
Filter wheel
#M24F13
#M24F02W
Lamp assy. fixing
nut, (knurled),#M24F28 Lamp assembly
#VA000LAM
PHOTOMETER, PART #M24F00W, FRONT EXPLODED VIEW.
M24A23, rev.0
Photometer front exploded view

#M24F02, (filters not included)
#M24F02W, (filters included)
Filter and filter holder,

#M24F02B-[wavelength]
NOTE: Filter Wheel need not be removed from Photometer for filter changes.
This configuration applies to instruments with serial number 01072301
Filter replacement to Photometer Wheel
M24A25 rev.0
Photometer wheel - Filter replacement

Component Part #
1 PTFE tube and fittings M24H17A

2 Threaded fitting M24H13A
3 Female union M24H14
4 Filter assy. M24H02W
5 Tube bracket M24M33
6 Tension rod M24M36
7 Sleeve M24M37
17
8 Pressure spring M24M39
9 Knob M24M38
16 1 10 Pump rotor M24M31W
11 Bed M24M34
2 12 M3x8 hex. socket screw
15 Washer M24M35A
3 13
14 Bushing M24M35B
4 15 Pump tube M24M64
14
16 1/8"barbed fitting. VAX06365
5
17 Ø3xØ6 PVC tube MGPV0306
13 6 18 Pump motor (not visible) OT025CP1
7
12
8
11 9
10
PERISTALTIC PUMP, PART LIST.

Man 23
M24A33, rev.0
Peristaltic pump - Part list

Diluter
Switch panel Power supplies
Tray motor
clamping screws,
loosen to adjust
belt tension.
Remove fixing
screw to lower
Lower power supplies to access

diluter and pump
Remove 2 screws to free the switch panel

before lowering the power supplies
POWER SUPPLIES TILTED TO ACCESS FRONT PANEL COMPONENTS
Power supplies
Reaction tray driving belt
Reaction tray driving
pulley and motor
Photometer
Robot unit
Reaction tray assy.

(tray removed)
Reaction tray
position sensors
(not visible)
Reaction chamber heater

Fan
Heater unit support plate
Posts
Heater unit is removed
through the base plate
for servicing
View of Reaction Tray and Heater,

(reaction chamber partially removed).
View of reaction tray and heater

Reagent cooler fan
(Plus model only) Heater unit
Base plate of
instrument
Cross recessed screws
Removal of Heater unit and Cooling Fan

through Base Plate.
Removal of heater unit and cooling fan

Reaction tray assy.
Remove screws
before pulling
tray out
Belt
Pulley
Tray position sensor
board and support
bracket
Loosen screw to
slacken belt
before disassembly Motor Shaft fixing screw Frame
Reaction Tray Teardown
Reaction tray teardown

Remove 4 hex. socket screws to
disassemble tray to
Loosen motor bracket access Peltier cooler units.
screws for Soften conductive grease with
belt tension relief hot air gun before attempting
to lift the tray off.
Belt, loosen before

disassembling unit
Motor
Reagent tray, cooled
Position sensors Access to shaft set screw.

PC board Loosen to remove tray
Sensor board support assy from base frame
bracket. Remove
before pulling tray
assy. out
Frame
Cooling fan, remove
through base plate
Air duct
REAGENT TRAY DISASSEMBLY, (plus model only).
Reagent tray disassembly

Reagent tray cooler assy.
Loosen screw to
slacken belt
Colector rings
and brushes
Belt
Air duct
Tray position
sensors and
bracket. Remove Robot horizontal
before pulling tray movement motor
Access to shaft
off the frame.
fixing screw, (hex.
socket).
Frame
Reagent Tray Removal
Reagent tray removal

Probe elevation shaft
Lubricated felt whipper
Bearings
Adjustment set screw and nut
(to remove probe arm play. Do not
Guiding fork over tighten).
Guide shaft Sensor pc-board, vertical movement

Elevation belt
Elevation motor
Frame
Arm rotation belt
Arm rotation motor
Robot Assembly
Robot assembly
Robot arm assembly
Upper ring holder
Upper posts #M24S08
#M24S22
Removable sample and reagent

tray assy. #M24SB08W
Lower posts
#M24SB41
Lower ring holder
#M24SB36
Support plate
#M24SB37
Central post
#M24SB42
Platform
#M24SB35W
SAMPLE AND REAGENT TRAY ASSY. (Basic model).

M24A46, rev.0
Sample and reagent tray assembly

11
3
10
9
Probe home position
7
6
5
24
Home offset
4
23
Position 1
3
R
2
R-
S-R
set
set
Off
22
Off
Position 5
48
Position 4
Position 7 Position 3 Position 6
Position 2
PROBE POSITIONS AND TRAY OFFSETS
Probe positions and tray offsets

Washer unit mounting holes
Reaction tray
Reaction chamber
WASH UNIT DISASSEMBLY, (plus model only).

(NOTE: This version applies to instruments with serial number 040624xx and higher).

Manual Servicio cm200

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Wiener lab.

Automated Clinical Chemistry Analyzer

This manual applies to those instruments delivered

For technical assistance, please contact local representative or directly to

Warning: Before using read instructions in

Representative for CE:

Warranty is subject to the following conditions:

Trained personnel must perform installation.

Installation Checklist and Test Report must be sent to

1) Perform daily, weekly and quarterly maintenance procedures, as specified in user

WARNING: Instrument provided with cuvette washer

4 FILES AND DATABASES INCLUDED IN THE SYSTEM............................................81

2.12 ISE Module is not available in this model

The system supports the following modules:

Multiple Sample/Reagent Tray. Loads 48 samples. Each sample can be positioned

Reaction tray. 80 reaction well capacity. If the number of programmed reactions

Photometer. The photometer is provided with 9-interference filters mounted in a filter

Cleaning system. In between sample aspiration, a programmable peristaltic pump

1.2 OPERATING FEATURES

Factor Calculation: Determines how the factor is handled. As options, a previously

Statistics: Statistical analysis may be performed on samples, standards and

1.3 TECHNICAL SPECIFICATIONS

WARNING: Instrument is Installation Category II. Instrument

Icons. Most of them correspond to already defined menus.

Samples and Reagents Tray

Full initialization of system

Trays are disabled to facilitate load of samples and new cuvettes.

Ends all procedures in progress.

Start of automatic procedure.

Stops and resumes dispensing for Stat procedures

Times, volumes and priorities.

Prints active window

Please refer to user manual.

*: Might change depending the model of the instrument.

ITEM BOARD QUANTITY COMMENTS

SAMPLE VERTICAL HORIZONTAL

2.4 COMMUNICATION PROTOCOLS

2.4.1 COMMUNICATION PROTOCOL BETWEEN COMU AND DILUTER

All transmitted characters are ASCII.

1 START COMMAND BLOCK '/' 2Fh

The answer block is:

1 START ANSWER BLOCK '/' 2Fh

2.4.2 COMMUNICATION PROTOCOL BETWEEN COMU AND THE PC

COMU returns immediately the following message:

Three ASCII STX (002H) synchronism characters are used.

PHOTOMETER '0' - 030H

2.5 COMMANDS FROM PC TO THE COMU

2.5.1 PROBE ARM ROBOT

2.5.3 PHOTOMETER 990

2.5.4 REACTION TRAY

2.5.5 SAMPLE / REAGENTS TRAY

2.5.6 PERISTALTIC PUMP

2.6 DESCRIPTION OF THE MESSAGES

The message that PC sends to COMU, is:

/ * ROUTINE THE CRC CALCULATES * /

The controllers in Analyzer are only receivers.

• error of CRC 16 bits: the PC resends the command.

• busy peripheral: the PC waits.

2.7 COMMUNICATION PROTOCOL BETWEEN COMU AND CARRU

These commands are used by COMU to control CARU CPU.

• Sample/reagent tray initialization

• Probe, upper rest position

• Probe, to reagent position