Polymorphism of G894T Gene Nitric oxide synthase 3 and nitric oxide levels in

type 2 diabetes with hypertension in the Bataknese

Jelimutiaraegar, University of Sumatera Utara, Indonesia.

Introduction : Diabetes mellitus (DM) is a chronic endocrine disorder characterized by

hyperglycemia with impaired metabolism of carbohydrates, fats and proteins resulting from
disorders of insulin hormone secretion, insulin function, or both. Hyperglycemia and other
metabolic changes can cause interference with the production of nitric oxide (NO). Nitric Oxide is
synthesized from L-Arginine by endothelial nitric oxide synthase (eNOS) encoded by the eNOS3
gene on chromosome 7. An important polymorphism described in the literature is that G894T
results in accelerated degradation and significantly reduces NO production which contributes to the
occurrence of atherosclerosis. Purpose : Proving the relationship between Nitric Oxide gene
polymorphism and Nitric Oxide levels in type 2 DM with hypertension in the Bataknese. Method :
A Case ontrol study was performed to evaluated the association between polymorphism of G894T
Gene and nitric oxide level. 56 consecutiv patients with type 2 diabetes with hypertension and 56
healty controls were recruited. The Genotypes polymorphism were determined by the polumerase
chain reacion-restriction fragment length polymorphism (PCR-RFLP) analysis. Result : the
Polymorphism of G894T Gene Nitric oxide synthase 3 was found to be assosiated with
hypertension in type 2 diabetes in the bataknese population as the patients group had higher
frequeny compared with the controls. The nitric oxide levels in type 2 diabetes with hypertension in
the Bataknese was lower than controls. Conclusion : The result of this study indicated that the
G894T polymorphism in NOS 3 may be genetic susceptibility factor for hypertension in the
bataknese population.

Biography

Jelita Sumitra has completed her Master at the age of 32 years and Speciality of Clinical Pathology
at the age 33 years from Universitas Sumatera Utara and Medical Doctor studies from Universitas
Sumatera Utara. She is the secretary of the clinical pathology at Universitas Sumatera Utara. She
has published 3 papers in reputed journals and has been serving as an Co-Author in 2 papers in
reputated journals.