BIOSAFETY

Ghulam Rasool
Lecturer Department of Allied
Health Sciences, SMC
Biosafety
“ Laboratory biosafety”: Describes
• containment principles,
• technologies &
• practices
that are implemented to prevent unintentional exposure
to pathogens & toxins, or their accidental release
“Laboratory biosecurity”: refers to
• institutional measures
• personal security measures
designed to prevent the loss, theft, misuse, diversion/intentional
release of pathogens & toxins
 Waste
ROAD TO BIOSAFETY Management

Containment
levels

Donning & Doffing PPE,s

Recapping needles

Hand washing
HAND WASHING TECHNIQUE

Biosafety protect you

from harmful incidents
OOPs!
You may get infection when
the hands are not washed
properly

Protect yourself: Wash your hands

 Why Should we Wash our Hands?

• Washing your hands correctly is the most

effective thing you can do to protect yourself
against a number of infectious disease, such
as influenza (the "flu") and the common cold.
Not only will it keep you healthy, it will help
prevent the spread of infectious diseases to
others.
• The CDC says you need to wash your hands-
with soap for at least 20 seconds in order to
kill disease-causing germs. However the
average person only washes their hands for a
minimum of 6 seconds.
 How to wash your hands?
• Wet hands with water, Apply single shot of soap
1. Rub hands palm to palm
2. Rub back of each hand with the palm of other hand with fingers
interlaced
3. Rub palm to palm with fingers interlaced
4. Rub with backs of fingers to opposing palms with fingers interlocked
5. Rub each thumb clasped in opposite hand using rotational
movement
6. Sweep hand from finger to thumb
7. Rub tips of fingers in opposite palm in circular motion
8. Rub each wrist with opposite hand
• Rinse hands with water, use elbow to turn off tap, Dry each hand
with a separate tissue paper, Now your hands are clean
HAND WASHING STEPS
STOP!
RECAPPING
WITH BOTH
HANDS IS A
DANGEROUS
PRACTICE

If possible, dispose of needles immediately

without recapping them
 Is Recapping Dangerous?

• Recapping needles is extremely dangerous because

it can result in accidental punctures of the finger or
hand, which can lead to potential exposure to
hazardous chemicals or infectious biological agents.
 Recapping Needles

• Do not recap needles for disposal whenever

possible.

• If recapping is required for the procedure being

done, you must use tongs, a recapping device or
one-hand scope method to recap the needle.
One Hand Scoop Technique

• In case, if recapping is unavoidable

use One hand scoop technique
 One Hand Scoop Technique

• Method for capping needles

• that involves placing the needle cover on a flat
surface, then inserting the needle into it without
touching needle or cover
• intended to prevent accidental needle-stick
 DOFFING OF GLOVES: Beak Method
Be careful when you are doffing your gloves
b/c your gloves are contaminated
Beak method

Hand contamination
Vehicle of pathogen
transmission

Beaking method
Effective strategy of
glove removal

Who developed?
Sean G. Kaufmann
(2006)
Steps of Beaking method
Step 1
Form a “L

Step 7
Step 2
Remove the
Give me Five
beaked glove

Step 6
Step 3
Pull first glove
Pinch and scoop
off

Step 5
Step 4
Pull the Glove
Form a Beak
over the Beak
 Steps of Beaking method

1 2 3 4

5 6 7
BEAKING METHOD STEPS

1. Form a “L”
2. Give me five.
3. With index finger and thumb – pinch and scoop
with your middle finger.
4. Form a Beak
5. Pull the glove over the Beak
6. Remove glove
7. With index finger – remove other gloves.