THE Effects of Honey (Apis dorsata) Supplements on

Increased Bone Strength in Ovariectomized Rat

as Animal Model of Osteoporosis

1
Ira Sari Yudaniayanti*, 1Hardany Primarizky, 1Lianny Nangoi

1
Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas
Airlangga. Jl. Mulyorejo Kampus C unair 60115
*Korespondensi : irasari.vet@gmail.com

Abstract. Osteoporosis is a chronic skeletal disease characterized by low bone mass and microarchitectural deterioration
with a consequent increase in bone fragility and fracture risk. The aim of the study was to evaluate the effects of honey
(Apis dorsata) supplements on increased bone strength in ovariectomized rat as animal models of osteoporosis. Twenty
female rats at 3 months of age, weighing 150-200 g were used in the study. The rats were divided into five groups (n=4)
: Sham operation group (SH); ovariectomy group no treatment(OVX); ovariectomy with treatment Apis dorsata 1g/Kg BW
(AD-1); ovariectomy with treatment Apis dorsata 2g/Kg BW (AD-2); ovariectomy with treatment Apis dorsata 4g/Kg BW
(AD-3). The treatment started to be given the next day after ovariectomy operation for 12 weeks. The Rats were sacrified
within 12 weeks, and then the right femur were taken bone strength test. Based on the statistical analysis of the bone
strength test, the greatest score belongs to the Sham operation group (SH) that have significant difference (p<0.05) with
OVX group and AD-1 group, but there was no significant difference with AD-2 and AD-3 (p>0,05). In conclusion, honey
(Apis dorsata) supplements has the effect of increasing bone strength in ovariectomized rat as animal models of
osteoporosis, so that honey (Apis dorsata) supplements has the potential to be used as an alternative treatment for
osteoporosis.

Keywords : Osteoporosis, Honey (Apis dorsata), bone strength, ovariectomized rat