MOLECUL AR SPECTROSCOPY
Product Specif ications
Thermo Scientific Evolution 300
UV-Vis Spectrophotometer
Accuracy, precision and reliability for results that matter
The Thermo Scientific™ Evolution™ 300
UV-Visible (UV-Vis) spectrophotometer
is designed to meet the challenges
and requirements of the life science,
material science, pharmaceutical,
quantitative analysis, and spectroscopy
laboratory.
The Thermo Scientific Evolution 300
spectrophotometer continues the tradition of
high-performance, reliability, and exceptional
value that have become the hallmark of Thermo
Scientific UV-Vis spectrophotometers. The
instrument’s stable double beam optical design
delivers high-quality data for the complete
lifetime of the instrument. Thermo Scientific™
Smart Accessories™, application-driven soft-
ware, and user configuration choices provide
an unmatched solution to fit your budget.
Innovative Sampling
Smart Accessories offer a sophisticated level
of automation that makes measuring your
sample easy. The plug-and-play design provides
quick interchange of sampling accessories,
saving time and increasing productivity. When
a Smart Accessory is installed the instrument
automatically recognizes it by serial number
which assists with meeting GLP and regulatory
requirements. Evolution spectrophotometers
support an extensive selection of accessories
for the measurement of almost any sample
type including solids, liquids, and diverse
sample sizes and compositions.
Less Downtime, More Data
The Evolution 300 spectrophotometer is
designed for labs requiring high performance,
outstanding reliability and a minimum of
down-time.
• Instant-on performance – there is no need to
wait for the lamps to warm up and stabilize.
Get accurate, stable measurements from
the moment that the instrument is turned on.
• No time lost changing lamps – the xenon
flash lamp typically lasts for 5 years or more.
– Three year lamp warranty insulates you from
operating expenses.
–N  o time lost to burn in new lamps or to re-qualify
your instrument after each lamp change.
–N  o service costs for system qualification and
verification.
• C hoose an optional Calibration and Validation
Carousel (CVC) to automate all your routine
performance verification tasks. Just insert
the CVC, select your method and walk away.
The report is ready for inspection and filing
in your log when you return.
Software Support
Comprehensive and powerful software
completes your Evolution 300 system.
• Thermo Scientific™ VISIONpro™ software
offers sophisticated tools for data acquisition,
analysis and reporting in general research
and quality control environments.
• V ISIONsecurity™ software offers advanced
security options and complete tools for
achieving 21 CFR Part 11 compliance.
• Thermo Scientific™ VISIONlife™ software
adds pre-programmed methods for
performing routine assays, such as nucleic
acid tests and protein concentrations.
Advanced capabilities include life science
kinetics methods and temperature based
rate for DNA melting experiments.
• Choose VISIONlite™ ColorCalc to measure
and calculate color parameters for
transmitting materials.
 Specifications

Product Specif ications

Optical Design Modified Ebert
Double beam with sample and reference cuvette/accessory positions
Spectral Bandwidths Variable 0.5, 1.0, 1.5, 2.0, 4.0 nm
Light Source Xenon Flash Lamp
Typical Lifetime: >5 years. Longer if not using live signal.
Warranty Period: 3 year source replacement warranty
Detector Dual Matched Silicon Photodiodes
Grating Holographic, 1200 lines/mm, blazed at 240 nm
Beam Separation 210 mm
Scan Ordinate Modes Absorbance, % Transmittance, % Reflectance, Concentration,
1st–4th Derivative
Wavelength Range 190–1100 nm
Accuracy ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm
Repeatability Standard deviation of 10 measurements <0.05 nm
Scanning Speeds 3800, 2400, 1200, 600, 240, 120, 60, 30, 10, 5, 1 nm/min
Intelliscan
Data Interval 10, 5, 2, 1, 0.5, 0.2, 0.1, 0.05 nm
Photometric Range >4 A
Display ±6 A
Readout Absorbance, % Transmission, % Reflectance, Concentration
Accuracy – Instrument1 1A: ±0.004 A
2A: ±0.004 A
3A: ±0.006 A
Accuracy – 1A: ±0.008 A
Including Standard Tolerance2 2A: ±0.010 A
3A: ±0.018 A
Noise 0A: <0.00018 A
1A: <0.00022 A
2A: <0.00050 A
500 nm, 2.0 nm SBW, RMS
Repeatability 1A: ±0.0025 A
Drift <0.0005 Abs/hour, 500 nm, 2.0 nm SBW, 2 hr warm-up
Stray Light 198 nm: 2.9 A KCl
220 nm: 4.2 A NaI
340 nm: 4.3 A NaNO2
Baseline Flatness ±0.0015 A (200–800 nm)
2.0 nm SBW, smoothed
Dimensions (W × D × H) 61 × 53 × 38 cm
Weight 22 kg
Electrical Supply 100–240 V, 50–60 Hz

Pharmacopoeia Compliance Testing

Resolution (Toluene in Hexane) Peak/Trough Ratio >2.0
Photometric Accuracy (60mg/L K 2Cr 2O7 ) <±0.01 A relative to calibrated sealed standard
Stray Light <0.13 %T at 198 nm with KCl per EP
220 nm: <0.01%T NaI (behaves identically to KI for USP)
Wavelength Accuracy ±0.20 nm (546.11 nm Hg emission line), ±0.30 nm 190–900 nm
Wavelength Repeatability Peak separation of repetitive scanning of Hg line source <0.10 nm
1

Measured at 546 nm using neutral density filters traceable to NIST/NPL 2

Measured at 546 nm using neutral density filters traceable to NIST/NPL
Instrument tolerance is calculated by determining total tolerance with the Total Tolerance is the root sum square of the uncertainty in the filter
uncertainty in the filter calibration removed from the calculation calibration and Instrument Tolerance

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