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UCSD BIBC 102 Practice Midterm
UCSD BIBC 102 Practice Midterm
UCSD BIBC 102 Practice Midterm
Lkklkkkkkkkkkkl;
1. Fill in the following table about the amino acids Serine and Cysteine in the
dipeptide shown above.
Property Serine Cysteine
Classification
Present in Chymotrypsin
(Yes/No)
2. Circle the amino acid side chain (R group) that could receive a phosphate group
from a protein kinase.
4. For a chemical reaction where the equilibrium constant (Keq) is much greater than
one.
a. What sign is the ΔG’o
d. How would a catalyst affect the equilibrium constant for the reaction?
5. How do exergonic reactions actually power endergonic reactions in the body? Use
the example of Glutamine biosynthesis in your response.
Practice Exam for Midterm
a. What features of the Chymotrypsin active site account for its specificity?
b. What features of the enzyme active site account for its catalytic ability? Describe
the general sequence for proteolysis of a peptide.
d. During which step(s) of Glycolysis does ADP accept a phosphate group from a
high energy carbon-phosphate intermediate?
9. You find an unknown compound labeled “Chemical X.” To determine the identity
of the compound, you test it with a sample of enzyme and take kinetic data before and
after the addition of the unknown compound. What class of compound is Chemical
X?
Practice Exam for Midterm
b. Hexokinase
d. Monoamine oxidase
e. Aldolase
.
f. Alcohol Dehydrogenase
h. Trypsin
i. Proteases
14. When substrate binding activates the enzyme, this is an example of:
15. A sigmoid, or ‘S’ shaped Michaelis-Menten plot of V0 vs. [S], is typically seen
with which of the following:
16. Label the above graph with: the axes, Vmax, ½ Vmax and indicate which Vo position is
equivalent to KM (do your best to arbitrarily position these labels).
17. What are the assumptions used to derive the graph above?
18. Write the general equation that best describes the Michaelis-Menten graph:
19. Draw a Lineweaver Burke plot for an enzyme in the presence and absence of an irreversible
inhibitor.
Written by Alex Vu, Tony Yu, Ben Lewin, Mandi Ma, Dr. Aaron
Coleman and Sam Rana during Summer Session II 2011