Professional Documents
Culture Documents
Chapter Iii
Chapter Iii
Chapter Iii
contains the research design, chemicals and reagents, plant extraction, phytochemical screening,
the procedures used in the study and the statistical analysis used in the treatment of data.
Research Design
The design used by the researchers in the study is the experimental design. With this
method, the researchers have the ability to study the variables to find the answer to the stated
problem and to analyze the potentiality of Aloe vera and its phytochemical components.
Research Study
This study seeks to investigate the use of experimental design towards discovering the
phytochemicals in Aloe vera against type 2 diabetes. The plant sample, Aloe vera gel, will be
obtained within Tandag City. Phytochemical screening is used to determine if Aloe vera contains
Sampling Design
The sampling design used in this research is applying the non-probability purposive
sampling design. It means that the plant material, Aloe vera gel, was purposefully sought for and
was tested for the specific use of this research. The materials used were gathered from Tandag
All chemicals and reagents used were of analytical grade and were obtained from and
Collected 100 grams of fresh Aloe Vera from Tandag City Public Market which were
then washed and cut. The outer coating was then sliced and the gel inside was extracted. Then it
Twenty microliters of extract were dispensed into each sterile paper disk. The
impregnated paper disk was placed in a sterile petri dish and air dried for 1 hour under the
Phytochemical Analysis
The gel extract was subjected to qualitative phytochemical screening for the presence of
some chemical constituents such as steroids, phenolics, fixed oil, alkaloid, glycoside, saponin,
flavonoid, tannins and carbohydrate. Investigation for the presence of the phytoconstituents
activities of Aloe vera. The analyzation was used to determine the presence of certain chemical
constituents.
Conceptual Framework
Statistical Analysis
This study used One-way Analysis of Variance to analyze and study the given results.
The Tukey’s multi-comparison test was then used to compare the following effectiveness
between the various treatments that were found in the phytochemical screening of Aloe vera gel
Diabetes Mellitus).