INHIBITION TEST OF HONEY EXTRACT

Apis mellifera BEE AGAINST THE GROWTH OF Propionibacterium acnes

Libert Valentine* Amanah ** Iwan Hermawan**

*college student of medical faculty swadaya gunung jati university
** lecturer of medical faculty swadaya gunung jati university

BACKGROUND METHODS
Acne vulgaris is the biggest skin The study was an experimental
diseases in the world with laboratory with post-test control group
prevalence 70-87% design. The Propionibacterium acnes
Propionibacterium acnes is a gram grown on blood nutrient agar. Honey
positive baceria that causes acne
Apis mellifera bee diluted with 5
concentrations 25%, 50%, 75%, 85%
vulgaris. To reduce the bacterial
and 100% and 2 control groups, (+)
resistance towards antibiotic, then
control with Clindamycin and (-)
developed alternative medication control with DMSO. Incubated for 24
with natural ingridients that have hours then inhibition zone formed was
an antibacterial effect, once of measured by a ruler.
them is HONEY

RESULT
AIM The results obtained from this study
This study aim to determine the
showed the average of inhibition
inhibitory of honey extract Apis against Propionibacterium acnes, on
mellifera bee to Propionibacterium positive control with clindamycin
acnes growth. (44 mm) has the largest inhibition
zone among concentration of 100%
was 8,33mm, 85% was 4,67mm, CONCLUSION
negative control with DMSO was
4,67mm, 50% was 2,67mm, 75% was There is an inhibition of
REFERENCES
3mm and 25% was 1,33mm. Honey extract Apis
1. Wu, Q. Antimicrobial Effect of Hanuka Honey and

mellifera bee against

15
Manuka Honey Alone and In Combination With The
Bioactives Against The Growth of Propionibacterium acnes
ATCC 6918. New Zealand: Massey University; 2011.
2. Diniati, J. Daya Hambat Madu Sumbawa Terhadap
Propionibacterium acnes
10

Pertumbuhan Kuman Staphylococcus aureus Isolat Infeksi

Luka di RS Islam Amal Sehat Sragen. Surakarta: Universitas
Sebelas Maret, Fakultas Kedokteran; 2010 5
3. White, R., Cooper, R., & Molan, P. Honey: A Modern
Wound Management Product. Wiltshire: Wounds UK
Publishing; 2005
0
Clindamycin 100% 85% 75% 50% 25% DMSO